Crystals of threonyl-tRNA synthetase from Thermus thermophilus. Preliminary crystallographic data.

abstract：:Ricin is a potent plant toxin which acts by removing a specific adenine residue from the ribosome. The X-ray crystal structure of a new, tetragonal crystal form of the recombinant ricin A-chain diffracting to 1.8 A resolution has been determined via molecular replacement methods and refined to a crystallographic R-fac...

journal_title：Journal of molecular biology

authors： Weston SA,Tucker AD,Thatcher DR,Derbyshire DJ,Pauptit RA

更新日期：1994-12-09 00:00:00

abstract：:Recombination between RNAs associated with turnip crinkle virus is thought to occur during plus-strand synthesis at motifs resembling the 5'-ends of genomic, subgenomic and satellite RNAs. Common structural regions encompassing the motifs have been found for major crossover sites on two different minus-strand template...

journal_title：Journal of molecular biology

authors： Carpenter CD,Oh JW,Zhang C,Simon AE

更新日期：1995-02-03 00:00:00

abstract：:A protein is defined as an indexed string of elements at each level in the hierarchy of protein structure: sequence, secondary structure, super-secondary structure, etc. The elements, for example, residues or secondary structure segments such as helices or beta-strands, are associated with a series of properties and c...

journal_title：Journal of molecular biology

authors： Sali A,Blundell TL

更新日期：1990-03-20 00:00:00

abstract：:Nhp6p is an architectural Saccharomyces cerevisiae non-histone chromosomal protein that bends DNA and plays an important role in transcription and genome stability. We used the split-ubiquitin system to isolate proteins that interact with Nhp6p in vivo, and we confirmed 11 of these protein-protein interactions with gl...

journal_title：Journal of molecular biology

authors： Xue X,Lehming N

更新日期：2008-05-30 00:00:00

abstract：:Crystal structures of the enzyme creatine amidinohydrolase (creatinase, EC 3.5.3.3) with two different inhibitors, the reaction product sarcosine and the substrate creatine, bound have been analyzed by X-ray diffraction methods. With the inhibitor carbamoyl sarcosine, two different crystal forms at different pH values...

journal_title：Journal of molecular biology

authors： Coll M,Knof SH,Ohga Y,Messerschmidt A,Huber R,Moellering H,Rüssmann L,Schumacher G

更新日期：1990-07-20 00:00:00

abstract：:The SfiI endonuclease is a prototype for DNA looping. It binds two copies of its recognition sequence and, if Mg(2+) is present, cuts both concertedly. Looping was examined here on supercoiled and relaxed forms of a 5.5 kb plasmid with three SfiI sites: sites 1 and 2 were separated by 0.4 kb, and sites 2 and 3 by 2.0 ...

journal_title：Journal of molecular biology

authors： Embleton ML,Vologodskii AV,Halford SE

更新日期：2004-05-21 00:00:00

abstract：:High-density lipoproteins (HDLs) are complexes of proteins (mainly apoA-I and apoA-II) and lipids that remove cholesterol and prevent atherosclerosis. Understanding the distinct properties of the heterogeneous HDL population may aid the development of new diagnostic tools and therapies for atherosclerosis. Mature huma...

journal_title：Journal of molecular biology

authors： Gao X,Yuan S,Jayaraman S,Gursky O

更新日期：2009-04-03 00:00:00

abstract：:Nitrite reductase from Pseudomonas aeruginosa (EC 1.9.3.2), a redox enzyme synthesized by the bacterium grown in the presence of nitrate, is a soluble dimer of two identical subunits of 60 kDa, each containing one c and one d1 haem as prosthetic groups. A new crystal from of the Ps. aeruginosa nitrite reductase in the...

journal_title：Journal of molecular biology

authors： Tegoni M,Silvestrini MC,Lamzin VS,Brunori M,Cambillau C

更新日期：1994-10-21 00:00:00

abstract：:We describe a hidden Markov model, HMMSTR, for general protein sequence based on the I-sites library of sequence-structure motifs. Unlike the linear hidden Markov models used to model individual protein families, HMMSTR has a highly branched topology and captures recurrent local features of protein sequences and struc...

journal_title：Journal of molecular biology

authors： Bystroff C,Thorsson V,Baker D

更新日期：2000-08-04 00:00:00

abstract：:Network propagation is a powerful tool for genetic analysis which is widely used to identify genes and genetic modules that underlie a process of interest. Here we provide a graphical, web-based platform (http://anat.cs.tau.ac.il/WebPropagate/) in which researchers can easily apply variants of this method to data sets...

journal_title：Journal of molecular biology

authors： Biran H,Almozlino T,Kupiec M,Sharan R

更新日期：2018-07-20 00:00:00

abstract：:The feline immunodeficiency virus (FIV) is a lentivirus that is related to human immunodeficiency virus (HIV), causing a similar pathology in cats. It is a potential small animal model for AIDS and the FIV-based vectors are also being pursued for human gene therapy. Previous studies have mapped the FIV packaging signa...

journal_title：Journal of molecular biology

authors： Rizvi TA,Kenyon JC,Ali J,Aktar SJ,Phillip PS,Ghazawi A,Mustafa F,Lever AML

更新日期：2010-10-15 00:00:00

abstract：:We describe a new method for local protein structure prediction based on a library of short sequence pattern that correlate strongly with protein three-dimensional structural elements. The library was generated using an automated method for finding correlations between protein sequence and local structure, and contain...

journal_title：Journal of molecular biology

authors： Bystroff C,Baker D

更新日期：1998-08-21 00:00:00

abstract：:Tau displacement from microtubules is the first step in the onset of tauopathies and is followed by toxic protein aggregation. However, other non-canonical functions of Tau might have a role in these pathologies. Here, we demonstrate that a small amount of Tau localizes in the nuclear compartment and accumulates in bo...

journal_title：Journal of molecular biology

authors： Siano G,Varisco M,Caiazza MC,Quercioli V,Mainardi M,Ippolito C,Cattaneo A,Di Primio C

更新日期：2019-02-15 00:00:00

abstract：:Bacteriophage λ of Escherichia coli has two alternative life cycles after infection-host survival with lysogen formation, or host lysis and phage production. In a lysogen, CI represses the two lytic promoters, pR and pL, and activates its own transcription from the auto-regulated pRM promoter. During induction from th...

journal_title：Journal of molecular biology

authors： Lee S,Lewis DEA,Adhya S

更新日期：2018-01-05 00:00:00

abstract：:In vitro packaging of bacteriophage SPP1 DNA into procapsids is described and the requirements of this process were determined. Combination of proheads with an extract supplying terminase, DNA and phage tails yielded up to 10(7 )viable phages per milliliter of in vitro reaction under optimized conditions. The presence...

journal_title：Journal of molecular biology

authors： Dröge A,Tavares P

更新日期：2000-02-11 00:00:00

abstract：:The three-dimensional structure of barley malt alpha-amylase (isoform AMY2-2) was determined by multiple isomorphous replacement using three heavy-atom derivatives and solvent flattening. The model was refined using a combination of simulated annealing and conventional restrained least-squares crystallographic refinem...

journal_title：Journal of molecular biology

authors： Kadziola A,Abe J,Svensson B,Haser R

更新日期：1994-05-27 00:00:00

abstract：:The structure and activities of the recombination-promoting P22 Erf protein were examined in vitro. Treatment of the protein with elastase produces a stable amino-terminal fragment, consisting of amino acid residues 1 to (approximately) 136. We have purified this fragment, designated fragment B, to apparent homogeneit...

journal_title：Journal of molecular biology

authors： Poteete AR,Sauer RT,Hendrix RW

更新日期：1983-12-25 00:00:00

abstract：:We have investigated the structure of complexes formed between a series of poly(A)n (n = 30 to 480) and HD40 (helix-destabilizing protein, molecular weight of 40,000), the major protein component of 30 S heterogeneous nuclear ribonucleoprotein particles (hnRNP) from the brine shrimp Artemia salina. Protein HD40 is sim...

journal_title：Journal of molecular biology

authors： Thomas JO,Glowacka SK,Szer W

更新日期：1983-12-25 00:00:00

abstract：:The calpains are a family of cysteine proteases with closely related amino acid sequences, but a wide range of Ca(2+) requirements (K(d)). For m-calpain, K(d) is approximately 325microM, for mu-calpain it is approximately 50microM, and for calpain 3 it is not strictly known but may be approximately 0.1microM. On the b...

journal_title：Journal of molecular biology

authors： Hosfield CM,Elce JS,Jia Z

更新日期：2004-10-29 00:00:00

abstract：:The preprotein translocase of the inner mitochondrial membrane has only been described in Saccharomyces cerevisiae to date. We report that the essential subunit Tim17 is highly conserved in evolution. The targeting and assembly of yeast Tim17 as well as that of human and Drosophila melanogaster Tim17 were characterize...

journal_title：Journal of molecular biology

authors： Bömer U,Rassow J,Zufall N,Pfanner N,Meijer M,Maarse AC

更新日期：1996-10-04 00:00:00

abstract：:Genetic studies in Saccharomyces cerevisiae predict that the mismatch repair (MMR) factor MSH2-MSH3 binds and stabilizes branched recombination intermediates that form during single strand annealing and gene conversion. To test this model, we constructed a series of DNA substrates that are predicted to form during the...

journal_title：Journal of molecular biology

authors： Surtees JA,Alani E

更新日期：2006-07-14 00:00:00

abstract：:Genomic ORFans are orphan open reading frames (ORFs) with no significant sequence similarity to other ORFs. ORFans comprise 20-30% of the ORFs of most completely sequenced genomes. Because nothing can be learnt about ORFans via sequence homology, the functions and evolutionary origins of ORFans remain a mystery. Furth...

journal_title：Journal of molecular biology

authors： Siew N,Fischer D

更新日期：2004-09-10 00:00:00

abstract：:Solution NMR structures for sperm whale carbonmonoxy myoglobin have been calculated using 1301 distance restraints determined from nuclear Overhauser enhancement (NOE) measurements on 15N-labeled protein and chemical shift calculations for 385 protons. Starting structures included four crystal forms of myoglobin and 1...

journal_title：Journal of molecular biology

authors： Osapay K,Theriault Y,Wright PE,Case DA

更新日期：1994-11-25 00:00:00

abstract：:Although numerous measurements of amyloid assembly of different proteins under distinct conditions in vitro have been performed, the molecular mechanisms underlying the specific self-association of proteins into amyloid fibrils remain obscure. Elucidating the nature of the events that initiate amyloid formation remain...

journal_title：Journal of molecular biology

authors： Eichner T,Radford SE

更新日期：2009-03-13 00:00:00

abstract：:Single crystals of methanol dehydrogenase (MDH) from Methylobacterium extorquens AM1 have been grown by the vapour diffusion method. These crystals diffract to beyond 2 A resolution and are suitable for X-ray crystallography. They belong to the orthorhombic space group P2(1)2(1)2(1) and have the following unit cell pa...

journal_title：Journal of molecular biology

authors： Ghosh M,Harlos K,Blake CC,Richardson I,Anthony C

更新日期：1992-11-05 00:00:00

abstract：:To elucidate the general constraints imposed on the structure of the D and T-loops in functional tRNAs, active suppressor tRNAs were selected in vivo from a combinatorial tRNA gene library in which several nucleotide positions in these loops were randomized. Analysis of the nucleotide sequences of the selected clones ...

journal_title：Journal of molecular biology

authors： Doyon FR,Zagryadskaya EI,Chen J,Steinberg SV

更新日期：2004-10-08 00:00:00

abstract：:Previous work used hydrogen exchange (HX) experiments in kinetic and equilibrium modes to study the reversible unfolding and refolding of cytochrome c (Cyt c) under native conditions. Accumulated results now show that Cyt c is composed of five individually cooperative folding units, called foldons, which unfold and re...

journal_title：Journal of molecular biology

authors： Krishna MM,Maity H,Rumbley JN,Lin Y,Englander SW

更新日期：2006-06-23 00:00:00

abstract：:The thermal melting of a dicyclic 29-residue peptide, having helix-stabilizing side-chain to side-chain covalent links at each terminal, has been studied by circular dichroism spectropolarimetry (CD) and differential scanning calorimetry (DSC). The CD spectra for this dicyclic peptide indicate that it is monomeric, al...

journal_title：Journal of molecular biology

authors： Taylor JW,Greenfield NJ,Wu B,Privalov PL

更新日期：1999-08-27 00:00:00

abstract：:FF domains are small protein-protein interaction modules that have two flanking conserved phenylalanine residues. They are present in proteins involved in transcription, RNA splicing, and signal transduction, and often exist in tandem arrays. Although several individual FF domain structures have been determined by NMR...

journal_title：Journal of molecular biology

authors： Lu M,Yang J,Ren Z,Sabui S,Espejo A,Bedford MT,Jacobson RH,Jeruzalmi D,McMurray JS,Chen X

更新日期：2009-10-23 00:00:00

abstract：:Fasciculin 2 and toxin alpha proteins belong to the same structural family of three-fingered snake toxins. They act on different targets, but in each case the binding region involves residues from loops I and II. The superimposition of the two structures suggests that these functional regions correspond to structurall...

journal_title：Journal of molecular biology

authors： Le Du MH,Ricciardi A,Khayati M,Ménez R,Boulain JC,Ménez A,Ducancel F

更新日期：2000-03-03 00:00:00