Abstract:
:Genome reduction is typical of obligate symbionts. In cellular organelles, this reduction partly reflects transfer of ancestral bacterial genes to the host genome, but little is known about gene transfer in other obligate symbioses. Aphids harbor anciently acquired obligate mutualists, Buchnera aphidicola (Gammaproteobacteria), which have highly reduced genomes (420-650 kb), raising the possibility of gene transfer from ancestral Buchnera to the aphid genome. In addition, aphids often harbor other bacteria that also are potential sources of transferred genes. Previous limited sampling of genes expressed in bacteriocytes, the specialized cells that harbor Buchnera, revealed that aphids acquired at least two genes from bacteria. The newly sequenced genome of the pea aphid, Acyrthosiphon pisum, presents the first opportunity for a complete inventory of genes transferred from bacteria to the host genome in the context of an ancient obligate symbiosis. Computational screening of the entire A. pisum genome, followed by phylogenetic and experimental analyses, provided strong support for the transfer of 12 genes or gene fragments from bacteria to the aphid genome: three LD-carboxypeptidases (LdcA1, LdcA2,psiLdcA), five rare lipoprotein As (RlpA1-5), N-acetylmuramoyl-L-alanine amidase (AmiD), 1,4-beta-N-acetylmuramidase (bLys), DNA polymerase III alpha chain (psiDnaE), and ATP synthase delta chain (psiAtpH). Buchnera was the apparent source of two highly truncated pseudogenes (psiDnaE and psiAtpH). Most other transferred genes were closely related to genes from relatives of Wolbachia (Alphaproteobacteria). At least eight of the transferred genes (LdcA1, AmiD, RlpA1-5, bLys) appear to be functional, and expression of seven (LdcA1, AmiD, RlpA1-5) are highly upregulated in bacteriocytes. The LdcAs and RlpAs appear to have been duplicated after transfer. Our results excluded the hypothesis that genome reduction in Buchnera has been accompanied by gene transfer to the host nuclear genome, but suggest that aphids utilize a set of duplicated genes acquired from other bacteria in the context of the Buchnera-aphid mutualism.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Nikoh N,McCutcheon JP,Kudo T,Miyagishima SY,Moran NA,Nakabachi Adoi
10.1371/journal.pgen.1000827subject
Has Abstractpub_date
2010-02-26 00:00:00pages
e1000827issue
2eissn
1553-7390issn
1553-7404journal_volume
6pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::Plant stress responses require both protective measures that reduce or restore stress-inflicted damage to cellular structures and mechanisms that efficiently remove damaged and toxic macromolecules, such as misfolded and damaged proteins. We have recently reported that NBR1, the first identified plant autophagy adapto...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004116
更新日期:2014-01-30 00:00:00
abstract::Retinoblastoma (pRb) is a multifunctional regulator, which was likely present in the last common ancestor of all eukaryotes. The Arabidopsis pRb homolog RETINOBLASTOMA RELATED 1 (RBR1), similar to its animal counterparts, controls not only cell proliferation but is also implicated in developmental decisions, stress re...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007797
更新日期:2018-11-30 00:00:00
abstract::Gap junctions are present in both vertebrates and invertebrates from nematodes to mammals. Although the importance of gap junctions has been documented in many biological processes, the molecular mechanisms underlying gap junction dynamics remain unclear. Here, using the C. elegans PLM neurons as a model, we show that...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005948
更新日期:2016-03-25 00:00:00
abstract::Neural stem cell self-renewal, neurogenesis, and cell fate determination are processes that control the generation of specific classes of neurons at the correct place and time. The transcription factor Pax6 is essential for neural stem cell proliferation, multipotency, and neurogenesis in many regions of the central n...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000511
更新日期:2009-06-01 00:00:00
abstract::The number of recombination events per meiosis varies extensively among individuals. This recombination phenotype differs between female and male, and also among individuals of each gender. In this study, we used high-density SNP genotypes of over 2,300 individuals and their offspring in two datasets to characterize r...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000648
更新日期:2009-09-01 00:00:00
abstract::A variety of pathologies are associated with exposure to supraphysiological concentrations of essential metals and to non-essential metals and metalloids. The molecular mechanisms linking metal exposure to human pathologies have not been clearly defined. To address these gaps in our understanding of the molecular biol...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000053
更新日期:2008-04-25 00:00:00
abstract::The hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex. Several studies have proposed alternative interaction partners for beta-catenin or TCF, but the relevance of potential bifurcations in the distal Wnt pathway remains unclear. Here we study on a...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006700
更新日期:2017-04-03 00:00:00
abstract::This work analyses the genetic variation and evolutionary patterns of recessive resistance loci involved in matching-allele (MA) host-pathogen interactions, focusing on the pvr2 resistance gene to potyviruses of the wild pepper Capsicum annuum glabriusculum (chiltepin). Chiltepin grows in a variety of wild habitats in...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006214
更新日期:2016-08-04 00:00:00
abstract::Filamentous fungi produce diverse secondary metabolites (SMs) essential to their ecology and adaptation. Although each SM is typically produced by only a handful of species, global SM production is governed by widely conserved transcriptional regulators in conjunction with other cellular processes, such as development...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005096
更新日期:2015-03-18 00:00:00
abstract::Genetic background exerts a strong modulatory effect on the toxicity of aggregation-prone proteins in conformational diseases. In addition to influencing the misfolding and aggregation behavior of the mutant proteins, polymorphisms in putative modifier genes may affect the molecular processes leading to the disease ph...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000399
更新日期:2009-03-01 00:00:00
abstract::Although multiple environmental cues regulate the transition to flowering in Arabidopsis thaliana, previous studies have suggested that wild A. thaliana accessions fall primarily into two classes, distinguished by their requirement for vernalization (extended winter-like temperatures), which enables rapid flowering un...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0010006
更新日期:2005-07-01 00:00:00
abstract::Mammalian genomes harbor a larger than expected number of complex loci, in which multiple genes are coupled by shared transcribed regions in antisense orientation and/or by bidirectional core promoters. To determine the incidence, functional significance, and evolutionary context of mammalian complex loci, we identifi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020047
更新日期:2006-04-01 00:00:00
abstract::More than 5% of alternatively spliced internal exons in the human genome are derived from Alu elements in a process termed exonization. Alus are comprised of two homologous arms separated by an internal polypyrimidine tract (PPT). In most exonizations, splice sites are selected from within the same arm. We hypothesize...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000717
更新日期:2009-11-01 00:00:00
abstract::The cerebellum is a pivotal centre for the integration and processing of motor and sensory information. Its extended development into the postnatal period makes this structure vulnerable to a variety of pathologies, including neoplasia. These properties have prompted intensive investigations that reveal not only devel...
journal_title:PLoS genetics
pub_type: 杂志文章,评审
doi:10.1371/journal.pgen.1008630
更新日期:2020-04-16 00:00:00
abstract::Here we report a stop-mutation in the BOD1 (Biorientation Defective 1) gene, which co-segregates with intellectual disability in a large consanguineous family, where individuals that are homozygous for the mutation have no detectable BOD1 mRNA or protein. The BOD1 protein is required for proper chromosome segregation,...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006022
更新日期:2016-05-11 00:00:00
abstract::Phenylketonuria (PKU), one of the most common inherited diseases of amino acid metabolism, is caused by mutations in the phenylalanine hydroxylase (PAH) gene. Recently, PAH exon 11 was identified as a vulnerable exon due to a weak 3' splice site, with different exonic mutations affecting exon 11 splicing through disru...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007360
更新日期:2018-04-23 00:00:00
abstract::In the last two decades, mitochondrial DNA (mtDNA) and the non-recombining portion of the Y chromosome (NRY) have been extensively used in order to measure the maternally and paternally inherited genetic structure of human populations, and to infer sex-specific demography and history. Most studies converge towards the...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000200
更新日期:2008-09-26 00:00:00
abstract::The High Pathogenicity Island of Yersinia pseudotuberculosis IP32637 was previously shown to be horizontally transferable as part of a large chromosomal segment. We demonstrate here that at low temperature other chromosomal loci, as well as a non-mobilizable plasmid (pUC4K), are also transferable. This transfer, desig...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002529
更新日期:2012-01-01 00:00:00
abstract::We recently reported that centrosomal protein 164 (CEP164) regulates both cilia and the DNA damage response in the autosomal recessive polycystic kidney disease nephronophthisis. Here we examine the functional role of CEP164 in nephronophthisis-related ciliopathies and concomitant fibrosis. Live cell imaging of RPE-FU...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004594
更新日期:2014-10-23 00:00:00
abstract::Nonhomologous end-joining (NHEJ) is the primary DNA repair pathway thought to underlie chromosomal translocations and other genomic rearrangements in somatic cells. The canonical NHEJ pathway, including DNA ligase IV (Lig4), suppresses genomic instability and chromosomal translocations, leading to the notion that a po...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002080
更新日期:2011-06-01 00:00:00
abstract::Anti-tumor necrosis factor alpha (anti-TNF) biologic therapy is a widely used treatment for rheumatoid arthritis (RA). It is unknown why some RA patients fail to respond adequately to anti-TNF therapy, which limits the development of clinical biomarkers to predict response or new drugs to target refractory cases. To u...
journal_title:PLoS genetics
pub_type: 杂志文章,meta分析
doi:10.1371/journal.pgen.1003394
更新日期:2013-03-01 00:00:00
abstract::Although DNA methylation is the best characterized epigenetic mark, the mechanism by which it is targeted to specific regions in the genome remains unclear. Recent studies have revealed that local DNA methylation profiles might be dictated by cis-regulatory DNA sequences that mainly operate via DNA-binding factors. Co...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1009189
更新日期:2020-11-20 00:00:00
abstract::We carried out an admixture analysis of a sample comprising 1,019 individuals from all the provinces of Cuba. We used a panel of 128 autosomal Ancestry Informative Markers (AIMs) to estimate the admixture proportions. We also characterized a number of haplogroup diagnostic markers in the mtDNA and Y-chromosome in orde...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004488
更新日期:2014-07-24 00:00:00
abstract::The Rhox cluster on the mouse X chromosome contains reproduction-related homeobox genes expressed in a sexually dimorphic manner. We report that two members of the Rhox cluster, Rhox6 and 9, are regulated by de-methylation of histone H3 at lysine 27 by KDM6A, a histone demethylase with female-biased expression. Consis...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003489
更新日期:2013-05-01 00:00:00
abstract::Diphthamide is a highly modified histidine residue in eukaryal translation elongation factor 2 (eEF2) that is the target for irreversible ADP ribosylation by diphtheria toxin (DT). In Saccharomyces cerevisiae, the initial steps of diphthamide biosynthesis are well characterized and require the DPH1-DPH5 genes. However...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003334
更新日期:2013-01-01 00:00:00
abstract::Global climate change, increasingly erratic weather and a burgeoning global population are significant threats to the sustainability of future crop production. There is an urgent need for the development of robust measures that enable crops to withstand the uncertainty of climate change whilst still producing maximum ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005705
更新日期:2015-12-03 00:00:00
abstract::Autosomal dominant polycystic kidney disease (ADPKD) is characterized by formation of renal cysts that destroy the kidney. Mutations in PKD1 and PKD2, encoding polycystins-1 and -2, cause ADPKD. Polycystins are thought to function in primary cilia, but it is not well understood how these and other proteins are targete...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001361
更新日期:2011-04-01 00:00:00
abstract::Capsules allow bacteria to colonize novel environments, to withstand numerous stresses, and to resist antibiotics. Yet, even though genetic exchanges with other cells should be adaptive under such circumstances, it has been suggested that capsules lower the rates of homologous recombination and horizontal gene transfe...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007862
更新日期:2018-12-21 00:00:00
abstract::Recent comparative genomic analysis of alternative splicing has shown that protein modularity is an important criterion for functional alternative splicing events. Exons that are alternatively spliced in multiple organisms are much more likely to be an exact multiple of 3 nt in length, representing a class of "modular...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0010034
更新日期:2005-09-01 00:00:00
abstract::Marine ecosystems are changing rapidly as the oceans warm and become more acidic. The physical factors and the changes to ocean chemistry that they drive can all be measured with great precision. Changes in the biological composition of communities in different ocean regions are far more challenging to measure because...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007943
更新日期:2019-02-08 00:00:00