Abstract:
:All known mechanisms of mitotic spindle orientation rely on astral microtubules. We report that even in the absence of astral microtubules, metaphase spindles in MDCK and HeLa cells are not randomly positioned along their x-z dimension, but preferentially adopt shallow β angles between spindle pole axis and substratum. The nonrandom spindle positioning is due to constraints imposed by the cell cortex in flat cells that drive spindles that are longer and/or wider than the cell's height into a tilted, quasidiagonal x-z position. In rounder cells, which are taller, fewer cortical constraints make the x-z spindle position more random. Reestablishment of astral microtubule-mediated forces align the spindle poles with cortical cues parallel to the substratum in all cells. However, in flat cells, they frequently cause spindle deformations. Similar deformations are apparent when confined spindles rotate from tilted to parallel positions while MDCK cells progress from prometaphase to metaphase. The spindle disruptions cause the engagement of the spindle assembly checkpoint. We propose that cell rounding serves to maintain spindle integrity during its positioning.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Lázaro-Diéguez F,Ispolatov I,Müsch Adoi
10.1091/mbc.E14-08-1330subject
Has Abstractpub_date
2015-04-01 00:00:00pages
1286-95issue
7eissn
1059-1524issn
1939-4586pii
mbc.E14-08-1330journal_volume
26pub_type
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