Abstract:
BACKGROUND:Pollens represent a rich source of proteins that are also potential elicitors of IgE-mediated pollen allergy. Sensitisation to panallergens could play an important role in diagnosis and specific immunotherapy, because these molecules are present in different plant pollens and plant foods and have marked structural similarity in different species. Profilins are one of the most common panallergens to be studied because they are responsible for a large number of sensitisations and are clearly related to cross-reactivity and co-sensitisation. This study aimed to isolate and characterise a new allergen of Amaranthus palmeri pollen and to determine its allergenicity. METHODS:A. palmeri pollen profilin was purified using poly-l-proline-Sepharose affinity chromatography followed by anion exchanger chromatography. Identification of purified protein was carried out by mass spectrometry. Specific IgE was estimated in sera of patients with positive skin prick test to A. palmeri pollen extract, by enzyme-linked immunosorbent assay (ELISA). PRINCIPAL FINDINGS:Purified protein appeared as a single band at 14 kDa in SDS-PAGE gel. Mass spectrometric analysis of the gel band identified two highly conserved peptides corresponding to allergenic profilins from pollen of other plants. Sera from about 60% of allergic patients have IgE that recognises the purified A. palmeri protein. CONCLUSION:A 14 kDa protein of A. palmeri pollen was purified and identified as allergenic profilin, which was recognised by sera from pollen allergic patients.
journal_name
Allergol Immunopathol (Madr)journal_title
Allergologia et immunopathologiaauthors
Landa-Pineda CM,Arroyo-Becerra A,Rosas-Alvarado A,Terán LM,Garcia-Cruz ML,Marchat LA,Reyes-López CAdoi
10.1016/j.aller.2015.05.002subject
Has Abstractpub_date
2016-03-01 00:00:00pages
160-6issue
2eissn
0301-0546issn
1578-1267pii
S0301-0546(15)00098-1journal_volume
44pub_type
杂志文章abstract:BACKGROUND:Egg is the most frequent cause of food allergy in children. The bird-egg syndrome, found in a group of patients sensitized to egg through bird proteins, was infrequent in children. We report a patient with former history of hypersensitivity to egg who developed episodes of angioedema after ingestion of hen m...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(03)79164-2
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Differences in latex allergen sensitization profiles have been described between children undergoing repeated surgical interventions and health care workers. The purpose of this study was to determine whether such sensitization profiles are associated with differences in the expression of latex allergen betw...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1157/13094028
更新日期:2006-09-01 00:00:00
abstract::A 34-year-old woman referred episodes of gastrointestinal disorders and sometimes angioedema of the lips which appeared within 2 hours from the ingestion of packed food like biscuits, cereals or fruit juice. A patch test with the standard European series and with food preservatives was positive for fragrances mix. The...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1157/13112995
更新日期:2007-11-01 00:00:00
abstract::In this study we attempt to investigate if collagen type 1 from bovine Achilles tendon is capable of causing an inflammatory response. Thus, we immunized rats with a collagen emulsion injected in the right hind paw, via subplantar, and intradermally on the back. Seven days later, the rats were reimmunized by intraderm...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract:BACKGROUND:Gramineae bee-collected pollen is identified as being at the origin of allergic accidents but the biological potency of Gramineae bee-collected pollen is not well known. Cereal grasses (e.g., Zea) and European wild forage grasses (FG) are contained in bee-collected pollen. METHOD:In this experiment, Zea-mas...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2015.05.003
更新日期:2016-05-01 00:00:00
abstract::In 1975, Miller and Nussenzweig described a new function of the Complement (C'): the Capacity of this substance to solubilize "in vitro" antigen/antibody complexes previously formed. Subsequent studies of these authors and other researches (particularly Takahashi and his group) demonstrated that this process is mediat...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:
更新日期:1994-09-01 00:00:00
abstract::Five patients with atopic dermatitis, three males and two females, aged 2 to 17 years, had positive reactions to air allergens (Dermatophagoides pteronyssinus and/or farinae). All the patients suffered from severe recurrent dermatophytosis that responded poorly to antifungal treatment. The results of immunologic evalu...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(02)79135-0
更新日期:2002-09-01 00:00:00
abstract::An experimental model was devised in which an AKR lymphoma is conditioned to grow in BALB mice leading to reproducible tumor incidence which makes tumor-bearing (progressor) and tumor-rejecting (regressor) animals simultaneously available. The object of this paper was to determine the effect of neonatal thymectomy (xT...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract::In a 19 year old patient suffering from CMC since the first months of life, clinical improvement accompanied by correction of the immunologic defect was achieved by Transfer Factor therapy. After 12 months from the last administration of Transfer Factor the improvement persisted. The positive outcome of the treatment ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
abstract::The increasingly extensive use of selective serotonin reuptake inhibitors will probably increase the number of cases of cutaneous reactions due to these drugs. This report describes a case of generalized exanthema due to paroxetine. Positive results to patch tests indicated delayed hypersensitivity. ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(06)73527-3
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:Previous studies have shown that serum interleukin 33 serving as an "alarmin" is increased in children with asthma. The objective of this study was to assess the validity of serum IL33 test for early diagnosis of childhood asthma. METHODS:A literature search was performed in June 2016 using PubMed, Embase, ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.aller.2016.12.007
更新日期:2017-01-01 00:00:00
abstract::Local administration of drugs by means of aerosol device is widely used in the treatment of asthma. Nevertheless, the effectiveness of this method depends on an adequate inhalation technique (IT), which available evidence has shown to be rather problematic. The aim of this study was to assess IT in out-patients with b...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract:BACKGROUND:Since gamma interferon release assays (IGRAs) cannot differentiate between active tuberculosis and latent tuberculosis infection (LTBI), development of rapid and specific diagnosis tools are essential for discriminating between active tuberculosis (TB) from LTBI. Both IGRAs are based on Mycobacterium tubercu...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2015.09.004
更新日期:2016-07-01 00:00:00
abstract::This study was motivated by the discrepancy in the results of published studies on the amount of histamine released following the intravascular administration of iodinated contrast media (ICM) in humans. From a group of patients due to undergo cardiac catheterization, we selected 45 subjects with no history of atopy. ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Employing peripheral blood leucocytes as the model of the study, we proved how ketotifen blocks the inhibiting effect that the antigen induces over the adenyl cyclase activity in asthmatic individuals with sensitization to Dermatophagoides pteronyssinus. The strength of the Ketotifen effect is comparable to that produ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::The mechanisms of action of prophylactic antiallergic drugs, especially disodium cromoglycate (DSCG), have been reviewed. Some evidence is presented that suggests that DSCG acts not only as an inhibitor of mediator release from mast cells, but also by inhibiting the effects of mediators on bronchial and intestinal smo...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:
更新日期:1981-11-01 00:00:00
abstract::Some authors succeded in binding the bradykinin in form of a haptene by an azo-bridge to the human gammaglobulin as a vehicle. In this way, the bradykinin has been supplemented to a complete antigen. The bradykinin as a strongly vasoactive substance causes--after a local intradermal injection--an intensive circumscrib...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract:BACKGROUND:Approximately 150 species of Lepidoptera have been described as causing damage to human skin. One of these species is the pine processionary caterpillar, which is responsible for dermatitis, contact urticaria, ocular lesions and rarely respiratory signs and anaphylactic reactions through IgE-mediated or non-...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1157/13091043
更新日期:2006-07-01 00:00:00
abstract::Thanks to its excellent safety profile, sublingual immunotherapy has served as a basis for launching two important lines of research in current allergology: sublingual immunotherapy with pharmaceutical registry (oral lyophilizates or tablets), and sublingual immunotherapy with food. At present, clinical trials are bei...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(08)75223-6
更新日期:2008-09-01 00:00:00
abstract::Hypersensitivity reactions to aspirin and other NSAIDs occur in individuals genetically predisposed and exhibit different clinical manifestations, especially respiratory, cutaneous, and generalised. Five different phenotypes define distinct clinical pictures: aspirin-exacerbated respiratory disease, aspirin/NSAID cuta...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1016/j.aller.2016.08.010
更新日期:2017-01-01 00:00:00
abstract::Rhinomanometry is a technique which studies the resistance of the nasal airways. It also permits us to measure their variations during NPT. This work has analysed the different methods of rhinomanometry (previously active, previously passive and late), commenting on its advantages and disadvantages. Bearing in mind th...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::It is suggested that increased reactivity in asthma is not limited to the bronchi but also exists in the mediator releasing system. We have previously observed that the basophils of atopic asthmatics are degranulated by polymyxin B in vitro. The present study attempts to relate this basophil degranulation to atopy. Th...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::Impaired delayed type skin sensitivity is supposed to be due to abnormalities in T lymphocyte functions. Lately it has been assumed that T cells play an important part also in the development of late allergic reactions in the skin and in the lungs. Patients with sarcoidosis, lung carcinoma or with uremia were skin tes...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::Since half of the fetal transplantation antigens are paternal in origen, the conceptus could be considered as a particular allogeneic graft not rejected by the mother. The reason for this particular kind of tolerance remains mostly unknown, and an enormous amount of experimental work, clinical observations and hypothe...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
abstract:BACKGROUND:Asthma hospitalization rates in Chilean children have increased in the last 14 years, but little is known about the factors associated with this. OBJECTIVE:Describe clinical characteristics of children hospitalized for asthma exacerbation. METHODS:Observational prospective cohort study in 14 hospitals. Ove...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,多中心研究
doi:10.1016/j.aller.2018.02.006
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:One of the factors affecting compliance is the pharmaceutical formulation used. Many patients find it difficult to swallow tablets or capsules. The fast dissolving tablet (FDT) formulation could help to enhance patient compliance, because of its ease of administration and because no liquid is required to hel...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,多中心研究
doi:10.1157/13088176
更新日期:2006-05-01 00:00:00
abstract::Excessive accumulation of histamine in the body leads to miscellaneous symptoms mediated by its bond to corresponding receptors (H1-H4). Increased concentration of histamine in blood can occur in healthy individuals after ingestion of foods with high contents of histamine, leading to histamine intoxication. In individ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1016/j.aller.2015.05.001
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:The pathogenesis of atopic dermatitis (AD) is still not completely understood. AD is characterized by the presence of clinical symptoms of both IgE antibody-mediated immediate hypersensitivity and specific T lymphocyte-mediated delayed hypersensitivity. OBJECTIVE:To evaluate the immunological mechanisms inv...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(04)79251-4
更新日期:2004-09-01 00:00:00
abstract::We present the clinical case of a 8-years-old boy suffering a fixed drug reaction attributed to the oral intake of loratadine. He is an atopic child with perennial rhinitis and asthma and marked hypersensitivity to the house-dust mite Dermatophagoides pteronyssinus who is receiving inhaled corticosteroids and b2-agoni...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(03)79199-x
更新日期:2003-09-01 00:00:00
abstract:OBJECTIVE/HYPOTHESIS:We systematically reviewed the associations between allergic rhinitis or allergy and otitis media with effusion, by reference to published data. STUDY DESIGN:A meta-analysis of case-controlled studies. DATA SOURCE:Five databases (Pubmed, Highwire, Medline, Wanfang, and China National Knowledge In...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,meta分析
doi:10.1016/j.aller.2016.03.004
更新日期:2017-01-01 00:00:00