Abstract:
:Genome-wide association studies (GWAS) have identified chromosomal loci that affect risk of coronary heart disease (CHD) independent of classical risk factors. One such association signal has been identified at 6q23.2 in both Caucasians and East Asians. The lead CHD-associated polymorphism in this region, rs12190287, resides in the 3' untranslated region (3'-UTR) of TCF21, a basic-helix-loop-helix transcription factor, and is predicted to alter the seed binding sequence for miR-224. Allelic imbalance studies in circulating leukocytes and human coronary artery smooth muscle cells (HCASMC) showed significant imbalance of the TCF21 transcript that correlated with genotype at rs12190287, consistent with this variant contributing to allele-specific expression differences. 3' UTR reporter gene transfection studies in HCASMC showed that the disease-associated C allele has reduced expression compared to the protective G allele. Kinetic analyses in vitro revealed faster RNA-RNA complex formation and greater binding of miR-224 with the TCF21 C allelic transcript. In addition, in vitro probing with Pb2+ and RNase T1 revealed structural differences between the TCF21 variants in proximity of the rs12190287 variant, which are predicted to provide greater access to the C allele for miR-224 binding. miR-224 and TCF21 expression levels were anti-correlated in HCASMC, and miR-224 modulates the transcriptional response of TCF21 to transforming growth factor-β (TGF-β) and platelet derived growth factor (PDGF) signaling in an allele-specific manner. Lastly, miR-224 and TCF21 were localized in human coronary artery lesions and anti-correlated during atherosclerosis. Together, these data suggest that miR-224 interaction with the TCF21 transcript contributes to allelic imbalance of this gene, thus partly explaining the genetic risk for coronary heart disease associated at 6q23.2. These studies implicating rs12190287 in the miRNA-dependent regulation of TCF21, in conjunction with previous studies showing that this variant modulates transcriptional regulation through activator protein 1 (AP-1), suggests a unique bimodal level of complexity previously unreported for disease-associated variants.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Miller CL,Haas U,Diaz R,Leeper NJ,Kundu RK,Patlolla B,Assimes TL,Kaiser FJ,Perisic L,Hedin U,Maegdefessel L,Schunkert H,Erdmann J,Quertermous T,Sczakiel Gdoi
10.1371/journal.pgen.1004263subject
Has Abstractpub_date
2014-03-27 00:00:00pages
e1004263issue
3eissn
1553-7390issn
1553-7404pii
PGENETICS-D-13-03233journal_volume
10pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::Genome reduction is typical of obligate symbionts. In cellular organelles, this reduction partly reflects transfer of ancestral bacterial genes to the host genome, but little is known about gene transfer in other obligate symbioses. Aphids harbor anciently acquired obligate mutualists, Buchnera aphidicola (Gammaproteo...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000827
更新日期:2010-02-26 00:00:00
abstract::Hereditary cystatin C amyloid angiopathy (HCCAA) is an autosomal dominant disease with high penetrance, manifest by brain hemorrhages in young normotensive adults. In Iceland, this condition is caused by the L68Q mutation in the cystatin C gene, with contemporary carriers reaching an average age of only 30 years. Here...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000099
更新日期:2008-06-20 00:00:00
abstract::ATM and ATR are two redundant checkpoint kinases essential for the stable maintenance of telomeres in eukaryotes. Previous studies have established that MRN (Mre11-Rad50-Nbs1) and ATRIP (ATR Interacting Protein) interact with ATM and ATR, respectively, and recruit their partner kinases to sites of DNA damage. Here, we...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000839
更新日期:2010-02-05 00:00:00
abstract::Genome-wide association studies (GWAS) have now been conducted for hundreds of phenotypes of relevance to human health. Many such GWAS involve multiple closely-related phenotypes collected on the same samples. However, the vast majority of these GWAS have been analyzed using simple univariate analyses, which consider ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008431
更新日期:2019-10-09 00:00:00
abstract::Iran, despite its size, geographic location and past cultural influence, has largely been a blind spot for human population genetic studies. With only sparse genetic information on the Iranian population available, we pursued its genome-wide and geographic characterization based on 1021 samples from eleven ethnic grou...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008385
更新日期:2019-09-24 00:00:00
abstract::Expansions of trinucleotide CAG/CTG repeats in somatic tissues are thought to contribute to ongoing disease progression through an affected individual's life with Huntington's disease or myotonic dystrophy. Broad ranges of repeat instability arise between individuals with expanded repeats, suggesting the existence of ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003280
更新日期:2013-01-01 00:00:00
abstract::Genome-wide transcription start site (TSS) profiles of the enterobacteria Escherichia coli and Klebsiella pneumoniae were experimentally determined through modified 5' RACE followed by deep sequencing of intact primary mRNA. This identified 3,746 and 3,143 TSSs for E. coli and K. pneumoniae, respectively. Experimental...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002867
更新日期:2012-01-01 00:00:00
abstract::Dominant mutations in the alpha-B crystallin (CryAB) gene are responsible for a number of inherited human disorders, including cardiomyopathy, skeletal muscle myopathy, and cataracts. The cellular mechanisms of disease pathology for these disorders are not well understood. Among recent advances is that the disease sta...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003544
更新日期:2013-06-01 00:00:00
abstract::Light and microRNAs (miRNAs) are key external and internal signals for plant development, respectively. However, the relationship between the light signaling and miRNA biogenesis pathways remains unknown. Here we found that miRNA processer proteins DCL1 and HYL1 interact with a basic helix-loop-helix (bHLH) transcript...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007247
更新日期:2018-03-09 00:00:00
abstract::In understanding the etiology of breast cancer, the contributions of both genetic and environmental risk factors are further complicated by the impact of breast developmental stage. Specifically, the time period ranging from childhood to young adulthood represents a critical developmental window in a woman's life when...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006261
更新日期:2016-08-18 00:00:00
abstract::Dynamic activity of signaling pathways, such as Notch, is vital to achieve correct development and homeostasis. However, most studies assess output many hours or days after initiation of signaling, once the outcome has been consolidated. Here we analyze genome-wide changes in transcript levels, binding of the Notch pa...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003162
更新日期:2013-01-01 00:00:00
abstract::Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both meiosis and mitosis, and a central regulator of their activity is the APC/C (Anaphase Promoting Complex/Cyclosome) that is especia...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002865
更新日期:2012-01-01 00:00:00
abstract::The unfolded protein response (UPR) is an adaptive signaling pathway utilized to sense and alleviate the stress of protein folding in the endoplasmic reticulum (ER). In mammals, the UPR is mediated through three proximal sensors PERK/PEK, IRE1, and ATF6. PERK/PEK is a protein kinase that phosphorylates the alpha subun...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0010037
更新日期:2005-09-01 00:00:00
abstract::The presence of 5-methylcytidine (m(5)C) in tRNA and rRNA molecules of a wide variety of organisms was first observed more than 40 years ago. However, detection of this modification was limited to specific, abundant, RNA species, due to the usage of low-throughput methods. To obtain a high resolution, systematic, and ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003602
更新日期:2013-06-01 00:00:00
abstract::Salt stress is an important environmental factor that significantly limits crop productivity worldwide. Studies on responses of plants to salt stress in recent years have identified novel signaling pathways and have been at the forefront of plant stress biology and plant biology in general. Thus far, research on salt ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003755
更新日期:2013-08-01 00:00:00
abstract::We have exploited the high selectivity of the homing endonuclease I-PpoI for the X-linked Anopheles gambiae 28S ribosomal genes to selectively target X chromosome carrying spermatozoa. Our data demonstrated that in heterozygous males, the expression of I-PpoI in the testes induced a strong bias toward Y chromosome-car...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000291
更新日期:2008-12-01 00:00:00
abstract::In plants, multiple detached tissues are capable of forming a pluripotent cell mass, termed callus, when cultured on media containing appropriate plant hormones. Recent studies demonstrated that callus resembles the root-tip meristem, even if it is derived from aerial organs. This finding improves our understanding of...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002911
更新日期:2012-08-01 00:00:00
abstract::Class-switch recombination (CSR), induced by activation-induced cytidine deaminase (AID), can be divided into two phases: DNA cleavage of the switch (S) regions and the joining of the cleaved ends of the different S regions. Here, we show that the DSIF complex (Spt4 and Spt5), a transcription elongation factor, is req...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002675
更新日期:2012-01-01 00:00:00
abstract::Cytotoxic T-lymphocyte associated protein 4 (CTLA4) is a negative regulator of T-cell proliferation. Polymorphisms in CTLA4 have been inconsistently associated with susceptibility to rheumatoid arthritis (RA) in populations of European ancestry but have not been examined in African Americans. The prevalence of RA in m...
journal_title:PLoS genetics
pub_type: 杂志文章,收录出版
doi:10.1371/journal.pgen.1000424
更新日期:2009-03-01 00:00:00
abstract::Interpreting the impact of human genome variation on phenotype is challenging. The functional effect of protein-coding variants is often predicted using sequence conservation and population frequency data, however other factors are likely relevant. We hypothesized that variants in protein post-translational modificati...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004919
更新日期:2015-01-22 00:00:00
abstract::Orofacial clefting is amongst the most common of birth defects, with both genetic and environmental components. Although numerous studies have been undertaken to investigate the complexities of the genetic etiology of this heterogeneous condition, this factor remains incompletely understood. Here, we describe mutation...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006470
更新日期:2017-01-12 00:00:00
abstract::Nodal and Activin are morphogens of the TGFbeta superfamily of signaling molecules that direct differential cell fate decisions in a dose- and distance-dependent manner. During early embryonic development the Nodal/Activin pathway is responsible for the specification of mesoderm, endoderm, node, and mesendoderm. In co...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002130
更新日期:2011-06-01 00:00:00
abstract::Topoisomerase 3α, a class I topoisomerase, consists of a TOPRIM domain, an active centre and a variable number of zinc-finger domains (ZFDs) at the C-terminus, in multicellular organisms. Whereas the functions of the TOPRIM domain and the active centre are known, the specific role of the ZFDs is still obscure. In cont...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007674
更新日期:2018-09-17 00:00:00
abstract::Hexavalent chromium [Cr(VI)] damages DNA and causes cancer, but it is unclear which DNA damage responses (DDRs) most critically protect cells from chromate toxicity. Here, genome-wide quantitative functional profiling, DDR measurements and genetic interaction assays in Schizosaccharomyces pombe reveal a chromate toxic...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007595
更新日期:2018-08-27 00:00:00
abstract::Type 2 diabetes (T2D) is more prevalent in African Americans than in Europeans. However, little is known about the genetic risk in African Americans despite the recent identification of more than 70 T2D loci primarily by genome-wide association studies (GWAS) in individuals of European ancestry. In order to investigat...
journal_title:PLoS genetics
pub_type: 杂志文章,meta分析
doi:10.1371/journal.pgen.1004517
更新日期:2014-08-07 00:00:00
abstract::Genome-wide association studies typically target inherited autosomal variants, but less studied genetic mechanisms can play a role in complex disease. Sex-linked variants aside, three genetic phenomena can induce differential risk in maternal versus paternal lineages of affected individuals: 1. maternal effects, refle...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004174
更新日期:2014-03-20 00:00:00
abstract::A variety of pathologies are associated with exposure to supraphysiological concentrations of essential metals and to non-essential metals and metalloids. The molecular mechanisms linking metal exposure to human pathologies have not been clearly defined. To address these gaps in our understanding of the molecular biol...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000053
更新日期:2008-04-25 00:00:00
abstract::There is increasing evidence that non-coding macroRNAs are major elements for silencing imprinted genes, but their mechanism of action is poorly understood. Within the imprinted Gnas cluster on mouse chromosome 2, Nespas is a paternally expressed macroRNA that arises from an imprinting control region and runs antisens...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001347
更新日期:2011-03-01 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pgen.1004496.]. ...
journal_title:PLoS genetics
pub_type: 已发布勘误
doi:10.1371/journal.pgen.1006491
更新日期:2016-12-05 00:00:00
abstract::The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems. In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-r...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008532
更新日期:2019-12-23 00:00:00