Abstract：
:Acetylcholinesterase (AChE) biosensor was developed through silica sol-gel (SiSG) immobilisation of AChE on the carbon paste electrode (CPE) and used as working electrode. AChE catalyses the cleavage of acetylthiocholine chloride (ASChCl or substrate) to thiocholine, which was oxidised to give a disulphide compound by dimerisation at 0.60V versus saturated calomel electrode. All the experiments were carried out in 0.1M phosphate buffer solution (PBS) at pH 7.0 and 0.1M KCl solution at room temperature. The limit of detection and limit of quantification values were found to be 0.058ppm, 0.044ppm and 0.194ppm, 0.147ppm for Malathion and Acephate, respectively. The response of the biosensor showed a good linearity range with an incubation time of 4min for Malathion and Acephate, respectively. This biosensor was used for the direct determination of pesticides without any pretreatment and it requires less time for analysis.
journal_name
Food Chemjournal_title
Food chemistryauthors
Raghu P,Madhusudana Reddy T,Reddaiah K,Kumara Swamy BE,Sreedhar Mdoi
10.1016/j.foodchem.2013.07.047subject
Has Abstractpub_date
2014-01-01 00:00:00pages
188-96eissn
0308-8146issn
1873-7072pii
S0308-8146(13)00975-8journal_volume
142pub_type
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