Glycosaminoglycan composition of PC12 pheochromocytoma cells: a comparison with PC12D cells, a new subline of PC12 cells.

abstract：:Catechol-O-methyltransferase (COMT) has both soluble (S-COMT) and membrane-bound (MB-COMT) isoforms. A specific COMT antibody was used in immunohistochemical and confocal co-localization studies to explore the distribution of COMT in general in normal mice and MB-COMT in particular, in an S-COMT deficient mouse line. ...

journal_title：Journal of neurochemistry

authors： Myöhänen TT,Schendzielorz N,Männistö PT

更新日期：2010-06-01 00:00:00

abstract：:Neuromodulation is a fundamental process in the brain that regulates synaptic transmission, neuronal network activity and behavior. Emerging evidence demonstrates that astrocytes, a major population of glial cells in the brain, play previously unrecognized functions in neuronal modulation. Astrocytes can detect the le...

journal_title：Journal of neurochemistry

authors： Fellin T

更新日期：2009-02-01 00:00:00

abstract：:Adrenal chromaffin cells release neurotransmitters in response to stress and may be involved in conditions such as post-traumatic stress and anxiety disorders. Neurotransmitter release is triggered, in part, by activation of nicotinic acetylcholine receptors (nAChRs). However, despite decades of use as a model system ...

journal_title：Journal of neurochemistry

authors： Hone AJ,Rueda-Ruzafa L,Gordon TJ,Gajewiak J,Christensen S,Dyhring T,Albillos A,McIntosh JM

更新日期：2020-07-01 00:00:00

abstract：:Heterozygous CB1 receptor knockout mice were used to examine the effect of reduced CB1 receptor density on G-protein activation in membranes prepared from four brain regions: cerebellum, hippocampus, striatum/globus pallidus (striatum/GP) and cingulate cortex. Results showed that CB1 receptor levels were approximately...

journal_title：Journal of neurochemistry

authors： Selley DE,Rorrer WK,Breivogel CS,Zimmer AM,Zimmer A,Martin BR,Sim-Selley LJ

更新日期：2001-05-01 00:00:00

abstract：:Dendritic trafficking and translation of brain-derived neurotrophic factor (BDNF) transcripts play a key role in mediating synaptic plasticity. Recently, we demonstrated that siRNA-mediated knockdown of translin, an RNA-binding protein, impairs KCl-induced dendritic trafficking of BDNF mRNA in cultured hippocampal neu...

journal_title：Journal of neurochemistry

authors： Wu YC,Williamson R,Li Z,Vicario A,Xu J,Kasai M,Chern Y,Tongiorgi E,Baraban JM

更新日期：2011-03-01 00:00:00

abstract：:We have investigated the transport characteristics of dehydroepiandrosterone sulfate (DHEAS), a neuroactive steroid, at the blood-brain barrier (BBB) in a series of functional in vivo and in vitro studies. The apparent BBB efflux rate constant of [(3)H]DHEAS evaluated by the brain efflux index method was 2.68 x 10(-2)...

journal_title：Journal of neurochemistry

authors： Asaba H,Hosoya K,Takanaga H,Ohtsuki S,Tamura E,Takizawa T,Terasaki T

更新日期：2000-11-01 00:00:00

abstract：:The contribution of neuromelanin (NM) to the pathogenesis of Parkinson's disease (PD) has long been suspected. In particular, a correlation has been reported between the estimated cell loss in the mesencephalic dopaminergic cell groups and the percentage of NM-pigmented neurons in these cell groups. To test whether th...

journal_title：Journal of neurochemistry

authors： Kastner A,Hirsch EC,Lejeune O,Javoy-Agid F,Rascol O,Agid Y

更新日期：1992-09-01 00:00:00

abstract：:The neurochemical profile of a new compound, Lu 19-005 [(+/-)trans-3-(3,4-dichlorophenyl)-N-methyl-1-indanamine hydrochloride], has been investigated. Lu 19-005 is a potent inhibitor of the synaptosomal uptake of 3,4-dihydroxyphenylethylamine (dopamine, DA), noradrenaline (NA), and 5-hydroxytryptamine (5-HT, serotonin...

journal_title：Journal of neurochemistry

authors： Hyttel J,Larsen JJ

更新日期：1985-05-01 00:00:00

abstract：:Myelin P2 protein is a small (14,800 Da) protein found in peripheral and central nervous system myelin. To investigate the regulation of expression of this myelin protein, a mouse genomic library was screened with a rabbit P2 cDNA (pSN2.2-2), and a single positive phage clone containing a 20-kb insert was obtained. Th...

journal_title：Journal of neurochemistry

authors： Narayanan V,Kaestner KH,Tennekoon GI

更新日期：1991-07-01 00:00:00

abstract：:Major depressive disorder is a common form of mental illness. Many brain regions are implicated in the pathophysiology and symptomatology of depression. Among key brain areas is the striatum that controls reward and mood and is involved in the development of core depression-like behavior in animal models of depression...

journal_title：Journal of neurochemistry

authors： Mao LM,Wang JQ

更新日期：2018-05-01 00:00:00

abstract：:This study demonstrates that in mammalian and nonmammalian species it is possible to deplete selectively and reversibly retinal glycinergic neurons of their content of glycine by exposure to sarcosine, a competitive inhibitor of glycine transporter 1 (glyt-1). This observation was used as a tool to test the hypothesis...

journal_title：Journal of neurochemistry

authors： Pow DV

更新日期：1998-06-01 00:00:00

abstract：:Microglial activation by blood-borne factors following blood-brain barrier damage may play a significant role in subsequent neuropathogenesis of several neurodegenerative diseases. Exposure of primary cultured rat brain microglia to pure, fatty acid- and lipid-deficient rat serum albumin or fraction V, (fatty acid and...

journal_title：Journal of neurochemistry

authors： Hooper C,Pinteaux-Jones F,Fry VA,Sevastou IG,Baker D,Heales SJ,Pocock JM

更新日期：2009-05-01 00:00:00

abstract：:Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate-saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent Km of approximately 1 mM. Maintenance of the cells in medium containing thyroid-hormone-free serum for 3, 6, or 9...

journal_title：Journal of neurochemistry

authors： Roeder LM,Williams IB,Tildon JT

更新日期：1985-11-01 00:00:00

abstract：:Considerable evidence indicates that the amyloid-beta (Abeta) peptide, a proteolytic fragment of the amyloid precursor protein, is the pathogenic agent in Alzheimer's disease (AD). A number of proteases have been reported as capable of degrading Abeta, among them: neprilysin, insulin-degrading enzyme, endothelin-conve...

journal_title：Journal of neurochemistry

authors： Yamin R,Bagchi S,Hildebrant R,Scaloni A,Widom RL,Abraham CR

更新日期：2007-01-01 00:00:00

abstract：:Both adenosine and interleukin-6 (IL-6) have been implicated in the pathophysiology of, e.g., epileptic seizures, traumatic brain injury, and affective disorders. Stimulation of adenosine A2B receptors on astrocytes in vitro leads to the increased synthesis and secretion of IL-6. We investigated whether or not activat...

journal_title：Journal of neurochemistry

authors： Vazquez JF,Clement HW,Sommer O,Schulz E,van Calker D

更新日期：2008-05-01 00:00:00

abstract：:Cyclic AMP phosphodiesterase activity in bovine brain coated vesicles displayed a Km of approximately 22 microM for cyclic AMP, a Vmax of 3.2 nmol/min/mg protein, and a Hill coefficient of 1.5, suggesting positive cooperativity. The enzyme activity was stimulated by cyclic GMP with maximal indexes of stimulation rangi...

journal_title：Journal of neurochemistry

authors： Silva WI,Schook W,Mittag TW,Puszkin S

更新日期：1986-04-01 00:00:00

abstract：:Spatial solute concentration profiles resulting from in vivo microdialysis were measured in rat caudate-putamen by quantitative autoradiography. Radiolabeled sucrose was included in the dialysate, and the tissue concentration profile measured after infusions of 14 min and 61.5 min in an acute preparation. In addition,...

journal_title：Journal of neurochemistry

authors： Dykstra KH,Hsiao JK,Morrison PF,Bungay PM,Mefford IN,Scully MM,Dedrick RL

更新日期：1992-03-01 00:00:00

abstract：:A dramatic loss of glutamate transport has been observed in sporadic amyotrophic lateral sclerosis and has been postulated to contribute to the disease. Experimentally, this hypothesis was corroborated by mimicking the chronic loss of glutamate transport in postnatal rat spinal cord organotypic cultures through the us...

journal_title：Journal of neurochemistry

authors： Rothstein JD,Kuncl RW

更新日期：1995-08-01 00:00:00

abstract：:Focal brain ischemia leads to a slow type of neuronal death in the penumbra that starts several hours after ischemia and continues to mature for days. During this maturation period, blood flow, cellular ATP and ionic homeostasis are gradually recovered in the penumbral region. In striking contrast, protein synthesis i...

journal_title：Journal of neurochemistry

authors： Zhang F,Liu CL,Hu BR

更新日期：2006-07-01 00:00:00

abstract：:Excitotoxicity mediated via the (S)-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) subtype of receptor for l-glutamate contributes to various neuropathologies involving acute brain injury and chronic degenerative disorders. In this study, AMPA-induced neuronal injury and staurosporine (STS)-mediated apopt...

journal_title：Journal of neurochemistry

authors： Beart PM,Lim ML,Chen B,Diwakarla S,Mercer LD,Cheung NS,Nagley P

更新日期：2007-12-01 00:00:00

abstract：:This review examines the involvement of nitrotyrosine as a marker for peroxynitrite-mediated damage in the dopamine neuronal system. We propose that the dopamine neuronal phenotype can influence the cytotoxic signature of peroxynitrite. Dopamine and tetrahydrobiopterin are concentrated in dopamine neurons, and both ar...

journal_title：Journal of neurochemistry

authors： Kuhn DM,Sakowski SA,Sadidi M,Geddes TJ

更新日期：2004-05-01 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative disorder characterized pathologically by motor neuron degeneration and associated with aggregation of RNA-binding proteins. TATA-binding protein-associated factor 15 (TAF15) accumulates as cytoplasmic aggregates in neuronal cells, and clearance of these...

journal_title：Journal of neurochemistry

authors： Choi HJ,Joo Cha S,Do HA,Kim HJ,Lee JW,Kim K

更新日期：2020-09-11 00:00:00

abstract：:We assessed the functional expression of the norepinephrine (NE) transporter (NET) in cultured rat cortical astrocytes. Specific [3H]NE uptake increased in a time-dependent manner, and this uptake involves temperature- and Na+-sensitive mechanisms. The Na+-dependent [3H]NE uptake was saturable, and the Km for the proc...

journal_title：Journal of neurochemistry

authors： Inazu M,Takeda H,Matsumiya T

更新日期：2003-01-01 00:00:00

abstract：:Ligand binding to the cannabinoid receptor of brain membranes has been characterized using [3H]CP 55,940 and the Multiscreen Filtration System. Binding of [3H]CP 55,940 is saturable and reaches equilibrium by 45 min at room temperature. At a concentration of 10 micrograms of membrane protein/well, the KD for [3H]CP 55...

journal_title：Journal of neurochemistry

authors： Hillard CJ,Edgemond WS,Campbell WB

更新日期：1995-02-01 00:00:00

abstract：:To examine whether the concentration gradient of glutamine (Gln) drives concentrative Na(+)-independent uptake of neutral amino acids (NAA) in mouse cerebral astrocytes, uptake was compared in "Gln-depleted" and "Gln-replete" cultures. Uptake (30 min in Na(+)-free buffer) of histidine, kynurenine, leucine, tyrosine, a...

journal_title：Journal of neurochemistry

authors： Brookes N

更新日期：1992-07-01 00:00:00

abstract：:The specific binding of [N-methyl-3H]flunitrazepam ([3H]FNZP) to a membrane fraction from the supraoesophageal ganglion of the locust (Schistocerca gregaria) has been measured. The ligand binds reversibly with a KD of 47 nM. The binding is Ca2+-dependent, a property not found for the equivalent binding site in vertebr...

journal_title：Journal of neurochemistry

authors： Robinson T,MacAllan D,Lunt G,Battersby M

更新日期：1986-12-01 00:00:00

abstract：:Abnormalities in the kynurenine pathway may play a role in Huntington's disease (HD). In this study, tryptophan depletion and loading were used to investigate changes in blood kynurenine pathway metabolites, as well as markers of inflammation and oxidative stress in HD patients and healthy controls. Results showed tha...

journal_title：Journal of neurochemistry

authors： Stoy N,Mackay GM,Forrest CM,Christofides J,Egerton M,Stone TW,Darlington LG

更新日期：2005-05-01 00:00:00

abstract：:Somatodendritic voltage-dependent K(+) currents (Kv4.2) channels mediate transient A-type K(+) currents and play critical roles in controlling neuronal excitability. Accumulating evidence has indicated that Kv4.2 channels are key regulatory components of the signaling pathways that lead to synaptic plasticity. In cont...

journal_title：Journal of neurochemistry

authors： Lei Z,Deng P,Xu ZC

更新日期：2008-07-01 00:00:00

abstract：:Antisera were raised in rabbits against the alpha subunit of a GTP-binding protein, Go. Because the antisera cross-reacted weakly with the alpha subunit of inhibitory GTP-binding protein of adenylate cyclase (Gi), they were purified with a Go alpha-coupled Sepharose column. Purified antibodies reacted only with Go alp...

journal_title：Journal of neurochemistry

authors： Asano T,Semba R,Ogasawara N,Kato K

更新日期：1987-05-01 00:00:00

abstract：:The turnover of acetylcholine (ACh) in rat brain synaptosomes and its compartmentation in the labile bound and stable bound pools were investigated. The P(2) fraction from rat brain was subjected to three sequential incubations, each terminated by centrifugation followed by determination of ACh concentrations by gas c...

journal_title：Journal of neurochemistry

authors： Jope RS

更新日期：1981-05-01 00:00:00