A coverslip-based technique for evaluating Staphylococcus aureus biofilm formation on human plasma.

Abstract:

:The ability of the opportunistic pathogen, Staphylococcus aureus, to form biofilms is increasingly being viewed as an important contributor to chronic infections. In vitro methods for analyzing S. aureus biofilm formation have focused on bacterial attachment and accumulation on abiotic surfaces, such as in microtiter plate and flow cell assays. Microtiter plates provide a rapid measure of relative biomass levels, while flow cells have limited experimental throughput but are superior for confocal microscopy biofilm visualization. Although these assays have proven effective at identifying mechanisms involved in cell attachment and biofilm accumulation, the significance of these assays in vivo remains unclear. Studies have shown that when medical devices are implanted they are coated with host factors, such as matrix proteins, that facilitate S. aureus attachment and biofilm formation. To address the challenge of integrating existing biofilm assay features with a biotic surface, we have established an in vitro biofilm technique utilizing UV-sterilized coverslips coated with human plasma. The substratum more closely resembles the in vivo state and provides a platform for S. aureus to establish a robust biofilm. Importantly, these coverslips are amenable to confocal microscopy imaging to provide a visual reference of the biofilm growth stage, effectively merging the benefits of the microtiter and flow cell assays. We confirmed the approach using clinical S. aureus isolates and mutants with known biofilm phenotypes. Altogether, this new biofilm assay can be used to assess the function of S. aureus virulence factors associated with biofilm formation and for monitoring the efficacy of biofilm treatment modalities.

authors

Walker JN,Horswill AR

doi

10.3389/fcimb.2012.00039

subject

Has Abstract

pub_date

2012-03-27 00:00:00

pages

39

issn

2235-2988

journal_volume

2

pub_type

杂志文章
  • Extreme Diversity of Mycoviruses Present in Isolates of Rhizoctonia solani AG2-2 LP From Zoysia japonica From Brazil.

    abstract::Zoysia japonica, in Brazil, is commonly infected by Rhizoctonia solani (R. solani) in humid and cool weather conditions. Eight isolates of R. solani, previously identified as belonging to the AG2-2 LP anastomosis group, isolated from samples from large path symptoms, were collected from three counties in São Paulo sta...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00244

    authors: Picarelli MASC,Forgia M,Rivas EB,Nerva L,Chiapello M,Turina M,Colariccio A

    更新日期:2019-07-12 00:00:00

  • Serotyping of Toxoplasma gondii Infection Using Peptide Membrane Arrays.

    abstract::The intracellular parasite Toxoplasma gondii can cause chronic infections in most warm-blooded animals, including humans. In the USA, strains belonging to four different Toxoplasma clonal lineages (types 1, 2, 3, and 12) are commonly isolated, whereas strains not belonging to these lineages are predominant in other co...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00408

    authors: Arranz-Solís D,Cordeiro C,Young LH,Dardé ML,Commodaro AG,Grigg ME,Saeij JPJ

    更新日期:2019-11-29 00:00:00

  • Caspofungin Induced Cell Wall Changes of Candida Species Influences Macrophage Interactions.

    abstract::Candida species are known to differ in their ability to cause infection and have been shown to display varied susceptibilities to antifungal drugs. Treatment with the echinocandin, caspofungin, leads to compensatory alterations in the fungal cell wall. This study was performed to compare the structure and composition ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00164

    authors: Walker LA,Munro CA

    更新日期:2020-05-12 00:00:00

  • Production of Membrane Vesicles by Enterococcus faecium Cultured With or Without Subinhibitory Concentrations of Antibiotics and Their Pathological Effects on Epithelial Cells.

    abstract::Enterococcus faecium is a clinically important pathogen associated with opportunistic infection and multi-drug resistance. E. faecium has been shown to produce membrane vesicles (MVs), but MV production by E. faecium under antibiotic stress conditions and the pathogenic traits thereof have yet to be determined. This s...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00295

    authors: Kim MH,Kim SY,Son JH,Kim SI,Lee H,Kim S,Shin M,Lee JC

    更新日期:2019-08-14 00:00:00

  • Internalization of the Active Subunit of the Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Is Dependent upon Cellugyrin (Synaptogyrin 2), a Host Cell Non-Neuronal Paralog of the Synaptic Vesicle Protein, Synaptogyrin 1.

    abstract::The Aggregatibacter actinomycetemcomitans cytolethal distending toxin (Cdt) is a heterotrimeric AB2 toxin capable of inducing lymphocytes, and other cell types, to undergo cell cycle arrest and apoptosis. Exposure to Cdt results in binding to the cell surface followed by internalization and translocation of the active...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00469

    authors: Boesze-Battaglia K,Walker LP,Dhingra A,Kandror K,Tang HY,Shenker BJ

    更新日期:2017-11-14 00:00:00

  • Links between type III secretion and extracytoplasmic stress responses in Yersinia.

    abstract::The cell envelope of pathogenic bacteria is a barrier against host environmental conditions and immunity molecules, as well as the site where many virulence factors are assembled. Extracytoplasmic stress responses (ESRs) have evolved to help maintain its integrity in conditions where it might be compromised. These ESR...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2012.00125

    authors: Flores-Kim J,Darwin AJ

    更新日期:2012-10-10 00:00:00

  • Mapping Arbovirus-Vector Interactions Using Systems Biology Techniques.

    abstract::Studying how arthropod-borne viruses interact with their arthropod vectors is critical to understanding how these viruses replicate and are transmitted. Until recently, these types of studies were limited in scale because of the lack of classical tools available to study virus-host interaction for non-model viruses an...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2018.00440

    authors: Petit MJ,Shah PS

    更新日期:2019-01-07 00:00:00

  • SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes.

    abstract::Streptococcus pyogenes (Group A Streptococcus or GAS) is a hemolytic human pathogen associated with a wide variety of infections ranging from minor skin and throat infections to life-threatening invasive diseases. The cell wall of GAS consists of peptidoglycan sacculus decorated with a carbohydrate comprising a polyrh...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2016.00126

    authors: Edgar RJ,Chen J,Kant S,Rechkina E,Rush JS,Forsberg LS,Jaehrig B,Azadi P,Tchesnokova V,Sokurenko EV,Zhu H,Korotkov KV,Pancholi V,Korotkova N

    更新日期:2016-10-13 00:00:00

  • Challenges in the culture-independent analysis of oral and respiratory samples from intubated patients.

    abstract::The spread of microorganisms in hospitals is an important public health threat, and yet few studies have assessed how human microbial communities (microbiota) evolve in the hospital setting. Studies conducted so far have mainly focused on a limited number of bacterial species, mostly pathogenic ones and primarily duri...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2014.00065

    authors: Lazarevic V,Gaïa N,Emonet S,Girard M,Renzi G,Despres L,Wozniak H,Yugueros Marcos J,Veyrieras JB,Chatellier S,van Belkum A,Pugin J,Schrenzel J

    更新日期:2014-05-23 00:00:00

  • Genome-Wide Transcriptional Start Site Mapping and sRNA Identification in the Pathogen Leptospira interrogans.

    abstract::Leptospira are emerging zoonotic pathogens transmitted from animals to humans typically through contaminated environmental sources of water and soil. Regulatory pathways of pathogenic Leptospira spp. underlying the adaptive response to different hosts and environmental conditions remains elusive. In this study, we pro...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00010

    authors: Zhukova A,Fernandes LG,Hugon P,Pappas CJ,Sismeiro O,Coppée JY,Becavin C,Malabat C,Eshghi A,Zhang JJ,Yang FX,Picardeau M

    更新日期:2017-01-19 00:00:00

  • Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in São Paulo State, Brazil.

    abstract::Ocular toxoplasmosis is one of the most common complications caused by the infection with the parasite Toxoplasma gondii. The risk of developing eye lesions and impaired vision is considered higher in Brazil than other countries. The clinical diagnosis is difficult and the use of sensitive and specific laboratorial me...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00472

    authors: Murata FHA,Previato M,Frederico FB,Barbosa AP,Nakashima F,de Faria GM Jr,Silveira Carvalho AP,Meira Strejevitch CDS,Pereira-Chioccola VL,Castiglioni L,de Mattos LC,Siqueira RC,Brandão de Mattos CC

    更新日期:2020-02-07 00:00:00

  • CRH Affects the Phenotypic Expression of Sepsis-Associated Virulence Factors by Streptococcus pneumoniae Serotype 1 In vitro.

    abstract::Sepsis is a life-threatening health condition caused by infectious pathogens of the respiratory tract, and accounts for 28-50% of annual deaths in the US alone. Current treatment regimen advocates the use of corticosteroids as adjunct treatment with antibiotics, for their broad inhibitory effect on the activity and pr...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00263

    authors: Ngo Ndjom CG,Kantor LV,Jones HP

    更新日期:2017-06-22 00:00:00

  • Commensal E. coli Stx2 lysogens produce high levels of phages after spontaneous prophage induction.

    abstract::Enterohemorrhagic E. coli (EHEC) is a food-borne pathogen that causes disease ranging from uncomplicated diarrhea to life-threatening hemolytic uremic syndrome (HUS) and nervous system complications. Shiga toxin 2 (Stx2) is the major virulence factor of EHEC and is critical for development of HUS. The genes encoding S...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2015.00005

    authors: Iversen H,L' Abée-Lund TM,Aspholm M,Arnesen LP,Lindbäck T

    更新日期:2015-02-03 00:00:00

  • Genetic Diversity and Phylogeography of Thottapalayam thottimvirus (Hantaviridae) in Asian House Shrew (Suncus murinus) in Eurasia.

    abstract::Murid and cricetid rodents were previously believed to be the principal reservoir hosts of hantaviruses. Recently, however, multiple newfound hantaviruses have been discovered in shrews, moles, and bats, suggesting a complex evolutionary history. Little is known about the genetic diversity and geographic distribution ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00438

    authors: Kikuchi F,Aoki K,Ohdachi SD,Tsuchiya K,Motokawa M,Jogahara T,Sơn NT,Bawm S,Lin KS,Thwe TL,Gamage CD,Ranorosoa MC,Omar H,Maryanto I,Suzuki H,Tanaka-Taya K,Morikawa S,Mizutani T,Suzuki M,Yanagihara R,Arai S

    更新日期:2020-08-27 00:00:00

  • Genetic regulation of the intercellular adhesion locus in staphylococci.

    abstract::The formation of biofilms by Staphylococcus aureus and Staphylococcus epidermidis is an important aspect of many staphylococcal infections, most notably endocarditis, osteomyelitis and infections associated with indwelling medical devices. The major constituents of staphylococcal biofilms are polysaccharides, such as ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2012.00038

    authors: Cue D,Lei MG,Lee CY

    更新日期:2012-03-26 00:00:00

  • Genetic and Virulence Profiles of Enteroaggregative Escherichia coli (EAEC) Isolated From Deployed Military Personnel (DMP) With Travelers' Diarrhea.

    abstract::To discern if there was a particular genotype associated with clinical enteroaggregative Escherichia coli (EAEC) strains isolated from deployed military personnel (DMP) with travelers' diarrhea (TD), we characterized a collection of EAEC from DMP deployed to Afghanistan, Djibouti, Kenya, or Honduras. Although we did n...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00200

    authors: Petro CD,Duncan JK,Seldina YI,Allué-Guardia A,Eppinger M,Riddle MS,Tribble DR,Johnson RC,Dalgard CL,Sukumar G,Connor P,Boisen N,Melton-Celsa AR

    更新日期:2020-05-20 00:00:00

  • Comparison of Subgingival and Buccal Mucosa Microbiome in Chronic and Aggressive Periodontitis: A Pilot Study.

    abstract::Periodontal microorganisms not only colonize subgingival pockets, but also are detected on various mucous membranes in patients with periodontitis. The object of this pilot study was, using the next-generation sequencing of 16S RNA gene, to characterize the microbiota in two oral habitats (buccal mucosas and subgingiv...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00053

    authors: Wei Y,Shi M,Zhen M,Wang C,Hu W,Nie Y,Wu X

    更新日期:2019-03-11 00:00:00

  • Live probiotic cultures and the gastrointestinal tract: symbiotic preservation of tolerance whilst attenuating pathogenicity.

    abstract::Bacteria comprise the earliest form of independent life on this planet. Bacterial development has included co-operative symbiosis with plants (e.g., Leguminosae family and nitrogen fixing bacteria in soil) and animals (e.g., the gut microbiome). A fusion event of two prokaryotes evolutionarily gave rise to the eukaryo...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2014.00143

    authors: Vitetta L,Hall S,Linnane AW

    更新日期:2014-10-15 00:00:00

  • The Influence of Proton Pump Inhibitors on the Fecal Microbiome of Infants with Gastroesophageal Reflux-A Prospective Longitudinal Interventional Study.

    abstract::Proton pump inhibitors (PPIs) are the standard therapy for gastroesophageal reflux disease. In adults, PPI treatment is associated with Clostridium difficile infections (CDI). In contrast to adults the microbiome of infants develops from sterility at birth toward an adult-like profile in the first years of life. The e...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 临床试验,杂志文章

    doi:10.3389/fcimb.2017.00444

    authors: Castellani C,Singer G,Kashofer K,Huber-Zeyringer A,Flucher C,Kaiser M,Till H

    更新日期:2017-10-11 00:00:00

  • The NAD+ Responsive Transcription Factor ERM-BP Functions Downstream of Cellular Aggregation and Is an Early Regulator of Development and Heat Shock Response in Entamoeba.

    abstract::Entamoeba histolytica is a protozoan parasite and a major cause of dysentery and diarrheal disease in developing countries. Disease transmission from one host to another occurs via cysts which can survive in environmental extremes and are transmitted through contaminated food and water. Recent studies in our lab ident...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00363

    authors: Manna D,Lozano-Amado D,Ehrenkaufer G,Singh U

    更新日期:2020-07-17 00:00:00

  • Cytolethal Distending Toxin Enhances Radiosensitivity in Prostate Cancer Cells by Regulating Autophagy.

    abstract::Cytolethal distending toxin (CDT) produced by Campylobacter jejuni contains three subunits: CdtA, CdtB, and CdtC. Among these three toxin subunits, CdtB is the toxic moiety of CDT with DNase I activity, resulting in DNA double-strand breaks (DSB) and, consequently, cell cycle arrest at the G2/M stage and apoptosis. Ra...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00223

    authors: Lin HJ,Liu HH,Lin CD,Kao MC,Chen YA,Chiang-Ni C,Jiang ZP,Huang MZ,Lin CJ,Lo UG,Lin LC,Lai CK,Lin H,Hsieh JT,Chiu CH,Lai CH

    更新日期:2017-06-08 00:00:00

  • Peptide Occurring in Enterobacteriaceae Triggers Streptococcus pneumoniae Cell Death.

    abstract::Non-encapsulated Streptococcus pneumoniae often possess two genes, aliB-like ORF 1 and aliB-like ORF 2, in place of capsule genes. AliB-like ORF 1 is thought to encode a substrate binding protein of an ABC transporter which binds peptide SETTFGRDFN, found in 50S ribosomal subunit protein L4 of Enterobacteriaceae. Here...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2019.00320

    authors: Nasher F,Kwun MJ,Croucher NJ,Heller M,Hathaway LJ

    更新日期:2019-09-10 00:00:00

  • High-Throughput Microplate-Based Assay to Monitor Plasma Membrane Wounding and Repair.

    abstract::The plasma membrane of mammalian cells is susceptible to disruption by mechanical and biochemical damages that frequently occur within tissues. Therefore, efficient and rapid repair of the plasma membrane is essential for maintaining cellular homeostasis and survival. Excessive damage of the plasma membrane and defect...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00305

    authors: Pathak-Sharma S,Zhang X,Lam JGT,Weisleder N,Seveau SM

    更新日期:2017-07-14 00:00:00

  • Generation of an Attenuated Tiantan Vaccinia Virus Strain by Deletion of Multiple Genes.

    abstract::An attenuated vaccinia virus-MVTTEAB-was constructed by deletion of non-essential gene segments related to the immunomodulatory and virulence functions of the vaccinia virus Tiantan strain (VVTT). The shuttle plasmids pTC-EGFP, pTE-EGFP, pTA35-EGFP, pTB-EGFP, and pTA66-EGFP were constructed and combined with the early...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2017.00462

    authors: Li Y,Zhu Y,Chen S,Li W,Yin X,Li S,Xiao P,Han J,Li X,Sun L,Jin N

    更新日期:2017-10-31 00:00:00

  • MicroRNA-199a Inhibits Cellular Autophagy and Downregulates IFN-β Expression by Targeting TBK1 in Mycobacterium bovis Infected Cells.

    abstract::The mechanism by which microRNAs (miRNAs) modulate innate immunity and autophagy has not been fully elucidated in Mycobacterium bovis (M. bovis) infections. In this study, we identified that miR-199a inhibited key innate immune responses and autophagy in murine macrophages infected with M. bovis. Using ex vivo and in ...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2018.00238

    authors: Wang J,Hussain T,Yue R,Liao Y,Li Q,Yao J,Song Y,Sun X,Wang N,Xu L,Sreevatsan S,Zhao D,Zhou X

    更新日期:2018-07-10 00:00:00

  • BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions.

    abstract::Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocat...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2013.00028

    authors: Salcedo SP,Marchesini MI,Degos C,Terwagne M,Von Bargen K,Lepidi H,Herrmann CK,Santos Lacerda TL,Imbert PR,Pierre P,Alexopoulou L,Letesson JJ,Comerci DJ,Gorvel JP

    更新日期:2013-07-08 00:00:00

  • The Complement Binding and Inhibitory Protein CbiA of Borrelia miyamotoi Degrades Extracellular Matrix Components by Interacting with Plasmin(ogen).

    abstract::The emerging relapsing fever spirochete Borrelia (B.) miyamotoi is transmitted by ixodid ticks and causes the so-called hard tick-borne relapsing fever or B. miyamotoi disease (BMD). More recently, we identified a surface-exposed molecule, CbiA exhibiting complement binding and inhibitory capacity and rendering spiroc...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2018.00023

    authors: Nguyen NTT,Röttgerding F,Devraj G,Lin YP,Koenigs A,Kraiczy P

    更新日期:2018-02-02 00:00:00

  • Rapid Detection of Nocardia by Next-Generation Sequencing.

    abstract::In this original study, we retrospectively reviewed the cases of nocardiosis diagnosed through culture and next-generation sequencing (NGS) methods between 2014 and 2018 in Huashan Hospital and found out that the latter way can not only improve the detection rate of Nocardia spp. but also greatly reduce the turnaround...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00013

    authors: Weng SS,Zhang HY,Ai JW,Gao Y,Liu YY,Xu B,Zhang WH

    更新日期:2020-02-18 00:00:00

  • Autophagy and Ubiquitination in Salmonella Infection and the Related Inflammatory Responses.

    abstract::Salmonellae are facultative intracellular pathogens that cause globally distributed diseases with massive morbidity and mortality in humans and animals. In the past decades, numerous studies were focused on host defenses against Salmonella infection. Autophagy has been demonstrated to be an important defense mechanism...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fcimb.2018.00078

    authors: Wang L,Yan J,Niu H,Huang R,Wu S

    更新日期:2018-03-14 00:00:00

  • Francisella novicida Two-Component System Response Regulator BfpR Modulates iglC Gene Expression, Antimicrobial Peptide Resistance, and Biofilm Production.

    abstract::Response regulators are a critical part of the two-component system of gene expression regulation in bacteria, transferring a signal from a sensor kinase into DNA binding activity resulting in alteration of gene expression. In this study, we investigated a previously uncharacterized response regulator in Francisella n...

    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章

    doi:10.3389/fcimb.2020.00082

    authors: Dean SN,Milton ME,Cavanagh J,van Hoek ML

    更新日期:2020-03-13 00:00:00