Abstract：
:A specific oligodeoxynucleotide (ODN), ODN MT01, was found to have positive effects on the proliferation and activation of the osteoblast-like cell line MG 63. In this study, the detailed signaling pathways in which ODN MT01 promoted the differentiation of osteoblasts were systematically examined. ODN MT01 enhanced the expression of osteogenic marker genes, such as osteocalcin and type I collagen. Furthermore, ODN MT01 activated Runx2 phosphorylation via ERK1/2 mitogen-activated protein kinase (MAPK) and p38 MAPK. Consistently, ODN MT01 induced up-regulation of osteocalcin, alkaline phosphatase (ALP) and type I collagen, which was inhibited by pre-treatment with the ERK1/2 inhibitor U0126 and the p38 inhibitor SB203580. These results suggest that the ERK1/2 and p38 MAPK pathways, as well as Runx2 activation, are involved in ODN MT01-induced up-regulation of osteocalcin, type I collagen and the activity of ALP in MG 63 cells.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Hou X,Shen Y,Zhang C,Zhang L,Qin Y,Yu Y,Wang L,Sun Xdoi
10.3390/ijms13077902subject
Has Abstractpub_date
2012-01-01 00:00:00pages
7902-14issue
7issn
1422-0067pii
ijms-13-07902journal_volume
13pub_type
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