Abstract:
UNLABELLED:Clathrin-mediated endocytosis (CME) is conserved among eukaryotes and has been extensively analyzed at a molecular level. Here, we present an analysis of CME in the human fungal pathogen Candida albicans that shows the same modular structure as those in other fungi and mammalian cells. Intriguingly, C. albicans is perfectly viable in the absence of Arp2/3, an essential component of CME in other systems. In C. albicans, Arp2/3 function remains essential for CME as all 15 proteins tested that participate in CME, including clathrin, lose their characteristic dynamics observed in wild-type (WT) cells. However, since arp2/3 cells are still able to endocytose lipids and fluid-phase markers, but not the Ste2 and Mup1 plasma membrane proteins, there must be an alternate clathrin-independent pathway we term Arp2/3-independent endocytosis (AIE). Characterization of AIE shows that endocytosis in arp2 mutants relies on actin cables and other Arp2/3-independent actin structures, as inhibition of actin functions prevented cargo uptake in arp2/3 mutants. Transmission electron microscopy (TEM) showed that arp2/3 mutants still formed invaginating tubules, cell structures whose proper functions are believed to heavily rely on Arp2/3. Finally, Prk1 and Sjl2, two proteins involved in patch disassembly during CME, were not correctly localized to sites of endocytosis in arp2 mutants, implying a role of Arp2/3 in CME patch disassembly. Overall, C. albicans contains an alternative endocytic pathway (AIE) that relies on actin cable function to permit clathrin-independent endocytosis (CIE) and provides a system to further explore alternate endocytic routes that likely exist in fungal species. IMPORTANCE:There is a well-established process of endocytosis that is generally used by eukaryotic cells termed clathrin-mediated endocytosis (CME). Although the details are somewhat different between lower and higher eukaryotes, CME appears to be the dominant endocytic process in all eukaryotes. While fungi such as Saccharomyces cerevisiae have proven excellent models for dissecting the molecular details of endocytosis, loss of CME is so detrimental that it has been difficult to study alternate pathways functioning in its absence. Although the fungal pathogen Candida albicans has a CME pathway that functions similarly to that of S. cerevisiae, inactivation of this pathway does not compromise growth of yeast-form C. albicans. In these cells, lipids and fluid-phase molecules are still endocytosed in an actin-dependent manner, but membrane proteins are not. Thus, C. albicans provides a powerful model for the analysis of CME-independent endocytosis in lower eukaryotes.
journal_name
mBiojournal_title
mBioauthors
Epp E,Nazarova E,Regan H,Douglas LM,Konopka JB,Vogel J,Whiteway Mdoi
10.1128/mBio.00476-13subject
Has Abstractpub_date
2013-08-27 00:00:00pages
e00476-13issue
5issn
2150-7511pii
mBio.00476-13journal_volume
4pub_type
杂志文章相关文献
mBio文献大全abstract:UNLABELLED:The aerial surfaces of plants, or phyllosphere, are microbial habitats important to plant and human health. In order to accurately investigate microbial interactions in the phyllosphere under laboratory conditions, the composition of the phyllosphere microbiota should be representative of the diversity of mi...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01564-14
更新日期:2014-08-12 00:00:00
abstract::The effectiveness of influenza vaccines against circulating A(H1N1)pdm09 viruses was modest for several seasons despite the absence of antigenic drift of hemagglutinin (HA), the primary vaccine component. Since antibodies against HA and neuraminidase (NA) contribute independently to protection against disease, antigen...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00307-19
更新日期:2019-04-09 00:00:00
abstract:UNLABELLED:The nucleus has emerged as a key target for nucleomodulins, a family of effectors produced by bacterial pathogens to control host transcription or other nuclear processes. The virulence factor LntA from Listeria monocytogenes stimulates interferon responses during infection by inhibiting BAHD1, a nuclear pro...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00775-13
更新日期:2014-01-21 00:00:00
abstract::Aspergillus fumigatus is an opportunistic fungal pathogen that can cause life-threatening invasive lung infections in immunodeficient patients. The cellular and molecular processes of infection during onset, establishment, and progression of A. fumigatus infections are highly complex and depend on both fungal attribut...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02752-19
更新日期:2020-02-04 00:00:00
abstract::Ebola virus (EBOV) protein VP35 inhibits production of interferon alpha/beta (IFN) by blocking RIG-I-like receptor signaling pathways, thereby promoting virus replication and pathogenesis. A high-throughput screening assay, developed to identify compounds that either inhibit or bypass VP35 IFN-antagonist function, ide...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00368-17
更新日期:2017-04-04 00:00:00
abstract:UNLABELLED:Elevated intracellular levels of the bacterial second messenger c-di-GMP are known to suppress motility and promote sessility. Bacterial chemotaxis guides motile cells in gradients of attractants and repellents over broad concentration ranges, thus allowing bacteria to quickly adapt to changes in their surro...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00001-13
更新日期:2013-03-19 00:00:00
abstract::Low doses of antibiotics can trigger secondary metabolite biosynthesis in bacteria, but the underlying mechanisms are generally unknown. We sought to better understand this phenomenon by studying how the antibiotic trimethoprim activates the synthesis of the virulence factor malleilactone in Burkholderia thailandensis...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03210-19
更新日期:2020-02-25 00:00:00
abstract::Coccidioides posadasii is a pathogenic fungus that causes coccidioidomycosis in many arid regions of the Americas. One of these regions is bordered by the Caribbean Sea, and the surrounding landscape may play an important role in the dispersion of C. posadasii across South America through southeastern Mexico, Honduras...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01976-19
更新日期:2019-11-26 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma (KS), caused by KS-associated herpesvirus (KSHV), is the most common cancer among HIV-infected patients in Malawi and in the United States today. In Malawi, KSHV is endemic. We conducted a cross-sectional study of patients with HIV infection and KS with no history of chemo- or antiretroviral...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01633-14
更新日期:2014-09-23 00:00:00
abstract::Symbiotic microorganisms can have a profound impact on the host physiology and behavior, and novel relationships between symbionts and their hosts are continually discovered. A colony of social ants consists of various castes that exhibit distinct lifestyles and is, thus, a unique model for investigating how symbionts...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00408-20
更新日期:2020-04-21 00:00:00
abstract:UNLABELLED:No studies have examined the relationships between bacterial communities along sites of the upper aerodigestive tract of an individual subject. Our objective was to perform an intrasubject and intersite analysis to determine the contributions of two upper mucosal sites (mouth and nose) as source communities ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00037-15
更新日期:2015-03-03 00:00:00
abstract::Sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) functions as a unique redox-driven sodium pump, generating membrane potential, which is related to aminoglycoside antibiotic resistance. However, whether it modulates other metabolisms to confer antibiotic resistance is unknown. The present study showed that l...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02086-20
更新日期:2020-11-17 00:00:00
abstract::Membrane repair emerges as an innate defense protecting target cells against bacterial pore-forming toxins. Here, we report the first paradigm of Ca2+-dependent repair following attack by a small β-pore-forming toxin, namely, plasmid-encoded phobalysin of Photobacterium damselae subsp. damselae In striking contrast, V...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02083-16
更新日期:2017-02-14 00:00:00
abstract:UNLABELLED:Methicillin-resistant Staphylococcus aureus (MRSA) strains are leading causes of hospital-acquired infections in the United States, and clonal cluster 5 (CC5) is the predominant lineage responsible for these infections. Since 2002, there have been 12 cases of vancomycin-resistant S. aureus (VRSA) infection i...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00112-12
更新日期:2012-05-22 00:00:00
abstract::Plasmodium vivax causes heavy burdens of disease across malarious regions worldwide. Mature P. vivax asexual and transmissive gametocyte stages occur in the blood circulation, and it is often assumed that accumulation/sequestration in tissues is not an important phase in their development. Here, we present a systemati...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00625-18
更新日期:2018-05-08 00:00:00
abstract:UNLABELLED:Streptococcus pneumoniae (pneumococcus) is a frequent colonizer of the nasopharynx and one of the leading causative agents of otitis media, pneumonia, and meningitis. The current literature asserts that S. pneumoniae is transmitted person to person via respiratory droplets; however, environmental surfaces (f...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00092-11
更新日期:2011-05-24 00:00:00
abstract::The pharmacopeia used by physicians and laypeople in medieval Europe has largely been dismissed as placebo or superstition. While we now recognize that some of the materia medica used by medieval physicians could have had useful biological properties, research in this area is limited by the labor-intensive process of ...
journal_title:mBio
pub_type: 历史文章,杂志文章
doi:10.1128/mBio.03136-19
更新日期:2020-02-11 00:00:00
abstract::Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny. In contrast, adhesion and biofilm formation, which are potentially involved in gut colonization...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00729-20
更新日期:2020-06-23 00:00:00
abstract:UNLABELLED:The acquisition, delivery, and incorporation of metals into their respective metalloproteins are important cellular processes. These processes are tightly controlled in order to prevent exposure of cells to free-metal concentrations that could yield oxidative damage. Copper (Cu) is one such metal that is req...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00293-11
更新日期:2012-01-31 00:00:00
abstract:UNLABELLED:The current tuberculosis (TB) vaccine, Mycobacterium bovis Bacillus Calmette-Guérin (BCG), provides insufficient protection against pulmonary TB. Previously, we generated a listeriolysin-expressing recombinant BCG strain, which to date has successfully completed phase I and phase IIa clinical trials. In an a...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00679-16
更新日期:2016-05-24 00:00:00
abstract::The Pseudomonas quinolone signal (PQS) is an important quorum-sensing molecule in Pseudomonas aeruginosa that also mediates its own packaging and transport by stimulating outer membrane vesicle (OMV) formation. Because OMVs have been implicated in many virulence-associated behaviors, it is critical that we understand ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01034-17
更新日期:2017-08-08 00:00:00
abstract:UNLABELLED:The intracellular parasite Toxoplasma gondii infects a wide variety of vertebrate species globally. Infection in most hosts causes a lifelong chronic infection and generates immunological memory responses that protect the host against new infections. In regions where the organism is endemic, multiple exposur...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02280-14
更新日期:2015-02-24 00:00:00
abstract:UNLABELLED:Cholera continues to be a global threat, with high rates of morbidity and mortality. In 2011, a cholera outbreak occurred in Palawan, Philippines, affecting more than 500 people, and 20 individuals died. Vibrio cholerae O1 was confirmed as the etiological agent. Source attribution is critical in cholera outb...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00047-15
更新日期:2015-04-21 00:00:00
abstract::The Mo- and V-nitrogenases are two homologous members of the nitrogenase family that are distinguished mainly by the presence of different heterometals (Mo or V) at their respective cofactor sites (M- or V-cluster). However, the V-nitrogenase is ~600-fold more active than its Mo counterpart in reducing CO to hydrocarb...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00310-18
更新日期:2018-03-13 00:00:00
abstract::Commensal bacteria in the human nasal cavity are known to suppress opportunistic pathogen colonization by competing for limited space and nutrients. It has become increasingly apparent that some commensal bacteria also produce toxic compounds that directly inhibit or kill incoming competitors. Numerous studies suggest...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02491-18
更新日期:2019-01-08 00:00:00
abstract::Since the original report that Halomonas sp. strain GFAJ-1 was capable of using arsenic instead of phosphorus to sustain growth, additional studies have been conducted, and GFAJ-1 is now considered a highly arsenic-resistant but phosphorus-dependent bacterium. However, the mechanisms supporting the extreme arsenic res...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00515-18
更新日期:2018-05-01 00:00:00
abstract::In Escherichia coli, FtsEX coordinates peptidoglycan (PG) synthesis and hydrolysis at the septum. It acts on FtsA in the cytoplasm to promote recruitment of septal PG synthetases and recruits EnvC, an activator of septal PG hydrolases, in the periplasm. Following recruitment, ATP hydrolysis by FtsEX is thought to regu...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01247-20
更新日期:2020-07-07 00:00:00
abstract::Cystic fibrosis (CF) is caused by mutations in the CFTR gene and is associated with progressive and ultimately fatal infectious lung disease. There can be considerable variability in disease severity among individuals with the same CFTR mutations, and recent genome-wide association studies have identified secondary ge...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02073-17
更新日期:2017-12-19 00:00:00
abstract::The yeast Yarrowia lipolytica is a potent accumulator of lipids, and lipogenesis in this organism can be influenced by a variety of factors, such as genetics and environmental conditions. Using a multifactorial study, we elucidated the effects of both genetic and environmental factors on regulation of lipogenesis in Y...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00857-17
更新日期:2017-06-20 00:00:00
abstract::Glycoprotein B (gB), gD, and gH/gL constitute the fusion machinery of herpes simplex virus (HSV). Prior studies indicated that fusion occurs in a stepwise fashion whereby the gD/receptor complex activates the entire process, while gH/gL regulates the fusion reaction carried out by gB. Trimeric gB is a class III fusion...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00429-12
更新日期:2012-11-20 00:00:00