Abstract：
:Hybrid dendritic-linear block copolymers based on a 4-arm poly(ethylene glycol) (PEG) core were synthesized using an accelerated AB2/CD2 dendritic growth approach through orthogonal amine/epoxy and thiol-yne chemistries. The biological activity of these 4-arm and the corresponding 2-arm hybrid dendrimers revealed an enhanced, dendritic effect with an exponential increase in cell internalization concomitant with increasing amine end groups and low cytotoxicity. Furthermore, the ability of these hybrid dendrimers to induce endosomal escape combined with their facile and efficient synthesis makes them attractive platforms for gene transfection. The 4-arm-based dendrimer showed significantly improved DNA binding and gene transfection capabilities in comparison with the 2-arm derivative. These results combined with the MD simulation indicate a significant effect of both the topology of the PEG core and the multivalency of these hybrid macromolecules on their DNA binding and delivery capablities.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Albertazzi L,Mickler FM,Pavan GM,Salomone F,Bardi G,Panniello M,Amir E,Kang T,Killops KL,Bräuchle C,Amir RJ,Hawker CJdoi
10.1021/bm301384ysubject
Has Abstractpub_date
2012-12-10 00:00:00pages
4089-97issue
12eissn
1525-7797issn
1526-4602journal_volume
13pub_type
杂志文章abstract：:A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the side chain could inhibit ice growth....
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801069x
更新日期：2009-02-09 00:00:00
abstract：:Algae constitute a largely available, low value material from renewable resources of marine origin to be used for the production of eco-compatible composites. Fibers of the green alga Ulva armoricana from the French coast were positively evaluated for the production of composites with a hydrophilic, eco-compatible pol...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701041e
更新日期：2008-03-01 00:00:00
abstract：:Polymeric prodrugs of mPEG-PLA-haloperidol (methoxypoly(ethylene glycol)-b-poly(lactic acid)) can self-assemble into nanoscale micelle-like structures in aqueous solutions. mPEG-PLA-haloperidol was prepared and characterized using 1H and 13C NMR. The conjugation efficiency was found to be 64.8 +/- 21%. Micelles that f...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050188k
更新日期：2005-09-01 00:00:00
abstract：:An appropriate modification technique for silk materials is needed to effectively improve their physical properties for specific applications. A telechelic-type polyalanine (T-polyA) was synthesized by papain-catalyzed polymerization as a novel reinforcing agent for silk materials. A silk fibroin obtained from Bombyx ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01891
更新日期：2017-03-13 00:00:00
abstract：:Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801430x
更新日期：2009-05-11 00:00:00
abstract：:Single-walled carbon nanotube (SWNT) is a pseudo-one-dimensional nanostructure capable of carrying/displaying a large number of bioactive molecules and species in aqueous solution. In this work, a series of dendritic beta-D-galactopyranosides and alpha-D-mannopyranosides with a terminal amino group were synthesized an...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800395e
更新日期：2008-09-01 00:00:00
abstract：:Turbidimetric titration was used to initiate associative intermolecular interactions between a pair of protein molecules, gelatin-A and gelatin-B, having complementary charges that led to pH-induced liquid-liquid phase separation and the formation of complex coacervate. The stoichiometric binding ratio was found to be...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801160s
更新日期：2009-01-12 00:00:00
abstract：:Spider dragline silk exhibits an extraordinary toughness and is typically composed of two types of major ampullate spidroins (MaSp1 and MaSp2), differing in their proline content and hydrophobicity. In this paper, we recombinantly produced an unusual but naturally occurring short major ampullate spidroin (MaSp1s) as a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00090
更新日期：2017-04-10 00:00:00
abstract：:Eosinophil cationic protein (ECP) is an antimicrobial protein belonging to the superfamily of RNase A. ECP exhibits a broad spectrum of action against bacteria and, at higher concentrations, displays cytotoxic activity to eukaryotic cells. Recently, a powerful aggregation activity for lipid vesicles and for the gram-n...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100334u
更新日期：2010-08-09 00:00:00
abstract：:The ability of small interfering RNA (siRNA) to efficiently silence the expression of specific genes provides the basis for exciting new therapies based on RNA interference (RNAi). The efficient intracellular delivery of siRNA from cell uptake through the endosomal trafficking pathways into the cytoplasm remains a sig...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100652w
更新日期：2010-11-08 00:00:00
abstract：:Physicochemical properties of a homogeneous series of chitosans with different degrees of acetylation and almost the same degree of polymerization were investigated in an ammonium acetate buffer. Techniques such as interferometry, static light scattering (in batch or coupled on line with a chromatographic system), and...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025724c
更新日期：2003-05-01 00:00:00
abstract：:There is a lack of hydrogel materials whose properties can be tuned at the point of use. Biological hydrogels, such as collagen, gelate at physiological temperatures; however, they are not always ideal as scaffolds because of their low mechanical strength. Their mechanics can be improved through cross-linking and chem...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01251
更新日期：2016-03-14 00:00:00
abstract：:Oral delivery remains a challenge for poorly permeable hydrophilic macromolecules. Poly(amido amine) (PAMAM) dendrimers have shown potential for their possible oral delivery. Transepithelial transport of carboxyl-terminated G3.5 and amine-terminated G4 PAMAM dendrimers was assessed using isolated rat jejunal mucosae m...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5004465
更新日期：2014-08-11 00:00:00
abstract：:In the present study, novel bionanocomposite materials with tunable properties were successfully prepared using a poly(lactic acid) (PLA) matrix and acetylated microfibrillated cellulose (MFC) as reinforcing agent. The acetylation of MFC was confirmed by FTIR and (13)C CP-MAS NMR spectroscopies. The grafting of acetyl...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm901186u
更新日期：2010-02-08 00:00:00
abstract：:Most biopolymers exist in a plasticized state, whether it is naturally with water or unnaturally with glycerol or other suitable polyol, to make a flexible material. We have found that the extent to which a biopolymer can be plasticized is dependent on its molecular and higher order structures outside of simply molecu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800759g
更新日期：2008-11-01 00:00:00
abstract：:Stimuli-responsive hydrogels have great potentials in biomedical and biotechnological applications. Due to the advantages of precise control over molecular weight and being biodegradable, protein-based hydrogels and their applications have been extensively studied. However, protein hydrogels with dual thermosensitive ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01231
更新日期：2015-11-09 00:00:00
abstract：:In this work, 6-phosphogluconic trisodium salt (6-PG-Na+) is introduced as a new aqueous and nontoxic cross-linking agent to obtain ionic hydrogels. Here, it is shown the formation of hydrogels based on chitosan cross-linked with 6-PG-Na+. This formulation is obtained by ionic interaction of cationic groups of polymer...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00108
更新日期：2018-04-09 00:00:00
abstract：:Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) was applied to investigate the interaction of bovine serum albumin (BSA) and fibrinogen with a biomedical-grade 316LVM stainless steel surface, in terms of the adsorption thermodynamics and adsorption-induced secondary structure changes of ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm070289d
更新日期：2007-09-01 00:00:00
abstract：:Hydrogels are able to mimic the basic three-dimensional (3D) biological, chemical, and mechanical properties of native tissues. Since hyaluronic acid (HA) is a chief component of human extracellular matrix (ECM), it represents an extremely attractive starting material for the fabrication of scaffolds for tissue engine...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401712q
更新日期：2014-02-10 00:00:00
abstract：:In this study, generation 5 (G5) polyamidoamine (PAMAM) dendrimers with two different surface groups, G4.5-COOH and G5-OH, were investigated for their protective effects on pancreas injury in a caerulein-induced acute pancreatitis (AP) mouse model. Both dendrimers significantly decreased pathological changes in the pa...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm501390d
更新日期：2015-01-12 00:00:00
abstract：:A novel methacrylate monomer containing a quinolone moiety was synthesized and homopolymerized in N,N-dimethylformamide (DMF) by using azobisisobutyronitrile (AIBN) as an initiator. The new monomer was copolymerized with poly(ethylene glycol) methyl ether methacrylate (MPEGMA) in DMF using the same initiator. The mono...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049383+
更新日期：2005-01-01 00:00:00
abstract：:We report the facile preparation of gels from the hydrothermal treatment of suspensions of cellulose nanocrystals (CNCs). The properties of the hydrogels have been investigated by rheology, electron microscopy, and spectroscopy with respect to variation in the temperature, time, and CNC concentration used in preparati...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00906
更新日期：2016-08-08 00:00:00
abstract：:Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modificati...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00470
更新日期：2015-07-13 00:00:00
abstract：:A novel pH and temperature sensitive block copolymer was prepared by adding pH sensitive moiety to temperature sensitive block copolymer. This block copolymer solution showed a reversible sol-gel transition by a small pH change in the range of pH 7.4-8.0 and also by the temperature change in the region of body tempera...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050521k
更新日期：2005-11-01 00:00:00
abstract：:The importance of the stabilization of the quaternary structure of multimeric enzymes has been illustrated using a model reaction with great industrial relevance: the enzymatic synthesis of ampicillin from 6-amino penicillanic acid (6APA) and phenylglycine methyl ester (PGM) catalyzed by the tetrameric enzyme alpha-am...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm000072i
更新日期：2001-04-01 00:00:00
abstract：:Spherulites of poly(L-lactic acid) (PLLA) and of its blends with atactic poly(3-hydroxybutyrate) (a-PHB, from 10 to 75 wt %) were investigated by microfocus X-ray diffraction using synchrotron radiation. Radial scans in 5 microm steps with 3 microm beam diameter were performed. In PLLA, tens of identical diffraction i...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0343951
更新日期：2004-03-01 00:00:00
abstract：:Aberrant expression of histone deacetylases (HDACs) is related to various types of cancer and is associated with increased proliferation of tumor cells. Hence, the detection of HDAC activities is of great significance for medical sciences as well as biological diagnostics. Herein, we report a hyperbranched polyester-b...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401548u
更新日期：2013-12-09 00:00:00
abstract：:Electrospun biopolyelectrolyte nanofibers hold potential for use in a range of biomedical applications, but eliminating toxic chemicals involved in their production remains a key challenge. In this study, we successfully electrospun nanofibers from an aqueous complex coacervate solution composed of chitosan and hyalur...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01072
更新日期：2019-11-11 00:00:00
abstract：:The encapsulation of live cells with polymeric coat-ings is a versatile approach to modulate or control the response cells to their environment. The layer-by-layer (LbL) self-assembly of nonimmunogenic polyelectrolytes is employed here to attenuate or suppress the binding of antibodies to live red blood cells (RBCs) a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm101200c
更新日期：2011-03-14 00:00:00
abstract：:Water-soluble, degradable polymers based on poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) with low cytotoxicity and good p-DNA transfection efficiency are highlighted in this article. To solve the nondegradability issue of PDMAEMA, new polymers based on DMAEMA and 5,6-benzo-2-methylene-1,3-dioxepane (BMDO) for g...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2015174
更新日期：2012-02-13 00:00:00
