Abstract:
BACKGROUND:Engineered nanomaterials may release nanosized residues, by degradation, throughout their life cycle. These residues may be a threat for living organisms. They may be ingested by humans through food and water. Although the toxicity of pristine CeO2 nanoparticles (NPs) has been documented, there is a lack of studies on manufactured nanoparticles, which are often surface modified. Here, we investigated the potential adverse effects of CeO2 Nanobyk 3810™ NPs, used in wood care, and their residues, altered by light or acid. RESULTS:Human intestinal Caco-2 cells were exposed to residues degraded by daylight or in a medium simulating gastric acidity. Size and zeta potential were determined by dynamic light scattering. The surface structure and redox state of cerium were analyzed by transmission electronic microscopy (TEM) and X-ray absorption spectroscopy, respectively. Viability tests were performed in Caco-2 cells exposed to NPs. Cell morphology was imaged with scanning electronic microscopy. Gene expression profiles obtained from cells exposed to NPs before and after their alteration were compared, to highlight differences in cellular functions.No change in the cerium redox state was observed for altered NPs. All CeO2 NPs suspended in the culture medium became microsized. Cytotoxicity tests showed no toxicity after Caco-2 cell exposure to these various NPs up to 170 μg/mL (24 h and 72 h). Nevertheless, a more-sensitive whole-gene-expression study, based on a pathway-driven analysis, highlighted a modification of metabolic activity, especially mitochondrial function, by altered Nanobyk 3810™. The down-regulation of key genes of this pathway was validated by qRT-PCR. Conversely, Nanobyk 3810™ coated with ammonium citrate did not display any adverse effect at the same concentration. CONCLUSION:The degraded nanoparticles were more toxic than their coated counterparts. Desorption of the outside layer was the most likely cause of this discrepancy in toxicity. It can be assumed that the safe design of engineered nanoparticles could include robust protective layers conferring on them greater resistance to alteration during their life cycle.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Fisichella M,Berenguer F,Steinmetz G,Auffan M,Rose J,Prat Odoi
10.1186/1471-2164-15-700subject
Has Abstractpub_date
2014-08-21 00:00:00pages
700issn
1471-2164pii
1471-2164-15-700journal_volume
15pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:Pantoea ananatis is found in a wide range of natural environments, including water, soil, as part of the epi- and endophytic flora of various plant hosts, and in the insect gut. Some strains have proven effective as biological control agents and plant-growth promoters, while other strains have been implicate...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-404
更新日期:2014-05-27 00:00:00
abstract:BACKGROUND:Macrosatellites are some of the largest variable number tandem repeats in the human genome, but what role these unusual sequences perform is unknown. Their importance to human health is clearly demonstrated by the 4q35 macrosatellite D4Z4 that is associated with the onset of the muscle degenerative disease f...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-632
更新日期:2010-11-15 00:00:00
abstract:BACKGROUND:Siraitia grosvenorii (Luohanguo) is an herbaceous perennial plant native to southern China and most prevalent in Guilin city. Its fruit contains a sweet, fleshy, edible pulp that is widely used in traditional Chinese medicine. The major bioactive constituents in the fruit extract are the cucurbitane-type tri...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-343
更新日期:2011-07-05 00:00:00
abstract:BACKGROUND:Nuclear receptors are hormone-regulated transcription factors whose signaling controls numerous aspects of development and physiology. Many receptors recognize DNA hormone response elements formed by direct repeats of RGKTCA motifs separated by 1 to 5 bp (DR1-DR5). Although many known such response elements ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-8-23
更新日期:2007-01-19 00:00:00
abstract:BACKGROUND:Leishmania (L) are intracellular protozoan parasites that are able to survive and replicate within the harsh and potentially hostile phagolysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MPhi) striving to eliminate the pathogen and the pa...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-238
更新日期:2008-05-21 00:00:00
abstract:BACKGROUND:Cardiorespiratory fitness (VO2max) is an excellent predictor of chronic disease morbidity and mortality risk. Guidelines recommend individuals undertake exercise training to improve VO2max for chronic disease reduction. However, there are large inter-individual differences between exercise training responses...
journal_title:BMC genomics
pub_type: 杂志文章,评审
doi:10.1186/s12864-017-4192-6
更新日期:2017-11-14 00:00:00
abstract:BACKGROUND:Helitrons are class-II eukaryotic transposons that transpose via a rolling circle mechanism. Due to their ability to capture and mobilize gene fragments, they play an important role in the evolution of their host genomes. We have used a bioinformatics approach for the identification of helitrons in two Pleur...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-1071
更新日期:2014-12-05 00:00:00
abstract:BACKGROUND:The pond snail Lymnaea stagnalis (L. stagnalis) has been widely used as a model organism in neurobiology, ecotoxicology, and parasitology due to the relative simplicity of its central nervous system (CNS). However, its usefulness is restricted by a limited availability of transcriptome data. While sequence i...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-07287-2
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:Alternative splicing (AS) is a central mechanism in the generation of genomic complexity and is a major contributor to transcriptome and proteome diversity. Alterations of the splicing process can lead to deregulation of crucial cellular processes and have been associated with a large spectrum of human disea...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-S5-S4
更新日期:2010-12-22 00:00:00
abstract:BACKGROUND:Colon cancer occurrence is increasing worldwide, making it the third most frequent cancer. Although many therapeutic options are available and quite efficient at the early stages, survival is strongly decreased when the disease has spread to other organs. The identification of molecular markers of colon canc...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-4139-y
更新日期:2017-09-30 00:00:00
abstract:BACKGROUND:Lipopolysaccharide (LPS) from Gram-negative bacteria cause innate immune responses in animals and plants. The molecules involved in LPS signaling in animals are well studied, whereas those in plants are not yet as well documented. Recently, we identified Arabidopsis AtLBR-2, which binds to LPS from Pseudomon...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-4372-4
更新日期:2017-12-29 00:00:00
abstract:BACKGROUND:Palmitoylation is a reversible post-translational protein modification which involves the addition of palmitate to cysteine residues. Palmitoylation is catalysed by the DHHC family of palmitoyl-acyl transferases (PATs) and reversibility is conferred by palmitoyl-protein thioesterases (PPTs). Mutations in gen...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-841
更新日期:2014-10-02 00:00:00
abstract:BACKGROUND:The floral transition plays a vital role in the life of ornamental plants. Despite progress in model plants, the molecular mechanisms of flowering regulation remain unknown in perennial plants. Rosa chinensis 'Old Blush' is a unique plant that can flower continuously year-round. In this study, gene expressio...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3584-y
更新日期:2017-02-23 00:00:00
abstract:BACKGROUND:'Systems-wide' approaches such as microarray RNA-profiling are ideally suited to the study of the complex overlapping responses of plants to biotic and abiotic stresses. However, commercial microarrays are only available for a limited number of plant species and development costs are so substantial as to be ...
journal_title:BMC genomics
pub_type: 杂志文章,meta分析
doi:10.1186/1471-2164-10-436
更新日期:2009-09-16 00:00:00
abstract:BACKGROUND:Accurate HLA typing at amino acid level (four-digit resolution) is critical in hematopoietic and organ transplantations, pathogenesis studies of autoimmune and infectious diseases, as well as the development of immunoncology therapies. With the rapid adoption of genome-wide sequencing in biomedical research,...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-325
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:In recent years, the number of human infections caused by opportunistic pathogens has increased dramatically. Plant rhizospheres are one of the most typical natural reservoirs for these pathogens but they also represent a great source for beneficial microbes with potential for biotechnological applications. ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-482
更新日期:2014-06-18 00:00:00
abstract:BACKGROUND:Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a comple...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-952
更新日期:2014-11-03 00:00:00
abstract:BACKGROUND:Small insertions and deletions (InDels) constitute the second most abundant class of genetic variants and have been found to be associated with many traits and diseases. The present study reports on the detection and characterisation of about 883 K high quality InDels from the whole-genome analysis of severa...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1711-1
更新日期:2015-07-31 00:00:00
abstract:BACKGROUND:The only known albino gorilla, named Snowflake, was a male wild born individual from Equatorial Guinea who lived at the Barcelona Zoo for almost 40 years. He was diagnosed with non-syndromic oculocutaneous albinism, i.e. white hair, light eyes, pink skin, photophobia and reduced visual acuity. Despite previo...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-363
更新日期:2013-05-31 00:00:00
abstract:BACKGROUND:The Chinese Taihu is one of the most prolific pig breeds in the world, which farrows at least five more piglets per litter than Western pig breeds partly due to a greater ovulation rate. Variation of ovulation rate maybe associated with the differences in the transcriptome of Chinese Taihu and Large White ov...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-111
更新日期:2011-02-16 00:00:00
abstract:BACKGROUND:The MHC and KIR loci are clinically relevant regions of the genome. Typing the sequence of these loci has a wide range of applications including organ transplantation, drug discovery, pharmacogenomics and furthering fundamental research in immune genetics. Rapid advances in biochemical and next-generation se...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1949-7
更新日期:2015-11-05 00:00:00
abstract:BACKGROUND:Cichlid fishes have evolved remarkably diverse reproductive, social, and feeding behaviors. Cell-to-cell signaling molecules, notably neuropeptides and peptide hormones, are known to regulate these behaviors across vertebrates. This class of signaling molecules derives from prohormone genes that have undergo...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2914-9
更新日期:2016-08-19 00:00:00
abstract:BACKGROUND:ATP binding cassette (ABC) systems are responsible for the import and export of a wide variety of molecules across cell membranes and comprise one of largest protein superfamilies found in prokarya, eukarya and archea. ABC systems play important roles in bacterial lifestyle, virulence and survival. In this s...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-8-83
更新日期:2007-03-28 00:00:00
abstract:BACKGROUND:Chemosensory receptors including olfactory receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) play a central role in sensing chemical signals and guiding insect behaviours, and are potential target genes in insect pest control. The cotton bollworm Helicoverpa armigera is one of the mos...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-597
更新日期:2014-07-15 00:00:00
abstract:BACKGROUND:Grass carp (Ctenopharyngodon idella) belongs to the family Cyprinidae which includes more than 2000 fish species. It is one of the most important freshwater food fish species in world aquaculture. A linkage map is an essential framework for mapping traits of interest and is often the first step towards under...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-135
更新日期:2010-02-24 00:00:00
abstract:BACKGROUND:Meiotic recombination is a fundamental evolutionary process. It not only generates diversity, but influences the efficacy of natural selection and genome evolution. There can be significant heterogeneity in recombination rates within and between species, however this variation is not well understood outside ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2884-y
更新日期:2016-08-09 00:00:00
abstract:BACKGROUND:The human zinc finger protein 191 (ZNF191) is a member of the SCAN domain family of Krüppel-like zinc finger transcription factors. ZNF191 shows 94% identity to its mouse homologue zinc finger protein 191(Zfp191), which is the most highly conserved among the human-mouse SCAN family member orthologues pairs. ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-241
更新日期:2009-05-22 00:00:00
abstract:BACKGROUND:Cell lines are an indispensable tool in biomedical research and often used as surrogates for tissues. Although there are recognized important cellular and transcriptomic differences between cell lines and tissues, a systematic overview of the differences between the regulatory processes of a cell line and th...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-4111-x
更新日期:2017-09-12 00:00:00
abstract:BACKGROUND:Xanthomonas citri pv. citri (Xcc) is a citrus canker causing Gram-negative bacteria. Currently, little is known about the biological and molecular responses of Xcc to low temperatures. RESULTS:Results depicted that low temperature significantly reduced growth and increased biofilm formation and unsaturated ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6193-0
更新日期:2019-11-06 00:00:00
abstract:BACKGROUND:Molecular characterization is important for efficient utilization of germplasm and development of improved varieties. In the present study, we investigated the genetic purity, relatedness and population structure of 265 maize inbred lines from the Ethiopian Institute of Agricultural Research (EIAR), the Inte...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-4173-9
更新日期:2017-10-12 00:00:00