Abstract:
AIM:To determine whether chemokines such as SDF-1 and monocyte chemoattractant protein-1 (MCP-1) are responsible for hydrogen peroxide (H2 O2 )-induced extracellular matrix (ECM) degradation and to identify the underlying mechanism in human dental pulp cells (HDPCs). METHOD:Human dental pulp cells were exposed to 0.4 mmol H2 O2 for 48 h. mRNA expression and protein expression were examined by RT-PCR and Western blot analysis, respectively. The mRNA expression of chemokine (SDF-1 and MCP-1), their receptors (CXCR4 and CXCR2) and extracellular matrix proteins was evaluated by reverse transcriptase-polymerase chain reaction. The production of SDF-1, MCP-1, CXCR4 and CCR2 in the culture medium was determined by enzyme-linked immunosorbent assay. Signal transduction pathway was examined by Western blotting. RESULTS:Hydrogen peroxide provoked the activation of MCP-1 and SDF-1 mRNA and their respective receptors, CXCR4 and CXCR2. H2 O2 treatment concomitantly downregulated the expression of ECM molecules, such as type I collagen, elastin and fibronectin, and upregulated the mRNA expression of matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-8 and MMP-9. Hydrogen peroxide-induced ECM degradation and MMP upregulation were blocked by neutralizing antibodies and siRNAs directed against SDF-1 and MCP-1. Inhibition of SDF-1 and MCP-1 blocked the H2 O2 -induced activation of Akt, p38, ERK and NF-kB. CONCLUSION:Inhibition of SDF and MCP-1 is a potent component of reducing release reactive oxygen species-induced ECM degradation in HDPCs and may play an important role in pulpal and periapical inflammation.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Kim DS,Kang SI,Lee SY,Noh KT,Kim ECdoi
10.1111/iej.12147subject
Has Abstractpub_date
2014-03-01 00:00:00pages
298-308issue
3eissn
0143-2885issn
1365-2591journal_volume
47pub_type
杂志文章abstract::Following extraction of a symptomatic mandibular premolar which had been subjected to two periradicular surgical procedures, significant apical healing was identified histologically. The implications of these findings are discussed in relation to contemporary advocated treatment regimens, case assessment, and interpre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00280.x
更新日期:1994-11-01 00:00:00
abstract:AIM:To report the management of an iatrogenic incident in which an endodontic file was swallowed accidentally and passed into the gastrointestinal tract. SUMMARY:A 51-year-old male swallowed a 21 mm, size 30 endodontic file during root canal treatment without rubber dam. In the absence of clinical complications, the p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01392.x
更新日期:2008-07-01 00:00:00
abstract:AIM:To determine whether the outcome of cone beam computed tomography (CBCT) examinations performed in accordance with the European Commission guidelines in a clinical setting has an impact on choosing diagnoses in endodontics. METHODOLOGY:A prospective observational study was conducted. Fifty-three consecutive patien...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12350
更新日期:2015-06-01 00:00:00
abstract:AIM:To evaluate the neurotoxic effects of two endodontic sealers, AH-26 and Roth 801, on firing excitability and action potential configuration of F1 neural cells in the suboesophageal ganglia of Helix aspersa. METHODOLOGY:A conventional intracellular current clamp technique was used to study the blocking effects of A...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2003.00751.x
更新日期:2003-12-01 00:00:00
abstract:AIM:To evaluate the fracture resistance and fracture patterns of root filled maxillary first premolar teeth (with mesio-occlusal cavity preparation) restored with several composite restoration designs. METHODOLOGY:One hundred extracted sound human maxillary first premolars were randomly divided into five groups. Group...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2011.02005.x
更新日期:2012-06-01 00:00:00
abstract:AIM:This study was designed to examine whether enamel matrix derivative (EMD) could induce reparative dentine formation without eliciting adverse side-effects in pulpotomized teeth in the miniature swine. METHODOLOGY:Pulpotomy was performed in 36 mandibular incisor teeth from 11 adult miniature swine. Following the su...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00556.x
更新日期:2002-05-01 00:00:00
abstract:AIM:To introduce and assess a novel method for measuring washout resistance of cement-based dental materials, including mineral trioxide aggregate (MTA), to qualitatively verify the results with a clinical simulation and to evaluate the washout resistance of a new root-end filling material. METHODOLOGY:A method for as...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02108.x
更新日期:2013-02-01 00:00:00
abstract:AIM:To examine the clinical and radiographic appearance of teeth that suffered premature interruption of root development and were treated by an mineral trioxide aggregate (MTA) apical plug technique. SUMMARY:Eleven teeth with immature root apices in 11 patients were treated nonsurgically by the manual application of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01240.x
更新日期:2007-06-01 00:00:00
abstract:AIM:To investigate the smallest dentine thickness in mesial canals of mandibular molars along the cervical and middle thirds of the root by means of a micro-computed tomographic (micro-CT) technology and digital image analysis. METHODOLOGY:Fifty mesial roots of mandibular molars having two independent canals (mesiobuc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13141
更新日期:2019-10-01 00:00:00
abstract::The reliability of two commonly used methods of sealability testing, dye leakage under normal atmospheric pressure and dye leakage in a partial vacuum, was tested using a repeated studies design. Forty extracted teeth were instrumented and obturated using lateral condensation of gutta-percha and Sealapex sealer. One-h...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00291.x
更新日期:1995-05-01 00:00:00
abstract::The aim of this systematic review was to address the question: Do different irrigating protocols have an impact on the dislocation resistance of mineral trioxide aggregate (MTA)-based materials? The review was performed using a well-defined search strategy in three databases (PubMed, Scopus, Web of Science) to include...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12898
更新日期:2018-08-01 00:00:00
abstract:AIM:To investigate an immortalized murine odontoblast cell line as a potential alternative for experimental studies on dentinogenesis. METHODOLOGY:The MO6-G3 cell line was investigated morphologically over 3, 7, 11 and 42 days of culture, using histochemical localization of dentine sialoprotein (DSP), alkaline phospha...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01089.x
更新日期:2006-06-01 00:00:00
abstract:AIMS:To evaluate the microbiota of endodontic infections in deciduous teeth by Checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). METHODOLOGY:Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01805.x
更新日期:2011-03-01 00:00:00
abstract:AIM:This randomized clinical trial evaluated clinical sign/symptoms as well as histological pulp reactions in terms of inflammation and mineralized bridge formation after partial pulpotomy of sound human premolars and placement of a bioceramic paste (iRoot BP) or tooth-colored ProRoot MTA as pulp-covering biomaterials....
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.12231
更新日期:2014-09-01 00:00:00
abstract:AIM:To determine whether nisin, a bacteriocin, would be effective at killing Enterococcus faecalis and Streptococcus gordonii cells in solution and within the root canal system. METHODOLOGY:Bacterial isolates of E. faecalis and S. gordonii were grown from glycerol stocks in closed tubes containing BHY broth at 37 degr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00846.x
更新日期:2004-10-01 00:00:00
abstract::The short-term tissue responses to two potential root-end filling materials, a light-cured glass ionomer cement (Vitrebond) and a reinforced zinc oxide-eugenol cement (Kalzinol), were compared with that to amalgam using a previously devised experimental model. In 24 premolar teeth of beagle dogs (47 roots), a collecti...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00077.x
更新日期:1997-07-01 00:00:00
abstract:AIM:To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2 ], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and R...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13403
更新日期:2021-01-01 00:00:00
abstract:AIM:To compare the efficacy of reciprocating and rotary techniques with that of hand files for removing gutta-percha and sealer from root canals. METHODOLOGY:The root canals of fifty-four human extracted maxillary central incisors were cleaned and shaped using a crown-down technique to a size 40 and filled with gutta-...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12085
更新日期:2013-10-01 00:00:00
abstract:AIM:To evaluate the effect of three nickel-titanium (Ni-Ti) rotary systems with varying tapers on stress distribution and to analyse potential fracture patterns as well as the volume of fracture-susceptible regions in two-rooted maxillary premolars. METHODOLOGY:The root canals of three single-rooted premolars were pre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12770
更新日期:2018-05-01 00:00:00
abstract:AIM:To compare periapical health using samples from prehistoric and historic periods until the present day, and to emphasize the major risk indicators for apical periodontitis (AP). METHODOLOGY:A comparative survey to assess periapical health was performed on five samples (525 individuals) drawn from different periods...
journal_title:International endodontic journal
pub_type: 历史文章,杂志文章
doi:10.1111/j.1365-2591.2005.00905.x
更新日期:2005-05-01 00:00:00
abstract:AIM:To elucidate the expressions of MMP-8 and MMP-13 in experimentally induced rat periradicular lesions by means of the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining. METHODOLOGY:Thirty rats were used and periradicular lesions in mandibular first molar teeth were establishe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01880.x
更新日期:2011-08-01 00:00:00
abstract:AIM:To develop a suitable protocol for screening extracted teeth so as to create anatomically well-balanced experimental groups for endodontic studies. METHODOLOGY:Sixty pairs (120 teeth) of contralateral premolars (CLPs) were collected. The evaluation of samples for anatomical features was performed in the following ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12553
更新日期:2016-10-01 00:00:00
abstract:AIM:To perform multiparametric analysis of the effects of soya milk (SM), whole milk (WM) and Hank's balanced salt solution (HBSS) on the viability of fibroblasts (HGF). The study also aimed to evaluate the influence of these solutions on bovine root dentine according to OH- and PO43- on the surface. METHODOLOGY:The H...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12739
更新日期:2017-12-01 00:00:00
abstract:AIM:To evaluate the impact of activated platelets on the mitogenic expansion of human dental pulp-derived cells (DPC) in vitro. METHODOLOGY:The effect of supernatants released from activated platelets (PRS) and the corresponding platelet membranes (MEM) on proliferation and protein synthesis of DPC was evaluated. The ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01650.x
更新日期:2010-02-01 00:00:00
abstract::The aim of designing and fabricating the surgical templates was to assist the surgical component of premolar transplantation, by establishing and replicating the root dimensions of the donor premolar tooth. The correct template could be used to assess the socket preparation (width and depth) prior to placement of the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02077.x
更新日期:2012-11-01 00:00:00
abstract:AIM:To describe combined endodontic, surgical and orthodontic treatment of a maxillary lateral incisor fused with a supernumerary. SUMMARY:Double tooth is a dental irregularity consequent to fusion of two or more teeth or dental gemination. The teeth most commonly involved are deciduous, but in 0.1% of cases permanent...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01391.x
更新日期:2008-06-01 00:00:00
abstract:AIM:To investigate the composition of materials and leachate of a hydrated prototype cement composed of tricalcium silicate and radiopacifier and compare this to other tricalcium silicate-based cements (Biodentine and Bioaggregate) to assess whether the additives in the proprietary brand cements affect the hydration of...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12039
更新日期:2013-07-01 00:00:00
abstract:AIM:To conduct three-dimensional micro-CT analysis of bone destruction, periapical sclerotic changes and inflammatory root resorption (IRR) to compare the differences between Enterococcus faecalis (Ef)- and Porphyromonas gingivalis (Pg)-induced chronic apical periodontitis (CAP). METHODOLOGY:Mono-species bacteria-indu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13095
更新日期:2019-07-01 00:00:00
abstract:AIM:To compare 2D with 3D radiography in assessing the treatment outcome 1 year after periapical surgery. METHODOLOGY:In this prospective study, periapical radiographs (PA) and cone beam computed tomography (CBCT) were performed 1 year after periapical surgery. Three calibrated observers independently evaluated the ra...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12548
更新日期:2016-10-01 00:00:00
abstract:AIM:To evaluate the effect of repeated sterilization cycles in dry oven or autoclave, on the mechanical behaviour and fatigue resistance of rotary endodontic Ni-Ti instruments. METHODOLOGY:New Ni-Ti instruments were subjected to five consecutive sterilization cycles in a dry oven or steam autoclave. Microhardness was ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01138.x
更新日期:2006-09-01 00:00:00