Efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons.

abstract：:Sialyl Lewis x (sLe(x)) and sialyl Lewis a (sLe(a)) glycans are expressed on highly metastatic colon cancer cells. They promote extravasation of cancer cells and tumor angiogenesis via interacting with E-selectin on endothelial cells. Recently, epithelial-mesenchymal transition (EMT) has been noted as a critical pheno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakuma K,Aoki M,Kannagi R

更新日期：2012-05-15 00:00:00

abstract：:Normal rat mast cells were stimulated by antibodies against IgE receptors (anti-RBL) or by anti-IgE, and [3H]methyl group incorporation into phospholipids, 45Ca uptake, and histamine release were examined. Anti-RBL or its F(ab')2 fragments and anti-IgE induced an increase in the incorporation of [3H]methyl into phosph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishizaka T,Hirata F,Ishizaka K,Axelrod J

更新日期：1980-04-01 00:00:00

abstract：:Social media sites are rapidly becoming one of the most important forums for public deliberation about advocacy issues. However, social scientists have not explained why some advocacy organizations produce social media messages that inspire far-ranging conversation among social media users, whereas the vast majority o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bail CA

更新日期：2016-10-18 00:00:00

abstract：:The delta-subspecies of protein kinase C (delta PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose fast flow, phenyl 5PW, heparin 5PW, hydroxyapatite, and Mono Q columns. The purified enzyme was a doublet with molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ogita K,Miyamoto S,Yamaguchi K,Koide H,Fujisawa N,Kikkawa U,Sahara S,Fukami Y,Nishizuka Y

更新日期：1992-03-01 00:00:00

abstract：:Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheong CG,Hall TM

更新日期：2006-09-12 00:00:00

abstract：:Filamentous actin (F-actin) cytoskeletal remodeling is critical for glucose-stimulated insulin secretion (GSIS) in pancreatic β-cells, and its dysregulation causes type 2 diabetes. The adaptor protein APPL1 promotes first-phase GSIS by up-regulating soluble N-ethylmaleimide-sensitive factor attachment protein receptor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang B,Lin H,Li X,Lu W,Kim JB,Xu A,Cheng KKY

更新日期：2020-11-10 00:00:00

abstract：:Progesterone is involved in the development and progression of breast cancers, and progesterone receptors (PR) are important markers of hormone dependence and disease prognosis. We have used a human PR cDNA probe, genomic DNA blotting of a series of Chinese hamster-human cell hybrids, and in situ hybridization to map ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Law ML,Kao FT,Wei Q,Hartz JA,Greene GL,Zarucki-Schulz T,Conneely OM,Jones C,Puck TT,O'Malley BW

更新日期：1987-05-01 00:00:00

abstract：:Genetic switches based on the [Formula: see text] system are master regulators of an array of cellular responses. Recent kinetic experiments have shown that [Formula: see text] can actively remove NF-κB bound to its genetic sites via a process called "molecular stripping." This allows the [Formula: see text] switch to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Potoyan DA,Zheng W,Komives EA,Wolynes PG

更新日期：2016-01-05 00:00:00

abstract：:Tissues from rhesus monkeys were screened by PCR for the presence of sequences homologous to known adeno-associated virus (AAV) serotypes 1-6. DNA spanning entire rep-cap ORFs from two novel AAVs, called AAV7 and AAV8, were isolated. Sequence comparisons among these and previously described AAVs revealed the greatest ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao GP,Alvira MR,Wang L,Calcedo R,Johnston J,Wilson JM

更新日期：2002-09-03 00:00:00

abstract：:The parathyroid hormone 2 (PTH2) receptor's anatomical distribution suggests that, among other functions, it may be involved in modulation of nociception. We localized PTH2 receptor protein to spinal cord lamina II and showed that it is synthesized by subpopulations of primary sensory neurons and intrinsic spinal cord...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dobolyi A,Ueda H,Uchida H,Palkovits M,Usdin TB

更新日期：2002-02-05 00:00:00

abstract：:When a local mechanical perturbation is applied to the surface of a thin film of a mechanically interlocked molecule (a rotaxane), the molecules self-organize into periodic arrays of discrete dots or lines. The dimensionality of the nanostructures depends on whether the mechanical stimulus acts along a 1D line or over...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biscarini F,Cavallini M,Kshirsagar R,Bottari G,Leigh DA,León S,Zerbetto F

更新日期：2006-11-21 00:00:00

abstract：:Unit-resolution mass spectra have been obtained for peptides as large as 17 kDa, providing information on impurities and adduct ions, as well as accurate molecular weight values. Electrospray ionization produces many multiply-charged species of the same mass; isotopic peak resolution provides direct charge state assig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loo JA,Quinn JP,Ryu SI,Henry KD,Senko MW,McLafferty FW

更新日期：1992-01-01 00:00:00

abstract：:To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauer S,Vasu P,Persson S,Mort AJ,Somerville CR

更新日期：2006-07-25 00:00:00

abstract：:Netrin-1 is critical for axonal pathfinding which shares similarities with formation of vascular network. Here we report that netrin-1 induction of angiogenesis is mediated by an increase in endothelial nitric oxide (NO*) production, which occurs via a DCC-dependent, ERK1/2-eNOS feed-forward mechanism. Exposure of mat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen A,Cai H

更新日期：2006-04-25 00:00:00

abstract：:A detailed experimental investigation of Fe1+y Te (y = 0.11, 0.12) using pulsed magnetic fields up to 60 T confirms remarkable magnetic shape-memory (MSM) effects. These effects result from magnetoelastic transformation processes in the low-temperature antiferromagnetic state of these materials. The observation of mod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rößler S,Koz C,Wang Z,Skourski Y,Doerr M,Kasinathan D,Rosner H,Schmidt M,Schwarz U,Rößler UK,Wirth S

更新日期：2019-08-20 00:00:00

abstract：:Musical training is known to modify cortical organization. Here, we show that such modifications extend to subcortical sensory structures and generalize to processing of speech. Musicians had earlier and larger brainstem responses than nonmusician controls to both speech and music stimuli presented in auditory and aud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musacchia G,Sams M,Skoe E,Kraus N

更新日期：2007-10-02 00:00:00

abstract：:The eukaryotic small ribosomal subunit carries only four ribosomal (r) RNA methylated bases, all close to important functional sites. N(7)-methylguanosine (m(7)G) introduced at position 1575 on 18S rRNA by Bud23-Trm112 is at a ridge forming a steric block between P- and E-site tRNAs. Here we report atomic resolution s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Létoquart J,Huvelle E,Wacheul L,Bourgeois G,Zorbas C,Graille M,Heurgué-Hamard V,Lafontaine DL

更新日期：2014-12-23 00:00:00

abstract：:Naive CD4 T cells differentiate into several effector lineages, which generate a stronger and more rapid response to previously encountered immunological challenges. Although effector function is a key feature of adaptive immunity, the molecular basis of this process is poorly understood. Here, we investigated the spa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zorca CE,Kim LK,Kim YJ,Krause MR,Zenklusen D,Spilianakis CG,Flavell RA

更新日期：2015-03-31 00:00:00

abstract：:MicroRNAs are predicted to regulate approximately 30% of all human genes by targeting sequences in their 3' UTR. Polymorphisms in 3' UTR of several genes have been reported to affect gene expression, but the mechanism is not fully understood. Here, we demonstrate that 829C-->T, a naturally occurring SNP, near the miR-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mishra PJ,Humeniuk R,Mishra PJ,Longo-Sorbello GS,Banerjee D,Bertino JR

更新日期：2007-08-14 00:00:00

abstract：:Duplexes constituted by closed or open RNA circles paired to single-stranded oligonucleotides terminating with 3'-CCAOH form resected pseudoknots that are substrates of yeast histidyl-tRNA synthetase. Design of this RNA fold is linked to the mimicry of the pseudoknotted amino acid accepting branch of the tRNA-like dom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felden B,Giegé R

更新日期：1998-09-01 00:00:00

abstract：:A striking feature of the human genome is the dearth of CpG dinucleotides (CpGs) interrupted occasionally by CpG islands (CGIs), regions with relatively high content of the dinucleotide. CGIs are generally associated with promoters; genes, whose promoters are especially rich in CpG sequences, tend to be expressed in m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saxonov S,Berg P,Brutlag DL

更新日期：2006-01-31 00:00:00

abstract：:E2A helix-loop-helix proteins are involved in the control of various developmental pathways. We show here by in situ hybridization that E2A transcripts are present in most embryonic and adult tissues. However, no E2A expression is detectable in heart and nonproliferative regions of the brain and spinal cord. Highest l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts VJ,Steenbergen R,Murre C

更新日期：1993-08-15 00:00:00

abstract：:The neuropeptide galanin is expressed developmentally in the dorsal root ganglion (DRG) and is rapidly up-regulated 120-fold after peripheral nerve section in the adult. Here we report that adult mice carrying a loss-of-function mutation in the galanin gene have a 13% reduction in the number of cells in the DRG associ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes FE,Mahoney S,King VR,Bacon A,Kerr NC,Pachnis V,Curtis R,Priestley JV,Wynick D

更新日期：2000-10-10 00:00:00

abstract：:Many aspects of the evolutionary process of tumorigenesis that are fundamental to cancer biology and targeted treatment have been challenging to reveal, such as the divergence times and genetic clonality of metastatic lineages. To address these challenges, we performed tumor phylogenetics using molecular evolutionary ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao ZM,Zhao B,Bai Y,Iamarino A,Gaffney SG,Schlessinger J,Lifton RP,Rimm DL,Townsend JP

更新日期：2016-02-23 00:00:00

abstract：:Acid-sensing ion channels (ASICs) are neuronal non-voltage-gated cation channels that are activated when extracellular pH falls. They contribute to sensory function and nociception in the peripheral nervous system, and in the brain they contribute to synaptic plasticity and fear responses. Some of the physiologic cons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petroff EY,Price MP,Snitsarev V,Gong H,Korovkina V,Abboud FM,Welsh MJ

更新日期：2008-02-26 00:00:00

abstract：:Oxidation of molecular hydrogen catalyzed by [NiFe] hydrogenases is a widespread mechanism of energy generation among prokaryotes. Biosynthesis of the H2-oxidizing enzymes is a complex process subject to positive control by H2 and negative control by organic energy sources. In this report we describe a novel signal tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lenz O,Friedrich B

更新日期：1998-10-13 00:00:00

abstract：:Several lines of investigation suggest that the nondegradable fluorophores that accumulate as lipofuscin in retinal pigment epithelium (RPE) cells contribute to the etiology of macular degeneration. Despite evidence that much of this fluorescent material may originate as inadvertent products of the retinoid cycle, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fishkin NE,Sparrow JR,Allikmets R,Nakanishi K

更新日期：2005-05-17 00:00:00

abstract：:The expression of a number of genes encoding products involved in copper ion uptake in yeast is specifically inhibited by copper ions. We show here that copper metalloregulation occurs through Cu-dependent repression of the transactivation activity of Mac1p. A segment of the yeast transcription factor Mac1p was identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graden JA,Winge DR

更新日期：1997-05-27 00:00:00

abstract：:The structurally diverse peroxisome proliferators ciprofibrate, clofibrate, and bis(2-ethylhexyl) phthalate [(EtHx)2 greater than Pht] increase the activities of hepatic catalase and peroxisomal fatty acid beta-oxidation enzymes in conjunction with profound proliferation of peroxisomes in hepatocytes. In order to deli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reddy JK,Goel SK,Nemali MR,Carrino JJ,Laffler TG,Reddy MK,Sperbeck SJ,Osumi T,Hashimoto T,Lalwani ND

更新日期：1986-03-01 00:00:00

abstract：:Heparin is the most widely prescribed biopharmaceutical in production globally. Its potent anticoagulant activity and safety makes it the drug of choice for preventing deep vein thrombosis and pulmonary embolism. In 2008, adulterated material was introduced into the heparin supply chain, resulting in several hundred d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss RJ,Spahn PN,Toledo AG,Chiang AWT,Kellman BP,Li J,Benner C,Glass CK,Gordts PLSM,Lewis NE,Esko JD

更新日期：2020-04-28 00:00:00