Clinical feasibility of a novel biphasic osteochondral composite for matrix-associated autologous chondrocyte implantation.


OBJECTIVE:Matrix-associated autologous chondrocyte implantation has been used to treat cartilage defects. We developed a biphasic cylindrical osteochondral composite construct for such use, and conducted this study to determine its feasibility for treating osteochondral lesions in human knees. METHOD:Ten patients with symptomatic osteochondral lesions at femoral condyles were treated by replacing pathological tissue with the construct of dl-poly-lactide-co-glycolide, whose lower body was impregnated with β-tricalcium phosphate and served as osseous phase. The construct had a chamber to load double-minced autologous cartilage, serving as source of chondrocytes. Osteochondral lesion was drill-fashioned a pit of identical dimension as the construct. Chondrocyte-laden construct was press-fit to fill the pit. Postoperative outcome was evaluated using Knee Injury and Osteoarthritis Outcome Score (KOOS) scale up to 24 months. Magnetic resonance image was taken, and sample tissue was collected with second-look arthroscopic needle biopsy at 12 months. Outcome parameters were primarily safety of surgery, and secondarily postoperative change in KOOS and regeneration of hyaline cartilage and cancellous bone. RESULTS:No patient experienced serious adverse events. Postoperative mean KOOS in "symptoms" subscale had not changed significantly from pre-operation until 24 months; whereas those in the other four subscales were significantly higher than pre-operation at 12 and 24 months. Second-look arthroscopy showed completely filled grafted sites, with regenerate cartilaginous surfaces flushed with surrounding native joint surface. Microscopically, regenerated cartilage appeared hyaline. CONCLUSION:This novel construct for chondrocyte implantation is safe for surgical application in knee. It repairs osteochondral lesions of femoral condyles by successful regeneration of hyaline cartilage.


Chiang H,Liao CJ,Hsieh CH,Shen CY,Huang YY,Jiang CC




Has Abstract


2013-04-01 00:00:00














  • A positive association of smoking and articular knee joint cartilage in healthy people.

    abstract:OBJECTIVE:To determine whether smoking affects knee cartilage in healthy adults by examining the association of tobacco use with tibial cartilage volume and tibiofemoral cartilage defects. METHODS:Two hundred and ninety-seven healthy adult subjects were recruited from an existing cohort examining healthy aging, the Me...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Racunica TL,Szramka M,Wluka AE,Wang Y,English DR,Giles GG,O'Sullivan R,Cicuttini FM

    更新日期:2007-05-01 00:00:00

  • Efficacy of a combination of FCHG49 glucosamine hydrochloride, TRH122 low molecular weight sodium chondroitin sulfate and manganese ascorbate in the management of knee osteoarthritis.

    abstract:OBJECTIVES:The objective of this study was to evaluate the oral combination of glucosamine HCl, sodium chondroitin sulfate and manganese ascorbate for the treatment of osteoarthritis (OA) of the knee. DESIGN:A randomized placebo-controlled study design was implemented. We recruited 93 patients with OA of the knee from...

    journal_title:Osteoarthritis and cartilage

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Das A Jr,Hammad TA

    更新日期:2000-09-01 00:00:00

  • Validation of cartilage volume and thickness measurements in the human shoulder with quantitative magnetic resonance imaging.

    abstract:OBJECTIVE:To validate quantitative magnetic resonance imaging (qMRI) for the assessment of cartilage volume and thickness in thin and curved cartilage layers, such as the shoulder. METHODS:Eight shoulder specimens from healthy individuals (aged 31-69 years) were investigated using a 3D gradient echo sequence with sele...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Graichen H,Jakob J,von Eisenhart-Rothe R,Englmeier KH,Reiser M,Eckstein F

    更新日期:2003-07-01 00:00:00

  • Long-term periarticular bone adaptation in a feline knee injury model for post-traumatic experimental osteoarthritis.

    abstract:OBJECTIVES:This study investigates the long-term changes of the periarticular bone, including cancellous bone and the subchondral plate, in an anterior cruciate ligament (ACL)-transected cat for post-traumatic osteoarthritis (OA). These periarticular bone changes are related to the health of all knee tissues including ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Boyd SK,Müller R,Leonard T,Herzog W

    更新日期:2005-03-01 00:00:00

  • Increased rate of hysterectomy in women undergoing surgery for osteoarthritis of the knee.

    abstract::We performed a case-controlled study to determine whether previous gynecological surgery is associated with severe osteoarthritis (OA) of the knee, the hip or the spine, as well as mild OA of the knee. Patients who underwent surgical treatment for OA were defined as having severe OA. Patients with knee pain and radiog...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Inoue K,Ushiyama T,Kim Y,Shichikawa K,Nishioka J,Hukuda S

    更新日期:1995-09-01 00:00:00

  • Tibial cancellous bone changes in patients with knee osteoarthritis. A short-term longitudinal study using Fractal Signature Analysis.

    abstract:OBJECTIVE:To quantify tibial cancellous bone changes over 24 months in patients with medial compartment knee osteoarthritis (OA) subdivided into those with slow or detectable joint space narrowing (JSN). METHOD:Digitised macroradiographs (4x) were obtained at baseline and 24 months from 40 patients (F:26) and subdivid...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Messent EA,Ward RJ,Tonkin CJ,Buckland-Wright C

    更新日期:2005-06-01 00:00:00

  • Cartilage loss in radiographically normal knees depends on radiographic status of the contralateral knee - data from the Osteoarthritis Initiative.

    abstract:OBJECTIVE:To test whether radiographically normal knees with contralateral radiographic knee osteoarthritis (OA), but without contralateral trauma history, display greater cartilage thickness loss than knees from subjects with bilaterally radiographically normal knees. METHODS:828 radiographically normal knees (Kellgr...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Eckstein F,Maschek S,Roemer FW,Duda GN,Sharma L,Wirth W

    更新日期:2019-02-01 00:00:00

  • T1ρ and T2 relaxation times are associated with progression of hip osteoarthritis.

    abstract:OBJECTIVE:To evaluate whether baseline T1ρ and T2 relaxation times of hip cartilage are associated with magnetic resonance imaging (MRI) based progression of hip osteoarthritis (OA) at 18 months. METHODS:3T MRI studies of the hip were obtained at baseline and 18-month follow-up for 54 subjects without evidence of seve...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Gallo MC,Wyatt C,Pedoia V,Kumar D,Lee S,Nardo L,Link TM,Souza RB,Majumdar S

    更新日期:2016-08-01 00:00:00

  • Long-term effect of removal of knee joint loading on cartilage quality evaluated by delayed gadolinium-enhanced magnetic resonance imaging of cartilage.

    abstract:OBJECTIVE:Ankle fracture patients were used as a model to study the long-term effect of the removal of joint loading on knee cartilage quality in human subjects. DESIGN:The knees of 10 patients with ipsilateral ankle fractures were investigated using delayed gadolinium-enhanced magnetic resonance imaging of cartilage ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Owman H,Tiderius CJ,Ericsson YB,Dahlberg LE

    更新日期:2014-07-01 00:00:00

  • Influence of oxygen tension on nitric oxide and prostaglandin E2 synthesis by bovine chondrocytes.

    abstract:OBJECTIVES:To determine the in vitro effects of oxygen tension on interleukin (IL)-1beta induced nitric oxide (*NO) and prostaglandin E(2) (PGE(2)) production by bovine chondrocytes. DESIGN:Enzymatically isolated bovine chondrocytes were cultured for different periods in suspension in 21 (atmospheric), 5 or 1% (low) o...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mathy-Hartert M,Burton S,Deby-Dupont G,Devel P,Reginster JY,Henrotin Y

    更新日期:2005-01-01 00:00:00

  • Reliability, reproducibility and variability of the traditional Histologic/Histochemical Grading System vs the new OARSI Osteoarthritis Cartilage Histopathology Assessment System.

    abstract:OBJECTIVE:For many years, the Histologic/Histochemical Grading System (HHGS) for osteoarthritis monitoring has been used as a histological scoring system for the quality of cartilage. There are, however, some limitations using this grading system. The goal of the investigation presented in this paper was to examine the...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Custers RJ,Creemers LB,Verbout AJ,van Rijen MH,Dhert WJ,Saris DB

    更新日期:2007-11-01 00:00:00

  • Osteoarthritis year in review 2019: imaging.

    abstract:OBJECTIVE:To provide a narrative review of original articles on osteoarthritis (OA) imaging published between April 1, 2018 and March 30, 2019. METHODS:All original research articles on OA imaging published in English between April 1, 2018 and March 30, 2019 were identified using a PubMed database search. The search t...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Kijowski R,Demehri S,Roemer F,Guermazi A

    更新日期:2020-03-01 00:00:00

  • Validation of the Dutch version of the Hip disability and Osteoarthritis Outcome Score.

    abstract:INTRODUCTION:The Hip disability and Osteoarthritis Outcome Score (HOOS) was constructed in Sweden; this questionnaire has proved to be valid for persons with hip disability with or without hip osteoarthritis (OA) and with high demands of physical function. OBJECTIVE:The objective of this study was to evaluate the inte...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: de Groot IB,Reijman M,Terwee CB,Bierma-Zeinstra SM,Favejee M,Roos EM,Verhaar JA

    更新日期:2007-01-01 00:00:00

  • Clinical, biochemical and imaging methods of assessing osteoarthritis and clinical trials with agents claiming 'chondromodulating' activity.

    abstract::This paper reviews, in a first part, methods used for the clinical assessment of osteoarthritis (OA) with special reference, in a second part, to trials of drugs claimed to be chondromodulating agents. The agents examined include glycosaminoglycan-peptide complex (GP-C, Rumalon) and glycosaminoglycan-polysulfate (GAGP...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Theiler R,Ghosh P,Brooks P

    更新日期:1994-03-01 00:00:00

  • Early cathepsin K degradation of type II collagen in vitro and in vivo in articular cartilage.

    abstract:OBJECTIVE:To characterize the initial events in the cleavage of type II collagen mediated by cathepsin K and demonstrate the presence of the resulting products in human and equine articular osteoarthritic cartilage. DESIGN:Equine type II collagen was digested with cathepsin K and the cleavage products characterized by...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mort JS,Beaudry F,Théroux K,Emmott AA,Richard H,Fisher WD,Lee ER,Poole AR,Laverty S

    更新日期:2016-08-01 00:00:00

  • Osteoarthritis year in review 2019: biomarkers (biochemical markers).

    abstract:OBJECTIVE:To provide an insightful summary of studies on biochemical markers for osteoarthritis (OA). DESIGN:Two investigators systematically searched the electronic PubMed database for clinical studies into soluble biochemical markers for OA in humans that were published between 01-03-2018 and 01-03-2019. Data from s...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: van Spil WE,Szilagyi IA

    更新日期:2020-03-01 00:00:00

  • Magnetic resonance imaging (MRI) of articular cartilage in knee osteoarthritis (OA): morphological assessment.

    abstract:OBJECTIVE:Magnetic resonance imaging (MRI) is a three-dimensional imaging technique with unparalleled ability to evaluate articular cartilage. This report reviews the current status of morphological assessment of cartilage with quantitative MRI (qMRI), and its relevance for identifying disease status, and monitoring pr...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Eckstein F,Cicuttini F,Raynauld JP,Waterton JC,Peterfy C

    更新日期:2006-01-01 00:00:00

  • Differential induction and regulation of matrix metalloproteinases in osteoarthritic tissue and fluid synovial fibroblasts.

    abstract:OBJECTIVES:To investigate the secretion profiles of matrix metalloproteinases (MMP) and their inhibitors (TIMP) in synovial fluid-derived fibroblasts and to compare them with those of tissue-derived fibroblasts. METHODS:Fibroblast cultures established from synovial tissues (TSC) and fluids (FSC) of the same OA patient...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fuchs S,Skwara A,Bloch M,Dankbar B

    更新日期:2004-05-01 00:00:00

  • Establishment of a bipotent cell line CL-1 which differentiates into chondrocytes and adipocytes from adult mouse.

    abstract:OBJECTIVE:Establishment of a clonal bipotent chondroprogenitor cell line from adult mouse to provide a new tool for the elucidation of chondrogenesis in adult animal. DESIGN:A clonal cell line CL-1 was established from tibia of adult mouse. Differentiation of CL-1 was characterized in monolayer culture. Effects of gro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kitamura H

    更新日期:2004-01-01 00:00:00

  • Modulation of inflammation by chondroitin sulfate.

    abstract:OBJECTIVE AND METHODS:To evaluate the immune-modulator effect of chondroitin sulfate (CS) by means of the review of the literature. RESULTS:Inflammatory reactions are primarily originated by infectious agents, immune reactions and by sterile tissue lesions that activate membrane receptors by means of pathogen-associat...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Vallières M,du Souich P

    更新日期:2010-06-01 00:00:00

  • Effectiveness of autologous chondrocyte implantation in cartilage repair of the knee: a systematic review of controlled trials.

    abstract:OBJECTIVE:The relative differences in effectiveness of subchondral stimulation, osteochondral grafts, and autologous chondrocyte implantation (ACI) are still unclear. It is the objective of this study to systematically review the literature on ACI compared to other treatments by clinical outcome and the quality of the ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Vavken P,Samartzis D

    更新日期:2010-06-01 00:00:00

  • Notch signaling in postnatal joint chondrocytes, but not subchondral osteoblasts, is required for articular cartilage and joint maintenance.

    abstract:OBJECTIVE:Notch signaling has been identified as a critical regulator in cartilage development and joint maintenance, and loss of Notch signaling in all joint tissues results in an early and progressive osteoarthritis (OA)-like pathology. This study investigated the targeted cell population within the knee joint in whi...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Liu Z,Ren Y,Mirando AJ,Wang C,Zuscik MJ,O'Keefe RJ,Hilton MJ

    更新日期:2016-04-01 00:00:00

  • Comparison of mechanical debridement and radiofrequency energy for chondroplasty in an in vivo equine model of partial thickness cartilage injury.

    abstract:OBJECTIVE:The purpose of this study was to develop a long-term model of cartilage injury that could be used to compare the effects of radiofrequency energy (RFE) and mechanical debridement as a treatment. METHODS:Partial thickness fibrillation of patellar cartilage was created in 16 mature ponies. Three months after t...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Edwards RB 3rd,Lu Y,Uthamanthil RK,Bogdanske JJ,Muir P,Athanasiou KA,Markel MD

    更新日期:2007-02-01 00:00:00

  • Osteoarthritis year 2012 in review: imaging.

    abstract:PURPOSE:This article reviews original publications related to imaging in osteoarthritis (OA) published in English from September 2011 through March 2012. In vitro data and animal studies are not covered. METHODS:To extract relevant studies, an extensive PubMed database search was performed using the query terms "osteo...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Roemer FW,Guermazi A

    更新日期:2012-12-01 00:00:00

  • Three-dimensional hip cartilage quality assessment of morphology and dGEMRIC by planar maps and automated segmentation.

    abstract:OBJECTIVE:The quantitative interpretation of hip cartilage magnetic resonance imaging (MRI) has been limited by the difficulty of identifying and delineating the cartilage in a three-dimensional (3D) dataset, thereby reducing its routine usage. In this paper a solution is suggested by unfolding the cartilage to planar ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Siversson C,Akhondi-Asl A,Bixby S,Kim YJ,Warfield SK

    更新日期:2014-10-01 00:00:00

  • Effects of SKI 306X, a new herbal agent, on proteoglycan degradation in cartilage explant culture and collagenase-induced rabbit osteoarthritis model.

    abstract:OBJECTIVE:Protective effects of SKI 306X, a natural herbal product extracted from three herbs Clematis mandshurica, Trichosanthes kirilowii, and Prunella vulgaris, on articular cartilage was examined and compared with other osteoarthritis (OA) drugs using in vitro and in vivo models. METHODS:In vitro culture of rabbit...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Choi JH,Choi JH,Kim DY,Yoon JH,Youn HY,Yi JB,Rhee HI,Ryu KH,Jung K,Han CK,Kwak WJ,Cho YB

    更新日期:2002-06-01 00:00:00

  • Osteoarthritis year 2012 in review: biomarkers.

    abstract:PURPOSE:Biomarkers provide useful diagnostic information by detecting cartilage degradation in osteoarthritis (OA), reflecting disease-relevant biological activity and predicting the course of disease progression. They also serve as surrogate endpoints in the drug discovery process. The aim of this narrative review was...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Mobasheri A

    更新日期:2012-12-01 00:00:00

  • Nondestructive assessment of sGAG content and distribution in normal and degraded rat articular cartilage via EPIC-microCT.

    abstract:OBJECTIVE:The objective of this study was to evaluate the feasibility of quantifying the Equilibrium Partitioning of an Ionic Contrast agent via Microcomputed Tomography (EPIC-microCT) to nondestructively assess sulfated glycosaminoglycan (sGAG) content and distribution in rat articular cartilage ex vivo, and in doing ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Xie L,Lin AS,Guldberg RE,Levenston ME

    更新日期:2010-01-01 00:00:00

  • Modeling and predicting osteoarthritis progression: data from the osteoarthritis initiative.

    abstract:OBJECTIVE:The goal of this study was to model the longitudinal progression of knee osteoarthritis (OA) and build a prognostic tool that uses data collected in 1 year to predict disease progression over 8 years. DESIGN:To model OA progression, we used a mixed-effects mixture model and 8-year data from the Osteoarthriti...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Halilaj E,Le Y,Hicks JL,Hastie TJ,Delp SL

    更新日期:2018-12-01 00:00:00

  • Subchondral and epiphyseal bone remodeling following surgical transection and noninvasive rupture of the anterior cruciate ligament as models of post-traumatic osteoarthritis.

    abstract:OBJECTIVE:Animal models are frequently used to study post-traumatic osteoarthritis (PTOA). A common anterior cruciate ligament (ACL) injury model is surgical transection, which may introduce confounding factors from surgery. Noninvasive models could model human injury more closely. The purpose of this study was to comp...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Maerz T,Kurdziel M,Newton MD,Altman P,Anderson K,Matthew HW,Baker KC

    更新日期:2016-04-01 00:00:00