Abstract：
:Penicillium janczewskii, isolated from the rhizosphere of Vernonia herbacea, grows rapidly on media containing either sucrose or inulin, although inulin more than sucrose induced the production of inulinases. Three different extracellular beta-fructofuranosidases (two inulinases and one invertase) were purified from fungal cultures grown on sucrose or inulin, through precipitation with ammonium sulfate, and anion-exchange, hydrophobic interaction and gel filtration chromatographies. The optimum temperature of the three enzymes was approximately 60 C, optimum pH 4-5.5 and apparent molecular mass of 80 kDa. K(m) and V(max) values determined for invertase on sucrose were respectively 3.7 10(-4) M and 7.9 10(-2) micromol/min/mL, and on inulin 6.3 10(-2) M and 2.09 10(-2) micromol/min/mL. The values of k(m) for the two inulinases were 8.11 10(-4) and 2.62 10(-3) M, being lower for inulin when compared to those obtained for sucrose. The inulinases did not produce oligofructans from inulin, indicating they are primarily exoinulinases. The differences found in inulinase induction patterns when inulin or sucrose was used seem to be related to modifications on the enzyme properties, mainly concerning substrate affinity.
journal_name
Mycologiajournal_title
Mycologiaauthors
Pessoni RA,Braga MR,Figueiredo-Ribeiro Rde Cdoi
10.3852/mycologia.99.4.493subject
Has Abstractpub_date
2007-07-01 00:00:00pages
493-503issue
4eissn
0027-5514issn
1557-2536journal_volume
99pub_type
杂志文章相关文献
MYCOLOGIA文献大全abstract：:Attachment of conidia on leaves is a critical first step in the life cycle of aquatic hyphomycetes in streams. In a first series of microcosm experiments, attachment success of three common aquatic hyphomycete species differing in conidial shape (compact, filiform and tetraradiate) was determined on two leaf species, ...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/mycologia.99.1.24
更新日期：2007-01-01 00:00:00
abstract：:We discovered that published polymerase chain reaction (PCR) assays for determining mating type (MAT) idiomorph failed to genotype some of the Fusarium fujikuroi species complex (FFSC) isolates recovered from Mangifera indica (mango), Swietenia macrophylla (big-leaf mahogany), Annona muricata (soursop), Bursera sp., a...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.1080/00275514.2019.1649956
更新日期：2019-09-01 00:00:00
abstract：:Russian knapweed (Rhaponticum repens) is a rangeland weed pest in the western United States. One candidate fungus for biological control of R. repens is Puccinia acroptili, which causes a rust disease. Understanding the life cycle of candidate rust fungi for weed biological control is an essential component in risk as...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/08-215
更新日期：2010-01-01 00:00:00
abstract：:During a survey of fungi associated with arthropods collected in a southern boreal mixed-wood forest in Alberta we obtained 29 isolates of a unique species of Leptographium. This species displayed a distinct combination of characteristics, including curved conidia on short-stipitate conidiophores, a secondary micronem...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/mycologia.98.5.771
更新日期：2006-09-01 00:00:00
abstract：:A new milkcap species, Lactarius fuscomarginatus, was found in the subtropical region of central Veracruz (eastern Mexico) associated with two relict populations of Fagus grandifolia var. mexicana. The species is characterized macroscopically by its dark pileus and stipe and by its distant and whitish lamellae with bl...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/11-051
更新日期：2012-01-01 00:00:00
abstract：:Protein fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI--TOF MS) is a rapid, reliable, and economical method to characterize isolates of terrestrial fungi and other microorganisms. The objective of our study was to evaluate the suitability of MALDI-TOF MS for th...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.1080/00275514.2018.1528129
更新日期：2019-01-01 00:00:00
abstract：:Germinating ascospores of Phaeocryptopus gaeumannii produce suprastomatal appressoria from which penetration pegs enter needles. Initial infection occurs between late May and early Jul and coincides with budbreak and shoot elongation. Colonization within needles is exclusively intercellular and increases continuously ...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/07-170r1
更新日期：2008-05-01 00:00:00
abstract：:The morphology and phylogeny of Anthracoidea on Carex meadii (sect. Paniceae) collected in Illinois, Iowa, Wisconsin, USA, were studied by light microscopy, scanning electron microscopy and LSU rDNA sequence analyses. As a result A. caricis-meadii sp. nov. is described. The fungus differs morphologically from Anthraco...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/12-074
更新日期：2013-01-01 00:00:00
abstract：:The class Eurotiomycetes (Ascomycota, Pezizomycotina) is a monophyletic group comprising two major clades of very different ascomycetous fungi: (i) the subclass Eurotiomycetidae, a clade that contains most of the fungi previously recognized as Plectomycetes because of their mostly enclosed ascomata and prototunicate a...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/mycologia.98.6.1053
更新日期：2006-11-01 00:00:00
abstract：:Agaricus sect. Arvenses includes numerous species that are potential candidates for cultivation, and some have high nutritional and medicinal interests. Between 2012 and 2017, 147 specimens of A. sect. Arvenses were collected in China. For this study, nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), n...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.1080/00275514.2020.1830247
更新日期：2020-12-16 00:00:00
abstract：:Bupleurum gibraltarium is an evergreen shrub endemic to southern Spain and northern Algeria and Morocco. We have collected and cultured an undescribed Kabatiella species that is consistently associated with the flower rachises and leaves of B. gibraltarium in the province of Granada. The fungus forms melanized acervul...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/12-003
更新日期：2012-07-01 00:00:00
abstract：:The taxonomic position of Inocybe inexpectata is reconsidered on the basis of new molecular and morphological data from four published records. Up to three species can be recognized to which the name I. inexpectata has been applied. In addition to distinct morphological features present in this lineage it is proposed ...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/10-041
更新日期：2010-11-01 00:00:00
abstract：:Acremonium is one of the largest and most complex genera of hyphomycetes. Its taxonomy is not yet resolved and the phylogenetic value of the most distinctive morphological features is unknown. The species of this genus produce conidia in chains or form slimy heads or both. We have studied a set of clinical and environ...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/106.2.328
更新日期：2014-03-01 00:00:00
abstract：:Molecular and ultrastructural investigations are revealing unrealized diversity among chytrids as the taxonomic revision of order Chytridiales (Chytridiomycota) progresses. During a biodiversity survey of soil-inhabiting chytrids in Australia an undescribed chytrid was isolated from a soil sample collected in a cool t...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/08-194
更新日期：2009-09-01 00:00:00
abstract：:The host-endophyte interaction between roots of aspen (Populus tremuloides) and Cryptosporiopsis radicicola was examined primarily by transmission electron microscopy. Hyphae growing on the exterior of the inoculated roots had a thick, electron-dense, adhesive sheath. At hyphal contact and penetration, host epidermal ...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/08-134
更新日期：2009-03-01 00:00:00
abstract：:The genus Protocrea is redefined, based on holotype and fresh specimens of its type species P. farinosa, using morphology of teleomorph and anamorph and phylogenetic analyses of rpb2 sequences. Data based on currently available specimens suggest the existence of three well defined and three still unnamed species. Apar...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/08-101
更新日期：2008-11-01 00:00:00
abstract：:Two new species from the Russulaceae, Lactifluus aurantiotinctus and Russula pallidirosea, are described from American Samoa. Based on analyses of nuc rDNA internal transcribed spacer region barcodes (ITS), L. aurantiotinctus has an affinity to subgenus Lactariopsis and strong phylogeographic ties to Papua New Guinea....
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/15-171
更新日期：2016-03-01 00:00:00
abstract：:A new Rosellinia species, R. capetribulensis isolated from Calamus sp. in Australia is described. R. capetribulensis is characterized by perithecia immersed within a carbonaceous stroma surrounded by subiculum-like hyphae, asci with large, barrel-shaped amyloid apical apparatus and large dark brown spores. Morphologic...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/mycologia.97.5.1102
更新日期：2005-09-01 00:00:00
abstract：:The type of Scleroderma stellatum from Brazil exhibits a sharp echinulate, dark brown peridium, and the type of S. bermudense from Bermuda has a peridium that is loosely woven and fibrillose, whitish to pale brownish. These characters indicate two independent species. This information is contrary to that of Guzmán in ...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.1080/15572536.2005.11832886
更新日期：2004-11-01 00:00:00
abstract：:Hebeloma ifeleletorum is described as a new species from American Samoa. Based on analyses of ITS and combined nLSU-ITS datasets H. ifeleletorum clusters with but is distinct from described species that have been placed in the genus Anamika by some. The phylogenetic relationship of H. ifeleletorum to the genus Anamika...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/14-047
更新日期：2015-01-01 00:00:00
abstract：:We present a taxonomic and phylogenetic study of Puccinia species (rust fungi) infecting tribe Lycieae (Solanaceae), with focus on the New World taxa. Phylogenetic analyses using nuclear (nuc) rDNA 5.8S-ITS2 (ITS2) and mitochondrial (mt) cytochrome oxidase subunit 3 (CO3) show that Puccinia species occurring on Lyciae...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.1080/00275514.2018.1478538
更新日期：2018-07-01 00:00:00
abstract：:Sclerotinia sclerotiorum is a necrotrophic plant-pathogenic fungus that infects more than 400 species of plants. In this study the nascent polypeptide-associated complex α subunit gene of S. sclerotiorum (SsNACα; accession No. XP_001593856.1) was cloned and characterized. The relative transcript expression of SsNACα a...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/14-250
更新日期：2015-11-01 00:00:00
abstract：:A new species of Cortinarius, C. flavoaurantians sp. nov., is described from Italian Quercus woods based on both morphological and ITS rDNA data. This taxon is characterized by a yellowish pileus and cortina, a white universal veil and a pileipellis that reacts yellow-orange with KOH. Illustrations of the key micromor...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/12-072
更新日期：2012-11-01 00:00:00
abstract：:Seven species of bioluminescent fungi are recorded from the cloud forests in Mexico. Six represent new species belonging to the genus Mycena, whereas Mycena globulispora is a new distribution record for the country. Descriptions, illustrations, photographs, and an identification key to bioluminescent fungi species fro...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.1080/00275514.2018.1554172
更新日期：2019-03-01 00:00:00
abstract：:We reassessed the circumscription of the cantharelloid clade and identified monophyletic groups by using nLSU, nSSU, mtSSU and RPB2 sequence data. Results agreed with earlier studies that placed the genera Cantharellus, Craterellus, Hydnum, Clavulina, Membranomyces, Multiclavula, Sistotrema, Botryobasidium and the fam...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/mycologia.98.6.937
更新日期：2006-11-01 00:00:00
abstract：:Brevilegnia ensenadensis sp. nov. is described from litter (floating twigs, leaves, and roots) in a man-made, polluted channel near a petroleum refinery, in Buenos Aires Province, Argentina. The species is illustrated and compared with other species of the genus; it can be identified by the preponderance of smooth or ...
journal_title：Mycologia
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:A Tricladium anamorph for the discomycete Hymenoscyphus varicosporoides was established in culture from both conidia and ascospores collected in KhaoYai National Park, Thailand, and is compared with Tricladium indicum and T. marylandicum. Hymenoscyphus varicosporoides is compared with Cudoniella indica. ...
journal_title：Mycologia
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:The fungal genus Neurospora has a distinguished history as a laboratory model in genetics and biochemistry. The most recent milestone in this history has been the sequencing of the genome of the best known species, N. crassa. The hope and promise of a complete genome sequence is a full understanding of the biology of ...
journal_title：Mycologia
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Analysis of a worldwide collection of strains of Trichoderma asperellum sensu lato using multilocus genealogies of four genomic regions (tef1, rpb2, act, ITS1, 2 and 5.8s rRNA), sequence polymorphism-derived (SPD) markers, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) of t...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.3852/09-243
更新日期：2010-07-01 00:00:00
abstract：:Two species of Nitschkia are described from bark and wood of a legume shrub, native to the semiarid regions of Argentina. Nitschkia campylospora is characterized by asci with a variable number of ascospores, mainly 16, and curved ascospores; hairy ascomata and large ascospores are two distinct features of Nitschkia pi...
journal_title：Mycologia
pub_type： 杂志文章
doi：10.1080/15572536.2005.11832937
更新日期：2004-07-01 00:00:00
