Abstract:
:Clustered DNA damage is a specific type of DNA damage induced by ionizing radiation. Any type of ionizing radiation traverses the target DNA molecule as a beam, inducing damage along its track. Our previous study showed that clustered DNA damage yields decreased with increased linear energy transfer (LET), leading us to investigate the importance of clustered DNA damage in the biological effects of heavy ion beam radiation. In this study, we analyzed the yield of clustered base damage (comprising multiple base lesions) in cultured cells irradiated with various heavy ion beams, and investigated isolated base damage and the repair process in post-irradiation cultured cells. Chinese hamster ovary (CHO) cells were irradiated by carbon, silicon, argon and iron ion beams with LETs of 13, 55, 90 and 200 keV µm(-1), respectively. Agarose gel electrophoresis of the cells with enzymatic treatments indicated that clustered base damage yields decreased as the LET increased. The aldehyde reactive probe procedure showed that isolated base damage yields in the irradiated cells followed the same pattern. To analyze the cellular base damage process, clustered DNA damage repair was investigated using DNA repair mutant cells. DNA double-strand breaks accumulated in CHO mutant cells lacking Xrcc1 after irradiation, and the cell viability decreased. On the other hand, mouse embryonic fibroblast (Mef) cells lacking both Nth1 and Ogg1 became more resistant than the wild type Mef. Thus, clustered base damage seems to be involved in the expression of heavy ion beam biological effects via the repair process.
journal_name
J Radiat Resjournal_title
Journal of radiation researchauthors
Tokuyama Y,Furusawa Y,Ide H,Yasui A,Terato Hdoi
10.1093/jrr/rru122subject
Has Abstractpub_date
2015-05-01 00:00:00pages
446-55issue
3eissn
0449-3060issn
1349-9157pii
rru122journal_volume
56pub_type
杂志文章abstract::The effectiveness of an Electronic Personal Dosemeter (Siemens EPD) for cosmic-radiation dosimetry at aviation altitudes was examined on eight international flights between March and September, 1998. The EPD values (Hepd) of the dose equivalent from penetrating radiation, Hp(10), were assumed to be almost the same as ...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.42.57
更新日期:2001-03-01 00:00:00
abstract::Intrafractional motion and interfractional changes affect the accuracy of the delivered dose in radiotherapy, particularly in charged-particle radiotherapy. Most recent studies are focused on intrafractional motion (respiratory motion). Here, we report a quantitative simulation analysis of the effects of interfraction...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.09032
更新日期:2009-11-01 00:00:00
abstract::In Japan, the use of adjuvant radiotherapy after prostatectomy for prostate cancer has not increased compared with the use of salvage radiotherapy. We retrospectively evaluated the outcome of adjuvant radiotherapy together with prognostic factors of outcome in Japan. Between 2005 and 2007, a total of 87 patients were ...
journal_title:Journal of radiation research
pub_type: 杂志文章,多中心研究
doi:10.1093/jrr/rrt137
更新日期:2014-05-01 00:00:00
abstract::Massive release of radioactive materials into the atmosphere occurred due to the Fukushima Daiichi Nuclear Power Station (FDNPS) accident in March 2011. The World Health Organization (WHO) and the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR) reported the results of dose estimation t...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrz089
更新日期:2020-03-23 00:00:00
abstract::Disufiram (a drug used for the treatment of alcoholism) protected microsomal membranes and plasmid DNA against damages induced by gamma-radiation. The peroxidation of membrane lipids increased linearly with the radiation dose up to 600 Gy, and the presence of disulfiram inhibited membrane lipid peroxidation as assayed...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.44.255
更新日期:2003-09-01 00:00:00
abstract::This study aimed to identify factors that predict prognosis after radiotherapy for brain metastases (BMs) from small-cell lung cancer (SCLC). This study retrospectively evaluated 48 consecutive patients who underwent whole-brain radiotherapy (WBRT) for BMs from SCLC between February 2008 and December 2017. WBRT was de...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rry107
更新日期:2019-03-01 00:00:00
abstract::To evaluate the effect of the sympathetic nervous system on radiation-induced apoptosis in jejunal crypt cells, apoptosis levels were compared in spontaneously hypertensive rats (SHR), animals which are a genetic hyperfunction model of the sympathetic nervous system, and normotensive Wistar-Kyoto rats (WKY). SHR and W...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.41.55
更新日期:2000-03-01 00:00:00
abstract::We have proposed four dimensional (4D) digitally reconstructed radiography (DRR) for verifying a lung tumor position during volumetric modulated arc therapy (VMAT). An internal target volume (ITV) was defined based on two clinical target volumes (CTVs) delineated on maximum exhalation and maximum inhalation images acq...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrs013
更新日期:2012-07-01 00:00:00
abstract::We evaluated toxicity and outcomes for patients with peripheral lung tumors treated with stereotactic body radiation therapy (SBRT) in a dose-escalation and dose-convergence study. A total of 15 patients were enrolled. SBRT was performed with 60 Gy in 5 fractions (fr.) prescribed to the 60% isodose line of maximum dos...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rru037
更新日期:2014-09-01 00:00:00
abstract::The effect of gamma-irradiation on a major nutritional function of the yolk sac (the uptake and digestion of macromolecular materials) was studied in rat visceral yolk sacs cultured in vitro being used 125I-labeled bovine serum albumin (RISA) as the tracer protein. The uptake of RISA (per g wet weight) by rat yolk sac...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.34.171
更新日期:1993-06-01 00:00:00
abstract::A synergistic interaction of hyperthermia with ionizing or UV light (254 nm) radiation was analyzed in experiments with yeast cells. For a fixed dose rate of radiation, the synergism was shown to be observed only within a definite temperature range (40-45 degrees C), inside of which there was an optimal temperature to...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.42.361
更新日期:2001-12-01 00:00:00
abstract::In hypofractionated stereotactic radiotherapy (SRT), high doses per fraction are usually used and the dose delivery pattern is different from that of conventional radiation. The daily dose is usually given intermittently over a longer time compared with conventional radiotherapy. During prolonged radiation delivery, s...
journal_title:Journal of radiation research
pub_type: 杂志文章,评审
doi:10.1093/jrr/rrw015
更新日期:2016-08-01 00:00:00
abstract::The estimation of the dose and the irradiated fraction of the body is important information in the primary medical response in case of a radiological accident. The PCC-R assay has been developed for high-dose estimations, but little attention has been given to its applicability for partial-body irradiations. In the pr...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrt032
更新日期:2013-09-01 00:00:00
abstract::We examined the use of the mouse Pc-3 ESTR (expanded simple tandem repeat) locus and 72 human microsatellite loci as potentially sensitive biomarkers for mutagenic exposures to germ cells in mice and humans respectively. In the mouse work, we treated male mice with TCDD (2, 3, 7, 8-tetrachlorodibenzo-p-dioxin; a chemi...
journal_title:Journal of radiation research
pub_type: 历史文章,杂志文章
doi:10.1269/jrr.47.b31
更新日期:2006-01-01 00:00:00
abstract::To assess the mutational effects of radiation on vigorously proliferating plant tissue, the mutation spectrum was analyzed with Arabidopsis seedlings using the plasmid-rescue method. Transgenic plants containing the Escherichia coli rpsL gene were irradiated with γ-rays and carbon ion beams (320-MeV (12)C(6+)), and mu...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrt074
更新日期:2013-11-01 00:00:00
abstract::The aim of this study is to evaluate the potential radioprotective effects of N-acetylcysteine (NAC) against genotoxicity and cytotoxicity. The effect of WR-2721, as a representative of clinically used radioprotector, was compared with that of NAC, using the chromosomal aberration (CA) and micronucleus (MN) test syste...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.08066
更新日期:2009-01-01 00:00:00
abstract::This study aimed to clarify the motivations and timing of the decision to become radiation oncologists. MATERIALS AND METHODS:We conducted an online survey for new members of the Japanese Society for Radiation Oncology (JASTRO). RESULTS:The response rate was 43.3%. Data of the 79 respondents who wanted to obtain a b...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rraa051
更新日期:2020-09-08 00:00:00
abstract::This project aimed to set up a Beagle dog model of radiation-induced lung injury in order to supply fresh lung tissue samples in the different injury phases for gene and protein research. Three dogs received 18 Gy X-ray irradiation in one fraction, another three dogs received 8 Gy in each of three fractions at weekly ...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrw053
更新日期:2016-09-01 00:00:00
abstract::The study aimed to detect features of human serum proteome that were associated with exposure to ionizing radiation. The analyzed group consisted of 46 patients treated with radical radiotherapy for larynx cancer; patients were irradiated with total doses in a range from 51 to 72 Gy. Three consecutive blood samples we...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.11019
更新日期:2011-01-01 00:00:00
abstract::Resveratrol has received considerable attention as a polyphenol with anti-oxidant, anti-carcinogenic, and anti-inflammatory effects. Radiation is an important component of therapy for a wide range of malignant conditions. However, it causes damage to normal cells and, hence, can result in adverse side effects. This st...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrt140
更新日期:2014-05-01 00:00:00
abstract::Estimation of the relative biological effectiveness (RBE) of the proton beam at the National Cancer Center Proton Therapy Center in Korea (NCCPTC) is required clinically for the treatment of cancer. The proton beam was fixed at 190 MeV with 6 cm a spread out Bragg peaks (SOBP) for which corresponds to most frequent cl...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.08017
更新日期:2008-09-01 00:00:00
abstract::Low doses (2.5-15 cGy) of X-irradiation two months prior to a second exposure to a sublethal dose enhanced the survival rate in mice. Optimal and significant increase was observed with 5-10 cGy. Endogenous spleen colony counts (endo-CFUs) after 7 Gy increased by a factor of about 1.7 in mice pre-irradiated with 5-10 c...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.31.256
更新日期:1990-09-01 00:00:00
abstract::Various kinases, such as tyrosine, protein kinase C (PKC) and MAP kinase, play important role in the cellular response to radiation, but little is known about the specific response in the whole animal. Most studies, except a few, are based on single cells. There is a paucity of data where signaling following whole bod...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.45.127
更新日期:2004-03-01 00:00:00
abstract::The purpose of this study is to survey the current patterns of practice, and barriers to implementation, of 3D image-guided brachytherapy (3D-IGBT) for cervical cancer in Japan. A 30-item questionnaire was sent to 171 Japanese facilities where high-dose-rate brachytherapy devices were available in 2012. In total, 135 ...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrv047
更新日期:2015-11-01 00:00:00
abstract::We examined the effect of an extremely low-frequency magnetic field (ELFMF) at 5, 50 and 400 mT on DNA strand breaks in human glioma MO54 cells. A DNA damage analysis was performed using the method of alkaline comet assay. The cells were exposed to X-rays alone (5 Gy), ELFMF alone, or X-rays followed by ELFMF at 4 deg...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.41.293
更新日期:2000-09-01 00:00:00
abstract::We aimed to investigate the effect of chronic radiation exposure associated with the Fukushima Daiichi Nuclear Power Plant (FNPP) accident on the testes of boar and inobuta (a hybrid of Sus scrofa and Sus scrofa domestica). This study examined the contamination levels of radioactive caesium (Cs), especially (134)Cs an...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rrv070
更新日期:2015-12-01 00:00:00
abstract::Respiratory motion management is a huge challenge in radiation therapy. Respiratory motion induces temporal anatomic changes that distort the tumor volume and its position. In this study, a markerless tumor-tracking algorithm was investigated by performing phase recognition during stereotactic body radiation therapy (...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rry085
更新日期:2019-01-01 00:00:00
abstract::The expression of oncogenes was studied in 12 types of 178 mouse tumors induced by radiations and chemicals. DNA was analyzed in tumors in which the overexpression of oncogenes was noted. Amplification of the myc oncogene was found in chemically induced sarcomas, but not in sarcomas induced by radiation. Activation of...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.32.supplement2_235
更新日期:1991-12-01 00:00:00
abstract::The purpose of this study was to assess the toxicity and efficacy of multimodal approaches, including three-dimensional conformal re-irradiation, for patients with recurrent or persistent esophageal cancer after radiotherapy. Thirty-one patients with esophageal cancer treated with three-dimensional conformal re-irradi...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1269/jrr.11066
更新日期:2011-01-01 00:00:00
abstract::Radiation-induced rescue effect (RIRE) in cells refers to the phenomenon where irradiated cells (IRCs) receive help from feedback signals produced by partnered bystander unirradiated cells (UIRCs) or from the conditioned medium (CM) that has previously conditioned the UIRCs. In the present work, we explored the role o...
journal_title:Journal of radiation research
pub_type: 杂志文章
doi:10.1093/jrr/rraa023
更新日期:2020-05-22 00:00:00