Abstract:
BACKGROUND:Pollens represent a rich source of proteins that are also potential elicitors of IgE-mediated pollen allergy. Sensitisation to panallergens could play an important role in diagnosis and specific immunotherapy, because these molecules are present in different plant pollens and plant foods and have marked structural similarity in different species. Profilins are one of the most common panallergens to be studied because they are responsible for a large number of sensitisations and are clearly related to cross-reactivity and co-sensitisation. This study aimed to isolate and characterise a new allergen of Amaranthus palmeri pollen and to determine its allergenicity. METHODS:A. palmeri pollen profilin was purified using poly-l-proline-Sepharose affinity chromatography followed by anion exchanger chromatography. Identification of purified protein was carried out by mass spectrometry. Specific IgE was estimated in sera of patients with positive skin prick test to A. palmeri pollen extract, by enzyme-linked immunosorbent assay (ELISA). PRINCIPAL FINDINGS:Purified protein appeared as a single band at 14 kDa in SDS-PAGE gel. Mass spectrometric analysis of the gel band identified two highly conserved peptides corresponding to allergenic profilins from pollen of other plants. Sera from about 60% of allergic patients have IgE that recognises the purified A. palmeri protein. CONCLUSION:A 14 kDa protein of A. palmeri pollen was purified and identified as allergenic profilin, which was recognised by sera from pollen allergic patients.
journal_name
Allergol Immunopathol (Madr)journal_title
Allergologia et immunopathologiaauthors
Landa-Pineda CM,Arroyo-Becerra A,Rosas-Alvarado A,Terán LM,Garcia-Cruz ML,Marchat LA,Reyes-López CAdoi
10.1016/j.aller.2015.05.002subject
Has Abstractpub_date
2016-03-01 00:00:00pages
160-6issue
2eissn
0301-0546issn
1578-1267pii
S0301-0546(15)00098-1journal_volume
44pub_type
杂志文章abstract:BACKGROUND:Children with IgE-mediated cow's milk allergy (IgE-CMA) often tolerate baked milk within a wheat matrix. In our study we evaluated the impact of wheat matrix and of little standardised cooking procedures on tolerance of baked milk. We also tested tolerance versus parmigiano reggiano (PR) and whey-based parti...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2016.03.005
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Anaphylaxis is an acute multisystemic and potentially fatal reaction, resulting from the rapid release of inflammatory mediators. Its exact prevalence is unknown. In children, foods are the most significant triggers for IgE-mediated anaphylaxis. OBJECTIVES:To characterise the cases of anaphylaxis evaluated ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2010.12.012
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:The pathogenesis of atopic dermatitis (AD) is still not completely understood. AD is characterized by the presence of clinical symptoms of both IgE antibody-mediated immediate hypersensitivity and specific T lymphocyte-mediated delayed hypersensitivity. OBJECTIVE:To evaluate the immunological mechanisms inv...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(04)79251-4
更新日期:2004-09-01 00:00:00
abstract::Immunotherapy delivered a new therapeutic option to the oncologist: Ipilimumab (anti-CTLA-4), Nivolumab and Pembrolizumab (anti-PD1), and Atezolizumab (anti-PD-L1) increase overall survival and show a better safety profile compared to chemotherapy in patients with metastatic melanoma, lung, renal cancer among others. ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1016/j.aller.2018.04.005
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abstract:BACKGROUND:The sharon fruit is the edible fruit of the persimmon tree (Diospyros kaki) which belongs to the Ebenaceae family. There are few references of allergic reaction to this fruit. We introduce a case of a 33-years old man with a anaphylactic reaction immediately after ingestion of sharon fruit. METHODS:Skin pri...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(01)79021-0
更新日期:2001-03-01 00:00:00
abstract::The present prospective, open, observational study assessed the effect of the use of standard environmental control measures together with the application of an acaricide-containing aerosol of esbiol/benzyl benzoate/piperonyl butoxide/2-phenylphenol (Frontac) on several clinical and environmental parameters in patient...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,多中心研究
doi:10.1016/s0301-0546(02)79096-4
更新日期:2002-03-01 00:00:00
abstract::Patients affected by cutinegative, RAST negative, chronic hyperreactive rhinitis showed in 15/28 cases positive intradermal tests, nasal provocation test and RAST on nasal fluids. In 13/28 cases high IgE on nasal samples could be observed and seldom were the intradermal tests positive, but in no case were the nasal pr...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::This study was motivated by the discrepancy in the results of published studies on the amount of histamine released following the intravascular administration of iodinated contrast media (ICM) in humans. From a group of patients due to undergo cardiac catheterization, we selected 45 subjects with no history of atopy. ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1157/13115668
更新日期:2008-01-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract::In medical literature, there are many cases reported of anaphylaxis due to fruits, before or after allergic reactions to latex. We present two atopic patients, with allergy to latex (by clinical history, positive skin test and RAST). Both patients showed a positive skin test and RAST to fruits (banana, kiwi, avocado a...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1157/13072924
更新日期:2005-03-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:
更新日期:1989-03-01 00:00:00
abstract::This study investigated the specificity and efficacy of immunotherapy with Artemisia vulgaris (Artemisia) extract. We studied 25 patients with a more than two year lasting history of seasonal rhinoconjunctivitis and who had only two clinically important allergies, either to Artemisia and Betula verrucosa (Betula) or t...
journal_title:Allergologia et immunopathologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:Autosomal dominant hyper-IgE syndrome (AD-HIES) is a primary immunodeficiency mainly caused by mutations in STAT3, a signalling molecule implicated in the development of appropriate immune responses. We aimed to characterise the innate immune response in AD-HIES. METHODS:The frequency of innate immune cells...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2014.11.002
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:Differences in latex allergen sensitization profiles have been described between children undergoing repeated surgical interventions and health care workers. The purpose of this study was to determine whether such sensitization profiles are associated with differences in the expression of latex allergen betw...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1157/13094028
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:Anaphylaxis is a sudden, severe, and potentially life-threatening allergic reaction, affecting a portion of allergic patients. Adrenaline is the first-line medication for anaphylaxis and available in many parts of the world as adrenaline autoinjectors (AAIs). OBJECTIVE:Aim of this study was to determine att...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2019.07.009
更新日期:2020-01-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2015.10.005
更新日期:2016-07-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(04)79240-x
更新日期:2004-07-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2010.06.008
更新日期:2011-07-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Excessive accumulation of histamine in the body leads to miscellaneous symptoms mediated by its bond to corresponding receptors (H1-H4). Increased concentration of histamine in blood can occur in healthy individuals after ingestion of foods with high contents of histamine, leading to histamine intoxication. In individ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1016/j.aller.2015.05.001
更新日期:2015-09-01 00:00:00
abstract::Impaired delayed type skin sensitivity is supposed to be due to abnormalities in T lymphocyte functions. Lately it has been assumed that T cells play an important part also in the development of late allergic reactions in the skin and in the lungs. Patients with sarcoidosis, lung carcinoma or with uremia were skin tes...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
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journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1157/13077746
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND:Approximately 150 species of Lepidoptera have been described as causing damage to human skin. One of these species is the pine processionary caterpillar, which is responsible for dermatitis, contact urticaria, ocular lesions and rarely respiratory signs and anaphylactic reactions through IgE-mediated or non-...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
abstract:BACKGROUND:Leukotrienes are among the most important mediators associated with inflammatory responses in patients with exercise induced asthma (EIA). The aim of this study was to investigate the impact of exercise on the urinary leukotriene profile. Hence, we compared post exercise changes of urinary leukotriene E4 (LT...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2011.03.012
更新日期:2012-05-01 00:00:00