Abstract：
:Cyanidin-3-O-β-glucoside (C3G) (CAS number 7084-24-4), a typical anthocyanin pigment that exists in the human diet, has been reported to have anti-inflammatory properties. However, the effect of C3G on lipopolysaccharide (LPS)-induced mastitis and the molecular mechanisms have not been investigated. In this study, we detected the protective effects of C3G on a LPS-induced mouse mastitis model and investigated the molecular mechanisms in LPS-stimulated mouse mammary epithelial cells (MMECs). Our results showed that C3G could attenuate mammary histopathologic changes and myeloperoxidase activity, and inhibit TNF-α, interleukin (IL)-1β, and IL-6 production caused by LPS. Meanwhile, C3G dose-dependently inhibited TNF-α and IL-6 in LPS-stimulated MMECs. C3G suppressed LPS-induced nuclear factor-κB (NF-κB) and interferon regulatory factor 3 (IRF3) activation. Furthermore, C3G disrupted the formation of lipid rafts by depleting cholesterol. Moreover, C3G activated liver X receptor (LXR)-ABCG1-dependent cholesterol efflux. Knockdown of LXRα abrogated the anti-inflammatory effects of C3G. In conclusion, C3G has a protective effect on LPS-induced mastitis. The promising anti-inflammatory mechanisms of C3G are associated with upregulation of the LXRα-ABCG1 pathway which result in disrupting lipid rafts by depleting cholesterol, thereby suppressing toll-like receptor 4-mediated NF-κB and IRF3 signaling pathways induced by LPS.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Fu Y,Wei Z,Zhou E,Zhang N,Yang Zdoi
10.1194/jlr.M047340subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1111-9issue
6eissn
0022-2275issn
1539-7262pii
jlr.M047340journal_volume
55pub_type
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journal_title：Journal of lipid research
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journal_title：Journal of lipid research
pub_type： 杂志文章
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更新日期：2001-08-01 00:00:00
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journal_title：Journal of lipid research
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pub_type： 杂志文章
doi：10.1194/jlr.D500005-JLR200
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1969-01-01 00:00:00
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journal_title：Journal of lipid research
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doi：
更新日期：1981-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1194/jlr.P081620
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1194/jlr.M800551-JLR200
更新日期：2009-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1194/jlr.M500532-JLR200
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
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journal_title：Journal of lipid research
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doi：
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doi：
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journal_title：Journal of lipid research
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doi：
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journal_title：Journal of lipid research
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doi：
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pub_type： 临床试验,杂志文章,多中心研究
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更新日期：2015-03-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
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journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
