Identification of Malassezia species in patients with seborrheic dermatitis in China.

Abstract:

BACKGROUND:The causes of seborrheic dermatitis (SD) are complex and incompletely understood. Among the factors, Malassezia yeasts have been reported to play a major etiological role in SD. Many previous studies adopted conventional culture methods that were disadvantaged to detect Malassezia microflora in SD patients, resulting in a low detection rate for each species and high variance in types of microflora observed. OBJECTIVE:This study analyzed Malassezia microflora in SD patients by applying a transparent dressing to the lesional skin and using direct detection of fungal DNA using nested PCR. METHODS:We collected samples from the lesional skin of 146 SD patients in China and extracted fungal DNA directly from the lesional samples without culture. Specific primers for each Malassezia species were designed to amplify existing yeasts in each sample. Some samples were randomly selected to culture and identified by morphological and physiologic criteria. RESULTS:M. globosa and M. restricta were found in 87.0 and 81.5% of seborrheic dermatitis patients, respectively, which together accounted for more than 50% of Malassezia spp. recovered in these Chinese patients. The majority of SD patients (82.9%) showed co-colonization of two or more Malassezia species. CONCLUSION:M. globosa and M. restricta predominated in Malassezia colonization in Chinese SD patients. Compared with conventional culture, non-culture-based methods may more accurately reflect Malassezia microflora constitution.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Zhang H,Ran Y,Xie Z,Zhang R

doi

10.1007/s11046-012-9606-z

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

83-9

issue

1-2

eissn

0301-486X

issn

1573-0832

journal_volume

175

pub_type

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