Farnesol induces apoptosis-like cell death in the pathogenic fungus Aspergillus flavus.

Abstract:

:Farnesol (FOH) is known to induce apoptosis in some fungi and mammalian cells. We treated Aspergillus flavus, one of the leading causes of human invasive aspergillosis and a key producer of the most potent naturally occurring hepatocarcinogenic compounds, with FOH to assess its effect on the viability of the fungus. FOH strongly inhibited germination and growth of A. flavus and induced markers for apoptosis including nuclear condensation, phosphatidylserine (PS) externalization, DNA fragmentation and intracellular reactive oxygen species (ROS) generation, metacaspase activation and abnormal cellular ultrastructure. Moreover, FOH-induced apoptosis in A. flavus was inhibited by the broad-spectrum caspase inhibitor Z-VAD-fmk and partially inhibited by the ROS scavenger l-proline, which suggests that FOH induces apoptosis in A. flavus via a mechanism involving metacaspase activation and ROS production.

journal_name

Mycologia

journal_title

Mycologia

authors

Wang X,Wang Y,Zhou Y,Wei X

doi

10.3852/13-292

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

881-8

issue

5

eissn

0027-5514

issn

1557-2536

pii

13-292

journal_volume

106

pub_type

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