Generation of functional eggs and sperm from cryopreserved whole testes.

abstract：:The adult brain exhibits a local increase in cortical blood flow in response to external stimulus. However, broadly varying hemodynamic responses in the brains of newborn and young infants have been reported. Particular controversy exists over whether the "true" neonatal response to stimulation consists of a decrease ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kozberg MG,Chen BR,DeLeo SE,Bouchard MB,Hillman EM

更新日期：2013-03-12 00:00:00

abstract：:One goal of regenerative medicine is to repair damaged tissue. This requires not only generating new cells of the proper phenotype, but also selecting for those that properly integrate into sites of injury. In our laboratory we are using a cell-migration-based in vivo selection system to generate antibodies that induc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han KH,Arlian BM,Macauley MS,Paulson JC,Lerner RA

更新日期：2018-01-16 00:00:00

abstract：:Mesencephalic dopamine neurons from the embryonic mouse brain were dissociated, aggregated in vitro in the presence of dissociated cells from appropriate or inappropriate target neuron areas, and visualized by the Falck-Hillarp histofluorescence technique after exposure to 1 microM exogenous dopamine. When aggregated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hemmendinger LM,Garber BB,Hoffmann PC,Heller A

更新日期：1981-02-01 00:00:00

abstract：:Morphological and genetic characters from cyprinid fishes of the genus Gila were examined to assess a hypothesized hybrid origin of Gila seminuda from the Virgin River, Arizona-Nevada-Utah. The presumed parents, Gila robusta robusta and Gila elegans, are clearly differentiated from one another based on morphology, all...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeMarais BD,Dowling TE,Douglas ME,Minckley WL,Marsh PC

更新日期：1992-04-01 00:00:00

abstract：:We use alternating-laser excitation to achieve fluorescence-aided molecule sorting (FAMS) and enable simultaneous analysis of biomolecular structure and interactions at the level of single molecules. This was performed by labeling biomolecules with fluorophores that serve as donor-acceptor pairs for Förster resonance ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kapanidis AN,Lee NK,Laurence TA,Doose S,Margeat E,Weiss S

更新日期：2004-06-15 00:00:00

abstract：:Deuterium oxide, D(2)O, increases the temperature-tolerance of Drosophila pseudoobscura when it is administered to adult flies as a sucrose solution. The effect is very rapidly exerted; it is detected within 10 min after the flies have a brief (10 min) opportunity to drink. This increased resistance to heat-death sure...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pittendrigh CS,Cosbey ES

更新日期：1974-02-01 00:00:00

abstract：:Staphylococcus aureus is a leading cause of both nosocomial and community-acquired infection. Biofilm formation at the site of infection reduces antimicrobial susceptibility and can lead to chronic infection. During biofilm formation, a subset of cells liberate cytoplasmic proteins and DNA, which are repurposed to for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeFrancesco AS,Masloboeva N,Syed AK,DeLoughery A,Bradshaw N,Li GW,Gilmore MS,Walker S,Losick R

更新日期：2017-07-18 00:00:00

abstract：:The values of the moment of inertia of the ground-state band of (168)Hf from J = 0(+) to 22(+) are interpreted as involving a revolving cluster with composition increasing steadily from p(10.5)n(14) to p(20.5)n(24), with radius of revolution 6.390 fm. The excited bands from 14(+) to 34(+), 21(-) to 33(-), and 18(-) to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pauling L

更新日期：1984-05-01 00:00:00

abstract：:Oxygen isotherms of human hemoglobin measured in distilled water and in solutions of sodium chloride in the concentration range from 0.02 to 3.0 M indicate that the oxygen affinity decreases up to about 1 M salt and then begins to increase. The isotherms obtained in the range from 0.02 to 0.6 M sodium chloride, at 37 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haire RN,Hedlund BE

更新日期：1977-10-01 00:00:00

abstract：:Myoglobin-mediated oxygen delivery to intracellular mitochondria is demonstrated in cardiac myocytes isolated from the hearts of mature rats. Myocytes are held at high ambient oxygen pressure, 40-340 torr (5-45 kPa); sarcoplasmic myoglobin is fully oxygenated. In this condition oxygen availability does not limit respi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wittenberg BA,Wittenberg JB

更新日期：1987-11-01 00:00:00

abstract：:Relative cerebral glucose metabolism was examined with positron-emission tomography (PET) as a measure of neuronal activation during performance of the classically conditioned eyeblink response in 12 young adult subjects. Each subject received three sessions: (i) a control session with PET scan in which unpaired prese...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Logan CG,Grafton ST

更新日期：1995-08-01 00:00:00

abstract：:The functional consequences of interrupting in utero binocular interactions were studied by recording from single cells in area 17 of adult cats that had one eye removed at least 2 wk before birth. In these animals all cortical neurons could be driven by the remaining eye, and in tangential microelectrode penetrations...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shook BL,Maffei L,Chalupa LM

更新日期：1985-06-01 00:00:00

abstract：:Inorganic elements, although required only in trace amounts, permit life and primary productivity because of their functions in catalysis. Every organism has a minimal requirement of each metal based on the intracellular abundance of proteins that use inorganic cofactors, but elemental sparing mechanisms can reduce th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kropat J,Gallaher SD,Urzica EI,Nakamoto SS,Strenkert D,Tottey S,Mason AZ,Merchant SS

更新日期：2015-03-03 00:00:00

abstract：:Corticotropin (ACTH) and melanotropin (MSH) peptides (melanocortins) are produced not only in the pituitary but also in the brain, with highest concentrations in the arcuate nucleus of the hypothalamus and the commisural nucleus of the solitary tract. We have identified a receptor for MSH and ACTH peptides that is spe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roselli-Rehfuss L,Mountjoy KG,Robbins LS,Mortrud MT,Low MJ,Tatro JB,Entwistle ML,Simerly RB,Cone RD

更新日期：1993-10-01 00:00:00

abstract：:The chemical dynamics of lakes and streams affect their suitability as aquatic habitats and as water supplies for human needs. Because water quality is typically monitored only weekly or monthly, however, the higher-frequency dynamics of stream chemistry have remained largely invisible. To illuminate a wider spectrum ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirchner JW,Neal C

更新日期：2013-07-23 00:00:00

abstract：:Islet transplantation offers a potential therapy to restore glucose homeostasis in type 1 diabetes patients. However, islet transplantation is not routinely successful because most islet recipients gradually lose graft function. Furthermore, serological markers of islet function are insensitive to islet loss until the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Y,Dang H,Middleton B,Zhang Z,Washburn L,Stout DB,Campbell-Thompson M,Atkinson MA,Phelps M,Gambhir SS,Tian J,Kaufman DL

更新日期：2006-07-25 00:00:00

abstract：:Systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes parkinsonism in humans and subhuman primates, but not in rats and many other laboratory animals; mice are intermediate in their susceptibility. Since MPTP causes selective dopaminergic neurotoxicity when infused directly into rat sub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalaria RN,Mitchell MJ,Harik SI

更新日期：1987-05-01 00:00:00

abstract：:Three protein kinases (ATP:protein phosphotransferase, EC 2.7.1.37) were detected when the soluble fraction of rabbit kidney medulla was chromatographed on DEAE-cellulose with a linear NaC1 gradient. The first two kinases eluted (Peak 1 and Peak II) were cyclic-AMP-dependent, wheras Peak III was cyclic-AMP-independent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlender KK,Reimann EM

更新日期：1975-06-01 00:00:00

abstract：:Gold nanoparticles quench the fluorescence of cationic polyfluorene with Stern-Volmer constants (KSV) approaching 1011 M-1, several orders of magnitude larger than any previously reported conjugated polymer-quencher pair and 9-10 orders of magnitude larger than small molecule dye-quencher pairs. The dependence of KSV ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan C,Wang S,Hong JW,Bazan GC,Plaxco KW,Heeger AJ

更新日期：2003-05-27 00:00:00

abstract：:The attachment of chondrocytes to collagen substrates is stimulated by serum but not by fibronectin. The active material in serum was partially purified and was shown to be a protein by its sensitivity to trypsin and heat and its chromatographic properties. This factor, which we have named chondronectin, is distinct f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hewitt AT,Kleinman HK,Pennypacker JP,Martin GR

更新日期：1980-01-01 00:00:00

abstract：:The assembly of clathrin-coated vesicles on Golgi membranes is initiated by the GTP-binding protein ADP ribosylation factor (ARF), which generates high-affinity membrane-binding sites for the heterotetrameric AP-1 adaptor complex. Once bound, the AP-1 recruits clathrin triskelia, which polymerize to form the coat. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu Y,Drake MT,Kornfeld S

更新日期：1999-04-27 00:00:00

abstract：:Retinopathy of prematurity is a blinding disease, initiated by lack of retinal vascular growth after premature birth. We show that lack of insulin-like growth factor I (IGF-I) in knockout mice prevents normal retinal vascular growth, despite the presence of vascular endothelial growth factor, important to vessel devel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hellstrom A,Perruzzi C,Ju M,Engstrom E,Hard AL,Liu JL,Albertsson-Wikland K,Carlsson B,Niklasson A,Sjodell L,LeRoith D,Senger DR,Smith LE

更新日期：2001-05-08 00:00:00

abstract：:Examples from various plant and animal groups indicate that there has been a general increase in potential versatility of form, determined by the number and range of independently varying morphogenetic parameters, among taxa appearing at successively younger stages in the fossil record. Taxa or body plans with higher ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vermeij GJ

更新日期：1973-07-01 00:00:00

abstract：:The classical Euler-Poisson equations describing the motion of a heavy rigid body about a fixed point are generalized to arbitrary Lie algebras as Hamiltonian systems on coad-joint orbits of a tangent bundle Lie group. the N-dimensional Lagrange and symmetric heavy top are thereby shown to be completely integrable. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratiu T

更新日期：1981-03-01 00:00:00

abstract：:Impaired magnesium reabsorption in patients with TRPM6 gene mutations stresses an important role of TRPM6 (melastatin-related TRP cation channel) in epithelial magnesium transport. While attempting to isolate full-length TRPM6, we found that the human TRPM6 gene encodes multiple mRNA isoforms. Full-length TRPM6 varian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chubanov V,Waldegger S,Mederos y Schnitzler M,Vitzthum H,Sassen MC,Seyberth HW,Konrad M,Gudermann T

更新日期：2004-03-02 00:00:00

abstract：:Nociceptive information is modulated by a large number of endogenous signaling agents that change over the course of recovery from injury. This plasticity makes understanding regulatory mechanisms involved in descending inhibition of pain scientifically and clinically important. Neurons that synthesize the neuropeptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dimitrov EL,Kuo J,Kohno K,Usdin TB

更新日期：2013-08-06 00:00:00

abstract：:We have used the spreading behavior of small drops of several fluorocarbon fluids and silicone oil on air-liquid interfaces to measure the surface tension of lungs in situ. The test fluids were calibrated in a surface balance at 37 degrees on monolayers of dipalmitoylphosphatidylcholine. At particular surface tensions...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schürch S,Goerke J,Clements JA

更新日期：1976-12-01 00:00:00

abstract：:A minimalist Cys2His2 zinc finger peptide, Lys-Tyr-Ala-Cys-Ala-Ala-Cys-Ala-Ala-Ala-Phe-Ala-Ala-Lys-Ala-Ala-Leu-Ala- Ala-His-Ala-Ala-Ala-His-Ala-Lys, has been synthesized. Metal binding studies using Co2+ as a probe indicated that this peptide forms a 1:1 peptide/metal complex with a dissociation constant comparable to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michael SF,Kilfoil VJ,Schmidt MH,Amann BT,Berg JM

更新日期：1992-06-01 00:00:00

abstract：:Regulatory T cells (Treg) comprise multiple subsets and are important in controlling immunity and inflammation. However, the induction and mode of action of the various distinct Treg subsets remain ill defined, particularly in humans. Here, we describe a human CD8+ lymphocyte activation gene-3 (LAG-3)+CD25+FoxP3+ Treg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joosten SA,van Meijgaarden KE,Savage ND,de Boer T,Triebel F,van der Wal A,de Heer E,Klein MR,Geluk A,Ottenhoff TH

更新日期：2007-05-08 00:00:00

abstract：:The pattern of antibody specificities in sera from patients with myasthenia gravis (MG) was determined by the ability of monoclonal antibodies against defined determinants on the acetylcholine receptor molecule to inhibit binding of the serum antibodies to receptor from human muscle. We found that MG patients produce ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tzartos SJ,Seybold ME,Lindstrom JM

更新日期：1982-01-01 00:00:00