Abstract:
:The hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex. Several studies have proposed alternative interaction partners for beta-catenin or TCF, but the relevance of potential bifurcations in the distal Wnt pathway remains unclear. Here we study on a genome-wide scale the requirement for Armadillo (Arm, Drosophila beta-catenin) and Pangolin (Pan, Drosophila TCF) in the Wnt/Wingless(Wg)-induced transcriptional response of Drosophila Kc cells. Using somatic genetics, we demonstrate that both Arm and Pan are absolutely required for mediating activation and repression of target genes. Furthermore, by means of STARR-sequencing we identified Wnt/Wg-responsive enhancer elements and found that all responsive enhancers depend on Pan. Together, our results confirm the dogma of canonical Wnt/Wg signaling and argue against the existence of distal pathway branches in this system.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Franz A,Shlyueva D,Brunner E,Stark A,Basler Kdoi
10.1371/journal.pgen.1006700subject
Has Abstractpub_date
2017-04-03 00:00:00pages
e1006700issue
4eissn
1553-7390issn
1553-7404pii
PGENETICS-D-17-00130journal_volume
13pub_type
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