Abstract:
:The accuracy of base calls produced by Illumina sequencers is adversely affected by several processes, with laser cross-talk and cluster phasing being prominent. We introduce an explicit statistical model of the sequencing process that generalizes current models of phasing and cross-talk and forms the basis of a base calling method which improves on the best existing base callers, especially when comparing the number of error-free reads. The novel algorithms implemented in All Your Base (AYB) are comparable in speed to other competitive base-calling methods, do not require training data and are designed to be robust to gross errors, producing sensible results where other techniques struggle. AYB is available at http://www.ebi.ac.uk/goldman-srv/AYB/.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Massingham T,Goldman Ndoi
10.1186/gb-2012-13-2-r13subject
Has Abstractpub_date
2012-02-29 00:00:00pages
R13issue
2eissn
1474-7596issn
1474-760Xpii
gb-2012-13-2-r13journal_volume
13pub_type
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