当前位置： SCI文献检索 > JOURNAL OF ETHNOPHARMACOLOGY期刊下所有文献 > The Operculina macrocarpa (l.) urb. (jalapa) tincture modulates human blood platelet aggregation.

The Operculina macrocarpa (l.) urb. (jalapa) tincture modulates human blood platelet aggregation.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Operculina macrocarpa is an ornamental climbing plant of the Northeastern Brazil extensively used in traditional medicine as depurative of the blood and for the treatment of thrombosis. To investigate the antiplatelet and anticoagulant potential of Operculina macrocarpa and to determine the possible mechanisms of action. MATERIAL AND METHODS:The Operculina macrocarpa tincture (OMT) was characterized by the polyphenol content and chromatographic profile established by HPLC with detection and quantification of three phenol acids (caffeic, clorogenic and gallic acids). The human platelet aggregation was induced in vitro by the agonists ADP, collagen, thrombin, epinephrine or arachidonic acid, and the antiplatelet effect of OMT was evaluated in the presence or absence of aspirin (a nonselective inhibitor of cyclooxygenase), pentoxifylline (a phosphodiesterase inhibitor), ticlopidine (a P2Y12 purinoceptor antagonist) or ODQ (a selective inhibitor of guanilate cyclase). The effect of OMT on the partial thromboplastin time, prothrombin time and bleeding time were investigated on human or rat plasma. RESULTS:The strongest antiplatelet effect of OMT (50-400 µg/mL) was observed on the ADP- induced aggregation with inhibitions up to 55%, while among others agonists (epinephrine, collagen, thrombin and arachidonic acid) maximal inhibitions reached by OMT (200 µg/mL) were on platelet aggregation induced by collagen (18%) or epinephrine (20%). The antiplatelet effect of OMT (400 µg/mL) was comparable to aspirin, a nonspecific inhibitor of cyclooxygenase. The ticlopidine and pentoxifylline increased 5.1 and 3.8 fold the inhibitory effect of OMT on ADP-induced platelet aggregation, respectively. On the other hand, l-arginine, ODQ and aspirin showed a slightly or no effect on antiplatelet effect of OMT. The bleeding time in rats was significantly increased by OMT, but the tincture did not interfere on the activated partial thromboplastin or prothrombin time in human plasma. CONCLUSIONS:This study showed that the tincture of Operculina macrocarpa has antiplatelet effect that cannot be attributed to a single biochemical mechanism and at least part of it cannot be related to the OMT inhibition of P2Y12 purinergic receptors.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Pierdoná TM,Lima NR,Rodrigues RC,Teixeira JP,Gonçalves RP,Fontenele JB,Vasconcelos SM,de Barros Viana GS,Leal LK

doi

10.1016/j.jep.2013.10.008

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

151-7

issue

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(13)00715-0

journal_volume

151

pub_type

杂志文章

在线工具

The Operculina macrocarpa (l.) urb. (jalapa) tincture modulates human blood platelet aggregation.