Abstract:
:This study presents the characterization of the plasma membrane (PM) H+-ATPases in potato, focusing on their role in stolon and tuber development. Seven PM H+-ATPase genes were identified in the Solanum tuberosum genome, designated PHA1-PHA7. PHA genes show distinct expression patterns in different plant tissues and under different stress treatments. Application of PM H+-ATPase inhibitors arrests stolon growth, promotes tuber induction, and reduces tuber size, indicating that PM H+-ATPases are involved in tuberization, acting at different stages of the process. Transgenic potato plants overexpressing PHA1 were generated (PHA1-OE). At early developmental stages, PHA1-OE stolons elongate faster and show longer epidermal cells than wild-type stolons; this accelerated growth is accompanied by higher cell wall invertase activity, lower starch content, and higher expression of the sucrose-H+ symporter gene StSUT1. PHA1-OE stolons display an increased branching phenotype and develop larger tubers. PHA1-OE plants are taller and also present a highly branched phenotype. These results reveal a prominent role for PHA1 in plant growth and development. Regarding tuberization, PHA1 promotes stolon elongation at early stages, and tuber growth later on. PHA1 is involved in the sucrose-starch metabolism in stolons, possibly providing the driving force for sugar transporters to maintain the apoplastic sucrose transport during elongation.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Stritzler M,Muñiz García MN,Schlesinger M,Cortelezzi JI,Capiati DAdoi
10.1093/jxb/erx284subject
Has Abstractpub_date
2017-10-13 00:00:00pages
4821-4837issue
17eissn
0022-0957issn
1460-2431pii
4085270journal_volume
68pub_type
杂志文章abstract::Transcription factors are proposed as suitable targets for the control of traits such as yield or food quality in plants. This study reports the results of a functional genomics research effort that identified ATHB17, a transcription factor from the homeodomain-leucine zipper class II family, as a novel target for the...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ert261
更新日期:2013-11-01 00:00:00
abstract::The plant family Cactaceae is considered among the most threatened groups of organisms on the planet. The threatened status of the cacti family has created a renewed interest in the highly evolved physiological and morphological traits that underpin their persistence in some of the harshest subtropical environments in...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erz320
更新日期:2019-11-29 00:00:00
abstract::The effects of synchronous photo (16 h daylength) and thermo (2 degrees C daily fluctuation) cycles on flowering time were compared with constant light and temperature treatments using two barley mapping populations derived from the facultative cultivar 'Dicktoo'. The 'Dicktoo'x'Morex' (spring) population (DM) segrega...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ern131
更新日期:2008-01-01 00:00:00
abstract::Pectins are major components of plant primary cell walls. They include homogalacturonans (HGs), which are the most abundant pectin and can be the target of apoplastic enzymes like pectin methylesterases (PMEs) that control their methylesterification level. Several PMEs are expressed in the seed coat of Arabidopsis tha...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw025
更新日期:2016-04-01 00:00:00
abstract::Brassinosteroids (BRs) are a group of steroid hormones regulating plant growth and development. Since BRs do not undergo transport among plant tissues, their metabolism is tightly regulated by transcription factors (TFs) and feedback loops. BAS1 (CYP734A1, formerly CYP72B1) and SOB7 (CYP72C1) are two BR-inactivating c...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz468
更新日期:2020-01-23 00:00:00
abstract::Grain number per spike, which is greatly influenced by floret fertility, is an important trait of wheat (Triticum aestivum L.) yield. Maximum floret primordia, fertile floret, and final grain number per spikelet are three crucial factors of floret fertility. Floral degradation plays a critical role in determining thes...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv303
更新日期:2015-09-01 00:00:00
abstract::The increasing occurrence of heatwaves has intensified temperature stress on terrestrial vegetation. Here, we investigate how two contrasting isoprene-emitting tropical species, Ficus benjamina and Pachira aquatica, cope with heat stress and assess the role of internal plant carbon sources for isoprene biosynthesis in...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz353
更新日期:2019-10-24 00:00:00
abstract::Mosses are among the earliest branching embryophytes and probably originated not later than the early Ordovician when atmospheric CO2 was higher and O2 was lower than today. The C3 biochemistry and physiology of their photosynthesis suggests, by analogy with tracheophytes, that growth of extant bryophytes in high CO2 ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw012
更新日期:2016-03-01 00:00:00
abstract::Rice grain filling is a process of conversion of sucrose into starch catalysed by a series of enzymes. Sucrose synthase (SUS) is considered as a key enzyme regulating this process. This study investigated the possible roles of sucrose and abscisic acid (ABA) in mediating the activity and expression of SUS protein of g...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp114
更新日期:2009-01-01 00:00:00
abstract::Transgenic tobacco (Nicotiana tabacum cv. LA Burley 21) lines expressing three genes encoding enzymes thought to be critical for the efficient production of phytochelatins, (i) serine acetyltransferase (EC 2.3.1.30) involved in the production of O-acetylserine, the cysteine precursor, (ii) gamma-glutamylcysteine synth...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj176
更新日期:2006-01-01 00:00:00
abstract::The effect of the complex relationship between ethylene and abscisic acid (ABA) on flower development and senescence in Hibiscus rosa-sinensis L. was investigated. Ethylene biosynthetic (HrsACS and HrsACO) and receptor (HrsETR and HrsERS) genes were isolated and their expression evaluated in three different floral tis...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err218
更新日期:2011-11-01 00:00:00
abstract::Global change factors affect plant carbon uptake in concert. In order to investigate the response directions and potential interactive effects, and to understand the underlying mechanisms, multifactor experiments are needed. The focus of this study was on the photosynthetic response to elevated CO(2) [CO2; free air CO...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err133
更新日期:2011-08-01 00:00:00
abstract::Growing potato tubers or freshly harvested mature tubers have a dormant apical bud. Normally, this dormancy is spontaneously broken after a period of maturation of the tuber, resulting in the growth of a new sprout. Here it is shown that in in vitro-cultured growing and maturing tubers, ethanol can rapidly break this ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri245
更新日期:2005-09-01 00:00:00
abstract::The geometry of leaf margins is an important shape characteristic that distinguishes among different leaf phenotypes. Current definitions of leaf shape are qualitative and do not allow quantification of differences in shape between phenotypes. This is especially true for leaves with some non-trivial three-dimensional ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru062
更新日期:2014-05-01 00:00:00
abstract::The determinism of bud bursting pattern along the 1-year-old shoot was studied at the molecular and morphological levels in the apple tree variety 'Lodi' which shows an acrotonic tendency. At the molecular level, the expression of KNAP2, which belongs to the class I KN1-like gene family, was studied. Measurements were...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erf063
更新日期:2002-11-01 00:00:00
abstract::Mutants sensitive to ionizing radiation in yeast and mammals include an assortment of DNA repair genes. The majority of these DNA repair genes are involved in the repair of DNA double-strand breaks. In this study a forward genetic screen is used to identify gamma-sensitive mutants of Arabidopsis thaliana. The gamma-pl...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg069
更新日期:2003-02-01 00:00:00
abstract::Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signalling network for improving productivity in crop species. This first requires identifying components of the conserved network and their function in...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa495
更新日期:2020-10-24 00:00:00
abstract::Because it has a high demand for sulphur (S), oilseed rape is particularly sensitive to S limitation. However, the physiological effects of S limitation remain unclear, especially during the rosette stage. For this reason a study was conducted to determine the effects of mineral S limitation on nitrogen (N) and S upta...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq096
更新日期:2010-06-01 00:00:00
abstract::Wood formation in trees is a dynamic process that is strongly affected by environmental factors. However, the impact of ozone on wood is poorly documented. The objective of this study was to assess the effects of ozone on wood formation by focusing on the two major wood components, cellulose and lignin, and analysing ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err047
更新日期:2011-06-01 00:00:00
abstract::By using a fine oil-filled glass microcapillary mounted on a micromanipulator, the solutes of individual plant cells can be sampled. These samples can then be analysed using a range of physical and chemical methods. Hydrostatic pressure (cell pressure probe), osmotic pressure (picolitre osmometer), organic solutes (en...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:
更新日期:2001-04-01 00:00:00
abstract::The genomes of many plant viruses have a coding capacity limited to <10 proteins, yet it is becoming increasingly clear that individual plant virus proteins may interact with several targets in the host for establishment of infection. As new functions are uncovered for individual viral proteins, virologists have reali...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erv520
更新日期:2016-03-01 00:00:00
abstract::Rapid changes in turgor pressure (P:) and temperature (T:) are giving new information about the mechanisms of plant growth. In the present work, single internode cells of the large-celled alga Chara corallina were used as a model for plant growth. P was changed without altering the chemical environment of the wall whi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.350.1481
更新日期:2000-09-01 00:00:00
abstract::Nutrient requirements for plant growth are expected to rise in response to the predicted changes in CO(2) and temperature. In this context, little attention has been paid to the effects of soil temperature, which limits plant growth at early stages in temperate regions. A factorial growth-room experiment was conducted...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/52.362.1913
更新日期:2001-09-01 00:00:00
abstract::Proanthocyanidins (PAs) are agronomically important biopolymers in higher plants composed primarily of catechin and epicatechin units. The biosynthesis of these natural products is regulated by transcription factors including proteins of the R2R3MYB class. To gain insight into the genetic control of the catechin and e...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erq344
更新日期:2011-01-01 00:00:00
abstract::Receptor kinases play important roles in plant growth and development, but only few of them have been functionally characterized in depth. Over the past decade CRINKLY 4 (CR4)-related research has peaked as a result of a newly discovered role of ARABIDOPSIS CR4 (ACR4) in the root. Here, we comprehensively review the a...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw192
更新日期:2016-08-01 00:00:00
abstract::This work introduces a new experimental method for the comprehensive description of the physiological responses to light of photosynthetic organisms. It allows the integration in a single experiment of the main established manipulative chlorophyll fluorescence-based protocols. It enables the integrated characterizatio...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw492
更新日期:2017-02-01 00:00:00
abstract::Photo-oxidative stress was imposed under natural solar radiation on exposed and shaded sections of detached fruit of immature green tomato (Lycopersicon esculentum Miller = Solanum lycopersicum L.) mutants (anthocyanin absent, beta-carotene, Delta, and high pigment-1) and their nearly isogenic parents ('Ailsa Craig' a...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj136
更新日期:2006-01-01 00:00:00
abstract::Stem rust, caused by Puccinia graminis (Pg), remains a devastating disease of wheat, and the emergence of new Pg races virulent on deployed resistance genes fuels the ongoing search for sources of durable resistance. Despite its intrinsic durability, non-host resistance (NHR) is largely unexplored as a protection stra...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery066
更新日期:2018-04-27 00:00:00
abstract::Medicago truncatula is a model legume plant that interacts symbiotically with Sinorhizobium meliloti, the alfalfa symbiont. This process involves a molecular dialogue between the bacterium and the plant. Legume roots exude flavonoids that induce the expression of a set of rhizobial genes, the nod genes, which are esse...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh261
更新日期:2004-12-01 00:00:00
abstract::Dihydroflavonol 4-reductase (DFR) is the first committed enzyme of the anthocyanin and condensed tannin pathways. Several DFR cDNAs have been cloned, and different specificities of DFR isozymes in the substrate hydroxylation patterns have been reported, but only fragmentary knowledge of DFR gene organization is availa...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri251
更新日期:2005-09-01 00:00:00