Abstract:
PURPOSE:We will test the hypothesis that ultrasound supported by polymerase chain reaction (PCR) could improve bacterial identification in non-infected prosthetic joint loosening. The aim was to detect bacterial species in non-infected prosthetic joint loosening using ultrasound and 16S rRNA gene sequencing. METHODS:A total of 16 patients (11 women and five men) aged 46-80 years (mean age 65.7) with diagnosed knee or hip implant loosening (mean implant survival of 102.1 months) were investigated. Bacterial culture and DNA sequencing were used to detect bacteria on the surface of failed implants removed during revision arthroplasty. The results of pre- and intraoperative culture and DNA sequencing were compared. Histopathological analysis was also performed. RESULTS:The number of positive cultures rises with a higher level of C-reactive protein (CRP). The results of the cultures from synovial fluid obtained through joint aspiration were consistent with sonicates from components of prostheses in 12 cases (75%). Bacterial DNA was found in 90% of patients with negative synovial fluid culture. PCR revealed two or more bacterial species, often of the same genus: Ralstonia pickettii, Pseudomonas spp., Brevibacterium spp., Lactobacillus spp., Propionibacterium spp. and Staphylococcus spp.These are micro-organisms present in the environment or on the human body and often associated with compromised immunity. CONCLUSIONS:The ultrasound procedure followed by PCR and sequencing improve bacterial identification in silent prosthetic joint infection. The lack of clinical signs of infection and negative preoperative and intraoperative cultures do not exclude the presence of micro-organisms on the implants.
journal_name
Int Orthopjournal_title
International orthopaedicsauthors
Bereza PL,Ekiel A,Auguściak-Duma A,Aptekorz M,Wilk I,Kusz DJ,Wojciechowski P,Martirosian Gdoi
10.1007/s00264-013-1955-9subject
Has Abstractpub_date
2013-10-01 00:00:00pages
2037-43issue
10eissn
0341-2695issn
1432-5195journal_volume
37pub_type
杂志文章abstract:PURPOSE:Our aim was to investigate whether serum and synovial-fluid (SF) concentrations of interleukin-6 (IL-6), leptin, adiponectin, resistin or visfatin are associated with joint pain in hip and knee in end-stage osteoarthritis (OA). METHODS:A cross-sectional study assessing patients with hip and knee OA undergoing ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2416-9
更新日期:2014-12-01 00:00:00
abstract:PURPOSES:Acetabular fractures are more and more common in the elderly. Open reduction and internal fixation (ORIF) may lead to poor outcomes and high revision rates. Primary total hip arthroplasty (THA) combined with internal fixation, also known as the combined hip procedure (CHP), associated with dual mobility cup (D...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04757-w
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:The objective of this study was to analyze the prevalence and causes of early re-operation after hip replacement surgery using short bone-preserving stems in a large multicentre series. Specifically, we evaluated the clinical features of periprosthetic fractures occurring around short stems. METHODS:A total...
journal_title:International orthopaedics
pub_type: 杂志文章,多中心研究
doi:10.1007/s00264-018-3930-y
更新日期:2018-09-01 00:00:00
abstract:PURPOSE:Management of the unexplained, painful large diameter metal-on-metal (MOM) hip replacement is difficult. Although there are guidelines for surgeons, there is no clear documented evidence describing the overall threshold for revision surgery. The 2010 product recall of the DePuy Articular Surface Replacement (AS...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2369-z
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:A posterior-anterior vertebral vector is proposed to facilitate visualization and understanding of scoliosis. The aim of this study was to highlight the interest of using vertebral vectors, especially in the horizontal plane, in clinical practice. METHODS:We used an EOS two-/three-dimensional (2D/3D) system an...
journal_title:International orthopaedics
pub_type: 杂志文章,评审
doi:10.1007/s00264-017-3548-5
更新日期:2017-11-01 00:00:00
abstract:PURPOSE:Hamstring tendon grafts are a popular choice for anterior cruciate ligament (ACL) reconstruction. Typical morbidity reported after hamstring tendon harvest is muscle weakness in flexion, and whilst still a matter for debate, this morbidity may be greater if the gracilis tendon is harvested in addition to the se...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-012-1672-9
更新日期:2013-02-01 00:00:00
abstract:PURPOSE:The aim of this study was to compare total knee arthroplasty (TKA) procedures between different countries with regard to epidemiological data and surgical technique by reference to the worldwide arthroplasty registers. METHODS:A systematic search was carried out using the EFORT website to identify the relevant...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-013-1933-2
更新日期:2013-08-01 00:00:00
abstract::First metatarsophalangeal joint replacement is an alternative surgical procedure to arthrodesis in the treatment of moderate-to-severe hallux rigidus. However, few studies have been published about functional outcome after joint reconstructive procedures for hallux rigidus. The purpose of this study was to assess clin...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-009-0935-6
更新日期:2010-10-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-003-0427-z
更新日期:2003-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章,评审
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2391-1
更新日期:2014-09-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04928-9
更新日期:2021-01-09 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00264-014-2571-z
更新日期:2015-05-01 00:00:00
abstract::Results of two methods, conventional open reduction-internal plating and minimally invasive plating osteosynthesis (MIPO), in the treatment of mid-distal humeral shaft fractures were compared. Thirty-three patients were retrospectively analysed and divided into two groups. Group A (n = 17) patients were treated by MIP...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-009-0753-x
更新日期:2010-02-01 00:00:00
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journal_title:International orthopaedics
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更新日期:2020-09-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-013-2194-9
更新日期:2014-05-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-013-2123-y
更新日期:2014-01-01 00:00:00
abstract::A description is given of the vertical and horizontal components of the forces applied to the floor by the foot in walking. Information about the characteristics of the normal force pattern was obtained from the analysis of the gait of 214 normal subjects (108 young adults, 63 elderly people and 43 children). It is su...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00266460
更新日期:1983-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00178753
更新日期:1990-01-01 00:00:00
abstract::We have successfully treated a middle aged woman with long-standing posterior dislocations of the knee, using two stage external fixation and arthrodesis. ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640050259
更新日期:1998-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-015-2740-8
更新日期:2015-06-01 00:00:00
abstract::Thirty-seven femoral shaft fractures occurred in 2360 cemented hip arthroplasties carried out between 1971 and 1989 at varying intervals after operation (average 3.7 years). Three types of fracture were recognised and treatment depended on the type, as did the prognosis. Good results were obtained in 70% of the cases....
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00182995
更新日期:1992-01-01 00:00:00
abstract::The common peroneal nerves of 15 male rats were severed and the rats were divided into four groups: immediate grafting (I group); grafting delayed one month (IM group); grafting delayed for three months (3M group) and 5 healthy rats were used as controls (control group). All the rats were sacrificed 5 months after gra...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00266515
更新日期:1985-01-01 00:00:00
abstract::Forty patients with unilateral osteoarthritis of the hip were studied with dual energy X-ray absorptiometry to quantify disuse osteopenia in their affected leg by examining the proximal femur and tibia. Bone loss was assessed as a percentage of the contralateral value which compares bone mineral density of the affecte...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00188325
更新日期:1994-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04696-6
更新日期:2020-10-01 00:00:00
abstract::The articular cartilage and synovial membrane of immature and mature chicken knee joints were studied by light, scanning and transmission microscopy. The findings differed from human articular cartilage and we conclude that the chicken knee joint is not suitable as a model for human joint degeneration. ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00183553
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:The purpose of this study was to find a suitable method of labelling cartilage samples for the measurement of distraction distances in biomechanical testing. METHODS:Samples of bovine cartilage were labelled using five different methods: hydroquinone and silver nitrate (AgNO3), potassium permanganate (KMnO4) w...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-011-1468-3
更新日期:2012-07-01 00:00:00
abstract::Septic and aseptic loosening with or without extensive bone loss after total knee replacement are the most common indications for knee fusion. Both external fixation and intramedullary nailing can be used for the treatment, though the latter appears to be the method of choice for most patients. Nine patients were trea...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-006-0129-4
更新日期:2006-12-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-018-3758-5
更新日期:2018-08-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04623-9
更新日期:2020-10-01 00:00:00