Heat Shock Cognate 70 Functions as A Chaperone for the Stability of Kinetochore Protein CENP-N in Holocentric Insect Silkworms.

Abstract:

:The centromere, in which kinetochore proteins are assembled, plays an important role in the accurate congression and segregation of chromosomes during cell mitosis. Although the function of the centromere and kinetochore is conserved from monocentric to holocentric, the DNA sequences of the centromere and components of the kinetochore are varied among different species. Given the lack of core centromere protein A (CENP-A) and CENP-C in the lepidopteran silkworm Bombyx mori, which possesses holocentric chromosomes, here we investigated the role of CENP-N, another important member of the centromere protein family essential for kinetochore assembly. For the first time, cellular localization and RNA interference against CENP-N have confirmed its kinetochore function in silkworms. To gain further insights into the regulation of CENP-N in the centromere, we analyzed the affinity-purified complex of CENP-N by mass spectrometry and identified 142 interacting proteins. Among these factors, we found that the chaperone protein heat shock cognate 70 (HSC70) is able to regulate the stability of CENP-N by prohibiting ubiquitin-proteasome pathway, indicating that HSC70 could control cell cycle-regulated degradation of CENP-N at centromeres. Altogether, the present work will provide a novel clue to understand the regulatory mechanism for the kinetochore activity of CENP-N during the cell cycle.

journal_name

Int J Mol Sci

authors

Li B,Li Z,Lu C,Chang L,Zhao D,Shen G,Kusakabe T,Xia Q,Zhao P

doi

10.3390/ijms20235823

subject

Has Abstract

pub_date

2019-11-20 00:00:00

issue

23

issn

1422-0067

pii

ijms20235823

journal_volume

20

pub_type

杂志文章
  • Aβ Beyond the AD Pathology: Exploring the Structural Response of Membranes Exposed to Nascent Aβ Peptide.

    abstract::The physiological and pathological roles of nascent amyloid beta (Aβ) monomers are still debated in the literature. Their involvement in the pathological route of Alzheimer's Disease (AD) is currently considered to be the most relevant, triggered by their aggregation into structured oligomers, a toxic species. Recentl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21218295

    authors: Rondelli V,Salmona M,Colombo L,Fragneto G,Fadda GC,Cantu' L,Del Favero E

    更新日期:2020-11-05 00:00:00

  • TATA-Like Boxes in RNA Polymerase III Promoters: Requirements for Nucleotide Sequences.

    abstract::tRNA and some other non-coding RNA genes are transcribed by RNA polymerase III (pol III), due to the presence of intragenic promoter, consisting of boxes A and B spaced by 30-40 bp. Such pol III promoters, called type 2, are also intrinsic to Short Interspersed Elements (SINEs). The contribution of 5'-flanking sequenc...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21103706

    authors: Tatosyan KA,Stasenko DV,Koval AP,Gogolevskaya IK,Kramerov DA

    更新日期:2020-05-25 00:00:00

  • Chronic Sildenafil Treatment Improves Vasomotor Function in a Mouse Model of Accelerated Aging.

    abstract::Aging leads to a loss of vasomotor control. Both vasodilation and vasoconstriction are affected. Decreased nitric oxide-cGMP-mediated relaxation is a hallmark of aging. It contributes to vascular disease, notably hypertension, infarction, and dementia. Decreased vasodilation can be caused by aging independently from c...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21134667

    authors: Golshiri K,Ataei Ataabadi E,Brandt R,van der Pluijm I,de Vries R,Danser J,Roks A

    更新日期:2020-06-30 00:00:00

  • The Role of Chemokines in Wound Healing.

    abstract::Wound healing is a multistep process with four overlapping but distinct stages: hemostasis, inflammation, proliferation, and remodeling. An alteration at any stage may lead to the development of chronic non-healing wounds or excessive scar formation. Impaired wound healing presents a significant health and economic bu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19103217

    authors: Ridiandries A,Tan JTM,Bursill CA

    更新日期:2018-10-18 00:00:00

  • The Differential Distribution of RAPTA-T in Non-Invasive and Invasive Breast Cancer Cells Correlates with Its Anti-Invasive and Anti-Metastatic Effects.

    abstract::Nanoscale secondary ion mass spectrometry (NanoSIMS) combined with transmission electron microscopy (TEM) can be a powerful approach to visualize the exact distribution of drugs at the sub-cellular level. In this work, we exploit this approach to identify the distribution and localisation of the organometallic rutheni...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18091869

    authors: Lee RFS,Escrig S,Maclachlan C,Knott GW,Meibom A,Sava G,Dyson PJ

    更新日期:2017-08-29 00:00:00

  • TPX2 is a prognostic marker and contributes to growth and metastasis of human hepatocellular carcinoma.

    abstract::Targeting protein for Xenopus kinesin-like protein 2 (TPX2), a microtubule-associated protein, impacts spindle assembly in human cells. Several studies have demonstrated that TPX2 is overexpressed in different types of human cancers and promotes tumor growth and metastasis. In this study, we found that the expression ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms151018148

    authors: Huang Y,Guo W,Kan H

    更新日期:2014-10-09 00:00:00

  • Construction of a Lectin-Glycan Interaction Network from Enterohemorrhagic Escherichia coli Strains by Multi-omics Analysis.

    abstract::Enterohemorrhagic Escherichia coli (EHEC) causes hemorrhagic colitis and hemolytic uremic syndrome. EHEC infection begins with bacterial adherence to the host intestine via lectin-like adhesins that bind to the intestinal wall. However, EHEC-related lectin-glycan interactions (LGIs) remain unknown. Here, we conducted ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21082681

    authors: Cho SH,Lee KM,Kim CH,Kim SS

    更新日期:2020-04-12 00:00:00

  • Detection and Management of Mango Dieback Disease in the United Arab Emirates.

    abstract::Mango is affected by different decline disorders causing significant losses to mango growers. In the United Arab Emirates (UAE), the pathogen was isolated from all tissues sampled from diseased trees affected by Lasiodiplodia theobromae. Symptoms at early stages of the disease included general wilting appearance of ma...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18102086

    authors: Saeed EE,Sham A,AbuZarqa A,A Al Shurafa K,S Al Naqbi T,Iratni R,El-Tarabily K,F AbuQamar S

    更新日期:2017-10-20 00:00:00

  • Investigation of Bone Growth in Additive-Manufactured Pedicle Screw Implant by Using Ti-6Al-4V and Bioactive Glass Powder Composite.

    abstract::In this study, we optimized the geometry and composition of additive-manufactured pedicle screws. Metal powders of titanium-aluminum-vanadium (Ti-6Al-4V) were mixed with reactive glass-ceramic biomaterials of bioactive glass (BG) powders. To optimize the geometry of pedicle screws, we applied a novel numerical approac...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21207438

    authors: Lam TN,Trinh MG,Huang CC,Kung PC,Huang WC,Chang W,Amalia L,Chin HH,Tsou NT,Shih SJ,Chen SY,Wang CC,Tsai PI,Wu MH,Huang EW

    更新日期:2020-10-09 00:00:00

  • The Efficacy of Amifostine against Multiple-Dose Doxorubicin-Induced Toxicity in Rats.

    abstract::Amifostine is well known cytoprotector which is efficient when administered before a wide range of antineoplastic agents. The aim of our study was to investigate amifostine effects on doxorubicin-induced toxic changes in rats. Amifostine (75 mg/kg ip) was given 30 min before each dose of doxorubicin (cumulatively 20 m...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19082370

    authors: Jaćević V,Dragojević-Simić V,Tatomirović Ž,Dobrić S,Bokonjić D,Kovačević A,Nepovimova E,Vališ M,Kuča K

    更新日期:2018-08-12 00:00:00

  • Isolation and characterization of 21 microsatellite loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an important economic plant in China.

    abstract::Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild ind...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms13021437

    authors: Li R,Yang J,Yang J,Dao Z

    更新日期:2012-01-01 00:00:00

  • An Updated View of Translocator Protein (TSPO).

    abstract::Decades of study on the role of mitochondria in living cells have evidenced the importance of the 18 kDa mitochondrial translocator protein (TSPO), first discovered in the 1977 as an alternative binding site for the benzodiazepine diazepam in the kidneys. This protein participates in a variety of cellular functions, i...

    journal_title:International journal of molecular sciences

    pub_type: 社论

    doi:10.3390/ijms18122640

    authors: Denora N,Natile G

    更新日期:2017-12-06 00:00:00

  • Immunogenomic Classification of Colorectal Cancer and Therapeutic Implications.

    abstract::The immune system has a substantial effect on colorectal cancer (CRC) progression. Additionally, the response to immunotherapeutics and conventional treatment options (e.g., chemotherapy, radiotherapy and targeted therapies) is influenced by the immune system. The molecular characterization of colorectal cancer (CRC) ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18102229

    authors: Roelands J,Kuppen PJK,Vermeulen L,Maccalli C,Decock J,Wang E,Marincola FM,Bedognetti D,Hendrickx W

    更新日期:2017-10-24 00:00:00

  • Fanconi-Bickel Syndrome: A Review of the Mechanisms That Lead to Dysglycaemia.

    abstract::Accumulation of glycogen in the kidney and liver is the main feature of Fanconi-Bickel Syndrome (FBS), a rare disorder of carbohydrate metabolism inherited in an autosomal recessive manner due to SLC2A2 gene mutations. Missense, nonsense, frame-shift (fs), in-frame indels, splice site, and compound heterozygous varian...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21176286

    authors: Sharari S,Abou-Alloul M,Hussain K,Ahmad Khan F

    更新日期:2020-08-31 00:00:00

  • Effect of Water Chemistry on Antimony Removal by Chemical Coagulation: Implications of ζ-Potential and Size of Precipitates.

    abstract::The process of coagulation and precipitation affect the fate and mobility of antimony (Sb) species in drinking water. Moreover, the solubility and physico-chemical properties of the precipitates may be affected by the media chemistry. Accordingly, the present study aimed to investigate the removal of Sb(III, V) specie...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20122945

    authors: Inam MA,Khan R,Akram M,Khan S,Yeom IT

    更新日期:2019-06-17 00:00:00

  • Glycomics Microarrays Reveal Differential In Situ Presentation of the Biofilm Polysaccharide Poly-N-acetylglucosamine on Acinetobacter baumannii and Staphylococcus aureus Cell Surfaces.

    abstract::The biofilm component poly-N-acetylglucosamine (PNAG) is an important virulence determinant in medical-device-related infections caused by ESKAPE group pathogens including Gram-positive Staphylococcus aureus and Gram-negative Acinetobacter baumannii. PNAG presentation on bacterial cell surfaces and its accessibility f...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21072465

    authors: Flannery A,Le Berre M,Pier GB,O'Gara JP,Kilcoyne M

    更新日期:2020-04-02 00:00:00

  • Impact of Natural Occurring ERAP1 Single Nucleotide Polymorphisms within miRNA-Binding Sites on HCMV Infection.

    abstract::Human cytomegalovirus (HCMV) is a β-herpesvirus that causes serious problems in people with a compromised immune system, whereas it coexists asymptomatically within the host with a healthy immune system. Like other viruses, HCMV has adopted multiples strategies to manipulate the host's immune responses. Among them, ex...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21165861

    authors: Melaiu O,D'Amico S,Tempora P,Lucarini V,Fruci D

    更新日期:2020-08-15 00:00:00

  • Multi-Omics Integration Highlights the Role of Ubiquitination in CCl4-Induced Liver Fibrosis.

    abstract::Liver fibrosis is the excessive accumulation of extracellular matrix proteins that occurs in chronic liver disease. Ubiquitination is a post-translational modification that is crucial for a plethora of physiological processes. Even though the ubiquitin system has been implicated in several human diseases, the role of ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21239043

    authors: Mercado-Gómez M,Lopitz-Otsoa F,Azkargorta M,Serrano-Maciá M,Lachiondo-Ortega S,Goikoetxea-Usandizaga N,Rodríguez-Agudo R,Fernández-Ramos D,Bizkarguenaga M,Juan VG,Lectez B,Aloria K,Arizmendi JM,Simon J,Alonso C,Lozano JJ,

    更新日期:2020-11-27 00:00:00

  • Metabolic Effects of Metformin in the Failing Heart.

    abstract::Accumulating evidence shows that metformin is an insulin-sensitizing antidiabetic drug widely used in the treatment of type 2 diabetes mellitus (T2DM), which can exert favorable effects on cardiovascular risk and may be safely used in patients with heart failure (HF), and even able to reduce the incidence of HF and to...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19102869

    authors: Dziubak A,Wójcicka G,Wojtak A,Bełtowski J

    更新日期:2018-09-21 00:00:00

  • The Role of Exosomes in Pancreatic Cancer.

    abstract::Pancreatic cancer remains one of the deadliest cancers in the world, as a consequence of late diagnosis, early metastasis and limited response to chemotherapy, under which conditions the potential mechanism of pancreatic cancer progression requires further study. Exosomes are membrane vesicles which are important in t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20184332

    authors: Lan B,Zeng S,Grützmann R,Pilarsky C

    更新日期:2019-09-04 00:00:00

  • Stress Marks on the Genome: Use or Lose?

    abstract::Oxidative stress and the resulting damage to DNA are inevitable consequence of endogenous physiological processes further amplified by cellular responses to environmental exposures. If left unrepaired, oxidative DNA lesions can block essential processes such as transcription and replication or can induce mutations. Em...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20020364

    authors: Bokhari B,Sharma S

    更新日期:2019-01-16 00:00:00

  • Application of novel polymorphic microsatellite loci identified in the Korean Pacific Abalone (Haliotis diversicolor supertexta (Haliotidae)) in the genetic characterization of wild and released populations.

    abstract::The small abalone, Haliotis diversicolor supertexta, of the family Haliotidae, is one of the most important species of marine shellfish in eastern Asia. Over the past few decades, this species has drastically declined in Korea. Thus, hatchery-bred seeds have been released into natural coastal areas to compensate for t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms130910750

    authors: An HS,Lee JW,Hong SW

    更新日期:2012-01-01 00:00:00

  • Vortioxetine Subchronically Activates Serotonergic Transmission via Desensitization of Serotonin 5-HT1A Receptor with 5-HT3 Receptor Inhibition in Rats.

    abstract::Vortioxetine is a novel, multimodal antidepressant with unique targets, including the inhibition of the serotonin transporter (SET), of serotonin 5-HT3 (5-HT3R), and of 5-HT7 (5-HT7R) receptors and partial agonism to serotonin 5-HT1A (5-HT1AR) receptors in humans. Vortioxetine has a lower affinity to 5-HT1AR and 5-HT7...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20246235

    authors: Okada M,Okubo R,Fukuyama K

    更新日期:2019-12-10 00:00:00

  • Chromatin Manipulation and Editing: Challenges, New Technologies and Their Use in Plants.

    abstract::An ongoing challenge in functional epigenomics is to develop tools for precise manipulation of epigenetic marks. These tools would allow moving from correlation-based to causal-based findings, a necessary step to reach conclusions on mechanistic principles. In this review, we describe and discuss the advantages and li...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms22020512

    authors: Fal K,Tomkova D,Vachon G,Chabouté ME,Berr A,Carles CC

    更新日期:2021-01-06 00:00:00

  • Autologous Mesenchymal Stroma Cells Are Superior to Allogeneic Ones in Bone Defect Regeneration.

    abstract::The application of autologous mesenchymal stem cells (MSC) for the treatment of bone defects requires two invasive procedures and several weeks of ex vivo cell expansion. To overcome these limitations, the administration of allogeneic MSC may be attractive, because they are anticipated to be immunoprivileged. Because ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19092526

    authors: Rapp AE,Bindl R,Erbacher A,Kruchen A,Rojewski M,Schrezenmeier H,Müller I,Ignatius A

    更新日期:2018-08-25 00:00:00

  • Hydroxylation of Antitubercular Drug Candidate, SQ109, by Mycobacterial Cytochrome P450.

    abstract::Spreading of the multidrug-resistant (MDR) strains of the one of the most harmful pathogen Mycobacterium tuberculosis (Mtb) generates the need for new effective drugs. SQ109 showed activity against resistant Mtb and already advanced to Phase II/III clinical trials. Fast SQ109 degradation is attributed to the human liv...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21207683

    authors: Bukhdruker S,Varaksa T,Grabovec I,Marin E,Shabunya P,Kadukova M,Grudinin S,Kavaleuski A,Gusach A,Gilep A,Borshchevskiy V,Strushkevich N

    更新日期:2020-10-16 00:00:00

  • Mechanistic investigation of ROS-induced DNA damage by oestrogenic compounds in lymphocytes and sperm using the comet assay.

    abstract::Past research has demonstrated that oestrogenic compounds produce strand breaks in the DNA of sperm and lymphocytes via reactive oxygen species (ROS). In the current investigation, sperm and lymphocytes were treated in vitro with oestrogenic compounds (diethylstilboestrol, progesterone, 17β-oestradiol, noradrenaline a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms12052783

    authors: Cemeli E,Anderson D

    更新日期:2011-01-01 00:00:00

  • Tunneling Nanotubes-Mediated Protection of Mesenchymal Stem Cells: An Update from Preclinical Studies.

    abstract::Tunneling nanotubes (TNTs) are thin membrane elongations among the cells that mediate the trafficking of subcellular organelles, biomolecules, and cues. Mesenchymal stem cells (MSCs) receive substantial attention in tissue engineering and regenerative medicine. Many MSCs-based clinical trials are ongoing for dreadful ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21103481

    authors: Soundara Rajan T,Gugliandolo A,Bramanti P,Mazzon E

    更新日期:2020-05-14 00:00:00

  • Effect of asafoetida extract on growth and quality of Pleurotus ferulic.

    abstract::Different concentrations of asafoetida extract were added to the medium of Pleurotus ferulic and the effects of the extract on growth of P. ferulic mycelium and fruiting bodies was observed. As the amount of asafoetida extract additive was increased, the growth of Pleurotus mycelium was faster, the time formation of b...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms11010041

    authors: Feng Z,Bai Y,Lu F,Huang W,Li X,Hu X

    更新日期:2009-12-29 00:00:00

  • Effects of Pup Separation on Stress Response in Postpartum Female Rats.

    abstract::There is a complex collection of neuroendocrine function during the postpartum period. Prolactin (PRL) released by suckling stimulus and its PRL receptors (PRL-R) in the central nervous system (CNS) are involved in hyporesponsiveness of the hypothalamic-pituitary-adrenal (HPA) axis in lactating mammals including roden...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18071370

    authors: Kalyani M,Callahan P,Janik JM,Shi H

    更新日期:2017-06-27 00:00:00