Global DNA methylation in neonatal sepsis.

Abstract:

OBJECTIVE:To find out whether gDNA methylation can be used as a diagnostic/prognostic method for neonatal sepsis. METHODS:The study was conducted in the neonatal division of a tertiary care referral hospital. Fifty one newborns as cases and thirty seven newborns as controls were enrolled in the study. Using 5-mC DNA ELISA method, the percentage of genomic DNA methylated in these newborns was established. RESULTS:Highly significant difference in percentage of gDNA methylated was found between the cases and controls (Cases: 2.4 ± 0.39; CONTROLS:2.07 ± 0.35; P < 0.0001). Culture proven and possible cases were also significantly distinguishable (P < 0.05). No significant differences in methylation were observed in terms of gestational age, birth weight and outcomes such shock, thrombocytopenia, except for renal failure. CONCLUSIONS:The index results showed that genomic DNA methylation varies significantly among newborns with sepsis (clinical, probable and culture positive) and without sepsis. Although the global DNA methylation was not a highly sensitive diagnostic method, this study reveals that DNA methylation might play a vital role in neonatal sepsis susceptibility. Identification of the specific differentially methylated genes might serve as a promising future diagnostic/prognostic marker for neonatal sepsis.

journal_name

Indian J Pediatr

authors

Dhas BB,Antony HA,Bhat V,Newton B,Parija SC

doi

10.1007/s12098-014-1574-5

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

340-4

issue

4

eissn

0019-5456

issn

0973-7693

journal_volume

82

pub_type

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