Abstract:
OBJECTIVE:To define for the first time the transcriptomes of normal and end-stage osteoarthritis (OA) hip cartilage. MATERIALS AND METHODS:RNA was isolated from cartilage within 2h of joint replacement surgery. Gene expression was analyzed using Agilent GeneSpring GX 11 following hybridization to Illumina Human HT-12 V3 microarrays. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was used to validate the expression of six genes identified by microarray as differentially expressed. Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) were used to investigate enriched functions or canonical pathways amongst differentially expressed genes respectively. RESULTS:In total we identified 998 differentially expressed genes (fold change ≥ ±1.5, P-value ≤ 0.01) between neck of femur fracture (NOF) (n = 10) and OA hip (n = 9) patient cartilage. These differentially expressed genes were enriched within 71 canonical pathways. A comparison between a comparable knee dataset(20) only identified 229 genes similarly differentially expressed although remarkably 34 canonical pathways overlapped between experiments. CONCLUSIONS:This study is the first to report a comprehensive gene expression analysis of human hip OA cartilage compared to control (NOF) cartilage at the whole-genome level. Our differential gene expression dataset shows excellent correlation with similar defined studies using comparable tissue but reveals discord between hip and knee OA at the individual gene status but with commonality with regards the molecular pathways involved.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Xu Y,Barter MJ,Swan DC,Rankin KS,Rowan AD,Santibanez-Koref M,Loughlin J,Young DAdoi
10.1016/j.joca.2012.05.006subject
Has Abstractpub_date
2012-09-01 00:00:00pages
1029-38issue
9eissn
1063-4584issn
1522-9653pii
S1063-4584(12)00833-3journal_volume
20pub_type
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更新日期:2007-02-01 00:00:00
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更新日期:2019-12-01 00:00:00
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更新日期:2017-12-01 00:00:00
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更新日期:2011-07-01 00:00:00
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更新日期:2002-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2016.08.009
更新日期:2017-01-01 00:00:00
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doi:10.1016/j.joca.2005.07.003
更新日期:2005-12-01 00:00:00
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更新日期:2018-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2016.03.005
更新日期:2016-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1053/joca.1998.0224
更新日期:1999-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1053/joca.1999.0300
更新日期:2000-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2012.08.004
更新日期:2012-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2006.03.015
更新日期:2006-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2017.08.016
更新日期:2017-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2007.04.001
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更新日期:2013-12-01 00:00:00
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pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2006-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2020.07.004
更新日期:2020-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-12-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2014.05.024
更新日期:2014-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2016.06.012
更新日期:2016-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2005.03.002
更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.joca.2016.01.340
更新日期:2016-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-08-01 00:00:00