The protein transportation pathway from Golgi to vacuoles via endosomes plays a role in enhancement of methylmercury toxicity.

Abstract:

:Methylmercury causes serious damage to the central nervous system, but the molecular mechanisms of methylmercury toxicity are only marginally understood. In this study, we used a gene-deletion mutant library of budding yeast to conduct genome-wide screening for gene knockouts affecting the sensitivity of methylmercury toxicity. We successfully identified 31 genes whose deletions confer resistance to methylmercury in yeast, and 18 genes whose deletions confer hypersensitivity to methylmercury. Yeast genes whose deletions conferred resistance to methylmercury included many gene encoding factors involved in protein transport to vacuoles. Detailed examination of the relationship between the factors involved in this transport system and methylmercury toxicity revealed that mutants with loss of the factors involved in the transportation pathway from the trans-Golgi network (TGN) to the endosome, protein uptake into the endosome, and endosome-vacuole fusion showed higher methylmercury resistance than did wild-type yeast. The results of our genetic engineering study suggest that this vesicle transport system (proteins moving from the TGN to vacuole via endosome) is responsible for enhancing methylmercury toxicity due to the interrelationship between the pathways. There is a possibility that there may be proteins in the cell that enhance methylmercury toxicity through the protein transport system.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Hwang GW,Murai Y,Takahashi T,Naganuma A

doi

10.1038/srep05888

subject

Has Abstract

pub_date

2014-07-30 00:00:00

pages

5888

issn

2045-2322

pii

srep05888

journal_volume

4

pub_type

杂志文章
  • Design of a Broadband Solar Thermal Absorber Using a Deep Neural Network and Experimental Demonstration of Its Performance.

    abstract::In using nanostructures to design solar thermal absorbers, computational methods, such as rigorous coupled-wave analysis and the finite-difference time-domain method, are often employed to simulate light-structure interactions in the solar spectrum. However, those methods require heavy computational resources and CPU ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51407-2

    authors: Seo J,Jung PH,Kim M,Yang S,Lee I,Lee J,Lee H,Lee BJ

    更新日期:2019-10-21 00:00:00

  • Probing the dielectric, piezoelectric and magnetic behavior of CoFe2O4/BNT-BT0.08 composite thin film fabricated by sol-gel and spin-coating methods.

    abstract::We investigated in this paper a novel bilayer composite obtained by sol-gel and spin coating of the ferroelectric 0.92Na0.5Bi0.5TiO3-0.08BaTiO3 (abbreviated as BNT-BT0.08) and ferromagnetic CoFe2O4 phases, for miniature low-frequency magnetic sensors and piezoelectric sensors. This heterostructure, deposited on Si-Pt ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36232-3

    authors: Cernea M,Vasile BS,Surdu VA,Trusca R,Bartha C,Craciun F,Galassi C

    更新日期:2018-12-14 00:00:00

  • p53 functional states are associated with distinct aldehyde dehydrogenase transcriptomic signatures.

    abstract::p53 and aldehyde dehydrogenase (ALDH) have been implicated in key tumorigenesis processes including cancer initiating cell (CIC) maintenance; however, the relationship between these two mediators remains poorly defined. In this study, ALDH isoform expression diversity was revealed in CICs with disparate p53 functional...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-57758-5

    authors: Gui S,Xie X,O'Neill WQ,Chatfield-Reed K,Yu JG,Teknos TN,Pan Q

    更新日期:2020-01-23 00:00:00

  • YIP1 family member 4 (YIPF4) is a novel cellular binding partner of the papillomavirus E5 proteins.

    abstract::E5 proteins are amongst the least understood of the Human Papillomavirus (HPV) encoded gene products. They are small, membrane-integrated proteins known to modulate a number of critical host pathways associated with pathogenesis including growth factor receptor signaling and immune evasion. Their role in the virus lif...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12523

    authors: Müller M,Wasson CW,Bhatia R,Boxall S,Millan D,Goh GY,Haas J,Stonehouse NJ,Macdonald A

    更新日期:2015-08-03 00:00:00

  • Relationship Between Homodimeric Glucocorticoid Receptor and Transcriptional Regulation Assessed via an In Vitro Fluorescence Correlation Spectroscopy-Microwell System.

    abstract::Glucocorticoid receptor (GR) is a hormone-activated transcription regulatory protein involved in metabolism as well as adrenocortical responses to psychosocial stress. Ligand-activated GR localizes to the nucleus, where GR homodimers regulate gene transcription via direct binding to glucocorticoid response elements (G...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-25393-w

    authors: Oasa S,Mikuni S,Yamamoto J,Kurosaki T,Yamashita D,Kinjo M

    更新日期:2018-05-10 00:00:00

  • HilD induces expression of a novel Salmonella Typhimurium invasion factor, YobH, through a regulatory cascade involving SprB.

    abstract::HilD is an AraC-like transcriptional regulator encoded in the Salmonella pathogenicity island 1 (SPI-1), which actives transcription of many genes within and outside SPI-1 that are mainly required for invasion of Salmonella into host cells. HilD controls expression of target genes directly or by acting through distinc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49192-z

    authors: Banda MM,Manzo R,Bustamante VH

    更新日期:2019-09-04 00:00:00

  • Cell sorting in a Petri dish controlled by computer vision.

    abstract::Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-contr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01088

    authors: Környei Z,Beke S,Mihálffy T,Jelitai M,Kovács KJ,Szabó Z,Szabó B

    更新日期:2013-01-01 00:00:00

  • Investigating Cortical Inhibition in First-Degree Relatives and Probands in Schizophrenia.

    abstract::Deficits in GABAergic inhibitory neurotransmission are a reliable finding in schizophrenia (SCZ) patients. Previous studies have reported that unaffected first-degree relatives of patients with SCZ demonstrate neurophysiological abnormalities that are intermediate between probands and healthy controls. In this study, ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43629

    authors: Radhu N,Dominguez LG,Greenwood TA,Farzan F,Semeralul MO,Richter MA,Kennedy JL,Blumberger DM,Chen R,Fitzgerald PB,Daskalakis ZJ

    更新日期:2017-02-27 00:00:00

  • AutoImpute: Autoencoder based imputation of single-cell RNA-seq data.

    abstract::The emergence of single-cell RNA sequencing (scRNA-seq) technologies has enabled us to measure the expression levels of thousands of genes at single-cell resolution. However, insufficient quantities of starting RNA in the individual cells cause significant dropout events, introducing a large number of zero counts in t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-34688-x

    authors: Talwar D,Mongia A,Sengupta D,Majumdar A

    更新日期:2018-11-05 00:00:00

  • Microstructure and chemical composition of Roman orichalcum coins emitted after the monetary reform of Augustus (23 B.C.).

    abstract::A collection of ancient Roman orichalcum coins, i.e., a copper-zinc alloy, minted under the reigns from Caesar to Domitianus, have been characterised using scanning electron microscopy (SEM-EDS) and electron microprobe analysis (EMPA). We studied, for the first time, coins emitted by Romans after the reforms of August...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-48941-4

    authors: Di Fazio M,Felici AC,Catalli F,De Vito C

    更新日期:2019-09-03 00:00:00

  • Alleviation of depression-like behavior in a cystic fibrosis mouse model by Hdac6 depletion.

    abstract::Cystic fibrosis (CF) patients experience heightened levels of anxiety and depression. Stress from dealing with chronic disease and rigorous treatment regimens certainly are primary contributors to these outcomes. We previously have demonstrated that microtubule alterations in CF are linked to a number of CF phenotypes...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73298-4

    authors: Corey DA,Rymut SM,Kelley TJ

    更新日期:2020-10-01 00:00:00

  • Author Correction: Supersonic turbulent flow simulation using a scalable parallel modal discontinuous Galerkin numerical method.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-58144-x

    authors: Houba T,Dasgupta A,Gopalakrishnan S,Gosse R,Roy S

    更新日期:2020-01-21 00:00:00

  • Knockdown Indian Hedgehog (Ihh) does not delay Fibular Fracture Healing in genetic deleted Ihh mice and pharmaceutical inhibited Ihh Mice.

    abstract::The objective of this study was to determine if Ihh is required for fracture healing. Fibular fracture was created in adult Col2a1-CreERT2; Ihhfl/fl mice. Ihhfl/fl mice received Tamoxifen (TM) to delete Ihh. WT mice received Cyclopamine to inhibit Hh pathway. Callus tissue properties and Ihh pathway were analyzed at 1...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-28657-7

    authors: Li S,Xiang C,Wei X,Li H,Li K,Sun X,Wang S,Zhang M,Deng J,Wang X,Li P,Li R,Zhang Y,Wei L

    更新日期:2018-07-09 00:00:00

  • Comparative genomics provides insights into the lifestyle and reveals functional heterogeneity of dark septate endophytic fungi.

    abstract::Dark septate endophytes (DSE) are a form-group of root endophytic fungi with elusive functions. Here, the genomes of two common DSE of semiarid areas, Cadophora sp. and Periconia macrospinosa were sequenced and analyzed with another 32 ascomycetes of different lifestyles. Cadophora sp. (Helotiales) and P. macrospinosa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24686-4

    authors: Knapp DG,Németh JB,Barry K,Hainaut M,Henrissat B,Johnson J,Kuo A,Lim JHP,Lipzen A,Nolan M,Ohm RA,Tamás L,Grigoriev IV,Spatafora JW,Nagy LG,Kovács GM

    更新日期:2018-04-20 00:00:00

  • Author Correction: Suppression of presbyopia progression with pirenoxine eye drops: experiments on rats and non-blinded, randomized clinical trial of efficacy.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-62436-7

    authors: Tsuneyoshi Y,Higuchi A,Negishi K,Tsubota K

    更新日期:2020-04-16 00:00:00

  • Author Correction: Correction-free force calibration for magnetic tweezers experiments.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-36219-0

    authors: Ostrofet E,Papini FS,Dulin D

    更新日期:2018-12-10 00:00:00

  • Coherently Tunable Triangular Trefoil Phaseonium Metamaterial.

    abstract::Phaseonium is a three-level Λ quantum system, in which a coherent microwave and an optical control (pump) beams can be used to actively modulate the dielectric response. Here we propose a new metamaterial structure comprising of a periodic array of triangular phaseonium metamolecules arranged as a trefoil. We present ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21083

    authors: Nguyen DM,Soci C,Ooi CH

    更新日期:2016-02-16 00:00:00

  • LFP beta amplitude is linked to mesoscopic spatio-temporal phase patterns.

    abstract::Beta oscillations observed in motor cortical local field potentials (LFPs) recorded on separate electrodes of a multi-electrode array have been shown to exhibit non-zero phase shifts that organize into planar waves. Here, we generalize this concept to additional classes of salient patterns that fully describe the spat...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22990-7

    authors: Denker M,Zehl L,Kilavik BE,Diesmann M,Brochier T,Riehle A,Grün S

    更新日期:2018-03-26 00:00:00

  • Interleukin-22 level is negatively correlated with neutrophil recruitment in the lungs in a Pseudomonas aeruginosa pneumonia model.

    abstract::Pseudomonas aeruginosa is a major threat for immune-compromised patients. Bacterial pneumonia can induce uncontrolled and massive neutrophil recruitment ultimately leading to acute respiratory distress syndrome and epithelium damage. Interleukin-22 plays a central role in the protection of the epithelium. In this stud...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11518-0

    authors: Broquet A,Jacqueline C,Davieau M,Besbes A,Roquilly A,Martin J,Caillon J,Dumoutier L,Renauld JC,Heslan M,Josien R,Asehnoune K

    更新日期:2017-09-08 00:00:00

  • Detection and measure of genuine tripartite entanglement with partial transposition and realignment of density matrices.

    abstract::It is challenging task to detect and measure genuine multipartite entanglement. We investigate the problem by considering the average based positive partial transposition(PPT) criterion and the realignment criterion. Sufficient conditions for detecting genuine tripartite entanglement are presented. We also derive lowe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-17585-7

    authors: Li M,Wang J,Shen S,Chen Z,Fei SM

    更新日期:2017-12-08 00:00:00

  • Clinical features of irreversible rejection after allogeneic uterus transplantation in cynomolgus macaques.

    abstract::Uterus transplantation (UTx) is a potential option for women with uterine factor infertility to have a child. The clinical features indicating irreversible rejection of the uterus are unknown. In our experimental series of allogeneic UTx in cynomolgus macaques, six female macaques were retrospectively examined, which ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70914-1

    authors: Kisu I,Emoto K,Masugi Y,Yamada Y,Matsubara K,Obara H,Matoba Y,Banno K,Kato Y,Saiki Y,Itagaki I,Kawamoto I,Iwatani C,Murase M,Nakagawa T,Tsuchiya H,Ishigaki H,Urano H,Ema M,Ogasawara K,Aoki D,Nakagawa K,Shiina

    更新日期:2020-08-17 00:00:00

  • Ecosystem functions including soil organic carbon, total nitrogen and available potassium are crucial for vegetation recovery.

    abstract::The effects of biodiversity on ecosystem functions have been extensively studied, but little is known about the effects of ecosystem functions on biodiversity. This knowledge is important for understanding biodiversity-ecosystem functioning relationships. Desertification reversal is a significant global challenge, but...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-25875-x

    authors: Qiu K,Xie Y,Xu D,Pott R

    更新日期:2018-05-15 00:00:00

  • Difficidin and bacilysin from Bacillus amyloliquefaciens FZB42 have antibacterial activity against Xanthomonas oryzae rice pathogens.

    abstract::Bacterial blight and bacterial leaf streak are serious, economically damaging, diseases of rice caused by the bacteria Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola. Bacillus amyloliquefaciens FZB42 was shown to possess biocontrol activity against these Xanthomonas strains by producing the antibiotic compo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12975

    authors: Wu L,Wu H,Chen L,Yu X,Borriss R,Gao X

    更新日期:2015-08-13 00:00:00

  • Seabird distribution patterns observed with fishing vessel's radar reveal previously undescribed sub-meso-scale clusters.

    abstract::Seabirds are known to concentrate on prey patches or at predators aggregations standing for potential feeding opportunities. They may search for prey using olfaction or by detecting visually feeding con-specifics and sub-surface predators, or even boats. Thus, they might form a foraging network. We hypothesized that c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07480-6

    authors: Assali C,Bez N,Tremblay Y

    更新日期:2017-08-04 00:00:00

  • Axin2-expressing cells differentiate into reparative odontoblasts via autocrine Wnt/β-catenin signaling in response to tooth damage.

    abstract::In non-growing teeth, such as mouse and human molars, primary odontoblasts are long-lived post-mitotic cells that secrete dentine throughout the life of the tooth. New odontoblast-like cells are only produced in response to a damage or trauma. Little is known about the molecular events that initiate mesenchymal stem c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03145-6

    authors: Babb R,Chandrasekaran D,Carvalho Moreno Neves V,Sharpe PT

    更新日期:2017-06-08 00:00:00

  • STAT3 signaling stimulates miR-21 expression in bovine cumulus cells during in vitro oocyte maturation.

    abstract::MicroRNAs are potent regulators of gene expression that have been widely implicated in reproduction and embryo development. Recent studies have demonstrated that miR-21, a microRNA extensively studied in the context of disease, is important in multiple facets of reproductive biology including folliculogenesis, ovulati...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29874-w

    authors: Tscherner A,Brown AC,Stalker L,Kao J,Dufort I,Sirard MA,LaMarre J

    更新日期:2018-08-01 00:00:00

  • Absorption of α-tocopheryl acetate is limited in mink kits (Mustela vison) during weaning.

    abstract::Bioavailability of α-tocopherol varies with source, dose and duration of supplementation. The effect of source and dose of α-tocopherol on response of α-tocopherol stereoisomers in plasma and tissues of mink kits during the weaning period was studied. Twelve mink kits were euthanised in CO2 at the beginning of the exp...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80902-0

    authors: Lashkari S,Clausen TN,Foldager L,Jensen SK

    更新日期:2021-01-29 00:00:00

  • 50 Years of Cetacean Strandings Reveal a Concerning Rise in Chilean Patagonia.

    abstract::Cetacean strandings (CS) have been reported in increasing numbers in coastal areas worldwide. Although the causes of these strandings are unknown, a number of anthropogenic and environmental factors have been suggested. This paper aims to characterize CS patterns and describe their fine-scale spatiotemporal dynamics. ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-66484-x

    authors: Alvarado-Rybak M,Toro F,Escobar-Dodero J,Kinsley AC,Sepúlveda MA,Capella J,Azat C,Cortés-Hinojosa G,Zimin-Veselkoff N,Mardones FO

    更新日期:2020-06-11 00:00:00

  • Using stable isotopes to analyse extinction risks and reintroduction opportunities of native species in invaded ecosystems.

    abstract::Invasive non-native species have pervasive impacts on native biodiversity, including population extirpations and species extinctions. Identifying reasons why a population of a native species is extirpated following an invasion often relies on literature-based results of anecdotal observations. The well-established sch...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78328-9

    authors: Haubrock PJ,Balzani P,Britton JR,Haase P

    更新日期:2020-12-10 00:00:00

  • Topological constraints of structural elements in regulation of catalytic activity in HDV-like self-cleaving ribozymes.

    abstract::Self-cleaving ribozymes fold into intricate structures, which orient active site groups into catalytically competent conformations. Most ribozyme families have distinct catalytic cores stabilized by tertiary interactions between domains peripheral to those cores. We show that large hepatitis delta virus (HDV)-like rib...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28179

    authors: Webb CH,Nguyen D,Myszka M,Lupták A

    更新日期:2016-06-15 00:00:00