Abstract:
:Many differential gene expression analyses are conducted with an inadequate number of biological replicates. We describe an easy and effective RNA-seq approach using molecular barcoding to enable profiling of a large number of replicates simultaneously. This approach significantly improves the performance of differential gene expression analysis. Using this approach in medaka (Oryzias latipes), we discover novel genes with sexually dimorphic expression and genes necessary for germ cell development. Our results also demonstrate why the common practice of using only three replicates in differential gene expression analysis should be abandoned.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Li Y,Yang H,Zhang H,Liu Y,Shang H,Zhao H,Zhang T,Tu Qdoi
10.1186/s13059-020-01966-9subject
Has Abstractpub_date
2020-03-23 00:00:00pages
66issue
1eissn
1474-7596issn
1474-760Xpii
10.1186/s13059-020-01966-9journal_volume
21pub_type
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