Seed response to strigolactone is controlled by abscisic acid-independent DNA methylation in the obligate root parasitic plant, Phelipanche ramosa L. Pomel.

Abstract:

:Seed dormancy release of the obligate root parasitic plant, Phelipanche ramosa, requires a minimum 4-day conditioning period followed by stimulation by host-derived germination stimulants, such as strigolactones. Germination is then mediated by germination stimulant-dependent activation of PrCYP707A1, an abscisic acid catabolic gene. The molecular mechanisms occurring during the conditioning period that silence PrCYP707A1 expression and regulate germination stimulant response are almost unknown. Here, global DNA methylation quantification associated with pharmacological approaches and cytosine methylation analysis of the PrCYP707A1 promoter were used to investigate the modulation and possible role of DNA methylation during the conditioning period and in the PrCYP707A1 response to GR24, a synthetic strigolactone analogue. Active global DNA demethylation occurs during the conditioning period and is required for PrCYP707A1 activation by GR24 and for subsequent seed germination. Treatment with 5-azacytidine, a DNA-hypomethylating molecule, reduces the length of the conditioning period. Conversely, hydroxyurea, a hypermethylating agent, inhibits PrCYP707A1 expression and seed germination. Methylated DNA immunoprecipitation followed by PCR experiments and bisulfite sequencing revealed that DNA demethylation particularly impacts a 78-nucleotide sequence in the PrCYP707A1 promoter. The results here demonstrate that the DNA methylation status during the conditioning period plays a crucial role independently of abscisic acid in the regulation of P. ramosa seed germination by controlling the strigolactone-dependent expression of PrCYP707A1.

journal_name

J Exp Bot

authors

Lechat MM,Brun G,Montiel G,Véronési C,Simier P,Thoiron S,Pouvreau JB,Delavault P

doi

10.1093/jxb/erv119

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

3129-40

issue

11

eissn

0022-0957

issn

1460-2431

pii

erv119

journal_volume

66

pub_type

杂志文章
  • Involvement of reactive oxygen species in endosperm cap weakening and embryo elongation growth during lettuce seed germination.

    abstract::Endosperm cap (CAP) weakening and embryo elongation growth are prerequisites for the completion of lettuce seed germination. Although it has been proposed that the cell wall loosening underlying these processes results from an enzymatic mechanism, it is still unclear which enzymes are involved. Here it is shown that r...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru167

    authors: Zhang Y,Chen B,Xu Z,Shi Z,Chen S,Huang X,Chen J,Wang X

    更新日期:2014-07-01 00:00:00

  • Metabolic analysis of kiwifruit (Actinidia deliciosa) berries from extreme genotypes reveals hallmarks for fruit starch metabolism.

    abstract::Tomato, melon, grape, peach, and strawberry primarily accumulate soluble sugars during fruit development. In contrast, kiwifruit (Actinidia Lindl. spp.) and banana store a large amount of starch that is released as soluble sugars only after the fruit has reached maturity. By integrating metabolites measured by gas chr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert293

    authors: Nardozza S,Boldingh HL,Osorio S,Höhne M,Wohlers M,Gleave AP,MacRae EA,Richardson AC,Atkinson RG,Sulpice R,Fernie AR,Clearwater MJ

    更新日期:2013-11-01 00:00:00

  • Multispectral fluorescence and reflectance imaging at the leaf level and its possible applications.

    abstract::Images taken at different spectral bands are increasingly used for characterizing plants and their health status. In contrast to conventional point measurements, imaging detects the distribution and quantity of signals and thus improves the interpretation of fluorescence and reflectance signatures. In multispectral fl...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erl207

    authors: Lenk S,Chaerle L,Pfündel EE,Langsdorf G,Hagenbeek D,Lichtenthaler HK,Van Der Straeten D,Buschmann C

    更新日期:2007-01-01 00:00:00

  • Pine and mistletoes: how to live with a leak in the water flow and storage system?

    abstract::The mistletoe, Viscum album, living on Scots pine (Pinus sylvestris) has been reported barely to regulate its transpiration and thus heavily to affect the gas exchange of its host. The extent of this mistletoe effect and its underlying mechanism has, so far, only been partially analysed. In this study, pine branches w...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err432

    authors: Zweifel R,Bangerter S,Rigling A,Sterck FJ

    更新日期:2012-04-01 00:00:00

  • Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3.

    abstract::Citric acid is the predominant organic acid of citrus fruit. Degradation of citric acid occurs during fruit development, influencing fruit acidity. Associations of CitAco3 transcripts and citric acid degradation have been reported for citrus fruit. Here, transient overexpression of CitAco3 significantly reduced the ci...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erx187

    authors: Li SJ,Yin XR,Wang WL,Liu XF,Zhang B,Chen KS

    更新日期:2017-06-15 00:00:00

  • The role of auxin and gibberellin in tomato fruit set.

    abstract::The initiation of tomato fruit growth, fruit set, is very sensitive to environmental conditions. Therefore, an understanding of the mechanisms that regulate this process can facilitate the production of this agriculturally valuable fruit crop. Over the years, it has been well established that tomato fruit set depends ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erp094

    authors: de Jong M,Mariani C,Vriezen WH

    更新日期:2009-01-01 00:00:00

  • Plants grow with a little help from their organelle friends.

    abstract::Chloroplasts and mitochondria are indispensable for plant development. They not only provide energy and carbon sources to cells, but also have evolved to become major players in a variety of processes such as amino acid metabolism, hormone biosynthesis and cellular signalling. As semi-autonomous organelles, they conta...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erw399

    authors: Van Dingenen J,Blomme J,Gonzalez N,Inzé D

    更新日期:2016-12-01 00:00:00

  • Ethanol breaks dormancy of the potato tuber apical bud.

    abstract::Growing potato tubers or freshly harvested mature tubers have a dormant apical bud. Normally, this dormancy is spontaneously broken after a period of maturation of the tuber, resulting in the growth of a new sprout. Here it is shown that in in vitro-cultured growing and maturing tubers, ethanol can rapidly break this ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri245

    authors: Claassens MM,Verhees J,van der Plas LH,van der Krol AR,Vreugdenhil D

    更新日期:2005-09-01 00:00:00

  • Roles for redox regulation in leaf senescence of pea plants grown on different sources of nitrogen nutrition.

    abstract::Leaf senescence and associated changes in redox components were monitored in commercial pea (Pisum sativum L. cv. Phoenix) plants grown under different nitrogen regimes for 12 weeks until both nodules and leaves had fully senesced. One group of plants was inoculated with Rhizobium leguminosarum and grown with nutrient...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl012

    authors: Vanacker H,Sandalio L,Jiménez A,Palma JM,Corpas FJ,Meseguer V,Gómez M,Sevilla F,Leterrier M,Foyer CH,del Río LA

    更新日期:2006-01-01 00:00:00

  • Identification of predominant genes involved in regulation and execution of senescence-associated nitrogen remobilization in flag leaves of field grown barley.

    abstract::The transcriptomes of senescing flag leaves collected from barley field plots with standard or high nitrogen supply were compared to identify genes specifically associated with nitrogen remobilization during leaf senescence under agronomically relevant conditions. In flag leaves collected in field plots with high nitr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru094

    authors: Hollmann J,Gregersen PL,Krupinska K

    更新日期:2014-07-01 00:00:00

  • Leaf-atmosphere NH(3) exchange of white clover (Trifolium repens L.) in relation to mineral N nutrition and symbiotic N(2) fixation.

    abstract::Plant-atmosphere NH(3) exchange was studied in white clover (Trifolium repens L. cv. Seminole) growing in nutrient solution containing 0 (N(2) based), 0.5 (low N) or 4.5 (high N) mM NO(3)(-). The aim was to show whether the NH(3) exchange potential is influenced by the proportion of N(2) fixation relative to NO(3)(-) ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Herrmann B,Mattsson M,Fuhrer J,Schjoerring JK

    更新日期:2002-01-01 00:00:00

  • Improving the nutritive value of rice seeds: elevation of cysteine and methionine contents in rice plants by ectopic expression of a bacterial serine acetyltransferase.

    abstract::With the aim of increasing the cysteine level in rice (Oryza sativa L.) and thus improving its nutritional quality, transgenic rice plants were generated expressing an Escherichia coli serine acetyltransferase isoform (EcSAT), the enzyme synthesizing O-acetylserine, the precursor of cysteine. The gene was fused to the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers253

    authors: Nguyen HC,Hoefgen R,Hesse H

    更新日期:2012-10-01 00:00:00

  • Integrating modelling and phenotyping approaches to identify and screen complex traits: transpiration efficiency in cereals.

    abstract::Following advances in genetics, genomics, and phenotyping, trait selection in breeding is limited by our ability to understand interactions within the plant and with the environment, and to identify traits of most relevance to the target population of environments. We propose an integrated approach that combines insig...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery059

    authors: Chenu K,Van Oosterom EJ,McLean G,Deifel KS,Fletcher A,Geetika G,Tirfessa A,Mace ES,Jordan DR,Sulman R,Hammer GL

    更新日期:2018-06-06 00:00:00

  • Molecular plant responses to combined abiotic stresses put a spotlight on unknown and abundant genes.

    abstract::Environmental stresses such as drought, heat, and salinity limit plant development and agricultural productivity. While individual stresses have been studied extensively, much less is known about the molecular interaction of responses to multiple stresses. To address this problem, we investigated molecular responses o...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa250

    authors: Sewelam N,Brilhaus D,Bräutigam A,Alseekh S,Fernie AR,Maurino VG

    更新日期:2020-08-06 00:00:00

  • Activation of ACS7 in Arabidopsis affects vascular development and demonstrates a link between ethylene synthesis and cambial activity.

    abstract::Ethylene is a gaseous hormone that affects many processes of plant growth and development. During vascular development, ethylene positively regulates cambial cell division in parallel with tracheary element differentiation inhibitory factor (TDIF) peptide signaling. In this study, we identified an ethylene overproduci...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa423

    authors: Yang S,Wang S,Li S,Du Q,Qi L,Wang W,Chen J,Wang H

    更新日期:2020-12-31 00:00:00

  • Organization of cell walls in Sandersonia aurantiaca floral tissue.

    abstract::Visual symptoms of the onset of senescence in Sandersonia aurantiaca flowers begin with fading of flower colour and wilting of the tissue. When fully senescent, the flowers form a papery shell that remains attached to the plant. The cell walls of these flowers have been examined to determine whether there are wall mod...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.368.513

    authors: O'Donoghue EM,Somerfield SD,Heyes JA

    更新日期:2002-03-01 00:00:00

  • The chemical composition of suberin in apoplastic barriers affects radial hydraulic conductivity differently in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix).

    abstract::Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degradation (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were analysed quantitatively by gas chromatography and mass...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri144

    authors: Schreiber L,Franke R,Hartmann KD,Ranathunge K,Steudle E

    更新日期:2005-05-01 00:00:00

  • From museums to genomics: old herbarium specimens shed light on a C3 to C4 transition.

    abstract::Collections of specimens held by natural history museums are invaluable material for biodiversity inventory and evolutionary studies, with specimens accumulated over 300 years readily available for sampling. Unfortunately, most museum specimens yield low-quality DNA. Recent advances in sequencing technologies, so call...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru395

    authors: Besnard G,Christin PA,Malé PJ,Lhuillier E,Lauzeral C,Coissac E,Vorontsova MS

    更新日期:2014-12-01 00:00:00

  • One-step analysis of seed storage data and the longevity of Arabidopsis thaliana seeds.

    abstract::Seeds of two ecotypes of Arabidopsis thaliana, NW20 and N1601, were aged over a range of saturated salt solutions at temperatures between 6 degrees C and 55 degrees C. For each ecotype, the results from 37 storage experiments were summarized using the Ellis and Roberts viability equations and a modified version of the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg103

    authors: Hay FR,Mead A,Manger K,Wilson FJ

    更新日期:2003-03-01 00:00:00

  • Nod factor-treated Medicago truncatula roots and seeds show an increased number of nodules when inoculated with a limiting population of Sinorhizobium meliloti.

    abstract::Medicago truncatula is a model legume plant that interacts symbiotically with Sinorhizobium meliloti, the alfalfa symbiont. This process involves a molecular dialogue between the bacterium and the plant. Legume roots exude flavonoids that induce the expression of a set of rhizobial genes, the nod genes, which are esse...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh261

    authors: Macchiavelli RE,Brelles-Mariño G

    更新日期:2004-12-01 00:00:00

  • A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

    abstract::Magnesium-chelatase H subunit [CHLH/putative abscisic acid (ABA) receptor ABAR] positively regulates guard cell signalling in response to ABA, but the molecular mechanism remains largely unknown. A member of the sucrose nonfermenting 1 (SNF1)-related protein kinase 2 family, SnRK2.6/open stomata 1 (OST1)/SRK2E, which ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv341

    authors: Liang S,Lu K,Wu Z,Jiang SC,Yu YT,Bi C,Xin Q,Wang XF,Zhang DP

    更新日期:2015-10-01 00:00:00

  • Structure, attachment properties, and ecological importance of the attachment system of English ivy (Hedera helix).

    abstract::Root climbers such as English ivy (Hedera helix) rely on specialized adventitious roots for attachment, enabling the plants to climb on a wide range of natural and artificial substrates. Despite their importance for the climbing habit, the biomechanical properties of these specialized adventitious roots compared with ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err260

    authors: Melzer B,Seidel R,Steinbrecher T,Speck T

    更新日期:2012-01-01 00:00:00

  • Down-regulation of a LBD-like gene, OsIG1, leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice.

    abstract::The indeterminate gametophyte1 (ig1) mutation was first characterized to modulate female gametophyte development in maize (Zea mays). However, the function of its rice orthologue, OsIG1, remains unknown. For this, we first analysed OsIG1 localization from differential tissues in rice. Real-time quantitative PCR (qRT-P...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru396

    authors: Zhang J,Tang W,Huang Y,Niu X,Zhao Y,Han Y,Liu Y

    更新日期:2015-01-01 00:00:00

  • Calcium channel CNGC19 mediates basal defense signaling to regulate colonization by Piriformospora indica in Arabidopsis roots.

    abstract::The activation of calcium signaling is a crucial event for perceiving environmental stress. Colonization by Piriformospora indica, a growth-promoting root endosymbiont, activates cytosolic Ca2+ in Arabidopsis roots. In this study, we examined the role and functional relevance of calcium channels responsible for Ca2+ f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa028

    authors: Jogawat A,Meena MK,Kundu A,Varma M,Vadassery J

    更新日期:2020-05-09 00:00:00

  • A multi-model framework for the Arabidopsis life cycle.

    abstract::Linking our understanding of biological processes at different scales is a major conceptual challenge in biology and aggravated by differences in research methods. Modelling can be a useful approach to consolidating our understanding across traditional research domains. The laboratory model species Arabidopsis is very...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery394

    authors: Zardilis A,Hume A,Millar AJ

    更新日期:2019-04-29 00:00:00

  • Early changes in membrane permeability, production of oxidative burst and modification of PAL activity induced by ergosterol in cotyledons of Mimosa pudica.

    abstract::Ergosterol (a fungal membrane component) was shown to induce transient influx of protons and membrane hyperpolarization in cotyledonary cells of Mimosa pudica L. By contrast, chitosan (a fungal wall component with known elicitor properties) triggered membrane depolarization. In the processes induced by ergosterol, a s...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erj090

    authors: Rossard S,Luini E,Pérault JM,Bonmort J,Roblin G

    更新日期:2006-01-01 00:00:00

  • High temperature stress of Brassica napus during flowering reduces micro- and megagametophyte fertility, induces fruit abortion, and disrupts seed production.

    abstract::High temperature stress (HTS), during flowering, decreases seed production in many plants. To determine the effect of a moderate HTS on flowering, fruit and seed set in Brassica napus, plants were exposed to a HTS (8/16 h dark/light, 18 degrees C night, ramped at 2 degrees C h-1, over 6 h, to 35 degrees C for 4 h, ram...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erh038

    authors: Young LW,Wilen RW,Bonham-Smith PC

    更新日期:2004-02-01 00:00:00

  • Variation in the shoot calcium content of angiosperms.

    abstract::This study describes the variation in the mean relative shoot Ca content within the angiosperms at the ordinal level. Data were derived from studies in the literature in which the shoot Ca content of two or more species had been compared, and from a hydroponic experiment in which plants were selected to represent the ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erg143

    authors: Broadley MR,Bowen HC,Cotterill HL,Hammond JP,Meacham MC,Mead A,White PJ

    更新日期:2003-05-01 00:00:00

  • A network perspective on nitrogen metabolism from model to crop plants using integrated 'omics' approaches.

    abstract::Nitrogen (N), as an essential element in amino acids, nucleotides, and proteins, is a key factor in plant growth and development. Omics approaches such as metabolomics and transcriptomics have become a promising way to inspect complex network interactions in N metabolism and can be used for monitoring the uptake and r...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/eru322

    authors: Fukushima A,Kusano M

    更新日期:2014-10-01 00:00:00

  • Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant.

    abstract::The Arabidopsis thaliana ascorbate-deficient vtc-1 mutant has only 30% ascorbate contents of the wild type (WT). This ascorbate-deficient mutant was used here to study the physiological roles of ascorbate under salt stress in vivo. Salt stress resulted in a more significant decrease in CO2 assimilatory capacity in the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eri301

    authors: Huang C,He W,Guo J,Chang X,Su P,Zhang L

    更新日期:2005-12-01 00:00:00