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Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos.

Abstract：

:Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both polyadenylated and non-polyadenylated RNAs from individual cells. This method exhibits robust sensitivity, precision and accuracy. We discover 2891 circRNAs and 913 novel linear transcripts in mouse preimplantation embryos and further analyze the abundance of circRNAs along development, the function of enriched genes, and sequence features of circRNAs. Our work is key to deciphering regulation mechanisms of circRNAs during mammalian early embryonic development.

journal_name

Genome Biol

journal_title

Genome biology

authors

Fan X,Zhang X,Wu X,Guo H,Hu Y,Tang F,Huang Y

doi

10.1186/s13059-015-0706-1

subject

Has Abstract

pub_date

2015-07-23 00:00:00

pages

148

eissn

1474-7596

issn

1474-760X

pii

10.1186/s13059-015-0706-1

journal_volume

16

pub_type

杂志文章

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