Abstract:
PURPOSE:This study investigated which of the reciprocal stimuli between articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) played the more important role in enhancing cartilage matrix formation, and examined the relative importance of physical contact and soluble factors in the co-culture system. METHODS:Rat ACs and bone marrow MSCs with green fluorescent protein (GFP-BMSCs) were co-cultured in vitro with or without direct cell-cell contact at the ratio of 2:1. After co-culturing in direct cell-cell contact, ACs and GFP-BMSCs were separated by flow cytometry. The effects of different co-culture methods were analysed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. RESULTS:SOX-9, COL2 and aggrecan mRNA levels and protein expression in ACs co-cultured with direct cell-cell contact were significantly higher than in ACs co-cultured without direct cell-cell contact; and similar results were found in GFP-BMSCs. After co-culture either with or without direct cell-cell contact, mRNA levels and protein expression of SOX-9, COL2 and aggrecan in GFP-BMSCs were significantly lower than in ACs in the equivalent co-culture systems. Though the expression of chondrocyte-specific proteins in GFP-BMSCs was enhanced, the protein expression was still much lower than in ACs cultured alone. CONCLUSIONS:Reciprocal interactions exist between ACs and BMSCs in co-culture. The stimulating and supporting effects of BMSCs on ACs were more important in enhancing cartilage-matrix formation than the reciprocal effect of ACs on BMSCs. Both soluble factors and direct physical contact occur in AC/BMSC co-cultures, with physical contact playing a predominant, or at least very important role.
journal_name
Int Orthopjournal_title
International orthopaedicsauthors
Zuo Q,Cui W,Liu F,Wang Q,Chen Z,Fan Wdoi
10.1007/s00264-013-1782-zsubject
Has Abstractpub_date
2013-04-01 00:00:00pages
747-52issue
4eissn
0341-2695issn
1432-5195journal_volume
37pub_type
杂志文章abstract:PURPOSE:The purpose of this systematic review and meta-analysis of randomised controlled trials is to assess the effectiveness of no drainage when compared to drainage in total knee arthroplasty, in terms of recovery of knee flexion, reduction in swelling, length of hospital stay and haemoglobin levels following TKA. ...
journal_title:International orthopaedics
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00264-014-2455-2
更新日期:2015-04-01 00:00:00
abstract::From October 2005 to September 2006, 20 patients with an acute thoracolumbar burst fracture underwent posterior instrumentation with transpedicular intracorporeal calcium sulphate grafting. The mean age of the patients at surgery was 41.8 years; 14 were men and six were women. The mean follow-up period was 28.5 months...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-008-0677-x
更新日期:2009-12-01 00:00:00
abstract:PURPOSE:Although previously frozen shoulder was thought to resolve by two to three years, recent studies demonstrated the symptoms can remain for much longer. Manipulation under anaesthetic (MUA) has been shown to be successful in relieving pain and restoring function. Yet, concerns have been raised regarding its safet...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-017-3558-3
更新日期:2018-02-01 00:00:00
abstract:PURPOSE:Femoroacetabular impingement is a new disease concept for hip disorders in young adults suggested as a major cause of primary hip osteoarthritis in Western countries. However, significant controversy exists regarding the prevalence and contribution of impingement deformities to osteoarthritis in Japan, owing to...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2393-z
更新日期:2014-08-01 00:00:00
abstract::We carried out a retrospective review of 32 consecutive patients (30 adults and two children) with total or partial lesions of the brachial plexus who had surgical repair using nerve grafting, neurotisation, and neurolysis between January 1991 and December 2003. The outcome measures of muscular strength were correlate...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-005-0017-3
更新日期:2005-12-01 00:00:00
abstract:PURPOSE:This systematic literature review analysed the change in range of knee flexion from pre-operative values, following conventional posterior stabilised (PS) and high-flexion (H-F) PS total knee arthroplasty (TKA). METHODS:We calculated the weighted mean differences of pre- and postoperative flexion using meta-an...
journal_title:International orthopaedics
pub_type: 杂志文章,meta分析
doi:10.1007/s00264-011-1228-4
更新日期:2011-09-01 00:00:00
abstract::Pyogenic spondylitis is a neurological and life threatening condition. It encompasses a broad range of clinical entities, including pyogenic spondylodiscitis, septic discitis, vertebral osteomyelitis, and epidural abscess. The incidence though low appears to be on the rise. The diagnosis is based on clinical, radiolog...
journal_title:International orthopaedics
pub_type: 杂志文章,评审
doi:10.1007/s00264-011-1384-6
更新日期:2012-02-01 00:00:00
abstract:PURPOSE:Although unilateral total knee arthroplasty (TKA) is successful in restoring function, there are concerns in relation to asymmetric gait patterns. Bi-cruciate retaining (BCR) TKA design preserves both anterior and posterior cruciate ligaments with the potential to restore normal joint kinematics. The purpose of...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-018-3986-8
更新日期:2018-12-01 00:00:00
abstract::We have studied the incidence of deep wound infection after hip fracture surgery and its relationship to urinary catheterisation. Infection after hemiarthroplasty occurred in 16/949 cases and 10/1,143 sliding hip screw fixations. No cases of deep sepsis were seen for the 203 patients treated with an intramedullary nai...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-006-0227-3
更新日期:2007-08-01 00:00:00
abstract::The laminae were removed from the lower lumbar spines of five cadavers within four hours of death. A short length of rubber tube was inserted between the nerve roots and the lumbar disc and the tension monitored using semiconductor pressure transducers. The angulatory stresses exerted on the lumbar nerve roots during ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00265716
更新日期:1979-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-013-2194-9
更新日期:2014-05-01 00:00:00
abstract:INTRODUCTION:The aim of paper is to check how many preliminary reports (PR) reach a long-term publication (LT), and analyse differences in outcomes and complications between them. METHODS:A literature search was performed to identify shoulder preliminary or early reports. Fifty one papers fulfilled criteria and were c...
journal_title:International orthopaedics
pub_type: 杂志文章,评审
doi:10.1007/s00264-016-3228-x
更新日期:2017-05-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-018-3758-5
更新日期:2018-08-01 00:00:00
abstract::The original publication of this paper contain an error for the first name and last name of the second author have been mixed up as "Wenliang, Z.". ...
journal_title:International orthopaedics
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00264-018-3948-1
更新日期:2018-07-01 00:00:00
abstract::Forty patients with unilateral osteoarthritis of the hip were studied with dual energy X-ray absorptiometry to quantify disuse osteopenia in their affected leg by examining the proximal femur and tibia. Bone loss was assessed as a percentage of the contralateral value which compares bone mineral density of the affecte...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00188325
更新日期:1994-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-012-1549-y
更新日期:2013-03-01 00:00:00
abstract::The patella clunk syndrome is not an uncommon complication, particularly after posterior-stabilized (PS) total knee arthroplasty. The present study was a consecutive unselected series comparing the incidence of the patella clunk syndrome amongst two comparable groups of elderly patients with knee arthritis implanted w...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-001-0316-2
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:Degenerative cervical spondylolisthesis has received insufficient attention, in contrast to degenerative lumbar spondylolisthesis. In fact, degenerative cervical spondylolisthesis may be more common than previously thought. METHODS:In order to provide appropriate guidelines for the treatment of degenerative ce...
journal_title:International orthopaedics
pub_type: 杂志文章,评审
doi:10.1007/s00264-010-1203-5
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Insufficient levels of serum 25-hydroxyvitamin D (25-OHD) influence the knee joint cartilage and lead to development and progression of knee osteoarthritis (OA). The purpose of this study was to determine the status of serum 25-OHD levels in patients with knee OA compared with controls. METHODS:A total of 1...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-010-1186-2
更新日期:2011-11-01 00:00:00
abstract::Over the last two centuries, treatment of hip pathology has evolved from joint excision and osteotomies to modern total hip arthroplasty (THA), which is considered one of the most successful surgical interventions ever developed. We here review the history of hip procedures that preceded Themistocles Glück. This evalu...
journal_title:International orthopaedics
pub_type: 传,历史文章,杂志文章
doi:10.1007/s00264-013-2004-4
更新日期:2013-11-01 00:00:00
abstract::We treated 19 patients with piriformis muscle syndrome. All patients complained of pain in the buttocks and the posterior thigh. Clinically the buttock was tender and passive stretching increased the pain. In ten patients skin sensation was reduced and three had a limp. All patients were treated with tenotomy. At foll...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-001-0319-z
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:We reviewed data to determine outcomes for 21 consecutive Vancouver type B1 or type C periprosthetic fractures that we treated between 2001 and 2008 using a nickel-titanium shape-memory sawtooth-arm embracing fixator. METHODS:The study participants were 12 men and 9 women (mean age, 70.8 years; range, 42-85 ye...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-011-1325-4
更新日期:2012-03-01 00:00:00
abstract:PURPOSE:The aim of this study was to map the strength distribution of the human patella and correlate it to the subchondral bone plate density obtained by means of computed tomographyosteoabsorptiometry (CT-OAM). METHODS:Measurements were performed at 34 standardized points on each patella. The mineralization patterns...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-012-1545-2
更新日期:2012-09-01 00:00:00
abstract::After division of the tendo achilles in rats heterotopic bone forms in the muscle and in the tendon stumps. Tenotomy was performed in 26 male rats. Thirteen received Verapamil (36 mm/Kg) for six days per week for five months, and the remainder were not given this drug. The onset of heterotopic ossification was monitor...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00182655
更新日期:1990-01-01 00:00:00
abstract::Old age is frequently associated with a poorer functional outcome after THA. This might be based upon muscular damage resulting from surgical trauma. Minimally invasive approaches have been widely promoted on the basis of the muscle sparing effect. The aim of the study was to evaluate of the functional outcome and the...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-010-1166-6
更新日期:2011-02-01 00:00:00
abstract::Loss of joint space, formation of osteophytes and deformation are common features of osteoarthritis. Little information exists about the radiological features of arthrosis in relation to clinical findings and the radiological appearance in degenerative shoulder joint disease especially with regard to decision making a...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-009-0846-6
更新日期:2010-06-01 00:00:00
abstract::In 16 patients with ulnar wrist pain, we performed primary arthroscopic or open repair of the peripheral rim tears of the triangular fibrocartilage complex (TFCC) (14 ulnar, 4 volar, and 3 radial tears). The wrist function was assessed before and 1 year after the repair using the Mayo-modified wrist score. The average...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640100285
更新日期:2001-01-01 00:00:00
abstract::Between 1993 and 1998, 49 consecutive patients with impending or complete pathological fractures of the femur due to metastatic bone disease were treated with intramedullary nailing. Twenty-four were treated with a long Gamma nail and 32 with an AO nail with a cephalomedullary spiral blade. Both implants gave a good f...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640000114
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to assess the anatomical results, functional results and the incidence of arthritis of a mixed intra- and extra-articular hamstring tendon based reconstruction for anterior instability of the knee. METHODS:This retrospective study consists of 100 knees in 99 patients (22 female and 77...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-013-1957-7
更新日期:2013-08-01 00:00:00
abstract::A retrospective study evaluating complications in 2,233 consecutive patients of subaxial cervical disorders treated with an anterior cervical locking plate was performed, and recommendations for prevention and treatment were made. The average length of follow-up was 1.3 years. Any loosening or breaking of the plates a...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-007-0369-y
更新日期:2008-10-01 00:00:00