Abstract:
:Circular dichroism (CD) is a useful technique for monitoring changes in the conformation of antimicrobial peptides or gelatin. In this study, interactions between cationic peptides and gelatin were observed without affecting the triple helical content of the gelatin, which was more strongly affected by anionic surfactant. The peptides did not adopt a secondary structure in the presence of aqueous solution or Tween 80, but a peptide secondary structure formed upon the addition of sodium dodecyl sulfate (SDS). The peptides bound to the phosphate group of lipopolysaccharide (LPS) and displayed an alpha-helical conformation while (KW)(4) adopted a folded conformation. Further, the peptides did not specifically interact with the fungal cell wall components of mannan or laminarin. Tryptophan blue shift assay indicated that these peptides interacted with SDS, LPS, and gelatin but not with Tween 80, mannan, or laminarin. The peptides also displayed antibacterial activity against P. aeruginosa without cytotoxicity against HaCaT cells at MIC, except for HPA3NT3-analog peptide. In this study, we used a CD spectroscopic method to demonstrate the feasibility of peptide characterization in numerous environments. The CD method can thus be used as a screening method of gelatin-peptide interactions for use in wound healing applications.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Gopal R,Park JS,Seo CH,Park Ydoi
10.3390/ijms13033229subject
Has Abstractpub_date
2012-01-01 00:00:00pages
3229-44issue
3issn
1422-0067pii
ijms-13-03229journal_volume
13pub_type
杂志文章abstract::Osteoarthritis (OA) is the most common type of arthritis that occurs in an aged population. It affects any joints in the body and degenerates the articular cartilage and the subchondral bone. Despite the pathophysiology of OA being different, cartilage resorption is still a symbol of osteoarthritis. Matrix metalloprot...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21144931
更新日期:2020-07-13 00:00:00
abstract::The demise of retinal ganglion cells (RGCs) is characteristic of diseases of the retina such as glaucoma and diabetic or ischemic retinopathies. Pigment epithelium-derived factor (PEDF) is a multifunctional secreted protein that mediates neuroprotection and inhibition of angiogenesis in the retina. We have studied exp...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010369
更新日期:2020-12-31 00:00:00
abstract::Esophageal squamous cell carcinoma (ESCC), a major histologic type of esophageal cancer, is one of the frequent causes of cancer-related death worldwide. Picropodophyllotoxin (PPT) is the main component of Podophyllum hexandrum root with antitumor activity via apoptosis-mediated mechanisms in several cancer cells. How...
journal_title:International journal of molecular sciences
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更新日期:2020-06-30 00:00:00
abstract::Procyanidins are a family of plant metabolites that have been suggested to mitigate osteoarthritis pathogenesis in mice. However, the underlying mechanism is largely unknown. This study aimed to determine whether procyanidins mitigate traumatic injury-induced osteoarthritis (OA) disease progression, and whether procya...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17122065
更新日期:2016-12-09 00:00:00
abstract::Targeting cancer cells using chimeric-antigen-receptor (CAR-)T cells has propelled adoptive T-cell therapy (ATT) to the next level. A plentitude of durable complete responses using CD19-specific CAR-T cells in patients suffering from various lymphoid malignancies resulted in the approval by the food and drug administr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
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更新日期:2019-11-26 00:00:00
abstract::After maize seedlings grown in full-strength Hoagland solution for 20 days were exposed to 20% polyethylene glycol (PEG)-stimulated water deficiency for two days, plant height, shoot fresh and dry weights, and pigment contents significantly decreased, whereas malondialdehyde (MDA) content greatly increased. Using tran...
journal_title:International journal of molecular sciences
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更新日期:2015-09-08 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
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更新日期:2020-02-19 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21144914
更新日期:2020-07-12 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21144909
更新日期:2020-07-11 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225633
更新日期:2019-11-11 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141224087
更新日期:2013-12-11 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14058787
更新日期:2013-04-24 00:00:00
abstract::Smart or stimuli-responsive materials are an emerging class of materials used for tissue engineering and drug delivery. A variety of stimuli (including temperature, pH, redox-state, light, and magnet fields) are being investigated for their potential to change a material's properties, interactions, structure, and/or d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21134724
更新日期:2020-07-02 00:00:00
abstract::A dodecadepsipeptide valinomycin (VLM) has been most recently reported to be a potential anti-coronavirus drug that could be efficiently produced on a large scale. It is thus of importance to study solid-phase forms of VLM in order to be able to ensure its polymorphic purity in drug formulations. The previously availa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21144907
更新日期:2020-07-11 00:00:00
abstract::Ischemic preconditioning (IPre) reduces ischemia/reperfusion (I/R) injury in the heart. The non-coding microRNA miR-125b-1-3p has been demonstrated to play a role in the mechanism of IPre. Hypercholesterolemia is known to attenuate the cardioprotective effect of preconditioning; nevertheless, the exact underlying mech...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113744
更新日期:2020-05-26 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165709
更新日期:2020-08-09 00:00:00
abstract::The lignin-carbohydrate complex (LCC) was isolated from milled wood lignin of 2- and 24-month-old crude bamboo (Neosinocalamus affinis) culms using acetic acid (AcOH) and then characterized. The results have shown that the LCC preparation from 2-month-old bamboo (L₂) exhibited a slightly lower molecular weight than th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19010001
更新日期:2017-12-21 00:00:00
abstract::Heme oxygenase (HO) catabolizes heme to produce HO metabolites, such as carbon monoxide (CO) and bilirubin (BR), which have gained recognition as biological signal transduction effectors. The neurovascular unit refers to a highly evolved network among endothelial cells, pericytes, astrocytes, microglia, neurons, and n...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20010078
更新日期:2018-12-25 00:00:00
abstract::Recessively-inherited NR2E3 gene mutations cause an unusual retinopathy with abnormally-increased short-wavelength sensitive cone (S-cone) function, in addition to reduced rod and long/middle-wavelength sensitive cone (L/M-cone) function. Progress toward clinical trials to treat patients with this otherwise incurable ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102497
更新日期:2019-05-21 00:00:00
abstract::To identify whether parent-of-origin effects (POE) of the 15q11.2 BP1-BP2 microdeletion are associated with differences in clinical features in individuals inheriting the deletion, we collected 71 individuals reported with phenotypic data and known inheritance from a clinical cohort, a research cohort, the DECIPHER da...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20061459
更新日期:2019-03-22 00:00:00
abstract::The aqueous solubility (logW) and n-octanol/water partition coefficient (logP(OW)) are important properties for pharmacology, toxicology and medicinal chemistry. Based on an understanding of the dissolution process, the frontier orbital interaction model was suggested in the present paper to describe the solvent-solut...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms9060962
更新日期:2008-06-01 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17071048
更新日期:2016-06-30 00:00:00
abstract::Human cytochrome P450 1B1 (CYP1B1)-mediated biotransformation of endobiotics and xenobiotics plays an important role in the progression of human breast cancer. In this study, we investigated the effects of WY-14643, a peroxisome proliferator-activated receptor α (PPARα) agonist, on CYP1B1 expression and the related me...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20235928
更新日期:2019-11-25 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18091855
更新日期:2017-08-25 00:00:00
abstract::Following major advances in the field of medicinal chemistry, novel drugs can now be designed systematically, instead of relying on old trial and error approaches. Current drug design strategies can be classified as being either ligand- or structure-based depending on the design process. In this paper, by describing t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms12085304
更新日期:2011-01-01 00:00:00
abstract::We have recently found that diabetes-induced premature senescence of retinal endothelial cells is accompanied by NOX2-NADPH oxidase-induced increases in the ureohydrolase enzyme arginase 1 (A1). Here, we used genetic strategies to determine the specific involvement of A1 in diabetes-induced endothelial cell senescence...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19041215
更新日期:2018-04-17 00:00:00
abstract::The frequency and volume of Asian sand dust (ASD) (Kosa) are increasing in Japan, and it has been reported that ASD may cause adverse respiratory effects. The pulmonary toxicity of ASD has been previously analyzed in mice exposed to ASD particles by intratracheal instillation. To study the pulmonary toxicity induced b...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19020416
更新日期:2018-01-31 00:00:00
abstract::Polygalacturonase (PG), a large hydrolase family in plants, is involved in pectin disassembly of the cell wall in plants. The present study aims to characterize PG genes and investigate their expression patterns in Solanum lycopersicum. We identified 54 PG genes in the tomato genome and compared their amino acid seque...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082290
更新日期:2018-08-04 00:00:00
abstract::Drought adversely affects crop production across the globe. The root system immensely contributes to water management and the adaptability of plants to drought stress. In this study, drought-induced phenotypic and transcriptomic responses of two contrasting chickpea (Cicer arietinum L.) genotypes were compared at the ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051781
更新日期:2020-03-05 00:00:00
abstract::Colorectal cancer is one of the leading causes of death, and the third most diagnosed type of cancer, worldwide. It is most common amongst men and women over 50 years old. Risk factors include smoking, alcohol, diet, physical inactivity, genetics, alterations in gut microbiota, and associated pathologies (diabetes, ob...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123787
更新日期:2018-11-28 00:00:00