Abstract:
:Removal of internal transcribed spacer 2 (ITS2) from pre-ribosomal RNA is essential to make functional ribosomes. This complicated processing reaction begins with a single endonucleolytic cleavage followed by exonucleolytic trimming at both new cleavage sites to generate mature 5.8S and 25S rRNA. We reconstituted the 7S→5.8S processing branch within ITS2 using purified exosome and its nuclear cofactors. We find that both Rrp44's ribonuclease activities are required for initial RNA shortening followed by hand over to the exonuclease Rrp6. During the in vitro reaction, ITS2-associated factors dissociate and the underlying 'foot' structure of the pre-60S particle is dismantled. 7S pre-rRNA processing is independent of 5S RNP rotation, but 26S→25S trimming is a precondition for subsequent 7S→5.8S processing. To complete the in vitro assay, we reconstituted the entire cycle of ITS2 removal with a total of 18 purified factors, catalysed by the integrated activities of the two participating RNA-processing machines, the Las1 complex and nuclear exosome.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Fromm L,Falk S,Flemming D,Schuller JM,Thoms M,Conti E,Hurt Edoi
10.1038/s41467-017-01786-9subject
Has Abstractpub_date
2017-11-27 00:00:00pages
1787issue
1issn
2041-1723pii
10.1038/s41467-017-01786-9journal_volume
8pub_type
杂志文章abstract::Pulmonary disease increases the risk of developing abdominal aortic aneurysms (AAA). However, the mechanism underlying the pathological dialogue between the lungs and aorta is undefined. Here, we find that inflicting acute lung injury (ALI) to mice doubles their incidence of AAA and accelerates macrophage-driven prote...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18088-2
更新日期:2020-08-27 00:00:00
abstract::Tomatoes come in a multitude of shapes and flavors despite a narrow genetic pool. Here, we leverage whole-genome resequencing data available for 602 cultivated and wild accessions to determine the contribution of transposable elements (TEs) to tomato diversity. We identify 6,906 TE insertions polymorphisms (TIPs), whi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17874-2
更新日期:2020-08-13 00:00:00
abstract::In nature, protein subunits on the capsids of many icosahedral viruses form rotational patterns, and mathematicians also incorporate asymmetric patterns into faces of polyhedra. Chemists have constructed molecular polyhedra with vacant or highly symmetric faces, but very little is known about constructing polyhedra wi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12469
更新日期:2016-08-24 00:00:00
abstract::A transparent coating that repels a wide variety of liquids, prevents staining, is capable of self-repair and is robust towards mechanical damage can have a broad technological impact, from solar cell coatings to self-cleaning optical devices. Here we employ colloidal templating to design transparent, nanoporous surfa...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3176
更新日期:2013-01-01 00:00:00
abstract::The original version of this Article incorrectly omitted an affiliation of Hongming Weng: "Beijing National Laboratory for Condensed Matter Physics and Institute of Physics, Chinese Academy of Sciences, 100190, Beijing, China"This has been corrected in both the PDF and HTML versions of the Article. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-018-06643-x
更新日期:2018-10-08 00:00:00
abstract::The size of the membrane-bound nucleus scales with cell size in a wide range of cell types but the mechanisms determining overall nuclear size remain largely unknown. Here we investigate the role of fission yeast inner nuclear membrane proteins in determining nuclear size, and propose that the Lap2-Emerin-Man1 domain ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09623-x
更新日期:2019-04-23 00:00:00
abstract::Myelodysplastic syndromes (MDS) are uncommon in children and have a poor prognosis. In contrast to adult MDS, little is known about the genomic landscape of pediatric MDS. Here, we describe the somatic and germline changes of pediatric MDS using whole exome sequencing, targeted amplicon sequencing, and/or RNA-sequenci...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01590-5
更新日期:2017-11-16 00:00:00
abstract::The dynamic incorporation of histone variants influences chromatin structure and many biological processes. In Arabidopsis, the canonical variant H3.1 differs from H3.3 in four residues, one of which (H3.1Phe41) is unique and conserved in plants. However, its evolutionary significance remains unclear. Here, we show th...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-02976-9
更新日期:2018-02-12 00:00:00
abstract::In the bottom-up synthesis of graphene nanoribbons (GNRs) from self-assembled linear polymer intermediates, surface-assisted cyclodehydrogenations usually take place on catalytic metal surfaces. Here we demonstrate the formation of GNRs from quasi-freestanding polymers assisted by hole injections from a scanning tunne...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14815
更新日期:2017-03-13 00:00:00
abstract::Device applications of graphene such as ultrafast transistors and photodetectors benefit from the combination of both high-quality p- and n-doped components prepared in a large-scale manner with spatial control and seamless connection. Here we develop a well-controlled chemical vapour deposition process for direct gro...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2286
更新日期:2012-01-01 00:00:00
abstract::Phospholipase C is a key enzyme in phosphoinositide turnover. Although its functions have been extensively studied at the cellular level, many questions remain concerning its functions at the organ and individual animal levels. Here we demonstrate that mice lacking phospholipase Cδ1 develop granulocytosis associated w...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1960
更新日期:2012-07-17 00:00:00
abstract::Human-machine interfaces have benefited from the advent of wireless sensor networks and the internet of things, but rely on wearable/attachable electronics exhibiting stretchability, biocompatibility, and transmittance. Limited by weight and volume, wearable devices should be energy efficient and even self-powered. He...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03954-x
更新日期:2018-05-04 00:00:00
abstract::Human-induced ocean acidification and warming alter seawater carbonate chemistry reducing the calcification of reef-building crustose coralline algae (CCA), which has implications for reef stability. However, due to the presence of multiple carbonate minerals with different solubilities in seawater, the algal mineralo...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4310
更新日期:2014-01-01 00:00:00
abstract::Centrosome number is tightly controlled during the cell cycle to ensure proper spindle assembly and cell division. However, the underlying mechanism that controls centrosome number remains largely unclear. We show herein that the DNA replication licensing factor Cdc6 is recruited to the proximal side of the centrioles...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15164
更新日期:2017-04-27 00:00:00
abstract::The pyridine ring is a potent pharmacophore in alkaloid natural products. Nonetheless, its biosynthetic pathways are poorly understood. Rubrolones A and B are tropolone alkaloid natural products possessing a unique tetra-substituted pyridine moiety. Here, we report the gene cluster and propose a biosynthetic pathway f...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13083
更新日期:2016-10-07 00:00:00
abstract::We have been alerted that in our recent Article the calculations used to transform the heritability from the observed scale to the liability scale did not take into account the individuals in category 2 of the baldness scale, who were removed in our original analysis. This led to an overestimation of the heritability ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-018-04857-7
更新日期:2018-06-29 00:00:00
abstract::The majority of mammalian genes contain one or more alternative polyadenylation sites. Choice of polyadenylation sites was suggested as one of the underlying mechanisms for generating longer/shorter transcript isoforms. Here, we demonstrate that mature mRNA transcripts can undergo additional cleavage and polyadenylati...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02099-7
更新日期:2017-12-11 00:00:00
abstract::Angiogenesis is a dynamic process relying on endothelial cell rearrangements within vascular tubes, yet the underlying mechanisms and functional relevance are poorly understood. Here we show that PI3Kα regulates endothelial cell rearrangements using a combination of a PI3Kα-selective inhibitor and endothelial-specific...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07172-3
更新日期:2018-11-16 00:00:00
abstract::Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5-13 μs) of photoluminescent porous silicon nanoparticles...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3326
更新日期:2013-01-01 00:00:00
abstract::Autoimmunity and macrophage recruitment into the central nervous system (CNS) are critical determinants of neuroinflammatory diseases. However, the mechanisms that drive immunological responses targeted to the CNS remain largely unknown. Here we show that fibrinogen, a central blood coagulation protein deposited in th...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9164
更新日期:2015-09-10 00:00:00
abstract::BRCA2 is essential for maintaining genomic integrity. BRCA2-deficient primary cells are either not viable or exhibit severe proliferation defects. Yet, BRCA2 deficiency contributes to tumorigenesis. It is believed that mutations in genes such as TRP53 allow BRCA2 heterozygous cells to overcome growth arrest when they ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03020-6
更新日期:2018-02-07 00:00:00
abstract::Semaphorins and their transmembrane receptors, Plexins, are key regulators of axon guidance and development of neuronal connectivity. B-type Plexins respond to Class IV semaphorins and mediate a variety of developmental functions. Here we report that the expression of Plexin-B2 and its high-affinity ligand, Sema4C, pe...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00341-w
更新日期:2017-08-02 00:00:00
abstract::Temporal cloaks have aroused tremendous research interest in both optical physics and optical communications, unfolding a distinct approach to conceal temporal events from an interrogating optical field. The state-of-the-art temporal cloaks exhibit picosecond-scale and static cloaking window, owing to significantly li...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10521-5
更新日期:2019-06-20 00:00:00
abstract::Through the success of deep learning in various domains, artificial neural networks are currently among the most used artificial intelligence methods. Taking inspiration from the network properties of biological neural networks (e.g. sparsity, scale-freeness), we argue that (contrary to general practice) artificial ne...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04316-3
更新日期:2018-06-19 00:00:00
abstract::Culture (behaviour based on socially transmitted information) is present in diverse animal species, yet how it interacts with genetic evolution remains largely unexplored. Here, we review the evidence for gene-culture coevolution in animals, especially birds, cetaceans and primates. We describe how culture can relax o...
journal_title:Nature communications
pub_type: 杂志文章,评审
doi:10.1038/s41467-019-10293-y
更新日期:2019-06-03 00:00:00
abstract::Hydrosilylation of allenes is the addition of a hydrogen atom and a silyl group to a carbon-carbon double bond of an allene molecule and represents a straightforward and atom-economical approach to prepare synthetically versatile allylsilanes and vinylsilanes. However, this reaction generally produces six possible iso...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02382-7
更新日期:2017-12-22 00:00:00
abstract::Novel atherosclerosis models are needed to guide clinical therapy. Here, we report an in vitro model of early atherosclerosis by fabricating and perfusing multi-layer arteriole-scale human tissue-engineered blood vessels (TEBVs) by plastic compression. TEBVs maintain mechanical strength, vasoactivity, and nitric oxide...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19197-8
更新日期:2020-10-27 00:00:00
abstract::Carbon forms one of nature's strongest chemical bonds; its allotropes having provided some of the most exciting scientific discoveries in recent times. The possibility of inter-allotropic transformations/hybridization of carbon is hence a topic of immense fundamental and technological interest. Such modifications usua...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5941
更新日期:2014-09-15 00:00:00
abstract::Functional blood vessel growth depends on generation of distinct but coordinated responses from endothelial cells. Bone morphogenetic proteins (BMP), part of the TGFβ superfamily, bind receptors to induce phosphorylation and nuclear translocation of SMAD transcription factors (R-SMAD1/5/8) and regulate vessel growth. ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13247
更新日期:2016-11-11 00:00:00
abstract::A magnetic impurity coupled to a superconductor gives rise to a Yu-Shiba-Rusinov (YSR) state inside the superconducting energy gap. With increasing exchange coupling the excitation energy of this state eventually crosses zero and the system switches to a YSR ground state with bound quasiparticles screening the impurit...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04683-x
更新日期:2018-06-18 00:00:00