Abstract:
OBJECTIVE:To find out whether gDNA methylation can be used as a diagnostic/prognostic method for neonatal sepsis. METHODS:The study was conducted in the neonatal division of a tertiary care referral hospital. Fifty one newborns as cases and thirty seven newborns as controls were enrolled in the study. Using 5-mC DNA ELISA method, the percentage of genomic DNA methylated in these newborns was established. RESULTS:Highly significant difference in percentage of gDNA methylated was found between the cases and controls (Cases: 2.4 ± 0.39; CONTROLS:2.07 ± 0.35; P < 0.0001). Culture proven and possible cases were also significantly distinguishable (P < 0.05). No significant differences in methylation were observed in terms of gestational age, birth weight and outcomes such shock, thrombocytopenia, except for renal failure. CONCLUSIONS:The index results showed that genomic DNA methylation varies significantly among newborns with sepsis (clinical, probable and culture positive) and without sepsis. Although the global DNA methylation was not a highly sensitive diagnostic method, this study reveals that DNA methylation might play a vital role in neonatal sepsis susceptibility. Identification of the specific differentially methylated genes might serve as a promising future diagnostic/prognostic marker for neonatal sepsis.
journal_name
Indian J Pediatrjournal_title
Indian journal of pediatricsauthors
Dhas BB,Antony HA,Bhat V,Newton B,Parija SCdoi
10.1007/s12098-014-1574-5subject
Has Abstractpub_date
2015-04-01 00:00:00pages
340-4issue
4eissn
0019-5456issn
0973-7693journal_volume
82pub_type
杂志文章abstract::This study was conducted in children under the age of four years for a period of one year. By domiciliary visits the diarrheal episodes were recorded, analysed and presented. The findings are based on the diarrheal experience of 4860 children. The attack rate was 2.6/child/year. Family with more than one child had mor...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02751121
更新日期:1991-03-01 00:00:00
abstract::A 15-year-old boy presented to the emergency unit complaining of dyspnea, urticaria and vomiting developed after he fell down when he was playing football. Abdominal ultrasound showed a ruptured hydatid cyst in the right lobe of the liver which was of communicating type. Echinococcus granulosus serologic tests were po...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-009-0011-7
更新日期:2009-03-01 00:00:00
abstract::Cerebral palsy is a common neurodevelopmental condition encountered by pediatricians. The condition may present itself in many different clinical spectra. The etiological and risk factors are many and an awareness of the interplay of multiple factors in the causation of CP is crucial. In many cases, the cause of Cereb...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/BF02731117
更新日期:2005-10-01 00:00:00
abstract::Thirty patients below the age of 15 years (range 8-1/2 to 15 years, mean 11.8 years) have been studied for the severity of rheumatic mitral regurgitation. Moderately severe to severe pulmonary venous hypertension was found in 76.6% and pulmonary arterial hypertension in 60%. Left ventricular volumes could be calculate...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02832994
更新日期:1992-09-01 00:00:00
abstract:OBJECTIVE:A) To study the frequency of perceived breast milk insufficiency in mothers of hospitalized neonates. b) To assess subsequent duration of exclusive breast feeding and growth (upto three months) in neonates whose mothers perceived breast milk insufficiency. METHODS:Neonates whose mothers were willing to breas...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-009-0204-0
更新日期:2009-10-01 00:00:00
abstract::Effective communication with parents is a very important skill for pediatricians especially in a neonatal setup. The authors analyzed non-verbal communication of medical caregivers during counseling sessions. Recorded videos of counseling sessions from the months of March-April 2016 were audited. Counseling episodes w...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-018-2680-6
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:To devise some indicators for measuring and evaluating the neonatal cardiac reserve by using phonocardiogram test (PCGT). METHODS:Two hundred and thirty one (231) full term and one hundred and fifty four (154) preterm infants participated in this study. The amplitude ratio of the first heart sound to the sec...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-010-0058-5
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVES:Management of neuroblastoma, especially high-risk (HR) disease is difficult in a resource-limited setting. There is a paucity of literature on outcomes of patients treated in India. The present study was conducted to analyse the clinical profile, treatment, and outcomes of patients with neuroblastoma treated...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-018-2834-6
更新日期:2019-05-01 00:00:00
abstract::The post eighteen months have been exciting time for craniosynostosis research. In a rapid flurry of publications, mutations of fibroblast growth factor receptors (FGFRs) have been identified in three of the best known craniosynostosis syndromes, namely Apert, Crouzon and Pfeiffer syndromes, as well as in Jackson-Weis...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/BF02751527
更新日期:1996-05-01 00:00:00
abstract:OBJECTIVE:To evaluate the outcome and prognostic factors for oncology patients in the PICU of a tertiary care centre in a developing country. METHODS:A retrospective chart review was done to assess the outcome of children with cancer in the pediatric intensive care unit (PICU) of a developing country from January 2000...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-011-0391-3
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVE:To demonstrate the effectiveness of the hour-specific serum bilirubin nomogram, described by Bhutani et al, in predicting significant hyperbilirubinemia in term Turkish newborns. METHODS:A total of 217 healthy term newborns without signs of hemolysis were enrolled. Serum bilirubin levels were obtained at dis...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-008-0248-6
更新日期:2008-05-01 00:00:00
abstract::Gastrointestinal tumours are rare in children and leiomyoblastoma of small intestine is still rarer. We report a case of leiomyoblastoma of small intestine in a child who presented with acute intestinal obstruction. ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02831363
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVE:Of this pilot study was to assess the iron status and dietary intake of 1-3 year-old apparently healthy toddlers of the lower socio-economic class, and the effect of eight weeks intervention with liquid oral iron in an urban slum in Pune, India. METHODS:50 toddlers (M= 25, F= 25) with mean age of 2.4 years (...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-008-0038-1
更新日期:2008-04-01 00:00:00
abstract::A two-and-a-half-year-old male child presented with recurrent attacks of intractable vomiting, psychomotor retardation since 14 months of age. He had also lower cranial nerve palsy and corticospinal involvement. Magnetic resonance imaging had shown multiple well circumscribed areas of hypointusity in T1 weighted image...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02730822
更新日期:1996-09-01 00:00:00
abstract::Conducting economic evaluation alongside clinical trials is an efficient way of getting valid and reliable information with minimum assumptions made during data collection. However, design of a randomised control trial (RCT) takes away several of the real world situations, the variations in resource utilisation, their...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02820689
更新日期:2000-05-01 00:00:00
abstract::A confirmed case of cholera in a 3-day-old neonate is being reported. Possible source of infection could be by holy water (Chamamrit) given to the baby, which is common ritual in India. Mother's milk has never been reported to transmit cholera, even though mother herself may be suffering from cholera. Contaminated wat...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02752426
更新日期:2001-08-01 00:00:00
abstract::A fifteen-month-old boy, born to consanguineously married couple, presented with asymptomatic hepatomegaly. Investigations revealed mildly deranged liver functions, necroinflammatory changes and cirrhosis on liver biopsy, a markedly raised alpha feto protein and tyrosine levels in plasma and a generalized aminoaciduri...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02820214
更新日期:2006-02-01 00:00:00
abstract::Various blood indices vary in a newborn as compared to older child or adult. It depends on the gestational age, day of life, maternal factors, mode of delivery and site of blood collection. Hemoglobin, HCT & MCV tend to be higher in newborns. They further increase in first 2 days of life. Reticulocytosis and presence ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/BF02731037
更新日期:1998-09-01 00:00:00
abstract::We present a 10-year-old girl who presented to our emergency services with difficulty in breathing of 2-days duration and progressive weakness of a month's duration. In a previous admission elsewhere, she had not been detected to have hyperthyroidism or electrolyte abnormalities. On admission, the child was in hyperca...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-007-0194-8
更新日期:2007-11-01 00:00:00
abstract:OBJECTIVE:An antibiotic-sensitivity study was conducted on 117 strains of group A streptococcus (GAS), 60 strains of group G streptococcus (GGS) and 20 strains of group C streptococcus (GCS) in order to understand current resistance trends. METHODS:All strains were tested by the Kirby Bauer disc diffusion method to 15...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-007-0200-1
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVE:To examine the effect of parental training on disturbed behavior of early childhood cases presented to the pediatricians. METHODS:The patients who reported in pediatric OPD of the Himalayan Institute of Medical Sciences, Dehradun, with complaints of low learning, poor memory, vertigo, speech problem, stress,...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-011-0401-5
更新日期:2011-08-01 00:00:00
abstract::Intrauterine growth retardation (IUGR) is an important determinant of neonatal mortality, morbidity and poor neurologic outcome. The study was aimed to evaluate the magnitude of perinatal risk factors in causation and the neonatal outcome of small for gestational age (SGA) babies. One hundred and three SGA babies born...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02752452
更新日期:1997-03-01 00:00:00
abstract:OBJECTIVES:To identify biomarkers for neuronal injury and outcome in perinatal asphyxia. METHODS:This prospective cohort study was done in authors' level III NICU involving 80 neonates - 40 babies with perinatal asphyxia and 40 weight and gender matched normal neonates. Levels of cytokines IL-6, IL -1β, IL-2 and TNF -...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-014-1575-4
更新日期:2015-05-01 00:00:00
abstract::The original Jones Criteria as proposed by Dr. T. Duckett Jones have been modified four times and the updated revised criteria were published in 1992. According to this latest publication major manifestations are carditis, polyarthritis, chorea, erythema marginatum and subcutaneous nodules. Minor manifestations includ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/BF02758174
更新日期:2000-04-01 00:00:00
abstract::Functional follicular ovarian cysts are frequently reported in girls with peripheral precocious puberty (PP). These cysts are usually self-limiting and resolve spontaneously. Several drugs like antiestrogens (tamoxifen) and new aromatase inhibitors are seldom used for treatment. Here we report a girl with peripheral P...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-009-0190-2
更新日期:2009-09-01 00:00:00
abstract::Allgrove syndrome consists of triad of Adrenal hypoplasia, Achalasia cardia and Alacrimia. Often the first manifestation of adrenal hypoplasia is Addisonian crisis precipitated by infection or trauma. We present case of a 9-year-old boy who presented in shock due to adrenal failure later confirmed to have Allgove synd...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-007-0179-7
更新日期:2007-10-01 00:00:00
abstract::A study on twin births was conducted from May 1993 to April 1994 at S.A.T. Hospital, Thiruvananthapuram. The twinning rate in this period was found as 17.33 per 1000 births. 79.6% babies in the twin pairs were of like sex and 20.4% were of unlike sex. The incidence of low birth weight twin babies in this study was 68....
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02753602
更新日期:1995-05-01 00:00:00
abstract::Fainting attack or syncope in children is a common occurrence, with vasovagal syncope being the commonest cause for majority of pediatric syncope. The aim of emergency room evaluation is not to miss the rare serious underlying disorder causing syncope. A complete detailed history of the event followed by physical exam...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-011-0497-7
更新日期:2012-03-01 00:00:00
abstract::Laboratory investigations are an integral part of evaluation of a child with rheumatic disease. In rheumatic diseases markers of inflammation like CRP and erythrocyte sedimentation are usually raised. In addition, various autoantibodies like anti-nuclear antibody, rheumatoid factor, anti-neutrophil cytoplasmic antibod...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02723714
更新日期:2002-10-01 00:00:00
abstract::The clinical presentation of pseudohypoaldosteronism (PHA) mimics congenital adrenal hyperplasia (CAH). Poor response of the dehydration and electrolyte abnormalities to steroid therapy should make one suspect PHA. The treatment is supportive in the form of salt replacement and sodium resonium. We report a case of PHA...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02758269
更新日期:2006-01-01 00:00:00