Real-time in vivo imaging of the beating mouse heart at microscopic resolution.

Abstract:

:Real-time imaging of moving organs and tissues at microscopic resolutions represents a major challenge in studying the complex biology of live animals. Here we present a technique based on a novel stabilizer setup combined with a gating acquisition algorithm for the imaging of a beating murine heart at the single-cell level. The method allows serial in vivo fluorescence imaging of the beating heart in live mice in both confocal and nonlinear modes over the course of several hours. We demonstrate the utility of this technique for in vivo optical sectioning and dual-channel time-lapse fluorescence imaging of cardiac ischaemia. The generic method could be adapted to other moving organs and thus broadly facilitate in vivo microscopic investigations.

journal_name

Nat Commun

journal_title

Nature communications

authors

Lee S,Vinegoni C,Feruglio PF,Fexon L,Gorbatov R,Pivoravov M,Sbarbati A,Nahrendorf M,Weissleder R

doi

10.1038/ncomms2060

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

1054

issn

2041-1723

pii

ncomms2060

journal_volume

3

pub_type

杂志文章
  • Free-space transfer of comb-rooted optical frequencies over an 18 km open-air link.

    abstract::Phase-coherent transfer of optical frequencies over a long distance is required for diverse photonic applications, including optical clock dissemination and physical constants measurement. Several demonstrations were made successfully over fiber networks, but not much work has been done yet through the open air where ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12443-8

    authors: Kang HJ,Yang J,Chun BJ,Jang H,Kim BS,Kim YJ,Kim SW

    更新日期:2019-09-30 00:00:00

  • Atomic scale insights into structure instability and decomposition pathway of methylammonium lead iodide perovskite.

    abstract::Organic-inorganic hybrid perovskites are promising candidates for the next-generation solar cells. Many efforts have been made to study their structures in the search for a better mechanistic understanding to guide the materials optimization. Here, we investigate the structure instability of the single-crystalline CH3...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-07177-y

    authors: Chen S,Zhang X,Zhao J,Zhang Y,Kong G,Li Q,Li N,Yu Y,Xu N,Zhang J,Liu K,Zhao Q,Cao J,Feng J,Li X,Qi J,Yu D,Li J,Gao P

    更新日期:2018-11-15 00:00:00

  • A fluoroxalate cathode material for potassium-ion batteries with ultra-long cyclability.

    abstract::Potassium-ion batteries are a compelling technology for large scale energy storage due to their low-cost and good rate performance. However, the development of potassium-ion batteries remains in its infancy, mainly hindered by the lack of suitable cathode materials. Here we show that a previously known frustrated magn...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-15044-y

    authors: Ji B,Yao W,Zheng Y,Kidkhunthod P,Zhou X,Tunmee S,Sattayaporn S,Cheng HM,He H,Tang Y

    更新日期:2020-03-06 00:00:00

  • Detection of spacer precursors formed in vivo during primed CRISPR adaptation.

    abstract::Type I CRISPR-Cas loci provide prokaryotes with a nucleic-acid-based adaptive immunity against foreign DNA. Immunity involves adaptation, the integration of ~30-bp DNA fragments, termed prespacers, into the CRISPR array as spacers, and interference, the targeted degradation of DNA containing a protospacer. Interferenc...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12417-w

    authors: Shiriaeva AA,Savitskaya E,Datsenko KA,Vvedenskaya IO,Fedorova I,Morozova N,Metlitskaya A,Sabantsev A,Nickels BE,Severinov K,Semenova E

    更新日期:2019-10-10 00:00:00

  • Unpredictable environments lead to the evolution of parental neglect in birds.

    abstract::A nest of begging chicks invites an intuitive explanation: needy chicks want to be fed and parents want to feed them. Surprisingly, however, in a quarter of species studied, parents ignore begging chicks. Furthermore, parents in some species even neglect smaller chicks that beg more, and preferentially feed the bigges...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms10985

    authors: Caro SM,Griffin AS,Hinde CA,West SA

    更新日期:2016-03-29 00:00:00

  • An earthquake gap south of Istanbul.

    abstract::Over the last century the North Anatolian Fault Zone in Turkey has produced a remarkable sequence of large earthquakes. These events have now left an earthquake gap south of Istanbul and beneath the Marmara Sea, a gap that has not been filled for 250 years. Here we investigate the nature of the eastern end of this gap...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms2999

    authors: Bohnhoff M,Bulut F,Dresen G,Malin PE,Eken T,Aktar M

    更新日期:2013-01-01 00:00:00

  • Single-cell RNA landscape of intratumoral heterogeneity and immunosuppressive microenvironment in advanced osteosarcoma.

    abstract::Osteosarcoma is the most frequent primary bone tumor with poor prognosis. Through RNA-sequencing of 100,987 individual cells from 7 primary, 2 recurrent, and 2 lung metastatic osteosarcoma lesions, 11 major cell clusters are identified based on unbiased clustering of gene expression profiles and canonical markers. The...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-20059-6

    authors: Zhou Y,Yang D,Yang Q,Lv X,Huang W,Zhou Z,Wang Y,Zhang Z,Yuan T,Ding X,Tang L,Zhang J,Yin J,Huang Y,Yu W,Wang Y,Zhou C,Su Y,He A,Sun Y,Shen Z,Qian B,Meng W,Fei J,Yao Y,Pan X,Chen P,Hu H

    更新日期:2020-12-10 00:00:00

  • A rapid cosmic-ray increase in BC 3372-3371 from ancient buried tree rings in China.

    abstract::Cosmic rays interact with the Earth's atmosphere to produce 14C, which can be absorbed by trees. Therefore, rapid increases of 14C in tree rings can be used to probe previous cosmic-ray events. By this method, three 14C rapidly increasing events have been found. Plausible causes of these events include large solar pro...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-01698-8

    authors: Wang FY,Yu H,Zou YC,Dai ZG,Cheng KS

    更新日期:2017-11-14 00:00:00

  • MyoD induced enhancer RNA interacts with hnRNPL to activate target gene transcription during myogenic differentiation.

    abstract::Emerging evidence supports roles of enhancer RNAs (eRNAs) in regulating target gene. Here, we study eRNA regulation and function during skeletal myoblast differentiation. We provide a panoramic view of enhancer transcription and categorization of eRNAs. Master transcription factor MyoD is crucial in activating eRNA pr...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-13598-0

    authors: Zhao Y,Zhou J,He L,Li Y,Yuan J,Sun K,Chen X,Bao X,Esteban MA,Sun H,Wang H

    更新日期:2019-12-19 00:00:00

  • Bactericidal activity of black silicon.

    abstract::Black silicon is a synthetic nanomaterial that contains high aspect ratio nanoprotrusions on its surface, produced through a simple reactive-ion etching technique for use in photovoltaic applications. Surfaces with high aspect-ratio nanofeatures are also common in the natural world, for example, the wings of the drago...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms3838

    authors: Ivanova EP,Hasan J,Webb HK,Gervinskas G,Juodkazis S,Truong VK,Wu AH,Lamb RN,Baulin VA,Watson GS,Watson JA,Mainwaring DE,Crawford RJ

    更新日期:2013-01-01 00:00:00

  • Powerful decomposition of complex traits in a diploid model.

    abstract::Explaining trait differences between individuals is a core and challenging aim of life sciences. Here, we introduce a powerful framework for complete decomposition of trait variation into its underlying genetic causes in diploid model organisms. We sequence and systematically pair the recombinant gametes of two interc...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms13311

    authors: Hallin J,Märtens K,Young AI,Zackrisson M,Salinas F,Parts L,Warringer J,Liti G

    更新日期:2016-11-02 00:00:00

  • MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage.

    abstract::Homologous recombination (HR) mediates the error-free repair of DNA double-strand breaks to maintain genomic stability. Here we characterize C17orf53/MCM8IP, an OB-fold containing protein that binds ssDNA, as a DNA repair factor involved in HR. MCM8IP-deficient cells exhibit HR defects, especially in long-tract gene c...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-16718-3

    authors: Huang JW,Acharya A,Taglialatela A,Nambiar TS,Cuella-Martin R,Leuzzi G,Hayward SB,Joseph SA,Brunette GJ,Anand R,Soni RK,Clark NL,Bernstein KA,Cejka P,Ciccia A

    更新日期:2020-06-11 00:00:00

  • Spatiotemporal dynamics of information encoding revealed in orbitofrontal high-gamma.

    abstract::High-gamma signals mirror the tuning and temporal profiles of neurons near a recording electrode in sensory and motor areas. These frequencies appear to aggregate local neuronal activity, but it is unclear how this relationship affects information encoding in high-gamma activity (HGA) in cortical areas where neurons a...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-01253-5

    authors: Rich EL,Wallis JD

    更新日期:2017-10-26 00:00:00

  • Modular coherent photonic-aided payload receiver for communications satellites.

    abstract::Ubiquitous satellite communications are in a leading position for bridging the digital divide. Fulfilling such a mission will require satellite services on par with fibre services, both in bandwidth and cost. Achieving such a performance requires a new generation of communications payloads powered by large-scale proce...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-10077-4

    authors: Duarte VC,Prata JG,Ribeiro CF,Nogueira RN,Winzer G,Zimmermann L,Walker R,Clements S,Filipowicz M,Napierała M,Nasiłowski T,Crabb J,Kechagias M,Stampoulidis L,Anzalchi J,Drummond MV

    更新日期:2019-04-30 00:00:00

  • Crystallographic structure of a small molecule SIRT1 activator-enzyme complex.

    abstract::SIRT1, the founding member of the mammalian family of seven NAD(+)-dependent sirtuins, is composed of 747 amino acids forming a catalytic domain and extended N- and C-terminal regions. We report the design and characterization of an engineered human SIRT1 construct (mini-hSIRT1) containing the minimal structural eleme...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms8645

    authors: Dai H,Case AW,Riera TV,Considine T,Lee JE,Hamuro Y,Zhao H,Jiang Y,Sweitzer SM,Pietrak B,Schwartz B,Blum CA,Disch JS,Caldwell R,Szczepankiewicz B,Oalmann C,Yee Ng P,White BH,Casaubon R,Narayan R,Koppetsch K,Bourb

    更新日期:2015-07-02 00:00:00

  • Sensing the future of bio-informational engineering.

    abstract::The practices of synthetic biology are being integrated into 'multiscale' designs enabling two-way communication across organic and inorganic information substrates in biological, digital and cyber-physical system integrations. Novel applications of 'bio-informational' engineering will arise in environmental monitorin...

    journal_title:Nature communications

    pub_type: 杂志文章,评审

    doi:10.1038/s41467-020-20764-2

    authors: Dixon TA,Williams TC,Pretorius IS

    更新日期:2021-01-15 00:00:00

  • Double thermoelectric power factor of a 2D electron system.

    abstract::Two-dimensional electron systems have attracted attention as thermoelectric materials, which can directly convert waste heat into electricity. It has been theoretically predicted that thermoelectric power factor can be largely enhanced when the two-dimensional electron layer is far narrower than the de Broglie wavelen...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-04660-4

    authors: Zhang Y,Feng B,Hayashi H,Chang CP,Sheu YM,Tanaka I,Ikuhara Y,Ohta H

    更新日期:2018-06-20 00:00:00

  • DNA-enabled rational design of fluorescence-Raman bimodal nanoprobes for cancer imaging and therapy.

    abstract::Recently, surface-enhanced Raman scattering nanoprobes have shown tremendous potential in oncological imaging owing to the high sensitivity and specificity of their fingerprint-like spectra. As current Raman scanners rely on a slow, point-by-point spectrum acquisition, there is an unmet need for faster imaging to cove...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09173-2

    authors: Pal S,Ray A,Andreou C,Zhou Y,Rakshit T,Wlodarczyk M,Maeda M,Toledo-Crow R,Berisha N,Yang J,Hsu HT,Oseledchyk A,Mondal J,Zou S,Kircher MF

    更新日期:2019-04-26 00:00:00

  • Flexible and twistable non-volatile memory cell array with all-organic one diode-one resistor architecture.

    abstract::Flexible organic memory devices are one of the integral components for future flexible organic electronics. However, high-density all-organic memory cell arrays on malleable substrates without cross-talk have not been demonstrated because of difficulties in their fabrication and relatively poor performances to date. H...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms3707

    authors: Ji Y,Zeigler DF,Lee DS,Choi H,Jen AK,Ko HC,Kim TW

    更新日期:2013-01-01 00:00:00

  • Quantum metrology with parametric amplifier-based photon correlation interferometers.

    abstract::Conventional interferometers usually utilize beam splitters for wave splitting and recombination. These interferometers are widely used for precision measurement. Their sensitivity for phase measurement is limited by the shot noise, which can be suppressed with squeezed states of light. Here we study a new type of int...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms4049

    authors: Hudelist F,Kong J,Liu C,Jing J,Ou ZY,Zhang W

    更新日期:2014-01-01 00:00:00

  • Nutrients cause grassland biomass to outpace herbivory.

    abstract::Human activities are transforming grassland biomass via changing climate, elemental nutrients, and herbivory. Theory predicts that food-limited herbivores will consume any additional biomass stimulated by nutrient inputs ('consumer-controlled'). Alternatively, nutrient supply is predicted to increase biomass where her...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-19870-y

    authors: Borer ET,Harpole WS,Adler PB,Arnillas CA,Bugalho MN,Cadotte MW,Caldeira MC,Campana S,Dickman CR,Dickson TL,Donohue I,Eskelinen A,Firn JL,Graff P,Gruner DS,Heckman RW,Koltz AM,Komatsu KJ,Lannes LS,MacDougall AS,Mar

    更新日期:2020-11-27 00:00:00

  • Reconstitution of the complete pathway of ITS2 processing at the pre-ribosome.

    abstract::Removal of internal transcribed spacer 2 (ITS2) from pre-ribosomal RNA is essential to make functional ribosomes. This complicated processing reaction begins with a single endonucleolytic cleavage followed by exonucleolytic trimming at both new cleavage sites to generate mature 5.8S and 25S rRNA. We reconstituted the ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-01786-9

    authors: Fromm L,Falk S,Flemming D,Schuller JM,Thoms M,Conti E,Hurt E

    更新日期:2017-11-27 00:00:00

  • Jagged1-Notch1-deployed tumor perivascular niche promotes breast cancer stem cell phenotype through Zeb1.

    abstract::Zinc finger E-box binding homeobox 1 (Zeb1) has been demonstrated to participate in the acquisition of the properties of cancer stem cells (CSCs). However, it is largely unknown how signals from the tumor microenvironment (TME) contribute to aberrant Zeb1 expression. Here, we show that Zeb1 depletion suppresses stemne...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-18860-4

    authors: Jiang H,Zhou C,Zhang Z,Wang Q,Wei H,Shi W,Li J,Wang Z,Ou Y,Wang W,Wang H,Zhang Q,Sun W,Sun P,Yang S

    更新日期:2020-10-12 00:00:00

  • Transparency and damage tolerance of patternable omniphobic lubricated surfaces based on inverse colloidal monolayers.

    abstract::A transparent coating that repels a wide variety of liquids, prevents staining, is capable of self-repair and is robust towards mechanical damage can have a broad technological impact, from solar cell coatings to self-cleaning optical devices. Here we employ colloidal templating to design transparent, nanoporous surfa...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms3176

    authors: Vogel N,Belisle RA,Hatton B,Wong TS,Aizenberg J

    更新日期:2013-01-01 00:00:00

  • Genetic variation at MECOM, TERT, JAK2 and HBS1L-MYB predisposes to myeloproliferative neoplasms.

    abstract::Clonal proliferation in myeloproliferative neoplasms (MPN) is driven by somatic mutations in JAK2, CALR or MPL, but the contribution of inherited factors is poorly characterized. Using a three-stage genome-wide association study of 3,437 MPN cases and 10,083 controls, we identify two SNPs with genome-wide significance...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms7691

    authors: Tapper W,Jones AV,Kralovics R,Harutyunyan AS,Zoi K,Leung W,Godfrey AL,Guglielmelli P,Callaway A,Ward D,Aranaz P,White HE,Waghorn K,Lin F,Chase A,Baxter EJ,Maclean C,Nangalia J,Chen E,Evans P,Short M,Jack A,Wal

    更新日期:2015-04-07 00:00:00

  • Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.

    abstract::The spindle assembly checkpoint (SAC) arrests cells in mitosis by sensing unattached kinetochores, until all chromosomes are bi-oriented by spindle microtubules. Kinetochore accumulation of the SAC component Mad1-Mad2 is crucial for SAC activation. However, the mechanism by which Mad1-Mad2 accumulation at kinetochores...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms10151

    authors: Zhou H,Wang T,Zheng T,Teng J,Chen J

    更新日期:2016-01-08 00:00:00

  • Coil-globule transitions drive discontinuous volume conserving deformation in locally restrained gels.

    abstract::The equilibrium volume of a thermoresponsive polymer gel changes dramatically across a temperature due to the coil-globule transitions of the polymers. When cofacially oriented nanosheets are embedded in such a gel, the composite gel deforms at the temperature, without changing the volume, and the response time is con...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-04533-w

    authors: Yamamoto T,Masubuchi Y,Doi M

    更新日期:2018-05-25 00:00:00

  • Casimir forces on a silicon micromechanical chip.

    abstract::Quantum fluctuations give rise to van der Waals and Casimir forces that dominate the interaction between electrically neutral objects at sub-micron separations. Under the trend of miniaturization, such quantum electrodynamical effects are expected to play an important role in micro- and nano-mechanical devices. Nevert...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms2842

    authors: Zou J,Marcet Z,Rodriguez AW,Reid MT,McCauley AP,Kravchenko II,Lu T,Bao Y,Johnson SG,Chan HB

    更新日期:2013-01-01 00:00:00

  • Regional boreal biodiversity peaks at intermediate human disturbance.

    abstract::The worldwide biodiversity crisis has intensified the need to better understand how biodiversity and human disturbance are related. The 'intermediate disturbance hypothesis' suggests that disturbance regimes generate predictable non-linear patterns in species richness. Evidence often contradicts intermediate disturban...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms2145

    authors: Mayor SJ,Cahill JF Jr,He F,Sólymos P,Boutin S

    更新日期:2012-01-01 00:00:00

  • Physiological constraints to climate warming in fish follow principles of plastic floors and concrete ceilings.

    abstract::Understanding the resilience of aquatic ectothermic animals to climate warming has been hindered by the absence of experimental systems experiencing warming across relevant timescales (for example, decades). Here, we examine European perch (Perca fluviatilis, L.) from the Biotest enclosure, a unique coastal ecosystem ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms11447

    authors: Sandblom E,Clark TD,Gräns A,Ekström A,Brijs J,Sundström LF,Odelström A,Adill A,Aho T,Jutfelt F

    更新日期:2016-05-17 00:00:00