Abstract:
:The fish Vitellogenin (Vg) gene has been applied as a biomarker for exposure to estrogenic compounds in the aquatic environment. In this study, we cloned and characterized Vg cDNA from the Korean rose bitterling Rhodeus uyekii (Ru-Vg). The Ru-Vg cDNA encodes a 1424-amino-acid polypeptide that belongs to the VgAo1 family and contains a putative signal peptide, lipovitellin I, phosvitin, and lipovitellin II, but does not contain the vWFD domain or the C-terminal peptide. The deduced Ru-Vg protein has high amino acid identity (73.97%-32.17%) with fish Vg proteins. Pairwise alignment and phylogenetic analysis revealed that Ru-Vg is most closely related to Acheilognathus yamatsutae Vg. Ru-Vg transcripts were detected using quantitative polymerase chain reaction in all tissues tested, with the highest level of expression observed in the ovary. Ru-Vg mRNA was upregulated in R. uyekii hepatopancreas cells in response to treatment with 17β-estradiol (E2) or 17α-ethinylestradiol (EE2). Luciferase reporter expression, driven by the 5'-regulatory region of the Ru-Vg gene spanning from -1020 bp to the start codon was induced by the estrogen receptor and was synergistically activated by treatment with E2 or EE2. These results suggest that R. uyekii and the Ru-Vg gene may be useful as biomarkers for exposure to E2 or EE2.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kong HJ,Kim JL,Moon JY,Kim WJ,Kim HS,Park JY,Cho HK,An CMdoi
10.3390/ijms151018804subject
Has Abstractpub_date
2014-10-17 00:00:00pages
18804-18issue
10issn
1422-0067pii
ijms151018804journal_volume
15pub_type
杂志文章abstract::Phosphodiesterases (PDEs) are essential enzymes for the regulation of pathways mediated by cyclic adenosine monophosphate (cAMP). Secondary plant compounds like anthocyanins (ACs) can inhibit PDE activity and, consequently, may be beneficial for lipid metabolism. This study investigated 18 AC-rich juice extracts and p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21186934
更新日期:2020-09-21 00:00:00
abstract::The mitogen-activated protein kinase-activated protein kinase MK5 is a substrate of the mitogen-activated protein kinases p38, ERK3 and ERK4. Cell culture and animal studies have demonstrated that MK5 is involved in tumour suppression and promotion, embryogenesis, anxiety, cell motility and cell cycle regulation. In t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15034878
更新日期:2014-03-19 00:00:00
abstract::Adolescence represents a vulnerable period for the psychiatric consequences of delta9-tetrahydrocannabinol (Δ⁸-THC) exposure, however, the molecular underpinnings of this vulnerability remain to be established. Histone modifications are emerging as important epigenetic mechanisms involved in the etiopathogenesis of ps...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18102094
更新日期:2017-10-04 00:00:00
abstract::This study was performed to elucidate the molecular function of the synoviocyte proliferation-associated in collagen-induced arthritis (CIA) 1/serum amyloid A-like 1 (SPACIA1/SAAL1) in mice CIA, an animal model of rheumatoid arthritis (RA), and human RA-synovial fibroblasts (RASFs). SPACIA1/SAAL1-deficient mice were g...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123828
更新日期:2018-11-30 00:00:00
abstract::Abnormal filamentous aggregates that are formed by tangled tau protein turn out to be classic amyloid fibrils, meeting all the criteria defined under the fuzzy oil drop model in the context of amyloid characterization. The model recognizes amyloids as linear structures where local hydrophobicity minima and maxima prop...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19102910
更新日期:2018-09-25 00:00:00
abstract::In this study, drug nanocarriers were designed using linear copolymers with different contents of cholinium-based ionic liquid units, i.e., [2-(methacryloyloxy)ethyl]trimethylammonium chloride (TMAMA/Cl: 25, 50, and 75 mol%). The amphiphilicity of the copolymers was evaluated on the basis of their critical micelle con...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010284
更新日期:2020-12-30 00:00:00
abstract::Grass carp (Ctenopharyngodon idellus) is an important worldwide commercial freshwater culture species. However, grass carp reovirus (GCRV) causes serious hemorrhagic disease in fingerlings and yearlings of fishes. To understand the molecular pathogenesis of host cells during GCRV infection, intensive proteomic quantif...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112419
更新日期:2017-11-14 00:00:00
abstract::Besides being traditionally used to relieve hepatobiliary disorders, Cynara cardunculus L. has evidenced anticancer potential on triple-negative breast cancer (TNBC). This study highlights the antiproliferative effects of lipophilic extracts from C. cardunculus L. var. altilis (DC) leaves and florets, and of their maj...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18010063
更新日期:2016-12-29 00:00:00
abstract::Synthetic organic dyes are widely used in various industrial sectors but are also among the most harmful water pollutants. In the last decade, significant efforts have been made to develop improved materials for the removal of dyes from water, in particular, on nanostructured adsorbent materials. Metal organic framewo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020788
更新日期:2021-01-14 00:00:00
abstract::NW-G01, produced by Streptomyces alboflavus 313, is a novel cyclic hexapeptide antibiotic with many potential applications, including antimicrobial activity and antitumor agents. This study developed a system for optimizing medium components in order to enhance NW-G01 production. In this study, Plackett-Burman design ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13045230
更新日期:2012-01-01 00:00:00
abstract::Impaired fracture healing in aged females is still a challenge in clinics. MicroRNAs (miRNAs) play important roles in fracture healing. This study aims to identify the miRNAs that potentially contribute to the impaired fracture healing in aged females. Transverse femoral shaft fractures were created in adult and aged ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17081260
更新日期:2016-08-03 00:00:00
abstract::The variety of metagenomes in current databases provides a rapidly growing source of information for comparative studies. However, the quantity and quality of supplementary metadata is still lagging behind. It is therefore important to be able to identify related metagenomes by means of the available sequence data alo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150712364
更新日期:2014-07-14 00:00:00
abstract::Rhizobia are Gram-negative bacteria that can exist either as free-living bacteria or as nitrogen-fixing symbionts inside root nodules of leguminous plants. The composition of the rhizobial outer surface, containing a variety of polysaccharides, plays a significant role in the adaptation of these bacteria in both habit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms12117898
更新日期:2011-01-01 00:00:00
abstract::Hyperhomocysteinemia (HHcy) exerts a wide range of biological effects and is associated with a number of diseases, including cardiovascular disease, dementia, neural tube defects, and cancer. Although mechanisms of HHcy toxicity are not fully uncovered, there has been a significant progress in their understanding. The...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20133140
更新日期:2019-06-27 00:00:00
abstract::Chemotherapy is one of the most common strategies for tumor treatment but often associated with post-therapy tumor recurrence. While chemotherapeutic drugs are known to induce tumor cell senescence, the roles and mechanisms of senescence in tumor recurrence remain unclear. In this study, we used doxorubicin to induce ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020849
更新日期:2021-01-15 00:00:00
abstract::Cell-to-cell communication mediates a plethora of cellular decisions and behaviors that are crucial for the correct and robust development of multicellular organisms. Many of these signals are encoded in secreted hormones or growth factors that bind to and activate cell surface receptors, to transmit the cue intracell...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21010188
更新日期:2019-12-26 00:00:00
abstract::Long-standing efforts to identify the multifaceted roles of histone deacetylase inhibitors (HDACis) have positioned these agents as promising drug candidates in combatting cancer, autoimmune, neurodegenerative, and infectious diseases. The same has also encouraged the evaluation of multiple HDACi candidates in preclin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20092241
更新日期:2019-05-07 00:00:00
abstract::Rheumatoid arthritis (RA) is a chronic, inflammatory, and systemic autoimmune disease that affects the connective tissue and primarily the joints. If not treated, RA ultimately leads to progressive cartilage and bone degeneration. The etiology of the pathogenesis of RA is unknown, demonstrating heterogeneity in its cl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21217916
更新日期:2020-10-25 00:00:00
abstract::Detergent-resistant membranes (DRMs) microdomains, or "raft lipids", are key components of the plasma membrane (PM), being involved in membrane trafficking, signal transduction, cell wall metabolism or endocytosis. Proteins imbibed in these domains play important roles in these cellular functions, but there are few st...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207694
更新日期:2020-10-17 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a leading cause of cancer-related morbidity and mortality worldwide. Most patients are diagnosed with advanced disease, limiting their options for treatment. While current treatments are adequate for lower staged disease, available systemic treatments are limited, with marginal benefi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21186757
更新日期:2020-09-15 00:00:00
abstract::Idiopathic pulmonary fibrosis (IPF) is age-related interstitial lung disease of unknown etiology. About 100,000 people in the U.S have IPF, with a 3-year median life expectancy post-diagnosis. The development of an effective treatment for pulmonary fibrosis will require an improved understanding of its molecular patho...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21020643
更新日期:2020-01-18 00:00:00
abstract::Protein self-association is a key feature that can modulate the physiological role of proteins or lead to deleterious effects when uncontrolled. Protein oligomerization is a simple way to modify the activity of a protein, as the modulation of binding interfaces allows for self-activation or inhibition, or variation in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17050655
更新日期:2016-04-30 00:00:00
abstract:BACKGROUND:Gadolinium chloride (GdCl3) has been reported to attenuate liver injury caused by a variety of toxicants. Gap junctional intercellular communication (GJIC) is thought to be essential in controlling liver homeostasis and pathology. Here we evaluate the effects of GdCl3 on functional GJIC and connexin expressi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20153748
更新日期:2019-07-31 00:00:00
abstract::A series of novel purpurinimides with long wavelength absorption were designed and synthesized to develop novel and potential photosensitizers. These compounds were investigated through reduction, oxidation, rearrangement reaction and amidation reactions of methyl pheophorbide a. They demonstrated a considerable batho...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15058091
更新日期:2014-05-08 00:00:00
abstract::The aim of this study was to investigate the chemical composition of acetone extracts of the lichens Toninia candida and Usnea barbata and in vitro antioxidant, antimicrobial, and anticancer activities of these extracts together with some of their major metabolites. The chemical composition of T. candida and U. barbat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131114707
更新日期:2012-11-12 00:00:00
abstract::There is evidence that spaceflight poses acute and late risks to the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched from the Kennedy Space Center (KSC) on a 13-day mission. Within 3⁻5 h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010007
更新日期:2018-12-20 00:00:00
abstract::Ultraviolet radiation (UV) is a major causative factor of DNA damage, inflammatory responses, reactive oxygen species (ROS) generation and a turnover of various cutaneous lesions resulting in skin photoaging. The purpose of this study is to investigate the protective effect of protocatechuic aldehyde (PA), which is a ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134619
更新日期:2020-06-29 00:00:00
abstract::Aging is associated with a dysregulation of the immune system, leading to a general pro-inflammatory state of the organism, a process that has been named inflamm-aging. Oxidative stress has an important role in aging and in the regulation of immune responses, probably playing a role in the development of age-related d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18122612
更新日期:2017-12-05 00:00:00
abstract::A bstract: Quorum sensing (QS) enables single-celled bacteria to communicate with chemical signals in order to synchronize group-level bacterial behavior. Pseudoalteromonas are marine bacteria found in versatile environments, of which QS regulation for their habitat adaptation is extremely fragmentary. To distinguish ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113636
更新日期:2018-11-18 00:00:00
abstract::Molecular defects in type 1 facioscapulohumeral muscular dystrophy (FSHD) are caused by a heterozygous contraction of the D4Z4 repeat array from 1 to 10 repeat units (RUs) on 4q35. This study compared (1) the phenotype and severity of FSHD1 between patients carrying 6-8 vs. 9-10 RUs, (2) the amount of methylation in d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062221
更新日期:2020-03-23 00:00:00