Culture-dependent and independent studies of microbial diversity in highly copper-contaminated Chilean marine sediments.

Abstract:

:Cultivation and molecular-based approaches were used to study microbial diversity in two Chilean marine sediments contaminated with high (835 ppm) and very high concentrations of copper (1,533 ppm). The diversity of cultivable bacteria resistant to copper was studied at oxic and anoxic conditions, focusing on sulfate-, thiosulfate-, and iron-reducing bacteria. For both sediments, the cultivable bacteria isolated at oxic conditions were mostly affiliated to the genus Bacillus, while at anoxic conditions the majority of the cultivable bacteria found were closely related to members of the genera Desulfovibrio, Sphingomonas, and Virgibacillus. Copper resistance was between 100 and 400 ppm, with the exception of a strain affiliated to members of the genus Desulfuromonas, which was resistant up to 1,000 ppm of copper. In parallel, cloning and sequencing of 16S rRNA was performed to study the total bacterial diversity in the sediments. A weak correlation was observed between the isolated strains and the 16S rRNA operational taxonomic units detected. The presence of copper resistance genes (copA, cusA, and pcoA) was tested for all the strains isolated; only copA was detected in a few isolates, suggesting that other copper resistance mechanisms could be used by the bacteria in those highly copper-contaminated sediments.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Besaury L,Marty F,Buquet S,Mesnage V,Muyzer G,Quillet L

doi

10.1007/s00248-012-0120-0

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

311-24

issue

2

eissn

0095-3628

issn

1432-184X

journal_volume

65

pub_type

杂志文章
  • Transformation of chlorinated phenolic compounds in the genusRhodococcus.

    abstract::The ability of strains of the genusRhodococcus to transform chlorinated phenolic compounds was studied. Noninduced cells of several strains ofRhodococcus, covering at least eight species, were found to attack mono-, di-, and trichlorophenols by hydroxylation at theortho position to chlorocatechols. 3-chlorophenol and ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02030123

    authors: Häggblom MM,Janke D,Salkinoja-Salonen MS

    更新日期:1989-09-01 00:00:00

  • Shifts in microbial community composition following surface application of dredged river sediments.

    abstract::Sediment input to the Illinois River has drastically decreased river depth and reduced habitats for aquatic organisms. Dredging is being used to remove sediment from the Illinois River, and the dredged sediment is being applied to the surface of a brownfield site in Chicago with the goal of revegetating the site. In o...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-008-9410-y

    authors: Baniulyte D,Favila E,Kelly JJ

    更新日期:2009-01-01 00:00:00

  • Effect of culture media onLactobacillus hydrophobicity and electrophoretic mobility.

    abstract::The hydrophobicity of six strains representing three species ofLactobacillus was measured using dextran-polyethylene glycol contact angle measurements. These ranged from 123.6° forLactobacillus casei douche to 26.2° forL. casei RC-17 under identical growth conditions. The results indicated that the nutritional environ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02025590

    authors: Eisen A,Reid G

    更新日期:1989-01-01 00:00:00

  • Environmental Drivers of Microbial Functioning in Mediterranean Forest Soils.

    abstract::Mediterranean forests own distinct characteristics resulting from climate, soil, and vegetation that affect soil microbial communities' assembly and their associated functions. We initiated a multi-scalar analysis of environmental drivers of soil functioning to (1) identify pertinent factorial scales and (2) determine...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-020-01518-5

    authors: Brunel C,Da Silva AF,Gros R

    更新日期:2020-10-01 00:00:00

  • Diversity of assimilatory nitrate reductase genes from plankton and epiphytes associated with a seagrass bed.

    abstract::Assimilatory nitrate reductase gene fragments were isolated from epiphytes and plankton associated with seagrass blades collected from Tampa Bay, Florida, USA. Nitrate reductase genes from diatoms (NR) and heterotrophic bacteria (nasA) were amplified by polymerase chain reaction (PCR) using two sets of degenerate prim...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-006-9175-0

    authors: Adhitya A,Thomas FI,Ward BB

    更新日期:2007-11-01 00:00:00

  • Geographical Patterns of nirS Gene Abundance and nirS-Type Denitrifying Bacterial Community Associated with Activated Sludge from Different Wastewater Treatment Plants.

    abstract::Denitrifying bacteria is a driver of nitrogen removal process in wastewater treatment ecosystem. However, the geographical characteristics of denitrifying bacterial communities associated with activated sludge from diverse wastewater treatment plants (WWTPs) are still unclear. Here, quantitative PCR and next-generatio...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1236-7

    authors: Zhang H,Feng J,Chen S,Zhao Z,Li B,Wang Y,Jia J,Li S,Wang Y,Yan M,Lu K,Hao H

    更新日期:2019-02-01 00:00:00

  • The establishment of an intestinal microflora in developing goldfish (Carassius auratus) of culture ponds.

    abstract::The bacterial flora in the intestinal tract of goldfish (Carassius auratus) was investigated at different stages of fish development. The floras of the diets and the water and sediment of a culture pond were also analyzed. The total counts in the intestine ranged from 2.2 × 10(6)-2.1 × 10(8) cells g(-1) wet weight.Aer...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02012646

    authors: Sugita H,Tsunohara M,Ohkoshi T,Deguchi Y

    更新日期:1988-05-01 00:00:00

  • A Comparison of the Survival of Intraperiplasmic and Attack Phase Bdellovibrios with Reduced Oxygen

    abstract::The ability of intraperiplasmic and attack phase bdellovibrios to survive and/or grow under anoxic and microaerobic conditions was examined. Both halotolerant and nonhalotolerant bdellovibrio strains were examined. In all instances, the bdellovibrio strains were unable to grow under anoxic conditions, but were able to...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00170105

    authors: Schoeffield AJ,Williams HN,Turng B,Fackler WA Jr

    更新日期:1996-07-01 00:00:00

  • Ecology of bacterial communities in the schistosomiasis vector snailBiomphalaria glabrata.

    abstract::The internal colony-forming bacterial flora of the schistosome intermediate host snailBiomphalaria glabrata (Say) has been characterized in ca. 500 individual snails from Puerto Rico, Guadeloupe, and St. Lucia, and from laboratory aquaria. Freshly captured wild snails harbor 2-40×10(6) CFU·g(-1), and healthy aquarium ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02010308

    authors: Ducklow HW,Clausen K,Mitchell R

    更新日期:1981-09-01 00:00:00

  • Bacterial community structure of sediments of the bizerte lagoon (Tunisia), a southern Mediterranean coastal anthropized lagoon.

    abstract::In order to estimate how pollution affects the bacterial community structure and composition of sediments, chemical and molecular approaches were combined to investigate eight stations around the Bizerte lagoon. Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified 16S rRNA genes reveale...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-009-9585-x

    authors: Ben Said O,Goñi-Urriza M,El Bour M,Aissa P,Duran R

    更新日期:2010-04-01 00:00:00

  • The significance of viruses to mortality in aquatic microbial communities.

    abstract::A variety of approaches including enumeration of visibly infected microbes, removal of viral particles, decay of viral infectivity, and measurements of viral production rates have been used to infer the impact of viruses on microbial mortality. The results are surprisingly consistent and suggest that, on average, abou...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00166813

    authors: Suttle CA

    更新日期:1994-09-01 00:00:00

  • Micrococcus luteus -- survival in amber.

    abstract::A growing body of evidence now supports the isolation of microorganisms from ancient materials. However, questions about the stringency of extraction methods and the genetic relatedness of isolated organisms to their closest living relatives continue to challenge the authenticity of these ancient life forms. Previous ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-003-2016-5

    authors: Greenblatt CL,Baum J,Klein BY,Nachshon S,Koltunov V,Cano RJ

    更新日期:2004-07-01 00:00:00

  • Species diversity, distribution, and genetic structure of endophytic and epiphytic Trichoderma associated with banana roots.

    abstract::Selective isolation, molecular identification and AFLP were used to investigate the distribution of the various species of endophytic and epiphytic Trichoderma associated with banana roots and to compare and contrast their genetic structure. Three specific groups of Trichoderma were observed in the roots of banana. Gr...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-010-9770-y

    authors: Xia X,Lie TK,Qian X,Zheng Z,Huang Y,Shen Y

    更新日期:2011-04-01 00:00:00

  • On describing microbial growth kinetics from continuous culture data: Some general considerations, observations, and concepts.

    abstract::Analysis of continuous culture methodology suggests that this potentially powerful tool for kinetic analysis can be improved by minimizing several inherent shortcomings. Medium background substrates - organic carbon, phosphate, and manganese - were shown to dominate kinetic observations at concentrations below chemica...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02011647

    authors: Law AT,Robertson BR,Dunker SS,Button DK

    更新日期:1975-12-01 00:00:00

  • Microbiota Diversity Within and Between the Tissues of Two Wild Interbreeding Species.

    abstract::Understanding the role of microbiota as reproductive barriers or sources of adaptive novelty in the fundamental biological phenomenon of speciation is an exciting new challenge necessitating exploration of microbiota variation in wild interbreeding species. We focused on two interbreeding cyprinid species, Chondrostom...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-017-1077-9

    authors: Guivier E,Martin JF,Pech N,Ungaro A,Chappaz R,Gilles A

    更新日期:2018-04-01 00:00:00

  • Endogenous methanogenesis stimulates oxidation of atmospheric CH(4) in alpine tundra soil.

    abstract::Experiments were done to test the hypothesis that atmospheric CH(4) oxidizers in a well-drained alpine tundra soil are supported by CH(4) production from anaerobic microsites in the soil. Soil was subjected to 22 days of anaerobic conditions with elevated H(2) and CO(2) in order to stimulate methanogenesis. This treat...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-001-1049-x

    authors: West AE,Schimdt SK

    更新日期:2002-05-01 00:00:00

  • Plant Compartments and Developmental Stages Modulate the Balance between Niche-Based and Neutral Processes in Soybean Microbiome.

    abstract::Understanding the dynamics of plant-associated microbial communities within agriculture is well documented. However, the ecological processes that assemble the plant microbiome are not well understood. This study elucidates the relative dominance of assembly processes across plant compartments (root, stem, and leaves)...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-021-01688-w

    authors: Moroenyane I,Mendes L,Tremblay J,Tripathi B,Yergeau É

    更新日期:2021-01-18 00:00:00

  • PAH degradation capacity of soil microbial communities--does it depend on PAH exposure?

    abstract::Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants of the environment. But is their microbial degradation equally wide in distribution? We estimated the PAH degradation capacity of 13 soils ranging from pristine locations (total PAHs approximately 0.1 mg kg(-1)) to heavily polluted industrial sites (tot...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-005-0022-5

    authors: Johnsen AR,Karlson U

    更新日期:2005-11-01 00:00:00

  • Competition of two suspension-feeding protozoan populations for a growing bacterial population in continuous culture.

    abstract::Mathematical studies for ecosystems involving 2 predators competing for a growing prey population have shown that the 2 competitors can coexist in a state of sustained oscillations for a range of values of the system parameters. For the case of 1 suspension-feeding protozoan population, recent experimental observation...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02011595

    authors: Baltzis BC,Fredrickson AG

    更新日期:1984-03-01 00:00:00

  • Anomalies in the enumeration of starved bacteria on culture media containing nalidic acid and tetracycline.

    abstract::Culturable counts of antibiotic resistant, genetically engineeredPseudomonas fluorescens were determined on antibiotic-containing plate count agar during starvation in water. Prior to starvation, colony counts obtained on all media separated into two groups. The mean of the colony counts on plate count agar with or wi...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02543883

    authors: Genthner FJ,Upadhyay J,Campbell RP,Genthner BR

    更新日期:1990-12-01 00:00:00

  • Symbiont-Mediated Host-Parasite Dynamics in a Fungus-Gardening Ant.

    abstract::Group-living can promote the evolution of adaptive strategies to prevent and control disease. Fungus-gardening ants must cope with two sets of pathogens, those that afflict the ants themselves and those of their symbiotic fungal gardens. While much research has demonstrated the impact of specialized fungal pathogens t...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-017-1124-6

    authors: Kellner K,Kardish MR,Seal JN,Linksvayer TA,Mueller UG

    更新日期:2018-08-01 00:00:00

  • Influence of zinc, lead, and cadmium pollutants on the microflora of hawthorn leaves.

    abstract::Transect studies were conducted to determine the relative effects of zinc, lead, and cadmium pollution on microorganisms occurring on hawthorn leaves at varying distances from a smelting complex.Sporobolomyces roseus was absent from the most heavily contaminated leaves but, although lead was inhibitory, other environm...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02010388

    authors: Bewley RJ,Campbell R

    更新日期:1980-09-01 00:00:00

  • Effects of organic amendments on sulfate reduction activity, H2 consumption, and H 2 production in salt marsh sediments.

    abstract::Sulfate reduction activity (SRA) was measured via the radioactive tracer ((35)SO4 (=)) technique in sediment samples from the Canary Creek Marsh in Lewes, Delaware. Basal levels of SRA ranged from 130 to 319 nmoles of sulfate reduced/gram dry sediment/hour. With the exception of lactate and formate, all organic acids ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02016814

    authors: Dicker HJ,Smith DW

    更新日期:1985-12-01 00:00:00

  • The Combined Effect of Temperature and Host Clonal Line on the Microbiota of a Planktonic Crustacean.

    abstract::Host-associated microbiota vary across host individuals and environmental conditions, but the relative importance of their genetic background versus their environment is difficult to disentangle. We sought to experimentally determine the factors shaping the microbiota of the planktonic Crustacean, Daphnia magna. We us...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-017-1126-4

    authors: Sullam KE,Pichon S,Schaer TMM,Ebert D

    更新日期:2018-08-01 00:00:00

  • New insights into the ecological interaction between grape berry microorganisms and Drosophila flies during the development of sour rot.

    abstract::In this work, we studied the ecological interactions between grape berry microorganisms and Drosophila sp. flies involved in sour rot disease during grape ripening. After veráison the total microbial counts of grape berries affected by sour rot increased from about 2 log CFU/g of berries to more than 7 log CFU/g. Berr...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-012-0041-y

    authors: Barata A,Santos SC,Malfeito-Ferreira M,Loureiro V

    更新日期:2012-08-01 00:00:00

  • Microbial communities in the saturated groundwater environment I: Methods of isolation and characterization of heterotrophic bacteria.

    abstract::In this paper we present a method of isolation and morphological and physiological characterization of groundwater bacteria based on numerical taxonomy and cluster analysis, and using a miniaturized test system (microtiter plates). Bacteria were isolated randomly on P-agar, and each strain was characterized in regard ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02097402

    authors: Kölbel-Boelke J,Tienken B,Nehrkorn A

    更新日期:1988-07-01 00:00:00

  • Grazing of acidophilic bacteria by a flagellated protozoan.

    abstract::A biflagellated protozoan was isolated from an acidic drainage stream located inside a disused pyrite mine. The stream contained copious amounts of "acid streamer" bacterial growths, and the flagellate was observed in situ apparently grazing the streamer bacteria. The protozoan was obligately acidophilic, growing betw...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00165908

    authors: McGinness S,Johnson DB

    更新日期:1992-01-01 00:00:00

  • Experimental and modeling studies of a four-trophic level predator-prey system.

    abstract::Experimental studies of a microbial food chain involving organic carbon substrates,Enterobacter aerogenes, and ciliate protozoansParamecium primaurelia andDldinium nasutum were conducted in stirred, aerated batch cultures. Quantitative measurements were made of organic carbon levels and of cell numbers, mean cell volu...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02011426

    authors: Graham JM,Canale RP

    更新日期:1982-12-01 00:00:00

  • Migration of Paraburkholderia terrae BS001 Along Old Fungal Hyphae in Soil at Various pH Levels.

    abstract::The movement of bacterial cells along with fungal hyphae in soil (the mycosphere) has been reported in several previous studies. However, how local soil conditions affect bacterial migration direction in the mycosphere has not been extensively studied. Here, we investigated the influence of two soil parameters, pH and...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-017-1137-1

    authors: Yang P,Oliveira da Rocha Calixto R,van Elsas JD

    更新日期:2018-08-01 00:00:00

  • Epizoic communities of prokaryotes on healthy and diseased scleractinian corals in Lingayen Gulf, Philippines.

    abstract::In search for microbiological indicators of coral health and coral diseases, community profiles of coral-associated epizoic prokaryotes were investigated because of their dual potential as a source of coral pathogens and their antagonists. In pairwise samples of visually healthy and diseased coral specimens from Bolin...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-008-9400-0

    authors: Arboleda M,Reichardt W

    更新日期:2009-01-01 00:00:00