当前位置： SCI文献检索 > EUROPEAN JOURNAL OF PHARMACOLOGY期刊下所有文献 > Resveratrol augments nitric oxide generation and causes store calcium release in chromaffin cells.

Resveratrol augments nitric oxide generation and causes store calcium release in chromaffin cells.

Abstract：

:The cardiovascular protecting effect of the grape fruit trans-resveratrol has been explained among other factors, through augmentation of nitric oxide (NO) production in cardiovascular tissues. Another effect of low resveratrol concentration is the inhibition of single-vesicle quantal release of catecholamine from bovine adrenal chromaffin cells, that was recently suggested to be an additional factor contributing to its beneficial cardiovascular effects. We have investigated here the effects of a low concentration of trans-resveratrol (1 μM) on Ca(2+) and NO signaling pathways in bovine chromaffin cells, in an attempt to understand the mechanism underlying its previously reported inhibitory effects on quantal secretion. In cells loaded with fura-2 acetoxymethyl ester (fura-2), we have found that 1 μM resveratrol produces a transient elevation of the cytosolic Ca(2+) concentration ([Ca(2+)](c)). This Ca(2+) transient was drastically reduced when the Ca(2+) store was depleted by ryanodine and dantrolene; it was also inhibited by N(ω)-nitro-l-arginine methyl ester hydrochloride (L-NAME) and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). Furthermore, the Ca(2+) transient was mimicked by NO donor S-nitroso-N-acetyl-penicillamine (SNAP). Resveratrol also enhanced the production of nitrites and NO, and L-NAME blocked both responses; in contrast, augmentation by SNAP of nitrites and NO was unaffected by ODQ and was only partially inhibited by L-NAME. On the basis of these results, we are proposing that resveratrol is mitigating the catecholamine surge occurring during stress, through its ability to elicit mild local [Ca(2+)](c) transients and enhanced NO production, that blocks the last steps of exocytosis.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Padín JF,de Diego AM,Fernández-Morales JC,Merino C,Maroto M,Calvo-Gallardo E,Arranz JA,Yáñez M,García AG

doi

10.1016/j.ejphar.2012.03.040

subject

Has Abstract

pub_date

2012-06-15 00:00:00

pages

99-107

issue

1-3

eissn

0014-2999

issn

1879-0712

pii

S0014-2999(12)00300-7

journal_volume

685

pub_type

杂志文章

在线工具

Resveratrol augments nitric oxide generation and causes store calcium release in chromaffin cells.