Abstract：
:Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure precisely the unfolding relaxation time as well as the helical fraction evolution. We show that only CD at 220 nm is relevant to observe the unfolding of an alpha helix whereas no change is observed for CD at 204 nm. Conversely, both absorptions yield information on the dynamics of the process.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Mendonça L,Hache Fdoi
10.3390/ijms13022239subject
Has Abstractpub_date
2012-01-01 00:00:00pages
2239-48issue
2issn
1422-0067pii
ijms-13-02239journal_volume
13pub_type
杂志文章abstract：:Ca2+ signaling has been involved in controling critical cellular functions such as activation of proteases, cell death, and cell cycle control. The endoplasmatic reticulum plays a significant role in Ca2+ storage inside the cell, but mitochondria have long been recognized as a fundamental Ca2+ pool. Protozoan parasite...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms22010469
更新日期：2021-01-05 00:00:00
abstract：:Omega-3 fatty acids are important to pregnancy and neonatal development and health. One mechanism by which omega-3 fatty acids exert their protective effects is through serving as substrates for the generation of specialized pro-resolving lipid mediators (SPM) that potently limit and resolve inflammatory processes. We...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184402
更新日期：2019-09-07 00:00:00
abstract：:To combat the escalating rise of antibacterial resistance, the development of antimicrobial peptides (AMPs) with a unique mode of action is considered an attractive strategy. However, proteolytic degradation of AMPs remains the greatest challenge in their transformation into therapeutics. Herein, we synthesized Fmoc-t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103602
更新日期：2020-05-20 00:00:00
abstract：:The G protein-coupled cannabinoid receptors type 1 (CB1R) and type 2 (CB2R), and their endocannabinoid (eCBs) ligands, have been implicated in several aspects of brain wiring during development. Here we aim to assess whether interfering with CB1R affects development, neuritogenesis and pathfinding of GnRH and AgRP neu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010168
更新日期：2019-12-25 00:00:00
abstract：:Growing interest and recent evidence have identified long non-coding RNA (lncRNA) as the potential regulatory elements for eukaryotes. LncRNAs can activate various transcriptional and post-transcriptional events that impact cellular functions though multiple regulatory functions. Recently, a large number of lncRNAs ha...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21082659
更新日期：2020-04-11 00:00:00
abstract：:Cell death represents a basic biological paradigm that governs outcomes and long-term sequelae in almost every hepatic disease. Necroptosis is a common form of programmed cell death in the liver. Necroptosis can be activated by ligands of death receptors, which then interact with receptor-interactive protein kinases 1...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms22010066
更新日期：2020-12-23 00:00:00
abstract：:Strawberry polyphenols have been extensively studied over the last two decades for their beneficial properties. Recently, their possible use in ameliorating skin conditions has also been proposed; however, their role in preventing UVA-induced damage in cosmetic formulation has not yet been investigated. Skin is consta...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160817870
更新日期：2015-08-04 00:00:00
abstract：:Drought stress is the major abiotic factor threatening maize (Zea mays L.) yield globally. Therefore, revealing the molecular mechanisms fundamental to drought tolerance in maize becomes imperative. Herein, we conducted a comprehensive comparative analysis of two maize inbred lines contrasting in drought stress tolera...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19103225
更新日期：2018-10-18 00:00:00
abstract：:Resveratrol, the phenolic substance isolated initially from Veratrum grandiflorum and richly present in grapes, wine, peanuts, soy, and berries, has been attracting attention of scientists and medical doctors for many decades. Herein, we review its effects on the vascular system. Studies utilizing cell cultures and pr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20071523
更新日期：2019-03-27 00:00:00
abstract：:The applications of whole-metagenome shotgun sequencing (WMGS) in routine clinical analysis are still limited. A combination of a DNA extraction procedure, sequencing, and bioinformatics tools is essential for the removal of human DNA and for improving bacterial species identification in a timely manner. We tackled th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18092011
更新日期：2017-09-20 00:00:00
abstract：:MicroRNAs (miRNAs) are short, non-coding post-transcriptional gene regulators. In mammalian cells, mature miRNAs are produced from primary precursors (pri-miRNAs) using canonical protein machinery, which includes Drosha/DGCR8 and Dicer, or the non-canonical mirtron pathway. In plant cells, mature miRNAs are excised fr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19124075
更新日期：2018-12-17 00:00:00
abstract：:Extracellular vesicles (EVs), including exosomes and microvesicles, are nano-to-micrometer vesicles released from nearly all cellular types. EVs comprise a mixture of bioactive molecules (e.g., mRNAs, miRNAs, lipids, and proteins) that can be transported to the targeted cells/tissues via the blood or lymph circulation...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21145163
更新日期：2020-07-21 00:00:00
abstract：:In this article, we present a method for the enhanced molecular dynamics simulation of protein and DNA systems called potential of mean force (PMF)-enriched sampling. The method uses partitions derived from the potentials of mean force, which we determined from DNA and protein structures in the Protein Data Bank (PDB)...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113405
更新日期：2018-10-30 00:00:00
abstract：:Genetic analysis of plants relies on high yields of pure DNA. For the strawberry tree (Arbutus unedo) this represents a great challenge since leaves can accumulate large amounts of polysaccharides, polyphenols and secondary metabolites, which co-purify with DNA. For this specie, standard protocols do not produce effic...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12064156
更新日期：2011-01-01 00:00:00
abstract：:We have previously reported that Amadori compounds exert anti-diabetic effects by lowering sucrose-induced hyperglycemia in normal Sprague-Dawley rats. In the present study we extended our recent findings to evaluate whether α-glucosidase inhibitor arginyl-fructose (AF) lowers blood glucose level in diabetic db/db mic...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15058352
更新日期：2014-05-12 00:00:00
abstract：:The risk of liver injury associated with the use of herbal medicinal products (HMPs) is well known among physicians caring for patients under a HMP therapy, as documented in case reports or case series and evidenced by using the Roussel Uclaf Causality Assessment Method (RUCAM) to verify a causal relationship. In many...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21145011
更新日期：2020-07-16 00:00:00
abstract：:Erythropoiesis is a complex process driving the production of red blood cells. During homeostasis, adult erythropoiesis takes place in the bone marrow and is tightly controlled by erythropoietin (EPO), a central hormone mainly produced in renal EPO-producing cells. The expression of EPO is strictly regulated by local ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218131
更新日期：2020-10-30 00:00:00
abstract：:The circadian system is an endogenous timekeeping system that synchronizes physiology and behavior with the 24 h solar day. Mice with total deletion of the core circadian clock gene Bmal1 show circadian arrhythmicity, cognitive deficits, and accelerated age-dependent decline in adult neurogenesis as a consequence of i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041394
更新日期：2020-02-19 00:00:00
abstract：:5-Fluorouracil (5-FU) is an important chemotherapeutic agent for the systemic treatment of colorectal cancer (CRC), but its effectiveness against CRC is limited by increased 5-FU resistance caused by the hypoxic tumor microenvironment. The purpose of our study was to assess the feasibility of using quinacrine (QC) to ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184366
更新日期：2019-09-05 00:00:00
abstract：:A fundamental step for cell growth and differentiation is the cell adhesion. The purpose of this study was to determine the adhesion of different cell lineages, adipose derived stromal cells, osteoblasts, and gingival fibroblast to titanium and zirconia dental implants with different surface treatments. Primary cells ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21114110
更新日期：2020-06-09 00:00:00
abstract：:Inflammation is a physiological mechanism used by organisms to defend themselves against infection, restoring homeostasis in damaged tissues. It represents the starting point of several chronic diseases such as asthma, skin disorders, cancer, cardiovascular syndrome, arthritis, and neurological diseases. An increasing...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19092714
更新日期：2018-09-11 00:00:00
abstract：:The centromere, in which kinetochore proteins are assembled, plays an important role in the accurate congression and segregation of chromosomes during cell mitosis. Although the function of the centromere and kinetochore is conserved from monocentric to holocentric, the DNA sequences of the centromere and components o...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235823
更新日期：2019-11-20 00:00:00
abstract：:Diabetic cardiomyopathy (DCM) has emerged as a relevant cause of heart failure among the diabetic population. Defined as a cardiac dysfunction that develops in diabetic patients independently of other major cardiovascular risks factors, such as high blood pressure and coronary artery disease, the underlying cause of D...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20112833
更新日期：2019-06-11 00:00:00
abstract：:The improvement of the embryo culture media is of high relevance due to its influence on successful implantation rates, pregnancy, neonatal outcomes, and potential effects in adult life. The ideal conditions for embryo development are those naturally occurring in the female reproductive tract, i.e., the oviductal and ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20215305
更新日期：2019-10-24 00:00:00
abstract：:Lafora disease (LD, OMIM #254780) is a rare, recessively inherited neurodegenerative disease with adolescent onset, resulting in progressive myoclonus epilepsy which is fatal usually within ten years of symptom onset. The disease is caused by loss-of-function mutations in either of the two genes EPM2A (laforin) or EPM...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18081743
更新日期：2017-08-11 00:00:00
abstract：:Protein kinase is a novel therapeutic target for human diseases. The off-target and side effects of ATP-competitive inhibitors preclude them from the clinically relevant drugs. The compounds targeting the druggable allosteric sites outside the highly conversed ATP binding pocket have been identified as promising alter...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19010111
更新日期：2018-01-01 00:00:00
abstract：:Oral cancer is a common cancer with poor prognosis. We evaluated the expression of PBK/TOPK (PDZ-binding kinase/T-LAK cell-originated protein kinase) and its prognostic significance in oral cancer. PBK/TOPK expression was measured by immunohistochemical staining of samples from 287 patients with oral cancer. The assoc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17071007
更新日期：2016-06-24 00:00:00
abstract：:The analgesic effect of venlafaxine (VLX), which is a selective serotonin and noradrenaline reuptake inhibitor (SNRI), has been observed on oxaliplatin-induced neuropathic pain in mice. Significant allodynia was shown after oxaliplatin treatment (6 mg/kg, i.p.); acetone and von Frey hair tests were used to assess cold...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071652
更新日期：2019-04-03 00:00:00
abstract：:The in vitro maturation of oocytes is frequently used as an assisted reproductive technique (ART), and has been successfully established in humans and rodents. To overcome the limitations of ART, novel procedures for the in vitro maturation of early follicles are emerging. During the follicle isolation procedure, the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051133
更新日期：2019-03-06 00:00:00
abstract：:Multifactorial metabolic diseases, such as non-alcoholic fatty liver disease, are a major burden to modern societies, and frequently present with no clearly defined molecular biomarkers. Herein we used system medicine approaches to decipher signatures of liver fibrosis in mouse models with malfunction in genes from un...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020832
更新日期：2021-01-15 00:00:00
