Abstract:
:Neurofibromatosis type 1 (NF1) caused by NF1 gene mutation is a commonly inherited autosomal dominant disorder. Malignant peripheral nerve sheath tumors (MPNSTs), a type of aggressive sarcoma, are a major cause of mortality in NF1 patients. The malignant transformation of benign plexiform neurofibromas (PNs) to MPNSTs is a marked peculiarity in NF1 patients, yet the pathogenesis remains poorly understood. We found that an actin-associated protein transgelin (SM22) was highly expressed in NF1-deficient MPNST tissues compared to NF1-deficient PN tissues using immunohistological staining and primary cultured MPNST cells in western blot analysis. We further found that this transgelin upregulation was caused by increased transcriptional expression of the TAGLN gene encoding transgelin. Comparison of DNA methylation values in the promoter and subpromoter regions of the TAGLN gene in three types of NF1-deficient primary-cultured cells, derived from an NF1 patient's normal phenotype, a benign PN and MPNST tissues, revealed that the TAGLN gene was hypomethylated in the MPNST cells. Next, to determine the functional role of transgelin in MPNST pathogenesis, we manipulated the TAGLN gene expression and investigated the alteration of the RAS-mitogen-activated protein kinase (MAPK) signaling pathway in the normal-phenotypic and malignant tumor cells. The downregulation of TAGLN expression in NF1-deficient MPNST tumor cells through the treatment of the small interfering RNA resulted in a decrease in the RAS activation (GTP-RAS) and the downstream ERK1/2 activation (phosphorylated ERK1/2), while the overexpression of TAGLN in normal-phenotypic NF1-deficient cells caused an increase in RAS and ERK1/2 activation. These results indicate that upregulation of transgelin caused by hypomethylation of the TAGLN gene is closely involved in tumor progression in NF1.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Park GH,Lee SJ,Yim H,Han JH,Kim HJ,Sohn YB,Ko JM,Jeong SYdoi
10.3892/or.2014.3379subject
Has Abstractpub_date
2014-10-01 00:00:00pages
1347-54issue
4eissn
1021-335Xissn
1791-2431journal_volume
32pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Mammary stem cells (MSCs) are the progenitor population for human breast epithelia. MSCs give rise during mammary gland development to estrogen receptor (ER)-negative basal cells and the ER- luminal progenitor (LP) population which maintains ER+ and ER- luminal cells. The MSC population is expanded and tumorigenic in ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4003
更新日期:2015-07-01 00:00:00
abstract::It is well known that crucifers have antitumor effects and 3,3'-diindolylmethane (DIM) is one of the major bioactive components, and the associated molecular mechanisms in a short-term high-dose manner are widely discussed. However, the antitumor effects of DIM in a long-term low-dose manner in nasopharyngeal carcinom...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4428
更新日期:2016-02-01 00:00:00
abstract::EVL belongs to the Ena/VASP (Enabled/vasodilator-stimulated phosphoprotein) family of proteins [Mena (mammalian Ena), VASP and EVL], which have a range of roles in regulating the actin cytoskeleton. Growing evidence suggests that Mena and VASP are involved in carcinogenesis, though little is known about the significan...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::The antitumor effect of the human umbilical vein endothelial cell (HUVEC) vaccine has been well documented; however, its anti‑angiogenic effects on human esophageal squamous cell carcinoma (ESCC) have yet to be studied. In the present study, a 'humanized' mouse model was established by transplanting NOD/SCID mice with...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6653
更新日期:2018-11-01 00:00:00
abstract::A novel core‑shell type thermo‑nanoparticle (CSTNP) co‑loaded with temozolomide (TMZ) and the fluorescein new indocyanine green dye IR820 (termed IT‑CSTNPs) was designed and combined with a near‑infrared (NIR) laser to realize its photothermal conversion. The IT‑CSTNPs were prepared using a two‑step synthesis method a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7329
更新日期:2019-12-01 00:00:00
abstract::Complement system activation contributes to various immune and inflammatory diseases, as well as cancers.However, the role of complement activation in the proliferation of cancer cells is not clear. In the present study, we investigated the consequences of complement activation on the proliferation of breast cancer ce...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3489
更新日期:2014-12-01 00:00:00
abstract::Apoptosis is a crucial event for anticancer drug efficacy. The signal pathways activated by anticancer drugs are classified as death receptor (DR) -dependent or -independent. The mechanisms by which anticancer drugs induce apoptosis via DR-dependent pathways are not fully understood. In the present study, we assessed ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Signaling pathways that activate mTOR (mammalian target of rapamycin) are altered in many human cancers and these alterations are associated with prognosis and treatment response. mTOR inhibition can restore sensitivity to DNA damaging agents such as cisplatin. The rapamycin derivat...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1666
更新日期:2012-05-01 00:00:00
abstract::The aim of this study was to investigate the status of the c‑Myc‑related molecule Mina53 and the clinical impact of Mina53 nuclear localization in patients with stage II and III colorectal cancer (CRC). Patients (n=250) who underwent complete resection of CRC at our department were enrolled in this study, and tissue m...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6407
更新日期:2018-07-01 00:00:00
abstract::The present study aimed to investigate the role of miR‑181a in multiple myeloma (MM) cell lines. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect the expression of microRNA (miR)‑181a. The MM cell line RPMI 8226 stably transduced with miR‑181a mimics or inhibitor was establishe...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7160
更新日期:2019-07-01 00:00:00
abstract::DNA intrastrand cross-linking agents such as oxaliplatin induce DNA double-strand breaks (DSBs) during DNA repair and replication. In the present study, we hypothesized that DNA intrastrand cross-linking agents may significantly benefit colorectal cancer patients with deficiencies in DSB repair. Seventy-eight patients...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4488
更新日期:2016-03-01 00:00:00
abstract::ZNF703, a member of the NET/Nlz family of zinc finger transcription factors, contributes to aspects of developmental growth and patterning across evolutionarily diverse species. ZNF703 has been identified as a novel oncogene in human breast cancer. In the present study, we investigated the expression of ZNF703 in gast...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.2997
更新日期:2014-04-01 00:00:00
abstract::Oxymatrine, the main alkaloid component in the traditional Chinese herbal medicine Sophora japonica (Sophora flavescens Ait), has been reported to have antitumor properties. However, the mechanisms of action in human pancreatic cancer are not well established to date. In the present study, we investigated the antiangi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2529
更新日期:2013-08-01 00:00:00
abstract::Ligularia fischeri (LF) has been used as an edible herb and traditional medicine for the treatment of inflammatory and infectious diseases. In the present study, we report the effects and molecular mechanism of the ethanolic extract of LF on cell proliferation, invasion and tube formation in human umbilical vein endot...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4000
更新日期:2015-07-01 00:00:00
abstract::The tumor necrosis factor receptor (TNFR)-associated factor 4 (TRAF4) is a member of TRAF family proteins that act as major signal transducers of the TNF receptor and the interleukin-1 receptor/Toll-like receptor (IL-1R/TLR) superfamily. TRAF4 has been reported to be overexpressed in various human cancers. However, th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2789
更新日期:2013-12-01 00:00:00
abstract::As a common malignancy of the endocrine system, papillary thyroid carcinoma (PTC) seriously affects the quality of life of patients. lncRNA PAX8‑AS1:28, or lnc‑PSD4‑1:14 has been reported to be abnormally expressed in PTC. However, the function of PAX8‑AS1:28 in PTC is still unknown. Therefore, the present study aimed...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.6996
更新日期:2019-04-01 00:00:00
abstract::Multicellular tumor spheroids have been used to examine aspects of combined modality treatment since they often recreate the in vivo tumor environment much more closely than other models. The radioenhancement by gemcitabine (dFdC) on human glioma spheroids derived from cell lines (CLS) and biopsy tissue, grown as orga...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The use of prognostic markers for breast cancer allows therapeutic strategies to be defined more efficiently. The expression of glutathione (GSH) and glutathione peroxidase (GPX) in tumor cells has been evaluated as a predictor of prognosis and response to cytotoxic treatments. Its immunoexpression was assessed in 63 ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2540
更新日期:2013-09-01 00:00:00
abstract::One of the most important clinical problems in the treatment of human solid carcinoma is the intrinsic/acquired resistance. Cisplatin is a platinum compound that is one of the most effective agents in clinic. Copper-transporting P-type adenosine triphosphate (ATP7B) has been reported to be associated with cisplatin re...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.6.1285
更新日期:2001-11-01 00:00:00
abstract::Tetraspanins are transmembrane adaptor proteins involved in the regulation of various fundamental cellular processes. For a number of malignant diseases, the level of expression of members of the tetraspanin family was found to correlate with tumor cell invasiveness, ability to form metastases, and poor clinical outco...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::Dendritic cells (DCs) as 'professional' antigen-presenting cells (APCs) initiate and regulate immune responses to various antigens. DC-based vaccines have become a promising modality in cancer immunotherapy. Cytokeratin 19 (CK19) protein is expressed at high levels in lung cancer and many other tumor cells, suggesting...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4231
更新日期:2015-11-01 00:00:00
abstract::Signal transducer and activator of transcription 3 (STAT3) is an oncogene that promotes cell survival, proliferation, and motility. In the present study, we explored the mechanism involved in the inhibition by epigallocatechin-3-gallate (EGCG) of STAT3 signaling as detected by surface plasmon resonance (SPR)-binding a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2743
更新日期:2013-12-01 00:00:00
abstract::Chemokines have been reported to play crucial roles in tumor progression. Eotaxin-1 (CCL11), a member of the CC chemokine family, is elevated in many types of human cancer. Here, to reveal the molecular mechanisms of eotaxin-1 in prostate cancer cell invasion, the expression of eotaxin-1 receptors [CC chemokine recept...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3060
更新日期:2014-05-01 00:00:00
abstract::Interleukin‑9 (IL‑9) can function as both a positive and negative regulator of immune response, however the role of IL‑9 in tumor immunity is poorly understood. Chronic lymphocytic leukemia (CLL) is the most common chronic lympho‑proliferative disorder. Twenty CLL patients from 2010 to 2011 were recruited in the study...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6367
更新日期:2018-06-01 00:00:00
abstract::The progress of the application of adenovirus in cancer gene therapy is hindered by the lack of expression of native adenovirus receptor on a variety of cancer types. Hence, strategies are needed to retarget the adenoviral vector to non-native cellular surface receptors. In the present study, a new peptide SWDIAWPPLKV...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3065
更新日期:2014-05-01 00:00:00
abstract::Improvements in detection, treatment and prognosis for patients with non-small cell lung cancer (NSCLC) depend on the molecular understanding of tumor development and progression. Using Affymetrix GeneChips comprising 59,620 elements, we determined the gene expression profiles of 89 NSCLC and 15 normal lung samples. W...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract::MicroRNA-17-5p has been proven upregulated in many human malignancies and correlated with tumor progression. However, its expression and clinical significance in osteosarcoma is still unclear. Thus, the aim of the present study was to explore the effects of miR-17-5p in osteosarcoma tumorigenesis and development. The ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4542
更新日期:2016-03-01 00:00:00
abstract::Daily vs. weekly dosing with EGFR inhibitors (gefitinib and lapatinib) and an AKT inhibitor (MK2206) were compared in two rodent breast cancer models. Female Sprague-Dawley rats were administered methylnitrosourea (MNU) at 50 days of age, and gefitinib (daily/weekly dosing at 10/70 mg/kg BW) or lapatinib (daily/weekly...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6313
更新日期:2018-09-01 00:00:00
abstract::Butyrate was shown to have a preventive effect on colon cancer in vivo. Germinated barley foodstuff (GBF) was in a prebiotic stage and had the potency to attenuate mucosal inflammation and to increase fecal butyrate production in colitis. This study aimed to determine whether the GBF treatment in a colon cancer model ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-10-01 00:00:00
abstract::Gastric cancer (GC) is a severe public health problem worldwide, particularly in China. Radiotherapy is the main locoregional treatment for various types of unresectable tumor, including GC. However, many patients fail to respond to radiotherapy due to the intrinsic radioresistance of cancer cells. This study was desi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7187
更新日期:2019-08-01 00:00:00