Abstract：
OBJECTIVE:Disturbances of the proteoglycan metabolism play an essential role in the pathology of osteoarthritis. The extracellular matrix proteoglycan, perlecan, has lately been identified as a cell biological factor in cartilage development and maintenance. We investigated the tissue distribution of perlecan, the relation between the level of the protein and its mRNA and which type of cell, type 1 chondrocytes or elongated secretory type 2 cells, produces perlecan in late stages of osteoarthritis. METHODS:In 10 patients suffering from late-stage osteoarthritis tissue samples taken from a macroscopically intact area and the area adjacent to the main cartilage defect were investigated. We performed quantitative immunogold histochemistry and in situ hybridization in vivo and determined the level of perlecan mRNA with the help of real-time RT-PCR in native cartilage tissue and in cultured cells. RESULTS:In vivo, an increased level of perlecan protein was found in the area adjacent to the main defect. A 45% rise in the level of perlecan mRNA secreted by elongated secretory type 2 cells in comparison to type 1 chondrocytes was detected. Type 2 cells also translated the highest levels of perlecan to be deposited mainly in the pericellular matrix, and also in the interterritorial matrix in late stages of osteoarthritis. Also in vitro, type 2 cells showed a 50% higher level of mRNA for perlecan. CONCLUSION:We found evidence that perlecan is involved in the pathogenesis of late stages of osteoarthritis. The levels of perlecan protein and mRNA are up-regulated especially by the elongated secretory type 2 cells in the area adjacent to the main cartilage defect. This might be seen as an attempt on the part of the cartilage tissue to stabilize the extracellular matrix.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Tesche F,Miosge Ndoi
10.1016/j.joca.2004.07.004keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
852-62issue
11eissn
1063-4584issn
1522-9653pii
S1063-4584(04)00138-4journal_volume
12pub_type
杂志文章abstract：OBJECTIVE:Cam hip shape morphology is a recognised cause of femoroacetabular impingement (FAI) and is associated with hip osteoarthritis. Our aim was to systematically review the available epidemiological evidence assessing the prevalence of cam hip shape morphology in the general population and any studied subgroups i...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2015.12.020
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVE:To investigate the in vivo role of the IRE1/XBP1 unfolded protein response (UPR) signaling pathway in cartilage. DESIGN:Xbp1(flox/flox).Col2a1-Cre mice (Xbp1(CartΔEx2)), in which XBP1 activity is ablated specifically from cartilage, were analyzed histomorphometrically by Alizarin red/Alcian blue skeletal pre...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.01.001
更新日期：2015-04-01 00:00:00
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更新日期：2019-12-01 00:00:00
abstract：OBJECTIVE:The purposes of this study were to assess whether local anesthetics (LAs), such as ropivacaine and bupivacaine, could induce apoptosis of rabbit annulus fibrosus (AF) cells in vitro and further to explore the possible underlying mechanism. METHODS:Rabbit AF cells at second passage were treated with saline so...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.05.013
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:To determine how well measures of hip geometry can predict radiological incident hip osteoarthritis (HOA) compared to well known clinical risk factors. DESIGN:The study population is part of the Rotterdam Study, a prospective population-based cohort. Baseline pelvic radiographs were used to measure hip geome...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2013.06.012
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVES:Osteoarthritis (OA) is the most prevalent chronic disease in the elderly, and it is generally diagnosed at an advanced state when treatment is difficult if not impossible. The early form of OA is characterized by an elevated water content in the cartilage tissue. The purpose of this study was to verify in vi...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2002.0847
更新日期：2002-12-01 00:00:00
abstract：OBJECTIVES:The etiology of degenerative meniscus tear is unclear but could be related to a generalized osteoarthritic disease process. We studied whether radiographic hand osteoarthritis (OA) is associated with meniscus damage. METHODS:We examined 974 persons aged 50-90 years drawn via census tract data and random-dig...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.08.005
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVE:Osteoarthritis (OA) is a common complex disease with strong heritable components. In this study, we investigated the association between four putatively functional genetic variants in KLOTHO gene, a strong ageing-related gene, and hand OA in a large female Caucasian population. METHODS:Subjects (n=1015, age ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2006.12.002
更新日期：2007-06-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.03.013
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVE:Femoroacetabular impingement (FAI) is a pathomechanical process, which may cause hip pain, disability and early development of hip osteoarthritis (OA) in young and active adults. Patients with FAI experience functional disability during dynamic weight-bearing activities, which could originate from weakness of...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2011.04.001
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:Marked differences exist between human knee and ankle joints regarding risks and progression of osteoarthritis (OA). Pathomechanisms of degenerative joint disease may therefore differ in these joints, due to differences in tissue structure and function. Focusing on structural issues, which are design goals fo...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2018.08.003
更新日期：2018-12-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.11.021
更新日期：2015-03-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.08.017
更新日期：2016-02-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2001.0424
更新日期：2001-08-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2000.0368
更新日期：2001-02-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2009.10.009
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:To investigate changes in knee 3D bone shape over the first 5 years after acute anterior cruciate ligament (ACL) injury in participants of the randomized controlled KANON-trial. METHODS:Serial MR images over 5 years from 121 young (32 women, mean age 26.1 years) adults with an acute ACL tear in a previously...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joca.2018.12.021
更新日期：2019-04-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,meta分析,评审
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更新日期：2017-06-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.07.004
更新日期：2016-12-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2012.10.007
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:Ankle fracture patients were used as a model to study the long-term effect of the removal of joint loading on knee cartilage quality in human subjects. DESIGN:The knees of 10 patients with ipsilateral ankle fractures were investigated using delayed gadolinium-enhanced magnetic resonance imaging of cartilage ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.04.021
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:The relative differences in effectiveness of subchondral stimulation, osteochondral grafts, and autologous chondrocyte implantation (ACI) are still unclear. It is the objective of this study to systematically review the literature on ACI compared to other treatments by clinical outcome and the quality of the ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2010.03.005
更新日期：2010-06-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(03)00149-3
更新日期：2003-09-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2006.12.005
更新日期：2007-05-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joca.2014.03.006
更新日期：2014-05-01 00:00:00
abstract：INTRODUCTION:In clinical tissue-engineering-based approaches to articular cartilage repair, various types of flap are frequently used to retain an implanted construct within the defect, and they are usually affixed by suturing. We hypothesize that the suturing of articular cartilage is associated with a loss of chondro...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2008.01.009
更新日期：2008-09-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.03.001
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE:Calcitonin (CT) has been recently shown to exhibit direct protective effects on articular cartilage against joint degenerative disease. It has been proposed that CT might act via the CT receptor (CTR) to activate the cyclic AMP (cAMP) pathway and protect type II collagen degradation. In this study, we investi...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.08.003
更新日期：2008-04-01 00:00:00
abstract：PURPOSE:Our aim was to introduce a simplified MRI instrument, Rapid OsteoArthritis MRI Eligibility Score (ROAMES), for defining structural eligibility of patients for inclusion in disease-modifying osteoarthritis drug trials using a tri-compartmental anatomic approach that enables stratification of knees into different...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2019.08.005
更新日期：2020-01-01 00:00:00
