Abstract:
:Oxidative stress in plants causes ferredoxin down-regulation and NADP(+) shortage, over-reduction of the photosynthetic electron transport chain, electron leakage to oxygen and generation of reactive oxygen species (ROS). Expression of cyanobacterial flavodoxin in tobacco chloroplasts compensates for ferredoxin decline and restores electron delivery to productive routes, resulting in enhanced stress tolerance. We have designed an in vivo system to optimize flavodoxin reduction and NADP(+) regeneration under stress using a version of cyanobacterial ferredoxin-NADP(+) reductase without the thylakoid-binding domain. Co-expression of the two soluble flavoproteins in the chloroplast stroma resulted in lines displaying maximal tolerance to redox-cycling oxidants, lower damage and decreased ROS accumulation. The results underscore the importance of chloroplast redox homeostasis in plants exposed to adverse conditions, and provide a tool to improve crop tolerance toward environmental hardships.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Giró M,Ceccoli RD,Poli HO,Carrillo N,Lodeyro AFdoi
10.1016/j.fob.2011.10.004subject
Has Abstractpub_date
2011-11-11 00:00:00pages
7-13issn
2211-5463pii
FOB4journal_volume
1pub_type
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