All Your Base: a fast and accurate probabilistic approach to base calling.

Abstract:

:The accuracy of base calls produced by Illumina sequencers is adversely affected by several processes, with laser cross-talk and cluster phasing being prominent. We introduce an explicit statistical model of the sequencing process that generalizes current models of phasing and cross-talk and forms the basis of a base calling method which improves on the best existing base callers, especially when comparing the number of error-free reads. The novel algorithms implemented in All Your Base (AYB) are comparable in speed to other competitive base-calling methods, do not require training data and are designed to be robust to gross errors, producing sensible results where other techniques struggle. AYB is available at http://www.ebi.ac.uk/goldman-srv/AYB/.

journal_name

Genome Biol

journal_title

Genome biology

authors

Massingham T,Goldman N

doi

10.1186/gb-2012-13-2-r13

subject

Has Abstract

pub_date

2012-02-29 00:00:00

pages

R13

issue

2

eissn

1474-7596

issn

1474-760X

pii

gb-2012-13-2-r13

journal_volume

13

pub_type

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