Abstract：
OBJECTIVES:Rheumatoid arthritis (RA) is associated with accelerated atherosclerosis and premature cardiovascular death. Anti-TNF therapy is thought to reduce clinical cardiovascular disease risk and improve vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to explore the effects of certolizumab pegol (CZP) on TNF-activated human aortic endothelial cells (HAoECs). METHODS:HAoECs were cultured in vitro and exposed to i) TNF alone, ii) TNF plus CZP, or iii) neither agent. Microarray analysis and quantitative polymerase chain reaction were used to analyse gene expression. Activation of NF-κB was investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was performed to detect microparticle release from HAoECs. RESULTS:TNF alone had strong effects on endothelial gene expression, while TNF and CZP together produced a global gene expression pattern similar to untreated controls. In particular, genes for E-selectin, VCAM-1 and ICAM-1 were significantly up-regulated by TNF treatment. Notably, the TNF/CZP cocktail prevented the up-regulation of these genes. TNF-induced nuclear translocation of NF-κB was abolished by treatment with CZP. In addition the increased production of endothelial microparticles in TNF-activated HAoECs was prevented by treatment with CZP. CONCLUSIONS:We have found at cellular level, that a clinically available TNF inhibitor, CZP i) reduces adhesion molecule expression; ii) prevents TNF-induced activation of the NF-κB pathway and iii) prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions.
journal_name
Clin Exp Rheumatoljournal_title
Clinical and experimental rheumatologyauthors
Heathfield SK,Parker B,Zeef LA,Bruce IN,Alexander MYsubject
Has Abstractpub_date
2013-03-01 00:00:00pages
225-33issue
2eissn
0392-856Xissn
1593-098Xpii
5818journal_volume
31pub_type
杂志文章abstract：:Autoimmune thyroid disease is common in patients with either systemic lupus erythematosus (SLE) or Sjögren's syndrome (SS); however, whether the risk is greater than in well matched controls is not clear. Antinuclear antibodies are found in the sera of patients with autoimmune thyroid disease, but there is no evidence...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：1996-05-01 00:00:00
abstract：OBJECTIVES:To evaluate the micro-RNA (miRNA) expression profile in patients with early psoriatic arthritis (PsA) in order to assess the role of miRNAs as potential PsA biomarkers. METHODS:The expression of 723 mature miRNAs in peripheral blood mononuclear cells of early PsA patients in comparison with early-rheumatoid...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVES:To determine the protein expression of TNFAIP3 in synovium and to show the capability of 6q23 intergenic SNPs, associated with rheumatoid arthritis (RA) susceptibility, to influence TNFAIP3 gene transcription. METHODS:Immunohistochemistry for TNFAIP3, NF-kB p65 and phosphorylated NF-kB p65 protein expressio...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:Monoclonal antibody against c-myc protein was used in the avidin-biotin-peroxidase complex procedure to examine frozen sections of psoriatic and normal skin and psoriatic synovium for the presence of c-myc protein. A significantly greater percentage (88%) of epithelial cells from psoriatic skin lesions stained for c-m...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:To test whether alternative complement pathway activation explains normal C1s-C1 inhibitor complex in hypocomplementemic (low CH50cl) patients with systemic lupus erythematosus, we examined alternative pathway hemolytic complement (CH50alt) factor B, and Ba fragment in hypocomplementemic sera with normal and with elev...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVES:To assess increased sacroiliac joint (SIJ) uptake on 18F-NaF PET/CT according to a qualitative and quantitative approach and to compare with MRI SIJ assessments for structural and inflammatory sacroiliitis in a population of 23 patients with spondyloarthritis (SpA) (IDRCB: 2012-A00568-35; ClinicalTrials.gov:...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2019-01-01 00:00:00
abstract：:A meta-analysis showed that methodological differences in prevalence studies such as a sample survey design or census design may be responsible for some of the variance in BS prevalence reported across countries, in addition to a true geographic variation. Efforts towards developing a data driven core set of outcome m...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVE:We investigated the presence of anti-GOR antibodies in patients with essential mixed cryoglobulinemia, since both autoimmune pathogenetic processes and a high prevalence of HCV infection are present in this syndrome. METHODS:We compared these cases to patients with HCV-related chronic active hepatitis or alc...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:We report herein the results of the cross-cultural adaptation and validation into the Latvian language of the parent's version of two health related quality of life instruments. The Childhood Health Assessment Questionnaire (CHAQ) is a disease specific health instrument that measures functional ability in daily living...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:Relationships between clinical features of psoriatic arthritis and HLA antigens were examined in 60 patients. HLA-B locus antigens, Bw38, B17, B27 and possibly Bw39 were significantly increased, while HLA-D and DR specificities were not. Cw6 was not studied. The relative risk for disease was doubled in patients having...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:Sjögren's syndrome is a multifactorial disease in which genetic and possibly viral factors play major roles. The recent development of an SS-like disease in patients infected with HIV-1 suggests a possible retrovirus. ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：1990-07-01 00:00:00
abstract：OBJECTIVES:Systemic lupus erythematosus (SLE) is a chronic autoimmune multi-organ disease with an unpredictable course. SLE causes functional disability, changes in body appearance, and psychological distress. When faced with SLE, patients have to implement coping strategies. Therefore, the aim of this study was to des...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2020-07-01 00:00:00
abstract：OBJECTIVES:Non-infectious aortitis often presents with non-specific symptoms leading to inappropriate diagnostic delay. We intend to describe the clinical spectrum and outcome of patients with aortitis diagnosed at a single centre. METHODS:We reviewed the clinical charts of patients diagnosed with non-infectious aorti...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVES:To evaluate the clinical characteristics and imaging results (CDS, 18-FDG-PET) of patients with large vessel giant cell arteritis (LV-GCA) presenting as fever of unknown origin (FUO). METHODS:From a series of 82 patients with GCA we identified 8 patients with FUO as initial disease manifestation. Clinical c...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：:Blockade of nerve growth factor (NGF) with antibodies is a promising strategy for treatment of chronic pain associated with osteoarthritis (OA). This narrative review describes the current status of NGF-blockade for the treatment of OA pain. We summarise briefly current evidence for the efficacy and risks of anti-NGF ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:Imaging plays a role in myositis assessment by detecting muscle changes indicative of pathology. This study was conducted to determine the ultrasonographic pattern of muscle involvement in patients with inclusion body myositis (IBM) through an assessment of muscle echointensity. METHODS:Sixty-two individual...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2018-11-01 00:00:00
abstract：:The classification criteria recently developed by the Assessment of Spondyloarthritis International Society (ASAS) highlighted a specific entity: non radiographic axial spondyloarthritis (nr-axSpA). Although more and more widely used in the literature as well as clinical trials, limits and profile of this entity is st...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVES:The Schnitzler syndrome is a rare inflammatory disorder, with a chronic urticaria-like rash and an IgM (rarely IgG) monoclonal gammopathy as cardinal features. Interleukin-1 β is regarded as the key mediator and the interleukin-1 receptor antagonist anakinra has been proposed as first-line treatment. This ca...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：:Several cases of patients with concomitant SLE and HIV infection have been reported in the literature; however, the effect of immunosuppressive therapy on HIV replication has not been described. We present the case of a 46 y/o woman with a ten-year history of HIV infection who was treated with IV cyclophosphamide for ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:Remission in psoriatic arthritis (PsA), albeit variably defined, is a desirable and achievable state, especially in the era of biologic therapy. Historically, studies have used remission criteria derived from rheumatoid arthritis (RA), which indicate that remission is seen in a greater percentage of patients than in R...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：:Twenty-three per cent of rheumatoid arthritis (RA) patients show an increase of serum IgA concentrations. To determine the role of mucous-associated lymphoid tissue (MALT) in the elevation of serum IgA in RA, we studied the serum secretory-IgA (s-IgA) in 63 RA patients and in 30 healthy controls. We also analysed the ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Patients with alkaptonuria lack homogentisate 1,2-dioxygenase leading to retention of homogentistic acid (HGA) in body fluids and eventually to tissue deposition of oxidation products, giving rise to the clinical picture of ochronosis. Ascorbic acid is a known inhibitor of the enzyme which catalyses the oxidation of h...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：: ...
journal_title：Clinical and experimental rheumatology
pub_type： 评论,信件
doi：
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVE:Behçet's disease (BD) is a unique systemic vasculitis involving both arteries and veins of all sizes. Since Fcgamma receptors (FcgammaR) are important in mediating various immune effector functions, FcgammaR gene polymorphisms may affect the susceptibility to systemic inflammatory diseases such as BD. The aim...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：OBJECTIVE:To determine the diagnostic and therapeutic approach used in clinical practice for the management of systemic lupus erythematosus (SLE) patients with primary SLE-mediated neuropsychiatric (NP) manifestations. METHODS:A questionnaire was drawn up to assess how clinicians manage various clinical manifestations...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,多中心研究
doi：
更新日期：1996-11-01 00:00:00
abstract：OBJECTIVES:Since the 1980 ACR classification criteria for systemic sclerosis (SSc) do not identify 20% with SSc, revised criteria are necessary. METHODS:Suggested new criteria from the literature were sent in random order to 96 SSc experts. A 3-round Delphi Consensus eliminated criteria. Then cluster analysis reduced ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVES:We aimed to 1) identify clinical and genetic associations of sacroiliitis (SI) in patients with psoriatic arthritis (PsA), and 2) describe the different radiographic patterns of SI in PsA and their clinical and genetic associations. METHODS:283 PsA patients, fulfilling CASPAR criteria, underwent detailed sk...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2017-03-01 00:00:00
abstract：:Patients with rheumatoid arthritis are at greater risk of infectious morbidity and mortality due to disease-related abnormalities and use of immunosuppressive medications. Vaccinations are recommended by international guidelines among infection control strategies, but vaccination rates are reported to be still subopti...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVES:To develop a standardized scoring system to assess the severity of DUs in SSc patients and correlate it with functional outcomes. METHODS:In this cross-sectional, longitudinal study in SSc patients with DUs (n=65) we developed a digital ulcers score (DUS) for the assessment of DUs. DUS and the ABILHAND scor...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：:Although osteoarthritis (OA) was historically referred to as the non-inflammatory arthritis, it is now considered a condition involving persistent low-grade inflammation and activation of innate inflammatory pathways. Synovitis increases the risk of OA onset and progression and involves the recruitment of monocytes, l...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2019-09-01 00:00:00
