Abstract:
BACKGROUND/AIMS:The WNK-dependent STE20/SPS1-related proline/alanine-rich kinase SPAK participates in the regulation of NaCl and Na(+),K(+),2Cl(-) cotransport and thus renal salt excretion. The present study explored whether SPAK has similarly the potential to regulate the epithelial Na(+) channel (ENaC). METHODS:ENaC was expressed in Xenopus oocytes with or without additional expression of wild type SPAK, constitutively active (T233E)SPAK, WNK insensitive (T233A)SPAK or catalytically inactive (D212A)SPAK, and ENaC activity estimated from amiloride (50 µM) sensitive current (Iamil) in dual electrode voltage clamp experiments. Moreover, Ussing chamber was employed to determine Iamil in colonic tissue from wild type mice (spak(wt/wt)) and from gene targeted mice carrying WNK insensitive SPAK (spak(tg/tg)). RESULTS:Iamil was observed in ENaC-expressing oocytes, but not in water-injected oocytes. In ENaC expressing oocytes Iamil was significantly increased following coexpression of wild-type SPAK and (T233E)SPAK, but not following coexpression of (T233A)SPAK or (D212A)SPAK. Colonic Iamil was significantly higher in spak(wt/wt) than in spak(tg/tg) mice. CONCLUSION:SPAK has the potential to up-regulate ENaC.
journal_name
Kidney Blood Press Resjournal_title
Kidney & blood pressure researchauthors
Ahmed M,Salker MS,Elvira B,Umbach AT,Fakhri H,Saeed AM,Shumilina E,Hosseinzadeh Z,Lang Fdoi
10.1159/000368509subject
Has Abstractpub_date
2015-01-01 00:00:00pages
335-43issue
4eissn
1420-4096issn
1423-0143pii
000368509journal_volume
40pub_type
杂志文章abstract:AIMS AND METHODS:Our aim was to evaluate the antihypertensive efficacy and tolerability of angiotensin II type 1 (AT1) receptor antagonists. Valsartan or candesartan cilexetil was administered to 11 dialysis patients with elevated blood pressure. The patients (6 male, 5 female; mean age +/- SD 61+/-11 years) were on re...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000054209
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND/AIMS:IgA nephropathy is the most prevalent form of primary glomerulonephritis worldwide. Among patients with kidney disease, hypertension is one of the most important risk factors of disease progression. Considering the limited evidence regarding the appropriate blood pressure (BP) goal for patients with IgA...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,多中心研究
doi:10.1159/000495636
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND/AIMS:Peritoneal dialysis (PD) has gained interest over the last decade as a viable option for early start dialysis. It is still unknown if shorter break-in periods and less time for proper patient evaluation and training could influence technique survival in comparison to planned-start PD. METHODS:A prospec...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000495386
更新日期:2018-01-01 00:00:00
abstract::Fibroblast growth factor 23 (FGF23) is released primarily from osteoblasts/osteocytes in bone. In cooperation with the transmembrane protein Klotho, FGF23 is a powerful inhibitor of 1α 25OH Vitamin D Hydroxylase (Cyp27b1) and thus of the formation of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). As 1,25(OH)2D3 up-regulates ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000495393
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND/AIMS:We previously showed that urine and serum Angiopoietin-2 (Ang-2) levels increased significantly with the degree of albuminuria in diabetes patients, but the reasons remain unclear. Consequently we aimed to determine whether there was an association between Ang-2, inflammatory cytokines (TNF-α and IL-18)...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000368510
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND/AIMS:Additional validation study was warranted to confirm the clinical significance of C score, which was recently added to the Oxford classification for immunoglobulin A nephropathy (IgAN). METHODS:We performed a multicenter retrospective cohort study in four hospitals in Korea. Patients who had biopsied g...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,多中心研究
doi:10.1159/000497808
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:The contribution of preexisting hypercholesterolemia to diabetic nephropathy remains unclear. We assessed the impact of hypercholesterolemia on diabetic nephropathy using a double knockout (DKO) mouse, null for the low-density lipoprotein receptor (LDLRNDASH;/NDASH;) and the apoB mRNA editing catalytic ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000073942
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSC) improve renal function and renovascular hypertension in the 2-kidney 1-clip model (2K-1C). While MSC play an immunomodulatory role, induce neoangiogenesis, and reduce fibrosis, they do not correct sodium loss by the contra-lateral kidney. OBJECTIVES:We investigated the tubular f...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000503346
更新日期:2019-01-01 00:00:00
abstract::NGAL (neutrophil gelatinase-associated lipocalin) is an acute phase protein, participating in antibacterial immunity. NGAL forms a complex with metalloproteinase 9 (MMP-9), thereby increasing its activity and preventing its degradation. NGAL is freely filtered through the glomerular membrane and reabsorbed by endocyto...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000368474
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:The biological properties of oxidized and non-oxidized PTH are substantially different. Oxidized PTH (oxPTH) loses its PTH receptor-stimulating properties, whereas non-oxidized PTH (n-oxPTH) is a full agonist of the receptor. This was described in more than 20 well published studies in the 1970(s) and 80(s)....
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000350149
更新日期:2013-01-01 00:00:00
abstract::Recently, Chlamydia pneumoniae is the microorganism frequently implicated in the infection-based inflammatory atherogenous hypothesis. Although in vitro experimental data and initial sero-epidemiologic, pathology-based studies and antibiotic trials supported this interesting hypothesis, later data are conflicting. Som...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000078146
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND/AIM:Calcifications of large arteries are frequent in chronic kidney disease (CKD) and may contribute to the high cardiovascular risk in this population. The aim of this study was to examine whether abdominal aortic calcification volume (AACV) was a predictor of the rate of decline in glomerular filtration ra...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000501687
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:In the Chronic REnal Disease in Turkey-CREDIT Study, a large populationbased study on 10,748 adults, the prevalence of chronic kidney disease (CKD) and relationship between CKD and other cardiovascular risk factors had been studied. METHODS:This report presents the results of CREDIT study on the prevalence,...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000339025
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:To evaluate the relationship between FGF23 and changes in biochemical parameters, left ventricle mass index, coronary, aortic and, valve calcifications. METHODS:Totally 185 patients with chronic renal disease were included in this prospective, cross-sectional study. The patients were stratified according to...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000339026
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/AIMS:Serum/Glucocorticoid Regulated Kinase 1 (SGK1) plays a significant role in regulating renal Na(+) reabsorption, K(+) secretion, and blood pressure (BP). This study aimed to assess the association of common genetic variants in the SGK1 gene with BP responses to controlled dietary sodium or potassium inte...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000368518
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Diabetic nephropathy constitutes a large proportion of end-stage kidney failure in diabetic patients. However, the underlying molecular mechanisms remain unclear. METHODS:Db/db diabetic mouse models and high glucose (HG)-induced human renal mesangial cells (HRMCs) were used as research models in vivo and in...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000508078
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND/AIMS:To gain insight into the effect of arginine vasopressin (AVP) on renal hemodynamics in hypertensive rats, we investigated the vasoconstrictive response to AVP on total renal blood flow (RBF) and total and zonal glomerular filtration rate (GFR) in young and old spontaneously hypertensive rats (SHR). A hy...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000054225
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cardiovascular morbidity and mortality are high in patients with chronic kidney disease. We evaluated the influence of small differences in preoperative kidney function on mortality and complications following cardiac surgery. METHODS:This is an observational study that included adult patients undergoi...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000335950
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/AIMS:Kidney function is critical for homocysteine (Hcy) clearance, and plasma Hcy levels are frequently increased in patients with renal failure. Microalbuminuria (MAU) is an important marker of early renal damage caused by hypertension. At present, there is insufficient evidence on the relationship between ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000486013
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND/AIMS:Refugee dialysis is a worldwide growing dilemma with limited experience. This report presents the largest hemodialysis (HD) patient registry data of Syrian refugees in Turkey. METHODS:Demographic, clinical, laboratory, and dialysis practice data of 345 Syrian HD patients during one year were collected ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000498832
更新日期:2019-01-01 00:00:00
abstract::In the Prague hypertensive rat (PHR), a strain of genetic hypertension derived from Wistar, administration of various antihypertensive drugs (AHD) during the developmental phase of hypertension (weeks 5-9 of life) prevents the rise of blood pressure. However, only drugs blocking the renin-angiotensin system (RAS, i.e....
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000025898
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Renal involvement in Takayasu's arteritis (TA) effects the disease outcome and endovascular treatment is an effective treatment of choice. We investigated nitric oxide (NO) levels and the effect of endovascular treatment in renovascular hypertensive TA patients. METHODS:In five hypertensive patients with re...
journal_title:Kidney & blood pressure research
pub_type: 临床试验,杂志文章
doi:10.1159/000063514
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Urine osmolality indicates the ability of the kidney to concentrate the urine and reflects the antidiuretic action of vasopressin. However, results about the association between urine osmolality and adverse renal outcomes in chronic kidney disease (CKD) are conflicting. We investigated the association betwee...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000502291
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:The association between ACYP2(Acylphosphatase 2) polymorphisms and immunoglobulin A nephropathy (IgAN) risk in the Chinese Han population remains unclear. We aimed to evaluate the association between ACYP2 polymorphisms and IgAN risk by performing a case-control study. METHODS:Eleven ACYP2 single nucle...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000501703
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:Cardiorenal syndrome type 1 (CRS1) is a syndrome characterized by a rapid worsening of cardiac function leading to acute kidney injury (AKI). The aims of this study were to investigate the risk factors and the prognosis of CRS1 in elderly patients. METHODS:A total of 312 elderly patients (≥60 years old...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000447936
更新日期:2016-01-01 00:00:00
abstract:AIM:The aim of the present study was to evaluate the influence of cycle exercise during hemodialysis (HD) on patients' physical proficiency, muscle strength, quality of life and selected laboratory parameters. PATIENTS AND METHODS:In a group of 29 (15 female, 14 male) HD patients (age 64.2 ± 13.1 years), 3 months of c...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000335411
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/AIMS:The deposition of β2-microglobulin induced by reactive inflammation causing carpal tunnel syndrome (CTS) is one of the complications of dialysis-related amyloidosis in maintenance hemodialysis (MHD) patients. Air pollution levels, especially particulate matter with an aerodynamic diameter of <2.5 mm (PM...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000484422
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND/AIMS:To prevent contrast induced renal dysfunction a periprocedural prophylactic hydration is applied. Due to dilution it should cause a drop in serum creatinine concentration (SCR). Surprisingly, no reduction in SCR after contrast admission is found in up to 25% of patients as early as 12-18 hours after cor...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000443425
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIMS:Hypertensive patients present with increased oxidative stress and frequently receive angiotensin II (ANGII) receptor type I blockers (ARB) for blood pressure (BP) reduction. Recent studies revealed an important role of ANGII in maintaining vascular oxidative homeostasis, including sustaining normal sodi...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000450562
更新日期:2016-01-01 00:00:00
abstract::The present investigation examined the possible influence of urinary calcium excretion on the concentration of renal calbindin-D28k. Thiazide diuretics stimulate calcium transport across the epithelial cells of the distal tubule, which express calbindin-D28k in high concentrations. Calbindin-D28k is assumed to facilit...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000174153
更新日期:1997-01-01 00:00:00