Abstract：
OBJECTIVES:To determine the protein expression of TNFAIP3 in synovium and to show the capability of 6q23 intergenic SNPs, associated with rheumatoid arthritis (RA) susceptibility, to influence TNFAIP3 gene transcription. METHODS:Immunohistochemistry for TNFAIP3, NF-kB p65 and phosphorylated NF-kB p65 protein expression was performed in 6 RA knee joint synovium samples compared to 9 osteoarthritis (OA) samples. Luciferase reporter gene assays were used to examine the regulatory ability of RA associated SNP variants on TNFAIP3 promoter activity. Sense and antisense constructs were prepared for rs6920220 alleles, together with each of the 4 SNPs in r2=1 with it (rs6933404, rs2327832, rs6927172 and rs17264332), coupled to the TNFAIP3 promoter. Transient transfections were performed in a human T lymphoblastoid (CEMC7A) cell line. Bioinformatic software was utilised to prioritise SNPs for further investigation. Electrophoretic mobility shift assays (EMSA), using CEMC7A nuclear extracts, were conducted for the rs6927172 SNP alleles. RESULTS:TNFAIP3 protein expression was seen in the synovium samples and differential TNFAIP3 protein expression between RA vs. OA synoviocytes observed. Within RA synoviocytes TNFAIP3 expression is predominately cytoplasmic, whereas in OA its expression is strongly nuclear and cytoplasmic. For 3 of the 5 SNPs investigated (rs6920220, rs6933404, rs6927172) evidence of repressor activity of TNFAIP3 transcription was seen and EMSA data showed evidence of differential transcription factor binding to rs6927172 alleles. CONCLUSIONS:This is the first observation of TNFAIP3 protein expression in RA and OA synovium. In vitro analysis of 6q23 intergenic SNPs supports the possibility of the functional regulation of TNFAIP3.
journal_name
Clin Exp Rheumatoljournal_title
Clinical and experimental rheumatologyauthors
Elsby LM,Orozco G,Denton J,Worthington J,Ray DW,Donn RPsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
708-14issue
5eissn
0392-856Xissn
1593-098Xpii
916journal_volume
28pub_type
杂志文章abstract：OBJECTIVES:Fibromyalgia (FM) is a form of non-articular rheumatism characterised by chronic widespread musculoskeletal aching. Although some works have investigated the possible role of oxidative stress in the pathophysiology of FM, none has analysed a significant number of oxidative markers in the same patients. Conse...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:This review considers the epidemiological aspects of the genetic investigation of rheumatoid arthritis (RA). Problems both of disease definition and disease heterogeneity render studies difficult to undertake and interpret. The selection of individuals both in population and family studies can explain divergent result...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：1992-05-01 00:00:00
abstract：OBJECTIVE:To determine serum interleukin-10 (IL-10) levels in systemic lupus erythematosus (SLE) patients and to assess their relationship with disease activity. METHODS:Forty-one SLE patients and 35 controls were studied. Paired serum samples were collected from all SLE patients at the time of their presentation with...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：PURPOSE AND METHODS:In order to evaluate the prevalence of positive hepatitis C virus (HCV) serology and cryoglobulinemia in human immunodeficiency virus (HIV)-infected patients, the prevalence and the clinical significance of cryoglobulinemia were prospectively studied in a cohort of 86 HIV-infected subjects seen as o...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the relationship between rheumatoid arthritis (RA) and the occurrence of diabetes mellitus (DM). METHODS:A meta-analysis was conducted to explore the risk of DM in RA patients. All relevant studies were identified by searching PUBMED, EMBASE and MEDLINE database prio...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,meta分析,评审
doi：
更新日期：2015-01-01 00:00:00
abstract：:Skin inflammation is a physiological reaction to tissue injury, pathogen invasion and irritants. During this process, innate and/or adaptive immune cells are activated and recruited to the site of inflammation to either promote or suppress inflammation. The sequential recruitment and activation of immune cells is modu...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2015-07-01 00:00:00
abstract：:To evaluate the properties of antibodies to bacterial DNA in the sera of normal human subjects (NHS) and patients with systemic lupus erythematosus (SLE), the effects of ionic strength and pH on their binding to single-stranded DNA (ssDNA) from Micrococcus lysodeikticus (MC) were measured. By ELISA, antibodies to MC s...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：:A 57-year-old female patient presented with a tenosynovitis due to Mycobacterium avium-intracellulare (MAI). A relapse occurred following surgical treatment, after which she was successfully treated with ansamycin and ethambutol. Previously reported cases of tenosynovitis due to MAI are reviewed and the treatment moda...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：1992-03-01 00:00:00
abstract：OBJECTIVE:To determine the urinary levels of soluble vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in patients with systemic lupus erythematosus (SLE) and to assess their relationship with clinical and laboratory features and the degree of activity and damage associated with ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:Anti-TNF-α therapy has successfully been used to treat Takayasu arteritis (TA) refractory to conventional immunosuppressive treatment. However, some patients fail to respond even to TNF-α blockers. Interleukin-6 (IL-6) is a key player in the pathogenesis of TA. Preliminary data also suggest efficacy of the IL-6 recept...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:The association of malignancy with scleroderma is very rare in childhood. A 13-year-old girl was diagnosed as having thymic carcinoma and received systemic chemotherapy. She presented with symptoms of Raynaud's phenomenon 9 months after the cessation of chemotherapy. She also had difficulty in swallowing. Based on the...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:A case of acute neurologic deficit accompanied by a cerebral angiogram consistent with CNS vasculitis is presented. The differential diagnosis and diagnostic decision process generated in this type of evaluation is illustrated. ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:Twenty-three per cent of rheumatoid arthritis (RA) patients show an increase of serum IgA concentrations. To determine the role of mucous-associated lymphoid tissue (MALT) in the elevation of serum IgA in RA, we studied the serum secretory-IgA (s-IgA) in 63 RA patients and in 30 healthy controls. We also analysed the ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：OBJECTIVE:To answer and comment on a number of controversial issues in relation to osteoporosis and rheumatoid arthritis (RA), including: Is osteoporosis an extra-articular manifestation of rheumatoid arthritis? Does periarticular osteoporosis reflect disease activity in early arthritis? Is there a threshold for cortic...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：1995-09-01 00:00:00
abstract：OBJECTIVES:We estimated the cumulative patient survival rates, the causes of death and the initial predictors of death in Korean patients with microscopic polyangiitis (MPA), granulomatosis with polyangiitis (GPA) and eosinophilic GPA (EGPA). METHODS:We reviewed the medical records of 153 patients with antineutrophil ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVES:The health problems of patients with rheumatoid arthritis and fibromyalgia are mostly invisible to others, which can lead to a discrepancy between patients' and spouses' appraisals of the severity of the health problems. As a consequence, some patients may feel 'invalidation' from their spouse, such as not b...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：:Giant cell arteritis (GCA) and polymyalgia rheumatica (PMR) are closely related disorders that affect people of middle age and older, and frequently occur together. With the widespread use of newer vascular imaging modalities, large-vessel involvement (LVI) has increasingly been recognised in patients with GCA and les...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVES:To evaluate basal serum prolactin (PRL) levels in systemic sclerosis (SSc) patients with different degrees of skin involvement, and investigate its relationship with some of the clinical and serological parameters of the disease. METHODS:Basal serum PRL was measured in 44 SSc patients (38 F, 6 M) using a ra...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:MALT, or mucosa associated lymphoid tissue, is normally not present in gastric tissue. Its presence is often associated with persistent antigenic stimulation. MALT is a precursor of gastric MALT lymphoma, a low-grade lymphoma whose incidence recently appears to have increased. Although much epidemiologic and clinical ...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：OBJECTIVE:To determine the value of serial measurements of circulating cytokines in patients with rheumatoid arthritis in response to the introduction of disease modifying anti-rheumatic drugs (DMARDs). METHODS:A prospective 12-week study of 98 patients starting second line therapy with serial measurements of IL1 beta...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：OBJECTIVE:Behçet's disease (BD) is a unique systemic vasculitis involving both arteries and veins of all sizes. Since Fcgamma receptors (FcgammaR) are important in mediating various immune effector functions, FcgammaR gene polymorphisms may affect the susceptibility to systemic inflammatory diseases such as BD. The aim...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：OBJECTIVE:Blood cortisol and dehydroepiandrosterone sulphate (DHEAS) levels, and CD4 T cell counts were assessed in a group of 44 patients with HIV infection (17 asymptomatic and 27 symptomatic). The steroid levels were compared to those in 80 healthy subjects. RESULTS:The mean cortisol level did not differ between th...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：OBJECTIVES:Antibodies directed to citrullinated proteins are highly specific for rheumatoid arthritis (RA). Citrullination is catalyzed by peptidylarginine deiminase (PAD) enzymes. The current study examined the mRNA expression of PADI2 and PADI4 in bone marrow (BM) CD34+ cells from RA patients. METHODS:CD34+ cells we...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
abstract：:The knee is a frequent target for gout and calcium pyrophosphate dihydrate (CPPD) disease with involvement of both articular and peri-articular structures. The aims of the present study were to investigate the relationship between clinical and ultrasound (US) findings and to describe the prevalence and distribution of...
journal_title：Clinical and experimental rheumatology
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVES:The aim of the present study was to assess the effectiveness of belly dance as a treatment option for patients with fibromyalgia. METHODS:Eighty female patients with fibromyalgia between 18 to 65 years were randomly allocated to a dance group (n=40) and control group (n=40). Patients in the dance group unde...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2012-11-01 00:00:00
abstract：:We report a patient with Behçet's syndrome who presented with upper gastrointestinal haemorrhage. Gastroduodenoscopy showed a gastroduodenal fistula which caused the appearance of double pylorus in the antrum. The possibility of peptic ulcer disease related to non-steroidal anti-inflammatory drug use or Behçet's syndr...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：:The definition of remission in patients with systemic vasculitis must be distinguished from the term "cure," which implies that patients are well and not requiring ongoing therapy. Remission should be defined using a standardised approach to measuring clinical disease activity, and the definition should be qualified b...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章,评审
doi：
更新日期：2006-11-01 00:00:00
abstract：OBJECTIVES:To assess increased sacroiliac joint (SIJ) uptake on 18F-NaF PET/CT according to a qualitative and quantitative approach and to compare with MRI SIJ assessments for structural and inflammatory sacroiliitis in a population of 23 patients with spondyloarthritis (SpA) (IDRCB: 2012-A00568-35; ClinicalTrials.gov:...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVES:To investigate serum levels of endostatin in a well characterised cohort of patients with primary Sjögren's syndrome (pSS) and healthy controls (HC) and assess associations between these mediators and clinical parameters. METHODS:All patients (n=144) were recruited from the Norwegian systemic connective tis...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVES:To evaluate the clinical characteristics and imaging results (CDS, 18-FDG-PET) of patients with large vessel giant cell arteritis (LV-GCA) presenting as fever of unknown origin (FUO). METHODS:From a series of 82 patients with GCA we identified 8 patients with FUO as initial disease manifestation. Clinical c...
journal_title：Clinical and experimental rheumatology
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
