Sustained enhancement of photosynthesis in coffee trees grown under free-air CO2 enrichment conditions: disentangling the contributions of stomatal, mesophyll, and biochemical limitations.


:Coffee (Coffea spp.), a globally traded commodity, is a slow-growing tropical tree species that displays an improved photosynthetic performance when grown under elevated atmospheric CO2 concentrations ([CO2]). To investigate the mechanisms underlying this response, two commercial coffee cultivars (Catuaí and Obatã) were grown using the first free-air CO2 enrichment (FACE) facility in Latin America. Measurements were conducted in two contrasting growth seasons, which were characterized by the high (February) and low (August) sink demand. Elevated [CO2] led to increases in net photosynthetic rates (A) in parallel with decreased photorespiration rates, with no photochemical limitations to A. The stimulation of A by elevated CO2 supply was more prominent in August (56% on average) than in February (40% on average). Overall, the stomatal and mesophyll conductances, as well as the leaf nitrogen and phosphorus concentrations, were unresponsive to the treatments. Photosynthesis was strongly limited by diffusional constraints, particularly at the stomata level, and this pattern was little, if at all, affected by elevated [CO2]. Relative to February, starch pools (but not soluble sugars) increased remarkably (>500%) in August, with no detectable alteration in the maximum carboxylation capacity estimated on a chloroplast [CO2] basis. Upregulation of A by elevated [CO2] took place with no signs of photosynthetic downregulation, even during the period of low sink demand, when acclimation would be expected to be greatest.


J Exp Bot


DaMatta FM,Godoy AG,Menezes-Silva PE,Martins SC,Sanglard LM,Morais LE,Torre-Neto A,Ghini R




Has Abstract


2016-01-01 00:00:00














  • Reporter gene expression reveals precise auxin synthesis sites during fruit and root development in wild strawberry.

    abstract::The critical role of auxin in strawberry fruit set and receptacle enlargement was demonstrated previously. While fertilization is known to trigger auxin biosynthesis, the specific tissue source of fertilization-induced auxin is not well understood. Here, the auxin reporter DR5ver2::GUS was introduced into wild strawbe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Feng J,Dai C,Luo H,Han Y,Liu Z,Kang C

    更新日期:2019-01-07 00:00:00

  • PpEG4 is a peach endo-beta-1,4-glucanase gene whose expression in climacteric peaches does not follow a climacteric pattern.

    abstract::In peach (Prunus persica L. Batsch.) the degradation of the pectic compounds of the cell wall is considered to be the principal component responsible for fruit softening. Many genes encoding enzymes acting on the different polymers of the pectic matrix have been shown to be highly expressed during the late phases of s...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Trainotti L,Pavanello A,Zanin D

    更新日期:2006-01-01 00:00:00

  • Multifaceted roles of nitric oxide in tomato fruit ripening: NO-induced metabolic rewiring and consequences for fruit quality traits.

    abstract::Nitric oxide (NO) has been increasingly implicated as part of the ripening regulatory networks in fleshy fruits. However, very little is known about the simultaneous action of NO on the intertwined web of regulatory events and metabolic reactions behind the ripening-related changes in fruit color, taste, aroma and nut...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zuccarelli R,Rodríguez-Ruiz M,Lopes-Oliveira PJ,Pascoal GB,Andrade SCS,Furlan CM,Purgatto E,Palma JM,Corpas FJ,Rossi M,Freschi L

    更新日期:2020-11-09 00:00:00

  • Cloning and functional comparison of a high-affinity K+ transporter gene PhaHKT1 of salt-tolerant and salt-sensitive reed plants.

    abstract::To understand the mechanisms of ion homeostasis in salt-tolerant and salt-sensitive plants, cDNAs for a high-affinity K(+) transporter PhaHKT1 were isolated from salt-sensitive (Utsunomiya) and salt-tolerant (Nanpi, Enchi) reed plants. A cDNA of Utsunomiya (PhaHKT1-u) contained two insertions in the region correspondi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Takahashi R,Liu S,Takano T

    更新日期:2007-01-01 00:00:00

  • Aquaporin-mediated increase in root hydraulic conductance is involved in silicon-induced improved root water uptake under osmotic stress in Sorghum bicolor L.

    abstract::The fact that silicon application alleviates water deficit stress has been widely reported, but the underlying mechanism remains unclear. Here the effects of silicon on water uptake and transport of sorghum seedlings (Sorghum bicolor L.) growing under polyethylene glycol-simulated osmotic stress in hydroponic culture ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Liu P,Yin L,Deng X,Wang S,Tanaka K,Zhang S

    更新日期:2014-09-01 00:00:00

  • Extracellular beta-glucosidase activity in barley involved in the hydrolysis of ABA glucose conjugate in leaves.

    abstract::Abscisic acid conjugate concentrations increased in barley xylem sap under salinity, whereas it remained at a low level in the intercellular washing fluid (IWF) of barley primary leaves (Hordeum vulgare cv. Gerbel). Here it is shown that IWF contains beta-glucosidase activity which releases abscisic acid (ABA) from th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Dietz KJ,Sauter A,Wichert K,Messdaghi D,Hartung W

    更新日期:2000-05-01 00:00:00

  • Analysis of potential redundancy among Arabidopsis 6-phosphogluconolactonase isoforms in peroxisomes.

    abstract::Recent work revealed that PGD2, an Arabidopsis 6-phosphogluconate dehydrogenase (6-PGD) catalysing the third step of the oxidative pentose-phosphate pathway (OPPP) in peroxisomes, is essential during fertilization. Earlier studies on the second step, catalysed by PGL3, a dually targeted Arabidopsis 6-phosphogluconolac...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lansing H,Doering L,Fischer K,Baune MC,Schaewen AV

    更新日期:2020-01-23 00:00:00

  • Sugar regulation of SUGAR TRANSPORTER PROTEIN 1 (STP1) expression in Arabidopsis thaliana.

    abstract::Sugars regulate the expression of many genes at the transcriptional level. In Arabidopsis thaliana, sugars induce or repress the expression of >1800 genes, including the STP1 (SUGAR TRANSPORTER PROTEIN 1) gene, which encodes an H(+)/monosaccharide cotransporter. STP1 transcript levels decrease more rapidly after the a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cordoba E,Aceves-Zamudio DL,Hernández-Bernal AF,Ramos-Vega M,León P

    更新日期:2015-01-01 00:00:00

  • Uptake of glycine by non-mycorrhizal Lolium perenne.

    abstract::Plants of Lolium perenne were grown in sterile solution culture. 15N-labelled glycine (Gly) coupled with gas chromatograph mass spectrometry was used to prove that non-mycorrhizal plants of L. perenne are capable of acquiring N in the form of intact Gly. It was estimated that a minimum of 80% of Gly-N uptake, over a 3...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Thornton B

    更新日期:2001-06-01 00:00:00

  • The molecular characterization of PaHB2, a homeobox gene of the HD-GL2 family expressed during embryo development in Norway spruce.

    abstract::PaHB1 (for Picea abies Homeobox1), an evolutionarily conserved HD-GL2 homeobox gene, specifically expressed in the protoderm during somatic embryogenesis in the gymnosperm Norway spruce has been reported previously. An additional HD-GL2 gene designated PaHB2 is reported here. During somatic embryogenesis, the PaHB2 ge...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ingouff M,Farbos I,Wiweger M,von Arnold S

    更新日期:2003-05-01 00:00:00

  • Local root ABA/cytokinin status and aquaporins regulate poplar responses to mild drought stress independently of the ectomycorrhizal fungus Laccaria bicolor.

    abstract::The relatively better performance of mycorrhizal plants subjected to drought stress has commonly been linked to improved root water uptake through the fungal regulation of plant aquaporins and hormones. In this study, we examined the role of ectomycorrhizal fungi in plant water relations and plant hormonal balance und...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Calvo-Polanco M,Armada E,Zamarreño AM,García-Mina JM,Aroca R

    更新日期:2019-11-18 00:00:00

  • Regulation of the calcium-sensing receptor in both stomatal movement and photosynthetic electron transport is crucial for water use efficiency and drought tolerance in Arabidopsis.

    abstract::Production per amount of water used (water use efficiency, WUE) is closely correlated with drought tolerance. Although stomatal aperture can regulate WUE, the underlying molecular mechanisms are still unclear. Previous reports revealed that stomatal closure was inhibited in the calcium-sensing receptor (CAS) antisense...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Wang WH,Chen J,Liu TW,Chen J,Han AD,Simon M,Dong XJ,He JX,Zheng HL

    更新日期:2014-01-01 00:00:00

  • Open and closed inflorescences: more than simple opposites.

    abstract::The absence of a terminal flower in inflorescences ('open inflorescences') is currently explained by the maintenance of putative stem-cells in the central zone (CZ) of the inflorescence meristem (IM) governed by the CLAVATA-WUSCHEL regulatory loop. Disruption of this regulatory pathway, as in Arabidopsis TERMINAL FLOW...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Bull-Hereñu K,Classen-Bockhoff R

    更新日期:2011-01-01 00:00:00

  • How agro-ecological research helps to address food security issues under new IPM and pesticide reduction policies for global crop production systems.

    abstract::Drivers behind food security and crop protection issues are discussed in relation to food losses caused by pests. Pests globally consume food estimated to feed an additional one billion people. Key drivers include rapid human population increase, climate change, loss of beneficial on-farm biodiversity, reduction in pe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: E Birch AN,Begg GS,Squire GR

    更新日期:2011-06-01 00:00:00

  • The alpha-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana.

    abstract::Heterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the G alpha subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Okamoto H,Göbel C,Capper RG,Saunders N,Feussner I,Knight MR

    更新日期:2009-01-01 00:00:00

  • Functional roles of melatonin in plants, and perspectives in nutritional and agricultural science.

    abstract::The presence of melatonin in plants is universal. Evidence has confirmed that a major portion of the melatonin is synthesized by plants themselves even though a homologue of the classic arylalkylamine N-acetyltransferase (AANAT) has not been identified as yet in plants. Thus, the serotonin N-acetylating enzyme in plan...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Tan DX,Hardeland R,Manchester LC,Korkmaz A,Ma S,Rosales-Corral S,Reiter RJ

    更新日期:2012-01-01 00:00:00

  • Temperate flowering phenology.

    abstract::Individuals, families, networks, and botanic gardens have made records of flowering times of a wide range of plant species over many years. These data can highlight year to year changes in seasonal events (phenology) and those datasets covering long periods draw interest for their perspective on plant responses to cli...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Tooke F,Battey NH

    更新日期:2010-06-01 00:00:00

  • Turgor, temperature and the growth of plant cells: using Chara corallina as a model system.

    abstract::Rapid changes in turgor pressure (P:) and temperature (T:) are giving new information about the mechanisms of plant growth. In the present work, single internode cells of the large-celled alga Chara corallina were used as a model for plant growth. P was changed without altering the chemical environment of the wall whi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Proseus TE,Zhu GL,Boyer JS

    更新日期:2000-09-01 00:00:00

  • Fluxes in central carbohydrate metabolism of source leaves in a fructan-storing C3 grass: rapid turnover and futile cycling of sucrose in continuous light under contrasted nitrogen nutrition status.

    abstract::This work assessed the central carbohydrate metabolism of actively photosynthesizing leaf blades of a C3 grass (Lolium perenne L.). The study used dynamic (13)C labelling of plants growing in continuous light with contrasting supplies of nitrogen ('low N' and 'high N') and mathematical analysis of the tracer data with...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lattanzi FA,Ostler U,Wild M,Morvan-Bertrand A,Decau ML,Lehmeier CA,Meuriot F,Prud'homme MP,Schäufele R,Schnyder H

    更新日期:2012-03-01 00:00:00

  • The biosynthesis of phenolic acids is positively regulated by the JA-responsive transcription factor ERF115 in Salvia miltiorrhiza.

    abstract::Phenolic acids are important secondary metabolites produced in the Chinese medicinal plant Salvia miltiorrhiza, but little is known about the transcription factors involved in the regulation of tanshinone and phenolic acid biosynthesis. Here, a novel AP2/ERF transcription factor SmERF115 was isolated and functionally ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sun M,Shi M,Wang Y,Huang Q,Yuan T,Wang Q,Wang C,Zhou W,Kai G

    更新日期:2019-01-01 00:00:00

  • Nitric oxide signalling in plant interactions with pathogenic fungi and oomycetes.

    abstract::Nitric oxide (NO) and reactive nitrogen species have emerged as crucial signalling and regulatory molecules across all organisms. In plants, fungi and fungi-like oomycetes, NO is involved in the regulation of multiple processes during their growth, development, reproduction, responses to the external environment and b...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Jedelská T,Luhová L,Petřivalský M

    更新日期:2020-12-24 00:00:00

  • Expression of FRIGIDA in root inhibits flowering in Arabidopsis thaliana.

    abstract::FRIGIDA (FRI), as the major regulator of flowering time in Arabidopsis accessions, can activate its target FLOWERING LOCUS C (FLC) to delay flowering before vernalization. In addition to FLC, other FRI targets also exist in Arabidopsis. Although leaves sense environmental cues to modulate flowering time, it is not kno...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kong X,Luo L,Zhao J,Chen Q,Chang G,Huang J,Yang Y,Hu X

    更新日期:2019-10-15 00:00:00

  • Effect of activase level and isoform on the thermotolerance of photosynthesis in Arabidopsis.

    abstract::Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) activation decreases under moderate heat stress. This decrease is caused by an impairment of activase function, which is exacerbated by faster rates of Rubisco deactivation at elevated temperatures. To determine if stromal oxidation causes inhibition of activas...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Salvucci ME,DeRidder BP,Portis AR Jr

    更新日期:2006-01-01 00:00:00

  • Dwarf alleles differentially affect barley root traits influencing nitrogen acquisition under low nutrient supply.

    abstract::Sustainable food production depends critically on the development of crop genotypes that exhibit high yield under reduced nutrient inputs. Rooting traits have been widely advocated as being able to influence optimal plant performance, while breeding-based improvements in yield of spring barley suggest that this specie...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Karley AJ,Valentine TA,Squire GR

    更新日期:2011-07-01 00:00:00

  • Drought stress modulates cuticular wax composition of the grape berry.

    abstract::Drought events are a major challenge for many horticultural crops, including grapes, which are often cultivated in dry and warm climates. It is not understood how the cuticle contributes to the grape berry response to water deficit (WD); furthermore, the cuticular waxes and the related biosynthetic pathways are poorly...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Dimopoulos N,Tindjau R,Wong DCJ,Matzat T,Haslam T,Song C,Gambetta GA,Kunst L,Castellarin SD

    更新日期:2020-05-30 00:00:00

  • Salinity-induced reduction in root surface area and changes in major root and shoot traits at the phytomer level in wheat.

    abstract::The aim of this study was to investigate the effect of salinity stress on root growth at the phytomer level in wheat to provide novel site-specific understanding of salinity damage in roots. Seedlings of 13 wheat varieties were grown hydroponically. Plants were exposed to three concentrations of NaCl, 0 (control), 50 ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Robin AH,Matthew C,Uddin MJ,Bayazid KN

    更新日期:2016-06-01 00:00:00

  • Physiological and genetic analysis of Arabidopsis thaliana anthocyanin biosynthesis mutants under chronic adverse environmental conditions.

    abstract::Anthocyanin production is a characteristic response of flowering plants to unfavourable environmental conditions. The potential roles of flavonoids and anthocyanins in plant growth were investigated by growing Arabidopsis thaliana anthocyanin production mutants (transparent testa) under limiting nitrogen and high ligh...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Misyura M,Colasanti J,Rothstein SJ

    更新日期:2013-01-01 00:00:00

  • The interaction of strigolactones with abscisic acid during the drought response in rice.

    abstract::Both strigolactones (SLs) and abscisic acid (ABA) biosynthetically originate from carotenoids. Considering their common origin, the interaction of these two hormones at the biosynthetic and/or regulatory level may be anticipated. Here we show that, in rice, drought simultaneously induces SL production in the root, and...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Haider I,Andreo-Jimenez B,Bruno M,Bimbo A,Floková K,Abuauf H,Ntui VO,Guo X,Charnikhova T,Al-Babili S,Bouwmeester HJ,Ruyter-Spira C

    更新日期:2018-04-23 00:00:00

  • Effect of anthocyanins, carotenoids, and flavonols on chlorophyll fluorescence excitation spectra in apple fruit: signature analysis, assessment, modelling, and relevance to photoprotection.

    abstract::Whole apple fruit (Malus domestica Borkh.) widely differing in pigment content and composition has been examined by recording its chlorophyll fluorescence excitation and diffuse reflection spectra in the visible and near UV regions. Spectral bands sensitive to the pigment concentration have been identified, and linear...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Merzlyak MN,Melø TB,Naqvi KR

    更新日期:2008-01-01 00:00:00

  • Three differentially expressed basic peroxidases from wound-lignifying Asparagus officinalis.

    abstract::The activity of ionically bound peroxidases from an asparagus spear increased from 5-24 h post-harvest. Isoelectric focusing showed that the post-harvest increase of the total peroxidase activity was due to the increase of several distinct isoperoxidases. Concomitantly, a decrease in the activity of two anionic peroxi...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Holm KB,Andreasen PH,Eckloff RM,Kristensen BK,Rasmussen SK

    更新日期:2003-10-01 00:00:00