Abstract:
:Sorafenib, the first-line agent for treatment of advanced hepatocellular carcinoma (HCC), improves median overall survival by approximately 3 months. In the present study, we investigated whether sorafenib combined with cucurbitacin B (CuB), a natural tetracyclic triterpenoid isolated from Cucurbitaceae, exerts enhanced antitumor effects against HCC. Cell viability and colony formation ability were detected by cell-counting kit-8 and colony formation assays. Cell cycle and apoptosis were analyzed by flow cytometry. Protein expression was detected by western blotting. HepG2 xenografts in nude mice were used to evaluate in vivo antitumor effects. We report that sorafenib and CuB exhibited synergistic effects on cellular proliferation inhibition and cell apoptosis induction, but not on cell cycle arrest. Furthermore, combination treatment enhanced levels of cleaved caspase 3 and cleaved caspase 9, but suppressed phosphorylation of STAT3. Epidermal growth factor, a potent stimulator of signal transducer and activator of transcription-3 (STAT3), promoted cell viability and colony formation ability, whereas combination treatment exerted inhibitory effects on epidermal growth factor-induced STAT3 phosphorylation. Finally, HepG2 xenograft mice cotreated with sorafenib and CuB exhibited reduced tumor progression without notable weight loss. In conclusion, sorafenib and CuB exert synergistic antitumor effects through a pathway that may involve STAT3 phosphorylation, and this may represent a promising therapeutic approach for treatment of HCC.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Wang X,Li H,Li D,Bai Y,Zhang Y,Yan X,Li J,Zhao R,Liu J,Liu W,Shi M,Xu C,Yang T,Zhang Tdoi
10.1002/2211-5463.13035subject
Has Abstractpub_date
2020-11-11 00:00:00issn
2211-5463pub_type
杂志文章相关文献
FEBS Open Bio文献大全abstract::l-Ornithine cyclodeaminase (OCD) is involved in l-proline biosynthesis and catalyzes the unique deaminating cyclization of l-ornithine to l-proline via a Δ(1)-pyrroline-2-carboxyrate (Pyr2C) intermediate. Although this pathway functions in only a few bacteria, many archaea possess OCD-like genes (proteins), among whic...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.07.005
更新日期:2014-07-10 00:00:00
abstract::Recent evidence has shown that miR-409-3p was down-regulated in several types of cancer, including osteosarcoma. However, the potential role of miR-409-3p in osteosarcoma remains largely unknown. In the present study, we showed that overexpression of miR-409-3p in osteosarcoma cells inhibited cell proliferation in vit...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12177
更新日期:2017-01-27 00:00:00
abstract::DNA damage-inducible 1 (Ddi1) is a multidomain protein with one of the domains being retropepsin-like. HIV-1 protease inhibitors were found to reduce opportunistic infections caused by pathogens like Leishmania and Plasmodium, and some of them were shown to inhibit the growth of these parasites. In Leishmania, Ddi1 wa...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12491
更新日期:2018-08-03 00:00:00
abstract::Hirschmanniella mucronata is a plant-parasitic nematode that is widespread in rice production areas and causes 10-25% yield losses a year on average. Here, we investigated the mechanism of resistance to this nematode by comparing the transcriptomes of roots from resistant (Jiabali) and susceptible (Bawangbian) varieti...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12737
更新日期:2019-11-01 00:00:00
abstract::Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12811
更新日期:2020-05-01 00:00:00
abstract::The Wilms tumor protein 1 (WT1) transcription factor has been associated in malignant melanoma with cell survival and metastasis, thus emerging as a candidate for targeted therapy. A lysine-arginine rich peptide, WT1-pTj, derived from the ZF domain of WT1 was evaluated as an antitumor agent against A2058 human melanom...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.01.007
更新日期:2014-01-21 00:00:00
abstract::Shiga toxin (Stx) causes fatal systemic complications. Stx induces apoptosis, but the mechanism of which is unclear. We report that Stx induced rapid reduction of short-lived anti-apoptotic proteins followed by activation of caspase 9 and the progression of apoptosis. Proteasome inhibitors prevented the reduction of a...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.06.005
更新日期:2015-06-12 00:00:00
abstract::The gene encoding MG Orn has been identified from a metagenomic library created from the intertidal zone in Svalbard and encodes a protein of 184 amino acid residues. The mg orn gene has been cloned, recombinantly expressed in Escherichia coli, and purified to homogeneity. Biochemical characterization of the enzyme sh...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12720
更新日期:2019-10-01 00:00:00
abstract::Immunoglobulin (Ig) A nephropathy (IgAN) is the most common glomerulonephritis, which is characterized by the deposition of IgA antibody in the glomerulus. Systematic dissection of immune composition may contribute to a better understanding of the alternations in the immune system in IgAN. To this end, here we applied...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13006
更新日期:2020-12-01 00:00:00
abstract::Glioma is one of the most common types of malignant primary central nervous system tumor, and prognosis for this disease is poor. As autophagic drugs have been reported to induce glioma cell death, we investigated the potential prognostic role of autophagy-associated long non-coding RNA (lncRNA) in glioma patients. In...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12601
更新日期:2019-03-05 00:00:00
abstract::The Masculinizer (Masc) gene encodes a CCCH-tandem zinc finger protein that controls both masculinization and dosage compensation in the silkworm Bombyx mori. We previously measured the masculinizing activity of the lepidopteran Masc proteins using B. mori ovary-derived cell line BmN-4. Here, we established an RNA-seq...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12698
更新日期:2019-09-01 00:00:00
abstract::Fibroblast growth factor 11 (FGF11) is a member of the intracellular fibroblast growth factor superfamily. Here, we identified FGF11 as a novel mediator of adipogenesis. During 3T3-L1 adipocyte differentiation, the expression of FGF11 decreased at the mitotic clonal expansion stage and increased at the terminal differ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12619
更新日期:2019-03-12 00:00:00
abstract::Rheumatoid arthritis (RA) is a chronic inflammatory disease with increased risk of cardiovascular events and mortality that can be attributed to accelerated atherosclerosis. This pilot study aimed to investigate if changes in blood parameters were compatible with atherosclerotic events in RA patients. To this aim, 45 ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12531
更新日期:2018-11-06 00:00:00
abstract::Oxidative stress in plants causes ferredoxin down-regulation and NADP(+) shortage, over-reduction of the photosynthetic electron transport chain, electron leakage to oxygen and generation of reactive oxygen species (ROS). Expression of cyanobacterial flavodoxin in tobacco chloroplasts compensates for ferredoxin declin...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2011.10.004
更新日期:2011-11-11 00:00:00
abstract::Angiotensin converting enzyme (ACE) is a zinc-dependent dipeptidyl carboxypeptidase with an essential role in blood pressure homeostasis in mammals. ACE has long been targeted in the treatment of hypertension through ACE inhibitors, however current inhibitors are known to cause severe side effects. Therefore, there is...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.08.004
更新日期:2015-08-11 00:00:00
abstract::Cisplatin-based chemotherapy is the standard treatment of choice for head and neck squamous cell carcinoma (HNSCC). The efficiency of platinum-based therapies is directly influenced by the development of tumor resistance. Multiple signaling pathways have been linked to tumor resistance, including activation of nuclear...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.12.003
更新日期:2013-12-30 00:00:00
abstract::Peroxisome proliferator-activated receptor-gamma coactivator-1β (PGC-1β) is a transcriptional regulator whose increased expression activates energy expenditure-related genes in skeletal muscles. However, how PGC-1β is regulated remains largely unclear. Here, we show that PGC-1β gene expression is negatively correlated...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12898
更新日期:2020-07-01 00:00:00
abstract::Hypoxia-inducible factor (HIF) is a crucial regulator of cellular and systemic responses to low oxygen levels. Here we firstly cloned three HIF-α isoforms from the basal branches of Osteichthyes and used computational tools to characterise the molecular change underlying the functional divergence of HIF-α isoforms in ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.09.004
更新日期:2013-09-19 00:00:00
abstract::In this study, we showed that cyanocobalamin dodecylamine, a ribose 5'-carbamate derivative of cyanocobalamin, was absorbed and accumulated to significant levels by Caenorhabditis elegans and was not further metabolized. The levels of methylmalonic acid and homocysteine, which serve as indicators of cobalamin deficien...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.07.008
更新日期:2014-08-01 00:00:00
abstract::Microorganisms or LPS (lipopolysaccharide), an outer membrane component of Gram-negative bacteria, can induce a systemic inflammatory response that leads to sepsis, multiple organ dysfunction, and mortality. Here, we investigated the role of cyclophilin D (CypD)-dependent mitochondrial permeability transition (mPT) in...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13091
更新日期:2021-01-20 00:00:00
abstract::Niclosamide is a potent inhibitor of osteoclastogenesis and bone remodeling. DK-520 is an acyl derivative of Niclosamide and significantly increased both the plasma concentration and the duration of exposure of Niclosamide when dosed orally. However, at present the effect of DK-520 on osteoclastogenesis has not been r...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12921
更新日期:2020-08-01 00:00:00
abstract::Osteosarcoma is the most common primary solid malignancy of the bone, and its high mortality usually correlates with early metastasis. In this study, we developed a risk score model to help predict metastasis at the time of diagnosis. We downloaded and mined four expression profile datasets associated with osteosarcom...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12592
更新日期:2019-02-02 00:00:00
abstract::Previous studies have proposed that the human papillomavirus (HPV) E6 oncoproteins modify the transcriptional activity of eIF4E through mechanisms dependent on p53 degradation. However, the effect of these oncoproteins on pathways regulating the activity of the eIF4E protein remains poorly understood. Hence, we invest...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12987
更新日期:2020-12-01 00:00:00
abstract::RAC3 is a coactivator of glucocorticoid receptor and nuclear factor-κB (NF-κB) that is usually over-expressed in tumors and which also has important functions in the immune system. We investigated the role of the inflammatory response in the control of RAC3 expression levels in vivo and in vitro. We found that inflamm...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.04.009
更新日期:2014-05-02 00:00:00
abstract::Prostate cancer (PCa) is the most frequently diagnosed male cancer. An earlier study of a cohort of 333 primary prostate carcinomas showed that 74% of these tumors fell into one of seven subtypes of a molecular taxonomy defined by specific gene fusions (ERG, ETV1/4 and FLI1) or mutations (SPOP, FOXA1 and IDH1). Molecu...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13004
更新日期:2020-12-01 00:00:00
abstract::The base excision repair pathway plays an important role in correcting damage induced by either physiological or external effects. This repair pathway removes incorrect bases from the DNA. The uracil base is among the most frequently occurring erroneous bases in DNA, and is cut out from the phosphodiester backbone via...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12567
更新日期:2019-02-09 00:00:00
abstract::Human melanoma is a highly malignant tumor originating from cutaneous melanocytes. The noncoding RNA microRNA (miR)-21-5p has been reported to be expressed at high levels in malignant melanocytic skin tissues, but its potential functional role in melanoma remains poorly understood. Here, we explored the cellular effec...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12819
更新日期:2020-05-01 00:00:00
abstract::Neointimal hyperplasia (NIH) is a complicated inflammatory process contributing to vascular restenosis. The present study aimed to explore whether chemokine-like factor 1 (CKLF1) aggravates NIH via the nuclear factor-kappa B (NF-κB)/vascular cell adhesion molecule-1 (VCAM-1) pathway. We found the expression of CKLF1 a...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12942
更新日期:2020-09-01 00:00:00
abstract::Due to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In contrast to mammals,...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12727
更新日期:2019-10-01 00:00:00
abstract::Human cytomegalovirus (HCMV) infection can be accelerated by intracellular and extracellular hydrogen peroxide (H2O2) stimulation, mediated by the activation of the p38 mitogen-activated protein kinase (MAPK) pathway. However, it remains unknown whether host gene expression is involved in H2O2-upregulated HCMV replica...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12105
更新日期:2016-08-15 00:00:00