Abstract:
:We have exploited the high selectivity of the homing endonuclease I-PpoI for the X-linked Anopheles gambiae 28S ribosomal genes to selectively target X chromosome carrying spermatozoa. Our data demonstrated that in heterozygous males, the expression of I-PpoI in the testes induced a strong bias toward Y chromosome-carrying spermatozoa. Notably, these male mosquitoes also induced complete early dominant embryo lethality in crosses with wild-type females. Morphological and molecular data indicated that all spermatozoa, irrespectively of the inheritance of the transgene, carried a substantial amount of I-PpoI protein that could attack the maternally inherited chromosome X of the embryo. Besides the obvious implications for implementing vector control measures, our data demonstrated the feasibility of generating synthetic sex distorters and revealed the intriguing possibility of manipulating maternally inherited genes using wild-type sperm cells carrying engineered endonucleases.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Windbichler N,Papathanos PA,Crisanti Adoi
10.1371/journal.pgen.1000291subject
Has Abstractpub_date
2008-12-01 00:00:00pages
e1000291issue
12eissn
1553-7390issn
1553-7404journal_volume
4pub_type
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